KEGG   ENZYME: 5.3.1.4Help
Entry
EC 5.3.1.4                  Enzyme                                 

Name
L-arabinose isomerase;
L-arabinose ketol-isomerase;
araA (gene name)
Class
Isomerases;
Intramolecular oxidoreductases;
Interconverting aldoses and ketoses, and related compounds
BRITE hierarchy
Sysname
L-arabinose aldose-ketose-isomerase
Reaction(IUBMB)
L-arabinose = L-ribulose [RN:R01761]
Reaction(KEGG)
Substrate
L-arabinose [CPD:C00259]
Product
L-ribulose [CPD:C00508]
Comment
Requires a divalent metal ion (the enzyme from the bacterium Escherichia coli prefers Mn2+) [2]. The enzyme binds the closed form of the sugar and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism [5]. The enzyme can also convert D-galactose to D-tagatose with lower efficiency [4].
History
EC 5.3.1.4 created 1961
Pathway
ec00040  Pentose and glucuronate interconversions
ec01100  Metabolic pathways
Orthology
K01804  L-arabinose isomerase
Genes
ECO: b0062(araA)
ECJ: JW0061(araA)
EBW: BWG_0058(araA)
ECOK: ECMDS42_0055(araA)
ECE: Z0070(araA)
ECS: ECs0066
ECF: ECH74115_0067(araA)
ETW: ECSP_0066(araA)
ELX: CDCO157_0065
EOJ: ECO26_0064(araA)
EOI: ECO111_0064(araA)
EOH: ECO103_0063(araA)
ECOO: ECRM13514_0064(araA)
ECOH: ECRM13516_0066(araA)
ECG: E2348C_0063(araA)
EOK: G2583_0065(araA)
ESO: O3O_04140
ESM: O3M_21145
ESL: O3K_21245
ECW: EcE24377A_0064(araA)
ELH: ETEC_0061
EUN: UMNK88_60
ECC: c0074(araA)
ECP: ECP_0063
ECI: UTI89_C0067(araA)
ECV: APECO1_1922(araA)
ECX: EcHS_A0066(araA)
ECM: EcSMS35_0064(araA)
ECY: ECSE_0062
ECR: ECIAI1_0062(araA)
ECQ: ECED1_0061(araA)
ECK: EC55989_0060(araA)
ECT: ECIAI39_0065(araA)
EOC: CE10_0063(araA)
EUM: ECUMN_0063(araA)
ECZ: ECS88_0065(araA)
ELO: EC042_0065(araA)
ESE: ECSF_0067
EBR: ECB_00064(araA)
EBD: ECBD_3555
EKF: KO11_00300(araA)
EAB: ECABU_c00680(araA)
EDJ: ECDH1ME8569_0061(araA)
EIH: ECOK1_0061(araA)
ENA: ECNA114_0050(araA)
ELW: ECW_m0060(araA)
ELL: WFL_00300(araA)
ELC: i14_0064(araA)
ELD: i02_0064(araA)
ELP: P12B_c0055(araA)
EBL: ECD_00064(araA)
EBE: B21_00063(araA)
ELF: LF82_0105(araA)
ECOI: ECOPMV1_00063(araA)
ECOJ: P423_00310
ECOS: EC958_0196(araA)
EFE: EFER_0071(araA)
EAL: EAKF1_ch1359(araA)
STY: STY0119(araA)
STT: t0106(araA)
STM: STM0102(araA)
SEO: STM14_0121(araA)
SEY: SL1344_0102(araA)
SEJ: STMUK_0103(araA)
SEB: STM474_0105(araA)
SEF: UMN798_0111(araA)
SENR: STMDT2_01041(araA)
SEND: DT104_01071(araA)
SENI: CY43_00500
SPT: SPA0104(araA)
SEK: SSPA0100
