KEGG   ENZYME: 4.4.1.1Help
Entry
EC 4.4.1.1                  Enzyme                                 

Name
cystathionine gamma-lyase;
homoserine deaminase;
homoserine dehydratase;
cystine desulfhydrase;
cysteine desulfhydrase;
gamma-cystathionase;
cystathionase;
homoserine deaminase-cystathionase;
gamma-CTL;
cystalysin;
cysteine lyase;
L-cystathionine cysteine-lyase (deaminating);
CGL
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
L-cystathionine cysteine-lyase (deaminating; 2-oxobutanoate-forming)
Reaction(IUBMB)
L-cystathionine + H2O = L-cysteine + 2-oxobutanoate + NH3 (overall reaction) [RN:R01001];
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate [RN:R08632];
(1b) 2-aminobut-2-enoate = 2-iminobutanoate (spontaneous);
(1c) 2-iminobutanoate + H2O = 2-oxobutanoate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
L-cystathionine [CPD:C02291];
H2O [CPD:C00001];
2-aminobut-2-enoate [CPD:C17234];
2-iminobutanoate [CPD:C20905]
Product
L-cysteine [CPD:C00097];
2-oxobutanoate [CPD:C00109];
NH3 [CPD:C00014];
2-aminobut-2-enoate [CPD:C17234];
2-iminobutanoate [CPD:C20905]
Comment
A multifunctional pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing L-cysteine and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form 2-oxobutanoate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. Also catalyses the conversion of L-homoserine to 2-oxobutanoate and ammonia, of L-cystine to thiocysteine, pyruvate and ammonia, and of L-cysteine to pyruvate, hydrogen sulfide and ammonia.
History
EC 4.4.1.1 created 1961 (EC 4.2.1.15 created 1961, incorporated 1972)
Pathway
ec00260  Glycine, serine and threonine metabolism
ec00270  Cysteine and methionine metabolism
ec00450  Selenocompound metabolism
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K01758  cystathionine gamma-lyase
K17217  cystathionine gamma-lyase / homocysteine desulfhydrase
Genes
HSA: 1491(CTH)
PTR: 456939(CTH)
PPS: 100968341(CTH)
GGO: 101127547(CTH)
PON: 100171474(CTH)
NLE: 100592397(CTH)
MCC: 696664(CTH)
MCF: 102117863(CTH) 102145842(CTH)
CSAB: 103224624(CTH)
RRO: 104658601(CTH)
RBB: 108534624(CTH)
CJC: 100401519(CTH)
SBQ: 101040933(CTH)
MMU: 107869(Cth)
RNO: 103691744 24962(Cth)
CGE: 100752127(Cth)
NGI: 103744340(Cth)
HGL: 101718068(Cth)
OCU: 100348407(CTH)
TUP: 102493127(CTH)
CFA: 479991(CTH)
AML: 100466530(CTH)
UMR: 103663016(CTH)
ORO: 101369937(CTH)
FCA: 101083282(CTH)
PTG: 102949336(CTH)
