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Entry
EC 4.2.1.84                 Enzyme                                 

Name
nitrile hydratase;
nitrilase (ambiguous);
3-cyanopyridine hydratase;
NHase;
L-NHase;
H-NHase;
acrylonitrile hydratase;
aliphatic nitrile hydratase;
nitrile hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
aliphatic-amide hydro-lyase (nitrile-forming)
Reaction(IUBMB)
an aliphatic amide = a nitrile + H2O [RN:R02826]
Reaction(KEGG)
Substrate
aliphatic amide [CPD:C02244]
Product
nitrile [CPD:C00726];
H2O [CPD:C00001]
Comment
Acts on short-chain aliphatic nitriles, converting them into the corresponding amides. Does not act on these amides or on aromatic nitriles. cf. EC 3.5.5.1 nitrilase.
History
EC 4.2.1.84 created 1989
Pathway
ec00364  Fluorobenzoate degradation
ec00380  Tryptophan metabolism
ec00627  Aminobenzoate degradation
ec00643  Styrene degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K01721  nitrile hydratase subunit alpha
K20807  nitrile hydratase subunit beta
Genes
MBR: MONBRDRAFT_37534
SRE: PTSG_03349
SMIN: v1.2.038621.t4(symbB.v1.2.038621.t4)
FCY: FRACYDRAFT_162294
AAF: AURANDRAFT_25066(NHY)
EHX: EMIHUDRAFT_449479
KPE: KPK_2672(nthA) KPK_2673(nthB)
KVA: Kvar_2628 Kvar_2629
KPK: A593_23230 A593_23235
KVD: KR75_21025 KR75_21030
PVA: Pvag_pPag20175(nthA) Pvag_pPag20176(nthB)
PPUU: PputUW4_03351(nthA) PputUW4_03352(nthB)
ACI: ACIAD1615(nthB) ACIAD1616(nthA)
RSL: RPSI07_2728(nthB) RPSI07_2729(nthA)
PARB: CJU94_26475(nthA) CJU94_26480(nthB) CJU94_30830(nthA) CJU94_30835(nthB)
PHS: C2L64_19570(nthA) C2L64_19575(nthB) C2L64_28135(nthB) C2L64_28140(nthA)
PTER: C2L65_17445(nthA) C2L65_17450(nthB) C2L65_20370(nthA) C2L65_20375(nthB)
PGP: CUJ91_33760(nthB) CUJ91_33765(nthA)
BPT: Bpet1415(nthB) Bpet1416(nthA)
BOZ: DBV39_05125(nthA) DBV39_05130(nthB)
VBO: CKY39_11435(nthA) CKY39_11440(nthB)
LIH: L63ED372_00221(nhhA) L63ED372_00222(nthB)
HSE: Hsero_0931(nthB) Hsero_0932(nthA)
HRB: Hrubri_2877(nthA) Hrubri_2878(nthB)
AMIH: CO731_02859(nhhA) CO731_02860(nthB)
SME: SMc01423(nthB) SMc01424(nthA)
SMX: SM11_chr1213(nthA) SM11_chr1214(nthB)
SMI: BN406_01927(nthB) BN406_01929(nthA)
SMEL: SM2011_c01423(nthB) SM2011_c01424(nthA)
RHI: NGR_c20110(nthB) NGR_c20120(nthA)
SFH: SFHH103_01944(nthB) SFHH103_01945(nthA)
SFD: USDA257_c44270(nthB) USDA257_c44280(nthA)
EAD: OV14_3491(nthB) OV14_3492(nthA)
ARA: Arad_2889(nthB) Arad_2890(nthA)
AGC: BSY240_266(nthA) BSY240_267(nthB)
RET: RHE_CH02782(nthB) RHE_CH02783(nthA)
