KEGG   ENZYME: 4.2.2.10Help
Entry
EC 4.2.2.10                 Enzyme                                 

Name
pectin lyase;
pectin trans-eliminase;
endo-pectin lyase;
polymethylgalacturonic transeliminase;
pectin methyltranseliminase;
pectolyase;
PL;
PNL;
PMGL
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
(1->4)-6-O-methyl-alpha-D-galacturonan lyase
Reaction(IUBMB)
Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
Comment
Favours pectin, the methyl ester, over pectate, the anion (which is the preferred substrate of EC 4.2.2.2, pectate lyase). Demethylation progressively slows its action; it can nevertheless cleave on either side of a demethylated residue if the residue at the other end of the scissile bond is methylated.
History
EC 4.2.2.10 created 1972, modified 2002
Orthology
K01732  pectin lyase
K19551  pectate lyase C
Genes
MTM: MYCTH_52332
TMN: UCRPA7_7400
FGR: FGSG_01607 FGSG_03121 FGSG_03483 FGSG_13834
FPU: FPSE_02103 FPSE_02805 FPSE_09996 FPSE_10251
FVR: FVEG_03470 FVEG_05700 FVEG_09702 FVEG_12209 FVEG_13711
FOX: FOXG_05617 FOXG_11096 FOXG_13331 FOXG_16516
NHE: NECHADRAFT_106390 NECHADRAFT_122319 NECHADRAFT_16877 NECHADRAFT_30203 NECHADRAFT_44358 NECHADRAFT_78318 NECHADRAFT_82428
VAL: VDBG_02740 VDBG_04520 VDBG_05494 VDBG_08488 VDBG_09088
VDA: VDAG_03656 VDAG_07144 VDAG_07238 VDAG_08154 VDAG_08734
CFJ: CFIO01_02126 CFIO01_03438 CFIO01_05967 CFIO01_09235 CFIO01_09971
ANG: ANI_1_16034(An03g00190) ANI_1_1744134(An15g07160) ANI_1_30184(An19g00270) ANI_1_554094(An11g04030) ANI_1_624124(An14g04370)
ECA: ECA1499(pnl)
PATR: EV46_07595
PATO: GZ59_15370(pnl)
PCT: PC1_1371
PEC: W5S_1691
EAM: EAMY_0653(eop2)
EAY: EAM_2780(eop2)
PST: PSPTO_4101(hopAK1)
PSB: Psyr_3839
PSYR: N018_06330
PSP: PSPPH_1424(hopAK1) PSPPH_3992(pnlA)
PFS: PFLU_2293(pnl)
CJA: CJA_2914(pel3C)
SDE: Sde_3007(pel3C)
SCL: sce4938(yvpA)
BSU: BSU18650(pelB) BSU34950(pelC)
BSH: BSU6051_18650(pelB) BSU6051_34950(pelC)
BSUT: BSUB_02005(pelB) BSUB_03725(pelC)
BSUL: BSUA_02005(pelB) BSUA_03725(pelC)
BSS: BSUW23_09955(pelB) BSUW23_17130(pelC)
BSO: BSNT_10122(yvpA)
BSQ: B657_18650(pelB) B657_34950(pelC)
BSX: C663_1929(pelB) C663_3384(pelC)
BLI: BL00361(pelB) BL03597(yvpA)
BLD: BLi03053(pelB) BLi03741(pelC)
BLH: BaLi_c31330(pelB) BaLi_c37440(pelC)
BAY: RBAM_036320(pelB)
BAQ: BACAU_3655(pelB)
BYA: BANAU_3815(pelB)
BAMP: B938_18645
BAML: BAM5036_3554(pelB)
BAMA: RBAU_3765(pelB)
BAMN: BASU_3548(pelB)
BAMB: BAPNAU_3827(pelB)
BAMT: AJ82_20540
BAMY: V529_39070(pelB)
BMP: NG74_03810(pelB)
BAO: BAMF_3745(pelB)
BAZ: BAMTA208_19805(pelB)
BQL: LL3_04068(pelB)
BXH: BAXH7_04063(pelB)
BQY: MUS_4319(pelB)
BAMI: KSO_000885
BAMC: U471_37900
BAMF: U722_19355
BACP: SB24_10585
BACB: OY17_01825
BACL: BS34A_20660(pelB) BS34A_38050(pelC)
BGY: BGLY_4139(pelC)
PPM: PPSC2_04665(yvpA) PPSC2_07120(pelB)
PPO: PPM_0875(yvpA) PPM_1351(pelB)
PMS: KNP414_00430(pelA)
PMW: B2K_01935
RCH: RUM_06550
 » show all
Taxonomy
Reference
1
  Authors
Albersheim, P., Neukom, H. and Deuel, H.
  Title
Uber die Bildung von ungesattigten Abbauprodukten durch ein pekinabbauendes Enzym.
  Journal
Helv Chim Acta 43:1422-1426 (1960)
Reference
2  [PMID:9195887]
  Authors
Mayans O, Scott M, Connerton I, Gravesen T, Benen J, Visser J, Pickersgill R, Jenkins J.
  Title
Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases.
  Journal
Structure 5:677-89 (1997)
DOI:10.1016/S0969-2126(97)00222-0
  Sequence
Reference
3
  Authors
Kester, H.C.M and Visser, J.
  Title
Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger.
  Journal
FEMS Microbiol Lett 120:63-68 (1994)
Reference
4  [PMID:12110197]
  Authors
Mutenda KE, Korner R, Christensen TM, Mikkelsen J, Roepstorff P.
  Title
Application of mass spectrometry to determine the activity and specificity of pectin lyase A.
  Journal
Carbohydr Res 337:1217-27 (2002)
DOI:10.1016/S0008-6215(02)00127-1
Other DBs
ExplorEnz - The Enzyme Database: 4.2.2.10
IUBMB Enzyme Nomenclature: 4.2.2.10
ExPASy - ENZYME nomenclature database: 4.2.2.10
BRENDA, the Enzyme Database: 4.2.2.10
CAS: 9033-35-6

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