AJU: 106982700(CTH)
BTA: 539159(CTH)
BOM: 102269372(CTH)
BIU: 109556843(CTH) 109561076
PHD: 102320173(CTH)
CHX: 102172231(CTH)
OAS: 101118487(CTH)
SSC: 733654(CTH)
CFR: 102505835(CTH)
CDK: 105096636(CTH)
BACU: 103009716(CTH)
LVE: 103076358(CTH)
OOR: 101278105(CTH)
ECB: 100053614(CTH)
EPZ: 103543160(CTH)
EAI: 106847815(CTH)
MYB: 102246657(CTH)
MYD: 102766306(CTH)
HAI: 109373855(CTH)
RSS: 109460057(CTH)
PALE: 102890101(CTH)
LAV: 100675573(CTH)
TMU: 101354471
MDO: 100029728(CTH)
SHR: 100932012(CTH)
OAA: 100073790(CTH)
GGA: 424716(CTH)
MGP: 100541672(CTH)
CJO: 107317790(CTH)
APLA: 101795717(CTH)
ACYG: 106043627(CTH)
TGU: 100232398(CTH)
GFR: 102037173 102044932(CTH)
FAB: 101809409(CTH) 101816380
PHI: 102103541(CTH) 102111557
PMAJ: 107207894(CTH) 107207896
CCW: 104695486(CTH)
FPG: 101920503(CTH)
FCH: 102054097(CTH)
CLV: 102092517(CTH)
EGZ: 104123861(CTH)
AAM: 106486509(CTH)
ASN: 102369692(CTH)
AMJ: 102570419(CTH)
PSS: 102450482(CTH)
CMY: 102930648(CTH)
CPIC: 101941252(CTH)
ACS: 100555553(cth)
PBI: 103067481(CTH)
GJA: 107114344(CTH)
XLA: 108715515(cth.S) 494673(cth.L)
XTR: 394634(cth)
NPR: 108804937(CTH)
DRE: 322055(cth)
SRX: 107711102(cth) 107726514
CCAR: 109063744 109104396(cth)
IPU: 108271847(cth)
AMEX: 103034904(cth)
TRU: 101064271(cth)
LCO: 104917765(cth) 109136898
NCC: 104941401(cth)
MZE: 101468515(cth)
OLA: 101164297(cth)
XMA: 102225184(cth)
PRET: 103463490(cth)
NFU: 107392698(cth)
KMR: 108234240(cth)
CSEM: 103392476(cth)
LCF: 108881260(cth)
SDU: 111216557(cth)
HCQ: 109522055(cth)
BPEC: 110160141(cth)
MALB: 109958292(cth)
SASA: 100194964(cgl) 106573862
ELS: 105011420(cth)
SFM: 108919087(cth)
CMK: 103189141(cth)
CIN: 100184574
SPU: 762952
APLC: 110977442
DME: Dmel_CG5345(Eip55E)
DSI: Dsimw501_GD11400(Dsim_GD11400)
AAG: 5564056
AME: 412460
BIM: 100744078
BTER: 100649268
SOC: 105193104
PGC: 109855315
PCF: 106788843
MDL: 103580034
TCA: 661175
BMOR: 692801
PMAC: 106717588
PRAP: 111003837
PXY: 105396734
API: 100159197 100159560(ACYPI000433) 100168016
CLEC: 106662628
TUT: 107367720
CEL: CELE_F22B8.6(cth-1) CELE_ZK1127.10(cth-2)
CBR: CBG24746
BMY: Bm1_05320
TSP: Tsp_06337
CRG: 105327873
MYI: 110442827
OBI: 106884197
LAK: 106172713
SHX: MS3_09576
HMG: 100214967
SCE: YAL012W(CYS3)
ERC: Ecym_2629
KMX: KLMA_20354(CYS3)
NCS: NCAS_0F01520(NCAS0F01520)
NDI: NDAI_0H02400(NDAI0H02400)
TPF: TPHA_0L00570(TPHA0L00570)
TBL: TBLA_0E02240(TBLA0E02240)
TDL: TDEL_0H03530(TDEL0H03530)
KAF: KAFR_0K00760(KAFR0K00760)
