KEGG   ENZYME: 5.4.99.39Help
Entry
EC 5.4.99.39                Enzyme                                 

Name
beta-amyrin synthase;
2,3-oxidosqualene beta-amyrin cyclase;
AsbAS1;
BPY;
EtAS;
GgbAS1;
LjAMY1;
MtAMY1;
PNY;
BgbAS
Class
Isomerases;
Intramolecular transferases;
Transferring other groups
BRITE hierarchy
Sysname
(3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, beta-amyrin-forming)
Reaction(IUBMB)
(3S)-2,3-epoxy-2,3-dihydrosqualene = beta-amyrin [RN:R06469]
Reaction(KEGG)
Substrate
(3S)-2,3-epoxy-2,3-dihydrosqualene [CPD:C01054]
Product
beta-amyrin [CPD:C08616]
Comment
Some organism possess a monofunctional beta-amyrin synthase [3,4,6-11], other have a multifunctional enzyme that also catalyses the synthesis of alpha-amyrin (EC 5.4.99.40) [5] or lupeol (EC 5.4.99.41) [6].
History
EC 5.4.99.39 created 2011
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K15813  beta-amyrin synthase
K15815  lupeol synthase 2
K20658  alpha/beta-amyrin synthase
K21928  delta/alpha/beta-amyrin synthase
Genes
ATH: AT1G78950(BAS) AT1G78960(LUP2)
ALY: ARALYDRAFT_894488 ARALYDRAFT_895800
CRB: 17894179 17894180
CSAT: 104714177
EUS: EUTSA_v10018166mg EUTSA_v10022563mg
BRP: 103830506 103832374
BNA: 106354658
DZI: 111280462
GMX: 100778398 100781065 100809670 100810544 547702(AMS1)
VRA: 106779110
MDM: 103443364(OSC4)
RCU: 8259940
POP: 7465577
SLY: 100529100(TTS2) 100529102(lTTS1) 101262591
 » show all
Taxonomy
Reference
1
  Authors
Abe, I, Ebizuka, Y., Seo, S. and Sankawa, U.
  Title
Purification of squalene-2,3-epoxide cyclases from cell suspension cultures of Rabdosia japonica Hara.
  Journal
FEBS Lett 249:100-104 (1989)
Reference
2
  Authors
Abe, I., Sankawa, U. and Ebizuka, Y.
  Title
Purification of 2,3-oxdosqualene:beta-amyrin cyclase from pea seedlings.
  Journal
Chem Pharm Bull 37:536 (1989)
Reference
3  [PMID:9746369]
  Authors
Kushiro T, Shibuya M, Ebizuka Y
  Title
Beta-amyrin synthase--cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants.
  Journal
Eur J Biochem 256:238-44 (1998)
DOI:10.1046/j.1432-1327.1998.2560238.x
  Sequence
Reference
4  [PMID:11510484]
  Authors
Hayashi H, Huang P, Kirakosyan A, Inoue K, Hiraoka N, Ikeshiro Y, Kushiro T, Shibuya M, Ebizuka Y
  Title
Cloning and characterization of a cDNA encoding beta-amyrin synthase involved in  glycyrrhizin and soyasaponin biosyntheses in licorice.
  Journal
Biol Pharm Bull 24:912-6 (2001)
  Sequence
Reference
5  [PMID:11247608]
  Authors
Husselstein-Muller T, Schaller H, Benveniste P
  Title
Molecular cloning and expression in yeast of 2,3-oxidosqualene-triterpenoid cyclases from Arabidopsis thaliana.
  Journal
Plant Mol Biol 45:75-92 (2001)
  Sequence
Reference
6  [PMID:12683345]
  Authors
Iturbe-Ormaetxe I, Haralampidis K, Papadopoulou K, Osbourn AE
  Title
Molecular cloning and characterization of triterpene synthases from Medicago truncatula and Lotus japonicus.
  Journal
Plant Mol Biol 51:731-43 (2003)
Reference
7  [PMID:12736505]
  Authors
Zhang H, Shibuya M, Yokota S, Ebizuka Y
  Title
Oxidosqualene cyclases from cell suspension cultures of Betula platyphylla var. japonica: molecular evolution of oxidosqualene cyclases in higher plants.
  Journal
Biol Pharm Bull 26:642-50 (2003)
  Sequence
Reference
8  [PMID:15256745]
  Authors
Hayashi H, Huang P, Takada S, Obinata M, Inoue K, Shibuya M, Ebizuka Y
  Title
Differential expression of three oxidosqualene cyclase mRNAs in Glycyrrhiza glabra.
  Journal
Biol Pharm Bull 27:1086-92 (2004)
  Sequence
Reference
9  [PMID:16005035]
  Authors
Kajikawa M, Yamato KT, Fukuzawa H, Sakai Y, Uchida H, Ohyama K
  Title
Cloning and characterization of a cDNA encoding beta-amyrin synthase from petroleum plant Euphorbia tirucalli L.
  Journal
Phytochemistry 66:1759-66 (2005)
DOI:10.1016/j.phytochem.2005.05.021
Reference
10 [PMID:17803686]
  Authors
Basyuni M, Oku H, Tsujimoto E, Kinjo K, Baba S, Takara K
  Title
Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae.
  Journal
FEBS J 274:5028-42 (2007)
DOI:10.1111/j.1742-4658.2007.06025.x
  Sequence
Reference
11 [PMID:19420748]
  Authors
Liu Y, Cai Y, Zhao Z, Wang J, Li J, Xin W, Xia G, Xiang F
  Title
Cloning and Functional Analysis of a beta-amyrin synthase gene associated with oleanolic acid biosynthesis in Gentiana straminea MAXIM.
  Journal
Biol Pharm Bull 32:818-24 (2009)
Other DBs
ExplorEnz - The Enzyme Database: 5.4.99.39
IUBMB Enzyme Nomenclature: 5.4.99.39
ExPASy - ENZYME nomenclature database: 5.4.99.39
BRENDA, the Enzyme Database: 5.4.99.39

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