KEGG   ENZYME: 4.2.3.36Help
Entry
EC 4.2.3.36                 Enzyme                                 

Name
terpentetriene synthase;
Cyc2
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
terpentedienyl-diphosphate diphosphate-lyase (terpentetriene-forming)
Reaction(IUBMB)
terpentedienyl diphosphate = terpentetriene + diphosphate [RN:R09684]
Reaction(KEGG)
Substrate
terpentedienyl diphosphate [CPD:C19815]
Product
terpentetriene [CPD:C19814];
diphosphate [CPD:C00013]
Comment
Requires Mg2+ for maximal activity but can use Mn2+, Fe2+ or Co2+ to a lesser extent [2]. Following on from EC 5.5.1.15, terpentedienyl-diphosphate synthase, this enzyme completes the transformation of geranylgeranyl diphosphate (GGDP) into terpentetriene, which is a precursor of the diterpenoid antibiotic terpentecin. Farnesyl diphosphate can also act as a substrate.
History
EC 4.2.3.36 created 2008
Pathway
ec00999  Biosynthesis of secondary metabolites - unclassified
ec01130  Biosynthesis of antibiotics
Orthology
K19834  terpentetriene synthase
Reference
1  [PMID:11567009]
  Authors
Dairi T, Hamano Y, Kuzuyama T, Itoh N, Furihata K, Seto H.
  Title
Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin.
  Journal
J Bacteriol 183:6085-94 (2001)
DOI:10.1128/JB.183.20.6085-6094.2001
  Sequence
Reference
2  [PMID:12138123]
  Authors
Hamano Y, Kuzuyama T, Itoh N, Furihata K, Seto H, Dairi T.
  Title
Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin.
  Journal
J Biol Chem 277:37098-104 (2002)
DOI:10.1074/jbc.M206382200
  Sequence
Reference
3  [PMID:12839434]
  Authors
Eguchi T, Dekishima Y, Hamano Y, Dairi T, Seto H, Kakinuma K.
  Title
A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and 1H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase.
  Journal
J Org Chem 68:5433-8 (2003)
DOI:10.1021/jo026728a
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.36
IUBMB Enzyme Nomenclature: 4.2.3.36
ExPASy - ENZYME nomenclature database: 4.2.3.36
BRENDA, the Enzyme Database: 4.2.3.36

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