KEGG   ENZYME: 4.2.2.5Help
Entry
EC 4.2.2.5                  Enzyme                                 

Name
chondroitin AC lyase;
chondroitinase (ambiguous);
chondroitin sulfate lyase;
chondroitin AC eliminase;
chondroitinase AC;
ChnAC
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
chondroitin AC lyase
Reaction(IUBMB)
Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
Reaction(KEGG)
(other) R10870 R10872
Show
Comment
Acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate. In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta1-3)GalNAc(beta1-]n, which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha1-3)GalNAc(beta1-]n of DS. Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source [4].
History
EC 4.2.2.5 created 1972 (EC 4.2.99.6 created 1965, part incorporated 1976)
Orthology
K19049  chondroitin AC lyase
Genes
LCP: LC55x_3170
VVU: VV2_1090 VV2_1110
VVY: VVA1613 VVA1636
VVM: VVMO6_04481 VVMO6_04502
VVL: VV93_v1c44830 VV93_v1c45010
VNA: PN96_04575 PN96_15915
VEJ: VEJY3_08795 VEJY3_11250 VEJY3_24021
VNI: VIBNI_A2386 VIBNI_B0676
VMI: AL543_05760
PSP: PSPPH_1510
SALH: HMF8227_02592(cslA)
DGG: DGI_2110
PRI: PRIO_0266
SMAL: SMALA_8146
SRO: Sros_7449
AMI: Amir_0500
OTE: Oter_4325
BTH: BT_2964
BTHO: Btheta7330_00132(cslA)
BFR: BF1585
BCEL: BcellWH2_00910(cslA_1) BcellWH2_01185(cslA_2) BcellWH2_02524(cslA_3) BcellWH2_03147(cslA_4) BcellWH2_03149(cslA_5)
PSAC: PSM36_3149
ASH: AL1_00280
MBAS: ALGA_3393
PHE: Phep_0786
ELB: VO54_03456(cslA_1) VO54_03457(cslA_2)
TMAR: MARIT_2107(cslA)
 » show all
Taxonomy
Reference
1  [PMID:13727579]
  Authors
NAKADA HI, WOLFE JB.
  Title
Studies on the enzyme chondroitinase: product structure and ion effects.
  Journal
Arch Biochem Biophys 94:244-51 (1961)
DOI:10.1016/0003-9861(61)90037-6
Reference
2  [PMID:11500043]
  Authors
Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R.
  Title
Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum.
  Journal
Biochem Biophys Res Commun 286:343-51 (2001)
DOI:10.1006/bbrc.2001.5380
Reference
3  [PMID:9761894]
  Authors
Fethiere J, Shilton BH, Li Y, Allaire M, Laliberte M, Eggimann B, Cygler M.
  Title
Crystallization and preliminary analysis of chondroitinase AC from Flavobacterium heparinum.
  Journal
Acta Crystallogr D Biol Crystallogr 54 ( Pt 2):279-80 (1998)
  Sequence
[phe:Phep_0786]
Reference
4  [PMID:16336265]
  Authors
Huckerby TN, Nieduszynski IA, Giannopoulos M, Weeks SD, Sadler IH, Lauder RM.
  Title
Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides.
  Journal
FEBS J 272:6276-86 (2005)
DOI:10.1111/j.1742-4658.2005.05009.x
Other DBs
ExplorEnz - The Enzyme Database: 4.2.2.5
IUBMB Enzyme Nomenclature: 4.2.2.5
ExPASy - ENZYME nomenclature database: 4.2.2.5
BRENDA, the Enzyme Database: 4.2.2.5
CAS: 9047-57-8

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