SEC: SCH_0096(araA)
SEH: SeHA_C0108(araA)
SHB: SU5_0735
SEE: SNSL254_A0109(araA)
SEW: SeSA_A0113(araA)
SEA: SeAg_B0113(araA)
SENS: Q786_00510
SED: SeD_A0106(araA)
SEG: SG0103(araA)
SEL: SPUL_0107(araA)
SEGA: SPUCDC_0107(araA)
SET: SEN0103(araA)
SENA: AU38_00505
SENO: AU37_00505
SENV: AU39_00505
SENQ: AU40_00575
SENL: IY59_00525
SEEP: I137_00475
SENB: BN855_1050(araA)
SENE: IA1_00510
SBG: SBG_0088(araA)
SBZ: A464_93
SFX: S0059(araA)
SFV: SFV_0054(araA)
SFE: SFxv_0059(araA)
SFN: SFy_0072
SFS: SFyv_0075
SFT: NCTC1_00057(araA)
SSN: SSON_0068(araA)
SBO: SBO_0049(araA)
SBC: SbBS512_E0054(araA)
SDY: SDY_0089(araA)
ENC: ECL_00858
ECLO: ENC_45750
EEC: EcWSU1_00675(araA)
ECLX: LI66_03545
ECLY: LI62_04035
ECLZ: LI64_03605
ENF: AKI40_4222(araA)
ESA: ESA_03277
CSK: ES15_3260
CTU: CTU_06940(araA)
KPN: KPN_00061(araA)
KPU: KP1_0874(araA)
KPP: A79E_4240
KPE: KPK_4681(araA)
KPR: KPR_0985(araA)
KPJ: N559_4370
KPX: PMK1_02369(araA)
KPNU: LI86_22450
KPNK: BN49_4279(araA)
KVA: Kvar_4322
KOX: KOX_10760
KOE: A225_0861
EAE: EAE_11090
EAR: CCG31768
CKO: CKO_03320
CRO: ROD_00691(araA)
CAMA: F384_00320
EBT: EBL_c32910(araA)
EBF: D782_3807
PSTS: E05_13100 E05_13110(araA)
YPE: YPO2253(araA)
YPK: y2094(araA)
YPH: YPC_1884(araA)
YPA: YPA_1613
YPN: YPN_1722
YPM: YP_2049(araA)
YPG: YpAngola_A2259(araA)
YPZ: YPZ3_1605(araA)
YPD: YPD4_1979(araA)
YPX: YPD8_1370
YPW: CH59_3868
YPJ: CH55_343
YPV: BZ15_1288
YPL: CH46_2861
YPS: YPTB2172(araA)
YPO: BZ17_290
YPI: YpsIP31758_1887(araA)
YPY: YPK_2001
YPB: YPTS_2242
YPQ: DJ40_132(araA)
YPU: BZ21_1453
YPR: BZ20_4077
YPC: BZ23_1738
YPF: BZ19_1530
YEN: YE2001(araA)
YEY: Y11_10121
YEW: CH47_1437(araA)
YET: CH48_3848(araA)
YEE: YE5303_18501(araA)
YAL: AT01_1158 AT01_453(araA)
YFR: AW19_1205(araA)
YIN: CH53_3943(araA)
YKR: CH54_457
YRO: CH64_547(araA)
SPE: Spro_2243
SRL: SOD_c20690(araA)
SPLY: Q5A_011505(araA)
SMAF: D781_2389
RAA: Q7S_10825
ECA: ECA2275(araA)
PATR: EV46_10930
PATO: GZ59_22870(araA)
PCT: PC1_2027
SOD: Sant_2021(araA)
DDD: Dda3937_00597(araA)
DDQ: DDI_1943
ETA: ETA_17720(araA)
EPY: EpC_18610(araA)
EPR: EPYR_02009(araA)
EAM: EAMY_1725(araA)
EAY: EAM_1696(araA)
EBI: EbC_19610(araA)
ERJ: EJP617_28600(araA)
EGE: EM595_1730(araA)
PAM: PANA_1823(araA)
PLF: PANA5342_2379(araA)
PAJ: PAJ_1159(araA)
PVA: Pvag_1249(araA)
PSTW: DSJ_13470
ETE: ETEE_3403(araA)
LRI: NCTC12151_02342(araA)
MSU: MS0058(araA)
MHAE: F382_07940
MHAM: J450_07150
MHAO: J451_08030
MHAL: N220_00125
MVE: X875_250
