KEGG   ENZYME: 4.2.3.131Help
Entry
EC 4.2.3.131                Enzyme                                 

Name
miltiradiene synthase;
SmMDS;
SmiKSL;
RoKSL
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(+)-copalyl-diphosphate diphosphate-lyase (cyclizing, miltiradiene-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate = miltiradiene + diphosphate [RN:R10585]
Reaction(KEGG)
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
miltiradiene [CPD:C20711];
diphosphate [CPD:C00013]
Comment
Isolated from the plants Rosmarinus officinalis (rosemary) and Salvia miltiorrhiza. The enzyme from the plant Selaginella moellendorffii is mutifunctional and also catalyses EC 5.5.1.12, copalyl diphosphate synthase [2].
History
EC 4.2.3.131 created 2012
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K18113  miltiradiene synthase / copalyl diphosphate synthase
Genes
SMO: SELMODRAFT_450918
Taxonomy
Reference
1  [PMID:19905026]
  Authors
Gao W, Hillwig ML, Huang L, Cui G, Wang X, Kong J, Yang B, Peters RJ
  Title
A functional genomics approach to tanshinone biosynthesis provides stereochemical insights.
  Journal
Org Lett 11:5170-3 (2009)
DOI:10.1021/ol902051v
Reference
2  [PMID:22027823]
  Authors
Sugai Y, Ueno Y, Hayashi K, Oogami S, Toyomasu T, Matsumoto S, Natsume M, Nozaki H, Kawaide H
  Title
Enzymatic (13)C labeling and multidimensional NMR analysis of miltiradiene synthesized by bifunctional diterpene cyclase in Selaginella moellendorffii.
  Journal
J Biol Chem 286:42840-7 (2011)
DOI:10.1074/jbc.M111.302703
  Sequence
Reference
3  [PMID:24569175]
  Authors
Bruckner K, Bozic D, Manzano D, Papaefthimiou D, Pateraki I, Scheler U, Ferrer A, de Vos RC, Kanellis AK, Tissier A
  Title
Characterization of two genes for the biosynthesis of abietane-type diterpenes in rosemary (Rosmarinus officinalis) glandular trichomes.
  Journal
Phytochemistry 101:52-64 (2014)
DOI:10.1016/j.phytochem.2014.01.021
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.131
IUBMB Enzyme Nomenclature: 4.2.3.131
ExPASy - ENZYME nomenclature database: 4.2.3.131
BRENDA, the Enzyme Database: 4.2.3.131

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