KEGG   ENZYME: 5.1.3.23Help
Entry
EC 5.1.3.23                 Enzyme                                 

Name
UDP-2,3-diacetamido-2,3-dideoxyglucuronic acid 2-epimerase;
UDP-GlcNAc3NAcA 2-epimerase;
UDP-alpha-D-GlcNAc3NAcA 2-epimerase;
2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerase;
WbpI;
WlbD
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
BRITE hierarchy
Sysname
2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate 2-epimerase
Reaction(IUBMB)
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate = UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate [RN:R09600]
Reaction(KEGG)
Substrate
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronate [CPD:C19725]
Product
UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronate [CPD:C19745]
Comment
This enzyme participates in the biosynthetic pathway for UDP-alpha-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-mannuronic acid), an important precursor of the B-band lipopolysaccharide of Pseudomonas aeroginosa serotype O5 and of the band-A trisaccharide of Bordetella pertussis, both important respiratory pathogens [1]. The enzyme is highly specific as UDP-alpha-D-GlcNAc, UDP-alpha-D-GlcNAcA (UDP-2-acetamido-2-deoxy-alpha-D-glucuronic acid) and UDP-alpha-D-GlcNAc3NAc (UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucose) cannot act as substrates [1].
History
EC 5.1.3.23 created 2007
Pathway
ec00520  Amino sugar and nucleotide sugar metabolism
Orthology
K13019  UDP-GlcNAc3NAcA epimerase
Genes
PLU: plu4804(wblH)
PLUM: A4R40_23895
PAY: PAU_04319(wblH)
PTT: VY86_07555
PAE: PA3148(wbpI)
PAEV: N297_3260
PAEI: N296_3260
PAEC: M802_3258
PAEO: M801_3125
PDR: H681_23245
PSES: PSCI_2869
SSE: Ssed_2967
AAUS: EP12_05170
TNI: TVNIR_0699(wecB_[C])
LHK: LHK_02802
PPNO: DA70_17285
BPE: BP0090(bplD)
BPC: BPTD_0086(bplD)
BPER: BN118_0159(wlbD)
BPET: B1917_0093(bplD)
BPEU: Q425_2030(bplD)
BPA: BPP0152(bplD)
BPAR: BN117_0150(wlbD)
BBR: BB0152(bplD)
BBM: BN115_0141(wlbD)
BBH: BN112_3259(wlbD)
BBX: BBS798_0151(bplD)
BPT: Bpet4819(wlbD)
BHO: D560_1331
BHM: D558_1320
BHZ: ACR54_00107(wbpI)
BTRM: SAMEA390648701339(wlbD)
TEA: KUI_0418
TEG: KUK_0136
AAV: Aave_0953
HMS: HMU06640
WSU: WS2189(WBPI)
ABU: Abu_0692
GLO: Glov_1505
DSF: UWK_02413
DOL: Dole_1828
RBS: RHODOSMS8_00383(wbpI)
SPHU: SPPYR_1658(wbpI)
MAI: MICA_1024
MAN: A11S_979
GKA: GK3162
GGH: GHH_c32370(mnaA)
AAMY: GFC30_485
CBO: CBO3100
CBA: CLB_3129
CBH: CLC_3002
CBY: CLM_3506
CBL: CLK_2494
CBK: CLL_A3182
CBB: CLD_1440
CBI: CLJ_B3366
CBT: CLH_2927
CBF: CLI_3159
CBM: CBF_3148
CBE: Cbei_4627
CBZ: Cbs_4627
CBEI: LF65_05170
CKL: CKL_3166
CKR: CKR_2804
CSQ: CSCA_4791
CTYK: CTK_C08720
CCEL: CCDG5_0710
ELM: ELI_2561
SAY: TPY_3671(wecB)
TTE: TTE0661(WecB2)
HHG: XM38_017570(wbpI)
GLJ: GKIL_0481(wecB)
CALH: IJ00_16800
RCA: Rcas_0784
CAG: Cagg_1972
TTH: TT_C0285
PUV: PUV_00130
TPED: TPE_0207
TLI: Tlie_1813
BACC: BRDCF_p1451(wecB)
TFO: BFO_2566
DORI: FH5T_09075
CPI: Cpin_4757
ZGA: ZOBELLIA_1741(wecB)
SPON: HME9304_01541(wbpI)
MRO: MROS_2673
DTU: Dtur_0574
NDV: NDEV_0205(wecB2)
 » show all
Taxonomy
Reference
1  [PMID:17346239]
  Authors
Westman EL, McNally DJ, Rejzek M, Miller WL, Kannathasan VS, Preston A, Maskell DJ, Field RA, Brisson JR, Lam JS.
  Title
Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerases from respiratory pathogens.
  Journal
Biochem J 405:123-30 (2007)
DOI:10.1042/BJ20070017
  Sequence
[pae:PA3148] [bpe:BP0090]
Reference
2
  Authors
Westman, E.L., McNally, D.J., Rejzek, M., Miller, W.L., Kannathasan, V.S., Preston, A., Maskell, D.J., Field, R.A., Brisson, J.R. and Lam, J.S.
  Title
Erratum report: Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-alpha-D-glucuronic acid 2-epimerases from respiratory pathogens.
  Journal
Biochem J 405:625 (2007)
Reference
3  [PMID:11526328]
  Authors
Sri Kannathasan V, Staines AG, Dong CJ, Field RA, Preston AG, Maskell DJ, Naismith JH.
  Title
Overexpression, purification, crystallization and data collection on the Bordetella pertussis wlbD gene product, a putative UDP-GlcNAc 2'-epimerase.
  Journal
Acta Crystallogr D Biol Crystallogr 57:1310-2 (2001)
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.23
IUBMB Enzyme Nomenclature: 5.1.3.23
ExPASy - ENZYME nomenclature database: 5.1.3.23
BRENDA, the Enzyme Database: 5.1.3.23

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