ASU: Asuc_0494
VPA: VPA1676
VAN: VAA_00565
VAU: VANGNB10_cI2663c(araA)
CJA: CJA_3015
SDE: Sde_0774
AHA: AHA_1900(araA)
ASA: ASA_2399(araA)
AMED: B224_1767
TAU: Tola_2162
LCH: Lcho_3203
SUR: STAUR_1920(araA)
SCL: sce3310(araA)
SME: SM_b21420
SMER: DU99_27300
RLE: RL0632(araA)
RLG: Rleg_6090
MMED: Mame_01082(araA)
JAN: Jann_3096
BSU: BSU28800(araA)
BSR: I33_2936(araA)
BSL: A7A1_3167
BSH: BSU6051_28800(araA)
BSUT: BSUB_03068(araA)
BSUL: BSUA_03068(araA)
BSUS: Q433_15580
BSS: BSUW23_13995(araA)
BST: GYO_3130(araA)
BSO: BSNT_09300(araA)
BSQ: B657_28800(araA)
BSX: C663_2725(araA)
BLI: BL00352(araA) BL01182
BLD: BLi02064(araA1) BLi03028(araA2)
BLH: BaLi_c21290(araA1) BaLi_c31090(araA2)
BAY: RBAM_025860(araA)
BAQ: BACAU_2603(araA)
BYA: BANAU_2801(araA)
BAMP: B938_13365
BAML: BAM5036_2526(araA)
BAMA: RBAU_2723(araA)
BAMN: BASU_2529(araA)
BAMB: BAPNAU_0967(araA)
BAMT: AJ82_14600
BAMY: V529_28730(araA)
BMP: NG74_02726(araA)
BAO: BAMF_2685(araA)
BAZ: BAMTA208_14150(araA)
BQL: LL3_02969(araA)
BXH: BAXH7_02898(araA)
BQY: MUS_3155(araA)
BAMI: KSO_006120
BAMC: U471_26860
BAMF: U722_14020
BHA: BH1873(araA)
BCL: ABC0408(araA)
BPU: BPUM_2327
BPUM: BW16_12610
BPUS: UP12_11750
BMQ: BMQ_3540(araA)
BMD: BMD_3530(araA)
BMH: BMWSH_1638(araA)
BMEG: BG04_415
BCK: BCO26_2127(araA)
BJS: MY9_2872
BACW: QR42_11700
BACP: SB24_15740
BACB: OY17_16235
BACY: QF06_12605
BACL: BS34A_31420(araA)
BALM: BsLM_2860
BEO: BEH_15445
BGY: BGLY_2278(araA2) BGLY_3416(araA1)
BBEV: BBEV_3000(araA)
OIH: OB2797
GKA: GK1904(araA)
GTN: GTNG_1795(araA)
GGH: GHH_c19590(araA)
AFL: Aflv_0531(araA)
ANM: GFC28_955(araA)
ANL: GFC29_671(araA)
AXL: AXY_21510(araA)
BSE: Bsel_0343
SXO: SXYL_00125(araA)
PPY: PPE_04844
PPM: PPSC2_25175(araA)
PPO: PPM_5001(araA)
PPOL: X809_41735
PPQ: PPSQR21_051010(araA)
PPOY: RE92_12085
PMS: KNP414_02519(araA)
PMW: B2K_14070
PRI: PRIO_6705(araA2)
PSWU: SY83_19980
AAC: Aaci_2897
AAD: TC41_3262(araA)
LLK: LLKF_1616(araA)
LLX: NCDO2118_1517(araA)
LLJ: LG36_0874(araA)
LPK: LACPI_1209(araA)
LPL: lp_3554(araA)
LPJ: JDM1_2839(araA)
LPS: LPST_C2903(araA)
LSA: LCA_1856(araA)
LBR: LVIS_1740
LRE: Lreu_0482
LRF: LAR_0470
LRU: HMPREF0538_21705(araA)
LRT: LRI_1435
LFE: LAF_1554
LFR: LC40_0980
LFF: LBFF_1712
LBN: LBUCD034_2028(araA-C) LBUCD034_2029(araA-N) LBUCD034_2198(araA)
LHO: LOOC260_120430(araA)
PPE: PEPE_0165
PPEN: T256_00955
EFC: EFAU004_02339(araA2)
EFAU: EFAU085_02403(araA2)
EFU: HMPREF0351_12332(araA)
EFM: M7W_2351
