KEGG   ENZYME: 4.2.3.132Help
Entry
EC 4.2.3.132                Enzyme                                 

Name
neoabietadiene synthase;
AgAS;
PtTPS-LAS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(+)-copaly-diphosphate diphosphate-lyase (cyclizing, neoabietadiene-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate = neoabietadiene + diphosphate [RN:R06303]
Reaction(KEGG)
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
neoabietadiene [CPD:C11880];
diphosphate [CPD:C00013]
Comment
Isolated from Abies grandis (grand fir) [1]. This class I enzyme forms about equal proportions of abietadiene, levopimaradiene and neoabietadiene. See also EC 4.2.3.18, abieta-7,13-diene synthase and EC 4.2.3.32, levopimaradiene synthase. An X-ray study of this multifunctional enzyme showed that the class I activity is in the alpha domain, while (+)-copalyl diphosphate synthase activity (EC 5.5.1.12, a class II activity) is in the beta and gamma domains [2]. In Pinus taeda (loblolly pine) the major product is levopimaradiene, with less abietadiene and neoabietadiene [3].
History
EC 4.2.3.132 created 2012
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K12927  abietadiene/neoabietadiene/copalyl diphosphate synthase
K19571  levopimaradiene/neoabietadiene/copalyl diphosphate synthase
Reference
1  [PMID:11112547]
  Authors
Peters RJ, Flory JE, Jetter R, Ravn MM, Lee HJ, Coates RM, Croteau RB.
  Title
Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme.
  Journal
Biochemistry 39:15592-602 (2000)
DOI:10.1021/bi001997l
  Sequence
Reference
2  [PMID:22219188]
  Authors
Zhou K, Gao Y, Hoy JA, Mann FM, Honzatko RB, Peters RJ
  Title
Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis.
  Journal
J Biol Chem 287:6840-50 (2012)
DOI:10.1074/jbc.M111.337592
  Sequence
Reference
3  [PMID:16497345]
  Authors
Ro DK, Bohlmann J.
  Title
Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1).
  Journal
Phytochemistry 67:1572-8 (2006)
DOI:10.1016/j.phytochem.2006.01.011
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.132
IUBMB Enzyme Nomenclature: 4.2.3.132
ExPASy - ENZYME nomenclature database: 4.2.3.132
BRENDA, the Enzyme Database: 4.2.3.132

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