PIC: PICST_77247(CYS3)
SPAA: SPAPADRAFT_59236(CYS3)
CAL: CAALFM_CR08340WA(CYS3)
CAUR: QG37_02634
SLB: AWJ20_3470(CYS3)
NCR: NCU09230(cys-16)
NTE: NEUTE1DRAFT126996(NEUTE1DRAFT_126996)
MGR: MGG_10380
SSCK: SPSK_00793
MAW: MAC_04206
MAJ: MAA_03905
CMT: CCM_07010
BFU: BCIN_02g03490(Bccys3)
MBE: MBM_02250
ANI: AN1446.2
ANG: ANI_1_1172144(An16g08720)
ABE: ARB_06473
TVE: TRV_00587
PTE: PTT_17380
CNE: CNN01730
CNB: CNBN1680
ABP: AGABI1DRAFT62583(AGABI1DRAFT_62583)
ABV: AGABI2DRAFT209095(AGABI2DRAFT_209095)
DDI: DDB_G0269122(cysA)
DFA: DFA_09926(cysA)
ENC: ECL_02361
EEC: EcWSU1_01869(metC)
ECLX: LI66_09400
ECLY: LI62_10170
ESA: ESA_02766
CSK: ES15_2856
CTU: CTU_11060(metC)
KPN: KPN_03410(metB)
KPU: KP1_4685(metB)
KPP: A79E_0712
KPT: VK055_4065(metC3)
KPE: KPK_0708
KPJ: N559_0771
KPX: PMK1_00885(metC_1)
KPNU: LI86_03735
KPNK: BN49_0694
KVA: Kvar_0681
KOX: KOX_02800
KOE: A225_5009
EAE: EAE_18840
EAR: CCG30115
CKO: CKO_01707
YEN: YE1469(metB)
YEY: Y11_03501
YEW: CH47_887(metB)
YET: CH48_178
YEE: YE5303_13191(metB)
YAL: AT01_1035 AT01_3688(metC)
YFR: AW19_1753(metB)
YIN: CH53_218 CH53_471(metB)
YKR: CH54_4033
YRO: CH64_1105
YRU: BD65_3184
SPE: Spro_1626
SRL: SOD_c14970(metC1)
SPLY: Q5A_008095(metC_1)
SMAF: D781_1540
SMAR: SM39_1069
SERF: L085_20425
PLU: plu0523
PAY: PAU_00517
XBO: XBJ1_3500(metB)
XBV: XBW1_3931(metC)
XNE: XNC1_1035(metB)
XNM: XNC2_1019(metB)
XDO: XDD1_0530 XDD1_3039(metC)
XPO: XPG1_0660(metC) XPG1_3366(metC)
PRG: RB151_031470(metC_3)
MMK: MU9_384
ETR: ETAE_1929(metB)
ETD: ETAF_1741
ETE: ETEE_4005
XCC: XCC0598(metB)
XCB: XC_3635
XCP: XCR_0751
XCV: XCV3725
XAX: XACM_3502
XAC: XAC3602(metB)
XCI: XCAW_04304(metC)
XOO: XOO0778(metB)
XOM: XOO0708(XOO0708)
XOP: PXO_03157(metB)
XOR: XOC_3870(metB)
XAL: XALC_2712
XPH: XppCFBP6546_16310(XppCFBP6546P_16310)
SML: Smlt0617(mtcC)
SMT: Smal_0489
SMZ: SMD_0528(metC)
SACZ: AOT14_06070(cys1_1)
PSUW: WQ53_11135
PSD: DSC_02420
LAQ: GLA29479_2082(metC)
LCP: LC55x_4385(cysA)
LGU: LG3211_4190(cysA)
LEZ: GLE_4248(mdeA)
LEM: LEN_0953(metB)
DKO: I596_3785
PAE: PA0400
PAEV: N297_411(metC)
PAEI: N296_411(metC)
PAEP: PA1S_02030
PAEM: U769_02065
PAEL: T223_02025
PAEG: AI22_01870
PAEC: M802_410(metC)
PAEO: M801_411(metC)
PCQ: PcP3B5_05410(metC)
PPU: PP_4594
PPF: Pput_1294
PPT: PPS_3955
PPI: YSA_07716
PPX: T1E_3138
PPUH: B479_19660
PPUT: L483_25075
PPUN: PP4_12060
PPUD: DW66_4393
PMON: X969_19310
PMOT: X970_18945
PFC: PflA506_2875(metC)
PFB: VO64_0544