ECAS: ECBG_01552
EMU: EMQU_1838(araA)
MPS: MPTP_0459
MPX: MPD5_1448
THL: TEH_04380(araA)
LME: LEUM_0852
LMM: MI1_03965
LMK: LMES_0778
LCI: LCK_01185(araA)
LKI: LKI_03235
LGS: LEGAS_0270(araA)
WKO: WKK_01700
CRN: CAR_c02880(araA)
CARC: NY10_1502
CAC: CA_C1342(araA) CA_C1346(araA)
CAE: SMB_G1365(araA1) SMB_G1369(araA2)
CAY: CEA_G1356(araA) CEA_G1360
CBE: Cbei_4457
CBZ: Cbs_4457
CBEI: LF65_04992
CLJ: CLJU_c01200(araA)
CSR: Cspa_c46940(araA)
CSB: CLSA_c07560(araA)
CSQ: CSCA_3592
CCE: Ccel_1033
CSS: Cst_c16190(araA)
CSD: Clst_1561(araA)
ESR: ES1_23430
RUM: CK1_21830
BHU: bhn_I1059
RIX: RO1_27560
RIM: ROI_39010
CCT: CC1_27640
RTO: RTO_26010
CPY: Cphy_0584
CSO: CLS_30510
BPRL: CL2_09380
HSD: SD1D_0125(araA)
OVA: OBV_11920(araA)
SAY: TPY_1910(araA)
TIT: Thit_1704
TTM: Tthe_0666
TSH: Tsac_1283
SRI: SELR_27320(araA)
MHG: MHY_04340
PUF: UFO1_3750
PFT: JBW_02637
MSM: MSMEG_1715(araA)
MPHL: MPHLCCUG_01559(araA)
MVQ: MYVA_2122(araA)
ASD: AS9A_2231
RHB: NY08_2092
RFA: A3L23_00426(araA_1)
RHS: A3Q41_02981(araA_2)
SALB: XNR_3312
SGB: WQO_03035
SCT: SCAT_p1155(araA)
SBH: SBI_02270
SVE: SVEN_6729
SRN: A4G23_00451(araA)
SMAL: SMALA_7917
KSK: KSE_74170(araA)
CMI: CMM_0878(araA)
CMS: CMS0133(araA)
CMC: CMN_00838(araA)
MTS: MTES_2217(araA)
ART: Arth_0224
ARR: ARUE_c38440(araA)
ARM: ART_1991
ARX: ARZXY2_46(araA)
AAU: AAur_3711(araA)
ACH: Achl_0381
AAI: AARI_33660(araA)
JDE: Jden_0498
XCE: Xcel_0403
IDO: I598_1470(araA)
CFL: Cfla_2753
CFI: Celf_0872
PFR: PFREUD_06610(araA)
PFRE: RM25_0621
ACIJ: JS278_01368(araA)
MPH: MLP_15140(araA)
NCA: Noca_0382
KFL: Kfla_0263
NDA: Ndas_0751
ACE: Acel_0873
GOB: Gobs_0244
MMAR: MODMU_0253(araA)
AMD: AMED_4404(araA) AMED_4546(araA)
AMM: AMES_4350(araA) AMES_4491(araA)
AMZ: B737_4350(araA) B737_4491(araA)
AOI: AORI_4313(araA)
AJA: AJAP_17335(araA)
AMQ: AMETH_2140(araA)
PSEA: WY02_00530
PSEH: XF36_13425
AMI: Amir_1863
MIL: ML5_4725
ASE: ACPL_5120(araA) ACPL_5441(araA)
ACTN: L083_4938(araA)
CAI: Caci_4713
BLO: BL0272(araA)
BLJ: BLD_1018(araA)
BLF: BLIF_0336
BLL: BLJ_0403(araA)
BLB: BBMN68_1034(araA)
BLM: BLLJ_0342
BLG: BIL_15280
BAD: BAD_1410(araA)
BADL: BADO_1621
BLA: BLA_0058(araA)
BBB: BIF_01646
BBC: BLC1_0061
BLV: BalV_0066
BLW: W7Y_0066
BLS: W91_0065
BANI: Bl12_0058
BANL: BLAC_00285
BANM: EN10_00345
BDE: BDP_1922(araA)
BDN: BBDE_1813
BAST: BAST_0366
BCOR: BCOR_0305
BKS: BBKW_1657
BPSP: AH67_00315
BANG: BBAG_0211
BCAT: BBCT_1454
BPSC: BBPC_1562
BSCA: BBSC_2140
