KEGG   ENZYME: 4.2.1.93Help
Entry
EC 4.2.1.93                 Enzyme                                 

Name
ATP-dependent NAD(P)H-hydrate dehydratase;
reduced nicotinamide adenine dinucleotide hydrate dehydratase;
ATP-dependent H4NAD(P)+OH dehydratase;
(6S)-beta-6-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase(ATP-hydrolysing);
(6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase (ATP-hydrolysing;
NADH-forming)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine-dinucleotide hydro-lyase (ATP-hydrolysing; NADH-forming)
Reaction(IUBMB)
(1) ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH [RN:R00129];
(2) ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH [RN:R10288]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide [CPD:C04856];
(6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate [CPD:C04899]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
NADH [CPD:C00004];
NADPH [CPD:C00005]
Comment
Acts equally well on hydrated NADH and hydrated NADPH. NAD(P)H spontaneously hydrates to both the (6S)- and (6R)- isomers, and these are interconverted by EC 5.1.99.6, NAD(P)H-hydrate epimerase, to a 60:40 ratio [4]. Hence EC 4.2.1.93 together with EC 5.1.99.6 can restore the mixture of hydrates into NAD(P)H [3,4]. The enzyme from eukaryotes has no activity with ADP, contrary to the enzyme from bacteria (cf. EC 4.2.1.136, ADP-dependent NAD(P)H-hydrate dehydratase) [4].
History
EC 4.2.1.93 created 1992, modified 2012
Orthology
K17757  ATP-dependent NAD(P)H-hydrate dehydratase
Genes
HSA: 55739(NAXD)
PTR: 452663(NAXD)
PPS: 100977905(NAXD)
GGO: 101150913(NAXD)
PON: 100173186(NAXD)
NLE: 100604464(NAXD)
MCC: 694722(NAXD)
MCF: 102128889(NAXD)
CSAB: 103214799(NAXD)
RRO: 104679240(NAXD)
RBB: 108527064(NAXD)
CJC: 100405542(NAXD)
SBQ: 101045356(CARKD)
MMU: 69225(Naxd)
RNO: 361185(Naxd)
CGE: 100767494(Naxd)
NGI: 103739906 103751678(Naxd)
HGL: 101717804(Naxd)
CCAN: 109687738(Naxd)
OCU: 100358583(NAXD)
TUP: 102481205(NAXD)
CFA: 607381(NAXD)
AML: 100479259(NAXD)
UMR: 103657622(NAXD)
ORO: 101380814(CARKD)
FCA: 101096534(NAXD)
PTG: 102954132(NAXD)
AJU: 106986360(NAXD)
BTA: 613996(NAXD)
BOM: 102284176(NAXD)
BIU: 109566961(NAXD)
PHD: 102342914(NAXD)
CHX: 102171618(NAXD)
OAS: 101116066(CARKD) 105606082
SSC: 100153456(NAXD)
CFR: 102507835(NAXD)
CDK: 105094105(NAXD)
BACU: 103009018(NAXD)
LVE: 103089164(NAXD)
OOR: 101275937(CARKD)
ECB: 100066349(NAXD)
EPZ: 103565641(NAXD)
EAI: 106838205(NAXD)
MYB: 102242541(NAXD)
MYD: 102771854(NAXD)
HAI: 109383911(NAXD)
RSS: 109447077(NAXD)
PALE: 102886483(NAXD)
LAV: 100653762(NAXD)
TMU: 101345818
MDO: 100023457(NAXD)
SHR: 100921239 100924303(NAXD)
OAA: 100084483(NAXD)
GGA: 418755(NAXD)
MGP: 100549983(NAXD)
CJO: 107307941(NAXD)
APLA: 101804240(NAXD)
ACYG: 106044453(CARKD)
TGU: 100219904(NAXD)
GFR: 102034719(NAXD)
FAB: 101817373(NAXD)
PHI: 102099739(NAXD)
PMAJ: 107201575(NAXD)
CCAE: 111944791(NAXD)
CCW: 104686126(NAXD)
FPG: 101910319(CARKD) 101923883(NAXD)
FCH: 102046376(NAXD)
CLV: 102088516(NAXD)
EGZ: 104132456(CARKD)
AAM: 106493918(NAXD)
ASN: 102367921(NAXD)
AMJ: 102559151(NAXD)
PSS: 102453397(NAXD)
CMY: 102941875(NAXD)
CPIC: 101943239(NAXD)
ACS: 100554347(naxd)
PVT: 110079338(NAXD)
PBI: 103060468(NAXD)
GJA: 107110343(NAXD)
XLA: 108708461(naxd.L) 108709737(naxd.S)
XTR: 549064(naxd)
NPR: 108787452(NAXD)
DRE: 797867(naxd)
CCAR: 109069423(naxd)
IPU: 108261034 108266299(naxd)
AMEX: 103035327(naxd)
TRU: 101070180(naxd)
LCO: 104932339(naxd)
NCC: 104955102(carkd)
OLA: 101163124(naxd)
XMA: 102234599(naxd)
PRET: 103459757(naxd)
NFU: 107389845(naxd)
KMR: 108232361(naxd)
CSEM: 103392143(naxd)
LCF: 108899727(naxd)
SDU: 111218097(naxd)
HCQ: 109524678(naxd)
BPEC: 110173162(naxd)
MALB: 109965384(naxd)
ELS: 105016496(naxd)
SFM: 108933210(naxd)
LCM: 102349649(NAXD)
CMK: 103177553(naxd)
