KEGG   ENZYME: 6.1.1.26Help
Entry
EC 6.1.1.26                 Enzyme                                 

Name
pyrrolysine---tRNAPyl ligase;
PylS;
pyrrolysyl-tRNA synthetase
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
BRITE hierarchy
Sysname
L-pyrrolysine:tRNAPyl ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-pyrrolysine + tRNAPyl = AMP + diphosphate + L-pyrrolysyl-tRNAPyl [RN:R08578]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-pyrrolysine [CPD:C16138];
tRNA(Pyl) [CPD:C16139]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-pyrrolysyl-tRNA(Pyl) [CPD:C17027]
Comment
In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNAPyl, with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl---tRNA ligase. The enzyme is specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids [1].
History
EC 6.1.1.26 created 2007
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K11627  pyrrolysyl-tRNA synthetase
Genes
MAC: MA_0155
MBA: Mbar_A0839
MBY: MSBRM_0155
MBW: MSBRW_0162
MBAR: MSBR2_2226
MBAK: MSBR3_2277
MMA: MM_1445
MMJ: MSMAS_3219
MMAC: MSMAC_3241
MVC: MSVAZ_3361
METM: MSMTP_3119
MTHE: MSTHC_1349
MTHR: MSTHT_1934
MHOR: MSHOH_3965
MBU: Mbur_2086
MMET: MCMEM_0285
MMH: Mmah_0283
MHAZ: BHR79_02105(pylS)
MPY: Mpsy_1721(pylS)
 » show all
Taxonomy
Reference
1  [PMID:15329732]
  Authors
Blight SK, Larue RC, Mahapatra A, Longstaff DG, Chang E, Zhao G, Kang PT, Green-Church KB, Chan MK, Krzycki JA.
  Title
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo.
  Journal
Nature 431:333-5 (2004)
DOI:10.1038/nature02895
  Sequence
Reference
2  [PMID:15314242]
  Authors
Polycarpo C, Ambrogelly A, Berube A, Winbush SM, McCloskey JA, Crain PF, Wood JL, Soll D.
  Title
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.
  Journal
Proc Natl Acad Sci U S A 101:12450-4 (2004)
DOI:10.1073/pnas.0405362101
  Sequence
Reference
3  [PMID:15372017]
  Authors
Schimmel P, Beebe K.
  Title
Molecular biology: genetic code seizes pyrrolysine.
  Journal
Nature 431:257-8 (2004)
DOI:10.1038/431257a
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.26
IUBMB Enzyme Nomenclature: 6.1.1.26
ExPASy - ENZYME nomenclature database: 6.1.1.26
BRENDA, the Enzyme Database: 6.1.1.26

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