REL: REMIM1_CH02825(nthB) REMIM1_CH02826(nthA)
REP: IE4803_CH02918(nthB) IE4803_CH02919(nthA)
RLE: RL3238(nhb2) RL3239(nthA) pRL110124 pRL110125
RIR: BN877_p0387(nthB) BN877_p0388(nthA)
RHL: LPU83_2666(nthB) LPU83_2667(nthA)
RGA: RGR602_CH02432(nthB) RGR602_CH02433(nthA)
RHN: AMJ98_CH02944(nthB) AMJ98_CH02945(nthA)
RPHA: AMC79_CH02936(nthB) AMC79_CH02937(nthA)
RHT: NT26_2092(nthB) NT26_2093(nthA)
RHX: AMK02_CH02873(nthB) AMK02_CH02874(nthA)
BJA: bll4497(nthB) bll4498(nthA)
BRA: BRADO3845(nthA) BRADO3846(nthB)
BBT: BBta_4084(nthB) BBta_4085(nthA)
BRS: S23_36770(nthA) S23_36780(nthB)
BRO: BRAD285_3526(nthB) BRAD285_3527(nthA)
BRK: CWS35_25115(nthA) CWS35_25120(nthB)
BOT: CIT37_31440(nthA) CIT37_31445(nthB)
RPA: RPA2805(nthA) RPA2806(nthB)
BOS: BSY19_44(nthA) BSY19_45(nthB)
MEE: DA075_23830(nthA) DA075_23835(nthB)
RVA: Rvan_0450
BRN: D1F64_20135(nthA) D1F64_20140(nthB)
SIL: SPO1314(nthA) SPO1315(nthB)
RDE: RD1_1908(nthA) RD1_1909(nthB)
PZH: CX676_10550(nthB) CX676_10555(nthA)
PARO: CUV01_02880(nthB) CUV01_02885(nthA)
DSH: Dshi_1849(nthA)
PGA: PGA1_c23870(nthB) PGA1_c23880(nthA)
PGL: PGA2_c21890(nthB) PGA2_c21900(nthA)
PHP: PhaeoP97_02255(nthB) PhaeoP97_02256(nthA)
PPIC: PhaeoP14_02175(nthB) PhaeoP14_02176(nthA)
OTM: OSB_19880(nthB_1) OSB_19890(nthB_2) OSB_19900
LABR: CHH27_01065(nthB) CHH27_01070(nthA)
SULZ: C1J03_16150(nthB) C1J03_16155(nthA)
SULI: C1J05_05460(nthA) C1J05_05465(nthB)
RBG: BG454_15860(nthB) BG454_15865(nthA)
THAS: C6Y53_01370(nthB) C6Y53_01375(nthA)
SPHR: BSY17_2185(nthB) BSY17_2186(nthA)
ACR: Acry_1077
TMO: TMO_0454(nthB) TMO_0455(nthA) TMO_b0395(nthA) TMO_b0396
RHA: RHA1_ro00360(nthA) RHA1_ro00361(nthB)
RER: RER_59240(nha1) RER_59250(nha2)
RQI: C1M55_30330(nthA) C1M55_30335(nthB)
GPO: GPOL_c06980(nthA) GPOL_c06990(nthB)
AAU: AAur_pTC20145(nthB) AAur_pTC20146(nthA) AAur_pTC20147(nthB)
GOB: Gobs_2330
MMAR: MODMU_1179(nthA)
RXY: Rxyl_2315
AMR: AM1_1194(nthA) AM1_1195(nthB)
 » show all
Taxonomy
Reference
1
  Authors
Asano, Y., Fujishiro, K., Tani, Y. and Yamada, H.
  Title
Microbial degradation of nitrile compounds. 5. Aliphatic nitrile hydratase from Arthrobacter sp J-1. Purification and characterization.
  Journal
Agric Biol Chem 46:1165-1174 (1982)
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.84
IUBMB Enzyme Nomenclature: 4.2.1.84
ExPASy - ENZYME nomenclature database: 4.2.1.84
UM-BBD (Biocatalysis/Biodegradation Database): 4.2.1.84
BRENDA, the Enzyme Database: 4.2.1.84
CAS: 82391-37-5

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