CIN: 100183233
SPU: 586141
APLC: 110975932
SKO: 100371541
DME: Dmel_CG10424(CG10424)
DER: 6545338
DPE: 6593555
DSI: Dsimw501_GD12303(Dsim_GD12303)
MDE: 101891655
AAG: 5565418
AME: 550956
BIM: 100747542
BTER: 100648143
SOC: 105199404
AEC: 105149171
ACEP: 105626756
PBAR: 105427939
HST: 105187053
DQU: 106744267
CFO: 105249629
LHU: 105675374
PGC: 109854690
PCF: 106786341
NVI: 100120400
MDL: 103569008
TCA: 663809
DPA: 109537022
NVL: 108563047
PRAP: 110996938
API: 100159510
DNX: 107163478
CLEC: 106670247
ZNE: 110838069
FCD: 110855848
TUT: 107363089
CEL: CELE_R107.2(R107.2)
CBR: CBG06820
BMY: Bm1_07040
TSP: Tsp_05834
CRG: 105336406
MYI: 110454118
OBI: 106884448
SHX: MS3_07582
EGL: EGR_02260
EPA: 110233995
ADF: 107337387
AQU: 100636397
ATH: AT5G19150
CRB: 17883242
THJ: 104818802
CPAP: 110808283
CIT: 102608672
TCC: 18602914
GRA: 105796627
DZI: 111307930
EGR: 104424730
VRA: 106765509
VAR: 108342505
CCAJ: 109809615
CAM: 101494709
LJA: Lj6g3v1880130.1(Lj6g3v1880130.1)
AIP: 107638601
LANG: 109329796
FVE: 101310981
PPER: 18788705
PMUM: 103322821
PXB: 103930821
ZJU: 107423291
CSV: 101209583
CMO: 103483736
MCHA: 111009202
CMAX: 111479195
CMOS: 111457620
CPEP: 111791306
RCU: 8271588
JCU: 105631929
HBR: 110655219
POP: 7454254
JRE: 109005882
VVI: 100244161
SLY: 101252921
SPEN: 107008335
SOT: 102584947
CANN: 107841242
NSY: 104238446
SIND: 105171385
HAN: 110894085
LSV: 111900451
CCAV: 112516233
DCR: 108226051
BVG: 104888818
SOE: 110775306
NNU: 104596442
OSA: 4350275
DOSA: Os11t0276300-01(Os11g0276300)
OBR: 102702978
BDI: 100837614
ATS: 109743558(LOC109743558)
SBI: 8071812
ZMA: 100216749
SITA: 101782503
PDA: 103708561
EGU: 105038634
MUS: 103988678
DCT: 110095628
PEQ: 110027509
AOF: 109831733
ATR: 18448538
PPP: 112285318
MNG: MNEG_3436
APRO: F751_5687
SCE: YKL151C(NNR2)
ERC: Ecym_8245
NCS: NCAS_0F02350(NCAS0F02350)
NDI: NDAI_0C03850(NDAI0C03850)
TPF: TPHA_0E02390(TPHA0E02390)
TBL: TBLA_0A10100(TBLA0A10100)
TDL: TDEL_0E03700(TDEL0E03700)
KAF: KAFR_0E01710(KAFR0E01710)
CAL: CAALFM_C602030CA(CaO19.3508)
CAUR: QG37_04911
NCR: NCU02513
NTE: NEUTE1DRAFT126541(NEUTE1DRAFT_126541)
MGR: MGG_13834
SSCK: SPSK_04353
MAW: MAC_05252
MAJ: MAA_03978
CMT: CCM_06173
MBE: MBM_02219
ANI: AN5994.2
ANG: ANI_1_696144(An16g04910)
ABE: ARB_06465
TVE: TRV_07085
PTE: PTT_19720
SPO: SPCC61.03
CNE: CNL05250
CNB: CNBI1570
ABP: AGABI1DRAFT126077(AGABI1DRAFT_126077)
ABV: AGABI2DRAFT143608(AGABI2DRAFT_143608)
MGL: MGL_0838
DFA: DFA_08558
EHI: EHI_194450(46.t00021)
SMIN: v1.2.010210.t1(symbB.v1.2.010210.t1) v1.2.023417.t1(symbB.v1.2.023417.t1)
SPAR: SPRG_00224
 » show all
Taxonomy
Reference
1  [PMID:13331940]
  Authors
MEINHART JO, CHAYKIN S, KREBS EG.
  Title
Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.
  Journal
J Biol Chem 220:821-9 (1956)
  Sequence
[sce:YKL151C]
Reference
2
  Authors
Regueiro Varela, B., Amelunxen, R. and Grisolia, S.
  Title
Synthesis and degradation of monohydroxytetrahydronicotinamide adenine dinucleotide phosphate.
  Journal
Physiol Chem Phys 2:445-454 (1970)
Reference
3  [PMID:3061454]
  Authors
Acheson SA, Kirkman HN, Wolfenden R.
  Title
Equilibrium of 5,6-hydration of NADH and mechanism of ATP-dependent dehydration.
  Journal
Biochemistry 27:7371-5 (1988)
  Sequence
[sce:YKL151C]
Reference
4  [PMID:21994945]
  Authors
Marbaix AY, Noel G, Detroux AM, Vertommen D, Van Schaftingen E, Linster CL
  Title
Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair.
  Journal
J Biol Chem 286:41246-52 (2011)
DOI:10.1074/jbc.C111.310847
  Sequence
[mmu:69225] [sce:YKL151C]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.93
IUBMB Enzyme Nomenclature: 4.2.1.93
ExPASy - ENZYME nomenclature database: 4.2.1.93
BRENDA, the Enzyme Database: 4.2.1.93
CAS: 116669-08-0

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