KEGG   ENZYME: 5.3.3.13Help
Entry
EC 5.3.3.13                 Enzyme                                 

Name
polyenoic fatty acid isomerase;
PFI;
eicosapentaenoate cis-Delta5,8,11,14,17-eicosapentaenoate cis-Delta5-trans-Delta7,9-cis-Delta14,17 isomerase;
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate Delta8,11-Delta7,8-isomerase (incorrect);
(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate Delta8,11-Delta7,9-isomerase (trans-double-bond-forming)
Class
Isomerases;
Intramolecular oxidoreductases;
Transposing C=C bonds
BRITE hierarchy
Sysname
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate Delta8,11-Delta7,9-isomerase (trans-double-bond-forming)
Reaction(IUBMB)
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate = (5Z,7E,9E,14Z,17Z)-icosapentaenoate [RN:R06502]
Reaction(KEGG)
Substrate
(5Z,8Z,11Z,14Z,17Z)-icosapentaenoate [CPD:C06428]
Product
(5Z,7E,9E,14Z,17Z)-icosapentaenoate [CPD:C12083]
Comment
The enzyme from the red alga Ptilota filicina catalyses the isomerization of skip dienes (methylene-interrupted double bonds) in a broad range of fatty acids and fatty-acid analogues, such as arachidonate and gamma-linolenate, to yield a conjugated triene.
History
EC 5.3.3.13 created 2004
Orthology
K20706  polyenoic fatty acid isomerase
Reference
1  [PMID:7803384]
  Authors
Wise ML, Hamberg M, Gerwick WH.
  Title
Biosynthesis of conjugated triene-containing fatty acids by a novel isomerase from the red marine alga Ptilota filicina.
  Journal
Biochemistry 33:15223-32 (1994)
  Sequence
Reference
2  [PMID:8575565]
  Authors
Wise ML, Soderstrom K, Murray TF, Gerwick WH.
  Title
Synthesis and cannabinoid receptor binding activity of conjugated triene anandamide, a novel eicosanoid.
  Journal
Experientia 52:88-92 (1996)
Reference
3  [PMID:9062129]
  Authors
Wise ML, Rossi J, Gerwick WH.
  Title
Characterization of the substrate binding site of polyenoic fatty acid isomerase, a novel enzyme from the marine alga Ptilota filicina.
  Journal
Biochemistry 36:2985-92 (1997)
DOI:10.1021/bi962158v
  Sequence
Reference
4  [PMID:12054482]
  Authors
Zheng W, Wise ML, Wyrick A, Metz JG, Yuan L, Gerwick WH.
  Title
Polyenoic fatty acid isomerase from the marine alga Ptilota filicina: protein characterization and functional expression of the cloned cDNA.
  Journal
Arch Biochem Biophys 401:11-20 (2002)
DOI:10.1016/S0003-9861(02)00002-4
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.3.3.13
IUBMB Enzyme Nomenclature: 5.3.3.13
ExPASy - ENZYME nomenclature database: 5.3.3.13
BRENDA, the Enzyme Database: 5.3.3.13
CAS: 159002-84-3

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