KEGG   ENZYME: 4.2.3.32Help
Entry
EC 4.2.3.32                 Enzyme                                 

Name
levopimaradiene synthase;
PtTPS-LAS;
LPS;
copalyl-diphosphate diphosphate-lyase [abieta-8(14),12-diene-forming]
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(+)-copalyl-diphosphate diphosphate-lyase [abieta-8(14),12-diene-forming]
Reaction(IUBMB)
(+)-copalyl diphosphate = abieta-8(14),12-diene + diphosphate [RN:R06302]
Reaction(KEGG)
R06302;
(other) R06304
Show
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
abieta-8(14),12-diene [CPD:C11879];
diphosphate [CPD:C00013]
Comment
In Ginkgo, the enzyme catalyses the initial cyclization step in the biosynthesis of ginkgolides, a structurally unique family of diterpenoids that are highly specific platelet-activating-factor receptor antagonists [1]. Levopimaradiene is widely distributed in higher plants. In some species the enzyme also forms abietadiene, palustradiene, and neoabietadiene [2].
History
EC 4.2.3.32 created 2008, modified 2012
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K14041  levopimaradiene/copalyl diphosphate synthase
K19571  levopimaradiene/neoabietadiene/copalyl diphosphate synthase
Reference
1  [PMID:11488601]
  Authors
Schepmann HG, Pang J, Matsuda SP.
  Title
Cloning and characterization of Ginkgo biloba levopimaradiene synthase which catalyzes the first committed step in ginkgolide biosynthesis.
  Journal
Arch Biochem Biophys 392:263-9 (2001)
DOI:10.1006/abbi.2001.2438
  Sequence
Reference
2  [PMID:16497345]
  Authors
Ro DK, Bohlmann J.
  Title
Diterpene resin acid biosynthesis in loblolly pine (Pinus taeda): functional characterization of abietadiene/levopimaradiene synthase (PtTPS-LAS) cDNA and subcellular targeting of PtTPS-LAS and abietadienol/abietadienal oxidase (PtAO, CYP720B1).
  Journal
Phytochemistry 67:1572-8 (2006)
DOI:10.1016/j.phytochem.2006.01.011
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.32
IUBMB Enzyme Nomenclature: 4.2.3.32
ExPASy - ENZYME nomenclature database: 4.2.3.32
BRENDA, the Enzyme Database: 4.2.3.32

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