GVG: HMPREF0421_20374(araA)
GVH: HMPREF9231_1181(araA)
PDO: PSDT_0307
CWO: Cwoe_4813
HAU: Haur_3664
ATM: ANT_15070
TRA: Trad_1489
TTR: Tter_2038
OTE: Oter_1382
OBG: Verru16b_01255(araA)
TTF: THTE_1665
PBU: L21SP3_00781(araA)
PBP: STSP1_00182(araA)
GBA: J421_5117
BTHO: Btheta7330_03027(araA)
BVU: BVU_2320
BXY: BXY_15600
BOA: Bovatus_00208(araA)
PSAC: PSM36_0899(araA)
PRU: PRU_0420(araA)
DORI: FH5T_00645
CPI: Cpin_3996
FLN: FLA_1868
SMIZ: 4412673_03644(araA)
LBY: Lbys_2508
FAE: FAES_2721
HSW: Hsw_2681
GFO: GFO_0699(araA)
GFL: GRFL_2267
FJO: Fjoh_1121
FJG: BB050_01482(araA)
ZPR: ZPR_2148
ZGA: ZOBELLIA_2001(araA)
CHZ: CHSO_3292(araA)
AALG: AREALGSMS7_03047(araA)
SPON: HME9304_00690(araA)
MARF: CJ739_925
MRO: MROS_0028
TMA: TM0276
TMW: THMA_0283
TMQ: THMB_0283
TMX: THMC_0283
TPT: Tpet_0648
TRQ: TRQ2_0672
TNA: CTN_0409
TNP: Tnap_0906
DTN: DTL3_0424(araA)
MINF: MESINF_1469(araA)
HUT: Huta_1154
 » show all
Taxonomy
Reference
1  [PMID:13539034]
  Authors
HEATH EC, HORECKER BL, SMYRNIOTIS PZ, TAKAGI Y.
  Title
Pentose fermentation by Lactobacillus plantarum. II. L-arabinose isomerase.
  Journal
J Biol Chem 231:1031-7 (1958)
Reference
2  [PMID:4878429]
  Authors
Patrick JW, Lee N
  Title
Purification and properties of an L-arabinose isomerase from Escherichia coli.
  Journal
J Biol Chem 243:4312-8 (1968)
Reference
3  [PMID:5773448]
  Authors
Nakamatu T, Yamanaka K.
  Title
Crystallization and properties of L-arabinose isomerase from Lactobacillus gayonii.
  Journal
Biochim Biophys Acta 178:156-65 (1969)
DOI:10.1016/0005-2744(69)90142-9
Reference
4
  Authors
Cheetham, P.S.J. and Wootton, A.N.
  Title
Bioconversion of D-galactose into D-tagatose.
  Journal
Enzyme and Microbial Technology 15:105-108 (1993)
Reference
5  [PMID:8869631]
  Authors
Banerjee S, Anderson F, Farber GK
  Title
The evolution of sugar isomerases.
  Journal
Protein Eng 8:1189-95 (1995)
DOI:10.1093/protein/8.12.1189
Reference
6  [PMID:16756997]
  Authors
Manjasetty BA, Chance MR
  Title
Crystal structure of Escherichia coli L-arabinose isomerase (ECAI), the putative  target of biological tagatose production.
  Journal
J Mol Biol 360:297-309 (2006)
DOI:10.1016/j.jmb.2006.04.040
  Sequence
[eco:b0062]
Other DBs
ExplorEnz - The Enzyme Database: 5.3.1.4
IUBMB Enzyme Nomenclature: 5.3.1.4
ExPASy - ENZYME nomenclature database: 5.3.1.4
BRENDA, the Enzyme Database: 5.3.1.4
CAS: 9023-80-7

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