Entry
Name
halimadienyl-diphosphate synthase;
Rv3377c;
halimadienyl diphosphate synthase;
tuberculosinol diphosphate synthase;
halima-5(6),13-dien-15-yl-diphosphate lyase (cyclizing);
halima-5,13-dien-15-yl-diphosphate lyase (decyclizing)
Class
Isomerases;
Intramolecular lyases;
Intramolecular lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
halima-5,13-dien-15-yl-diphosphate lyase (ring-opening)
Reaction(IUBMB)
geranylgeranyl diphosphate = tuberculosinyl diphosphate [RN:
R09715 ]
Reaction(KEGG)
Substrate
geranylgeranyl diphosphate [CPD:
C00353 ]
Product
tuberculosinyl diphosphate [CPD:
C19836 ]
Comment
Requires Mg2+ for activity. This enzyme is found in pathogenic prokaryotes such as Mycobacterium tuberculosis but not in non-pathogens such as Mycobacterium smegmatis so may play a role in pathogenicity. The product of the reaction is subsequently dephosphorylated yielding tuberculosinol (halima-5,13-dien-15-ol).
History
EC 5.5.1.16 created 2008, modified 2012
Orthology
K17811 halimadienyl-diphosphate synthase
Genes
KUT : JJ691_32450 JJ691_60870
» show all
Taxonomy
Reference
Authors
Nakano C, Okamura T, Sato T, Dairi T, Hoshino T
Title
Mycobacterium tuberculosis H37Rv3377c encodes the diterpene cyclase for producing the halimane skeleton.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.5.1.16
ExPASy - ENZYME nomenclature database: 5.5.1.16