PMAN: OU5_5249
PKC: PKB_0419(metC)
PSEM: TO66_08615
PSEC: CCOS191_3987(metC)
PANR: A7J50_3130
PSET: THL1_260
PSIL: PMA3_16730
ILO: IL0219(metB)
GNI: GNIT_2090
LHA: LHA_2062(metC)
LOK: Loa_01894
LCD: clem_04985(metC)
LLG: 44548918_00921(metC)
KKO: Kkor_1765
KGE: TQ33_0690
CVI: CV_3394 CV_4049(metB)
POL: Bpro_2831
JAG: GJA_2052(metC)
CFU: CFU_1538(metB)
CARE: LT85_3219
CPRA: CPter91_2009(metC)
RGE: RGE_20070(metB)
RDP: RD2015_66
DSU: Dsui_2042
ADE: Adeh_3707
MXA: MXAN_2035
CCX: COCOR_06056(metB1)
CCRO: CMC5_025910(metB) CMC5_050890(metB)
HOH: Hoch_6707
BBA: Bd3795(metB)
BBAT: Bdt_3674(metB)
BBW: BDW_13960
BBAC: EP01_11480
BEX: A11Q_2498
BMX: BMS_3012(metC)
AMIH: CO731_00551(metC_1) CO731_02780(mccB)
OAH: DR92_3870(metC)
RPA: RPA0371(metB) RPA2357
VGO: GJW-30_1_02183(metC_1)
BVR: BVIR_362
RBM: TEF_12275
CAK: Caul_4904
PZU: PHZ_c0072
SIL: SPO3344
PSF: PSE_0057(metB)
HNE: HNE_1212
RRU: Rru_A0786
RRF: F11_04030
RCE: RC1_1986
BSU: BSU27250(yrhB)
BSR: I33_2767(yrhB)
BSL: A7A1_0367
BSH: BSU6051_27250(mccB)
BSUT: BSUB_02907(mccB)
BSUL: BSUA_02907(mccB)
BSUS: Q433_14680
BSS: BSUW23_13180(mccB)
BST: GYO_2965(yrhB)
BSO: BSNT_09119(yrhB)
BSQ: B657_27250(mccB)
BSX: C663_2562(mccB)
BLI: BL02018(yrhB)
BLD: BLi02853(mccB)
BLH: BaLi_c29540(mccB)
BAY: RBAM_024350(yrhB)
BAQ: BACAU_2449(yrhB)
BYA: BANAU_2593(yrhB)
BAMP: B938_12585
BAML: BAM5036_2375(mccB)
BAMA: RBAU_2572(mccB)
BAMN: BASU_2378(mccB)
BAMB: BAPNAU_1127(yrhB)
BAMT: AJ82_13760
BAMY: V529_27190(yrhB)
BMP: NG74_02557(mccB)
BAO: BAMF_2533(mccB)
BAZ: BAMTA208_13370(mccB)
BQL: LL3_02811(mccB)
BXH: BAXH7_02735(yrhB)
BAMI: KSO_007200
BAMC: U471_25270
BAMF: U722_13215
BAN: BA_4600
BAR: GBAA_4600
BAT: BAS4268
BAI: BAA_4620
BANT: A16_45980
BANR: A16R_60345
BANS: BAPAT_4415
BANV: DJ46_3277(mccB)
BCE: BC4366
BCA: BCE_4454(metC)
BCZ: BCE33L4116(metC)
BCQ: BCQ_4156(metC)
BCX: BCA_4484
BAL: BACI_c43580(metC2)
BNC: BCN_4282
BCF: bcf_21745
BCER: BCK_13335
BTK: BT9727_4105(metC)
BTL: BALH_3957(metC)
BTT: HD73_4680
BTHI: BTK_23050
BTM: MC28_3663
BTG: BTB_c45120(mccB)
BTI: BTG_27215
BTW: BF38_126(mccB)
BWW: bwei_0567(mccB)
BMYO: BG05_1654
BMYC: DJ92_1469(mccB)
BCL: ABC1945
BPU: BPUM_2360
BPUM: BW16_12815
BPUS: UP12_11885
BMQ: BMQ_4594(mccB)
BMD: BMD_4580(mccB)
BAG: Bcoa_1961
BCOA: BF29_1572(mccB)
BJS: MY9_2706
BMET: BMMGA3_12170(mccB)
BACW: QR42_11960
BACP: SB24_16520
BACB: OY17_15455
BACY: QF06_11815
BACL: BS34A_29730(yrhB)
BALM: BsLM_2694
BEO: BEH_16500
