KEGG   ENZYME: 6.1.1.27Help
Entry
EC 6.1.1.27                 Enzyme                                 

Name
O-phospho-L-serine---tRNA ligase;
O-phosphoseryl-tRNA ligase;
non-canonical O-phosphoseryl-tRNA synthetase;
SepRS
Class
Ligases;
Forming carbon-oxygen bonds;
Ligases forming aminoacyl-tRNA and related compounds
BRITE hierarchy
Sysname
O-phospho-L-serine:tRNACys ligase (AMP-forming)
Reaction(IUBMB)
ATP + O-phospho-L-serine + tRNACys = AMP + diphosphate + O-phospho-L-seryl-tRNACys [RN:R08576]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
O-phospho-L-serine [CPD:C01005];
tRNA(Cys) [CPD:C01639]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
O-phospho-L-seryl-tRNACys
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73 (O-phospho-L-seryl-tRNA: Cys-tRNA synthase) [2].
History
EC 6.1.1.27 created 2009
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K07587  O-phosphoseryl-tRNA synthetase
Genes
MJA: MJ_1660
MFE: Mefer_0735
MVU: Metvu_1155
MFS: MFS40622_0592
MIF: Metin_0235
MJH: JH146_1468
MIG: Metig_1346
MMP: MMP0688
MMQ: MmarC5_0888
MMX: MmarC6_0196
MMD: GYY_02815
MAE: Maeo_1060
MVO: Mvol_0433
MTH: MTH_1501
MMG: MTBMA_c00860(pheS1)
METC: MTCT_1365
MWO: MWSIV6_0042(sepS)
METE: tca_01448(pheS_2)
METH: MBMB1_0036(sepS)
MFC: BRM9_0091(sepS)
MFI: DSM1535_1409(sepS)
MCUB: MCBB_0052(sepS)
MFV: Mfer_0342
MKA: MK0439
AFU: AF_0110
FPL: Ferp_0952
GAC: GACE_0149
GAH: GAH_00998
MAC: MA_0090(pheRS)
MBAR: MSBR2_3396
MBAK: MSBR3_3408
MMA: MM_1383
MMAC: MSMAC_3298
METM: MSMTP_3173
MTHE: MSTHC_1401
MTHR: MSTHT_1883
MHOR: MSHOH_4028
MBU: Mbur_0923
MMET: MCMEM_1431
MMH: Mmah_0685
MPY: Mpsy_1453
MTP: Mthe_0363
MCJ: MCON_3135(sepS)
MHI: Mhar_0928
MHU: Mhun_2839
MLA: Mlab_0537
MEMA: MMAB1_1853(sepS)
MPI: Mpet_0614
MBN: Mboo_0273
MPL: Mpal_0290
MPD: MCP_1457
MEZ: Mtc_1161
RCI: RRC273
BARB: AOA66_0046(sepS)
 » show all
Taxonomy
Reference
1  [PMID:17351629]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
  Journal
Nat Struct Mol Biol 14:272-9 (2007)
DOI:10.1038/nsmb1219
  Sequence
[mja:MJ_1660] [afu:AF_0110]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J Biol Chem 283:22007-17 (2008)
DOI:10.1074/jbc.M801839200
Other DBs
ExplorEnz - The Enzyme Database: 6.1.1.27
IUBMB Enzyme Nomenclature: 6.1.1.27
ExPASy - ENZYME nomenclature database: 6.1.1.27
BRENDA, the Enzyme Database: 6.1.1.27

DBGET integrated database retrieval system