KEGG   ENZYME: 4.99.1.5Help
Entry
EC 4.99.1.5                 Enzyme                                 

Name
aliphatic aldoxime dehydratase;
OxdA;
aliphatic aldoxime hydro-lyase
Class
Lyases;
Other lyases;
Sole sub-subclass for lyases that do not belong in the other subclasses
BRITE hierarchy
Sysname
aliphatic aldoxime hydro-lyase (aliphatic-nitrile-forming)
Reaction(IUBMB)
an aliphatic aldoxime = an aliphatic nitrile + H2O [RN:R02827]
Reaction(KEGG)
Substrate
aliphatic aldoxime [CPD:C02658]
Product
aliphatic nitrile [CPD:C16072];
H2O [CPD:C00001]
Comment
The enzyme from Pseudomonas chlororaphis contains Ca2+ and protoheme IX, the iron of which must be in the form Fe(II) for activity. The enzyme exhibits a strong preference for aliphatic aldoximes, such as butyraldoxime and acetaldoxime, over aromatic aldoximes, such as pyridine-2-aldoxime, which is a poor substrate. No activity was found with the aromatic aldoximes benzaldoxime and pyridine-4-aldoxime.
History
EC 4.99.1.5 created 2004
Orthology
K13028  aldoxime dehydratase
Genes
GQU: AWC35_07535
KRD: A3780_03605
SRY: M621_16765
ROX: BV494_24265
EBI: EbC_pEb17201260
PVA: Pvag_pPag20172(oxd)
PAO: Pat9b_4157
PPF: Pput_2724
PPB: PPUBIRD1_2719
PPI: YSA_10164
PSB: Psyr_0006
PFS: PFLU_3206
PSET: THL1_3212
ACI: ACIAD1620(oxd)
SWD: Swoo_0211
GAI: IMCC3135_05130(oxd)
BCN: Bcen_4080
BAM: Bamb_6546
BCON: NL30_05545
BSEM: WJ12_26915
BMEC: WJ16_18455
BHZ: ACR54_04419(oxd)
AAA: Acav_2428
HSE: Hsero_0929(oxdA)
HRB: Hrubri_2880(oxdA) Hrubri_3579(oxdA)
SFH: SFHH103_06512(oxd)
BBT: BBta_4765
BRS: S23_30650
BRAD: BF49_6425
XAU: Xaut_1560
HMC: HYPMC_0479(Adase)
SWI: Swit_0988
SPHD: HY78_21570
SPHR: BSY17_2187(oxd)
GOH: B932_2217
RER: RER_59190
REY: O5Y_28495
SALB: XNR_1370
ARR: ARUE_c20310(oxd)
AAU: AAur_1899(oxdA)
PSIM: KR76_24340
PSEE: FRP1_16645
PSEH: XF36_16225
AMI: Amir_0081
 » show all
Taxonomy
Reference
1  [PMID:12773527]
  Authors
Oinuma K, Hashimoto Y, Konishi K, Goda M, Noguchi T, Higashibata H, Kobayashi M.
  Title
Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chlororaphis B23. Sequencing, gene expression, purification, and characterization.
  Journal
J Biol Chem 278:29600-8 (2003)
DOI:10.1074/jbc.M211832200
  Sequence
Reference
2  [PMID:14556637]
  Authors
Xie SX, Kato Y, Komeda H, Yoshida S, Asano Y.
  Title
A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4.
  Journal
Biochemistry 42:12056-66 (2003)
DOI:10.1021/bi035092u
  Sequence
Reference
3  [PMID:16233624]
  Authors
Kato Y, Yoshida S, Xie SX, Asano Y.
  Title
Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771.
  Journal
J Biosci Bioeng 97:250-9 (2004)
DOI:10.1016/S1389-1723(04)70200-5
  Sequence
[rer:RER_59190]
Other DBs
ExplorEnz - The Enzyme Database: 4.99.1.5
IUBMB Enzyme Nomenclature: 4.99.1.5
ExPASy - ENZYME nomenclature database: 4.99.1.5
BRENDA, the Enzyme Database: 4.99.1.5

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