BGY: BGLY_3172(mccB)
OIH: OB1109(metC)
GKA: GK2540
GTN: GTNG_2473
GGH: GHH_c26140(mcc)
GEA: GARCT_02594(mccB)
AFL: Aflv_0761
AAMY: GFC30_2789
HHD: HBHAL_2023(mccB)
SAU: SA0419(metB)
SAV: SAV0460(yrhB)
SAW: SAHV_0458(yrhB)
SAM: MW0415(metB)
SAS: SAS0418
SAR: SAR0460
SAC: SACOL0503
SAA: SAUSA300_0434(metB)
SAE: NWMN_0425(metB)
SAD: SAAV_0403
SUE: SAOV_0478
SUJ: SAA6159_00410(metB)
SUK: SAA6008_00464(metB)
SUC: ECTR2_394
SUZ: MS7_0434(mccB)
SUG: SAPIG0526
SAUA: SAAG_00915
SAUS: SA40_0399
SAUU: SA957_0414
SAUG: SA268_0412
SAUT: SAI1T1_2003360(yrhB)
SAUJ: SAI2T2_1003360(yrhB)
SAUK: SAI3T3_1003360(yrhB)
SAUQ: SAI4T8_1003360(yrhB)
SAUV: SAI7S6_1003360(metB)
SAUW: SAI5S5_1003350(metB)
SAUX: SAI6T6_1003360(metB)
SAUY: SAI8T7_1003360(metB)
SAUF: X998_0487
SAB: SAB0410
SUY: SA2981_0436(metB)
SAUB: C248_0510
SAUM: BN843_4430
SAUC: CA347_454(mccB)
SAUR: SABB_02175(mccB)
SAUI: AZ30_02230
SAUD: CH52_03550
SAMS: NI36_02155
SEP: SE2323
SER: SERP0095
SEPP: SEB_02318
SEPS: DP17_1515
SHA: SH2548(metB)
SHH: ShL2_02333(metB_2)
SSP: SSP0253
SCA: SCA_0087(metC)
SDT: SPSE_2355(metB2)
SPAS: STP1_1535
SXO: SXYL_00285(metB)
SHU: SHYC_11450(mccB)
SCAP: AYP1020_2183(mccB)
SSCH: LH95_11030
SSCZ: RN70_11795
SAGQ: EP23_10730
MCL: MCCL_1790
MCAK: MCCS_23060(mccB)
ESI: Exig_2343
EAN: Eab7_2188
BBE: BBR47_47850(mccB)
PPY: PPE_02107
PPM: PPSC2_11035(yrhB)
PPO: PPM_2116(yrhB)
PPOL: X809_11380
PPOY: RE92_01315
ASOC: CB4_01041(mccB_2)
JEO: JMA_30770
CRN: CAR_c05790(yrhB)
CAE: SMB_G0947(mccB)
ROB: CK5_00560
MPA: MAP_3053c
MAO: MAP4_0747
MAVI: RC58_03650
MAVU: RE97_03655
MAV: MAV_3867
MIT: OCO_36860
MIA: OCU_36940
MID: MIP_05582
MYO: OEM_37520
MIR: OCQ_38070
MMC: Mmcs_1928
MKM: Mkms_1974
MJL: Mjls_1908
MMM: W7S_18450
MVA: Mvan_2142
MGI: Mflv_4220
MPHL: MPHLCCUG_02066(mccB)
MVQ: MYVA_2031
MTHN: 4412656_01566(mdeA_2)
MAB: MAB_3287c
MABB: MASS_3232
MCHE: BB28_16585
MSTE: MSTE_03264
RER: RER_24150
REY: O5Y_11340
ROP: ROP_65570
RRT: 4535765_03144(mdeA_1)
SRT: Srot_2032
SCO: SCO3920(cysA)
SALB: XNR_2948
SMA: SAVERM_4273(cysA)
SGR: SGR_3660
SGB: WQO_17420
SCB: SCAB_46251(cysA)
SCT: SCAT_3174(cysA)
SFA: Sfla_3140
SBH: SBI_05334
SHY: SHJG_5150
SDV: BN159_4567(cysA1)
SALS: SLNWT_3693
STRP: F750_3616
SFI: SFUL_3600
SALU: DC74_4260
SALL: SAZ_22690
SGU: SGLAU_16825(cysA)
STRE: GZL_04627
SLD: T261_4090
STRM: M444_17605
SAMB: SAM23877_3735(cysA)
SPRI: SPRI_3832
SRW: TUE45_03787(metB_2)
SLE: sle_36370(sle_36370)
SRN: A4G23_02776(metC)
STRD: NI25_19410
SMAL: SMALA_3849
SLX: SLAV_19030(metB2)
SFK: KY5_4018
KFL: Kfla_4026
ACE: Acel_1425
GOB: Gobs_4046
BSD: BLASA_3826(cysA2)
MMAR: MODMU_4454
SEN: SACE_6136
PDX: Psed_1840
PSEA: WY02_10115
PSEE: FRP1_04130
PSEH: XF36_18705
AMI: Amir_5997
SESP: BN6_71800(cysA1)
KAL: KALB_7393
ACTI: UA75_26390
ACAD: UA74_25805
AHG: AHOG_23705(metC)
SAQ: Sare_1158
MIL: ML5_1584
ASE: ACPL_7337(cysA)
ACTN: L083_6927
AFS: AFR_36645
ACTS: ACWT_7207
BLO: BL1559
CWO: Cwoe_1684
GLJ: GKIL_3015
RBA: RB6443(metB)
PIR: VN12_17070(metC_2)
TTF: THTE_3938
GES: VT84_31095(metC)
SACI: Sinac_7194
PBOR: BSF38_05619(metC)
ABAC: LuPra_04062(metC)
GAU: GAU_0234 GAU_2431(metC)
GBA: J421_3896
PHE: Phep_2908
SLI: Slin_5637
CHE: CAHE_0218(metC)
CEC: CE557_365
IAL: IALB_2140
TPT: Tpet_1501
TLE: Tlet_0460
METH: MBMB1_1133(metC)
TAC: Ta0080
PYN: PNA2_1581
TLT: OCC_05686
THS: TES1_1396
PPAC: PAP_08750
MBN: Mboo_1991
MPL: Mpal_1156
MEZ: Mtc_1605
HAL: VNG_0796G(cgs)
HSL: OE_2173F(metB2)
HHI: HAH_2847(metB)
HVO: HVO_2946(metB2)
HME: HFX_2939(metB2)
HTU: Htur_4640
NMG: Nmag_1099(metB)
APE: APE_1226(metC)
ACJ: ACAM_0776
 » show all
Taxonomy
Reference
1
  Authors
Braunstein, A.E. and Azarkh, R.M.
  Title
[Participation of vitamin B6 in enzymic formation of hydrogen sulfide from L-cysteine.].
  Journal
Dokl Akad Nauk SSSR 71:93-96 (1950)
Reference
2  [PMID:12998462]
  Authors
BRAUNSHTEIN AE, AZARKH RM
  Title
[Role of pyridoxal phosphate in anaerobic deamination of homoserine and serine].
  Journal
Dokl Akad Nauk SSSR 85:385-8 (1952)
Reference
3  [PMID:14209951]
  Authors
FLAVIN M, SEGAL A.
  Title
PURIFICATION AND PROPERTIES OF THE CYSTATHIONINE GAMMA-CLEAVAGE ENZYME OF NEUROSPORA.
  Journal
J Biol Chem 239:2220-7 (1964)
Reference
4  [PMID:13641250]
  Authors
MATSUO Y, GREENBERG DM.
  Title
A crystalline enzyme that cleaves homoserine and cystathionine. III. Coenzyme resolution, activators, and inhibitors.
  Journal
J Biol Chem 234:507-15 (1959)
Reference
5  [PMID:13641251]
  Authors
MATSUO Y, GREENBERG DM.
  Title
A crystalline enzyme that cleaves homoserine and cystathionine. IV. Mechanism of action, reversibility, and substrate specificity.
  Journal
J Biol Chem 234:516-9 (1959)
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.1
IUBMB Enzyme Nomenclature: 4.4.1.1
ExPASy - ENZYME nomenclature database: 4.4.1.1
BRENDA, the Enzyme Database: 4.4.1.1
CAS: 9012-96-8

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