KEGG   ENZYME: 6.2.1.7
Entry
EC 6.2.1.7                  Enzyme                                 
Name
cholate---CoA ligase;
BAL;
bile acid CoA ligase;
bile acid coenzyme A ligase;
choloyl-CoA synthetase;
choloyl coenzyme A synthetase;
cholic thiokinase;
cholate thiokinase;
cholic acid:CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase;
THCA-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate---CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming);
cholyl-CoA synthetase;
trihydroxycoprostanoyl-CoA synthetase
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
cholate:CoA ligase (AMP-forming)
Reaction(IUBMB)
(1) ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA [RN:R02794];
(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA [RN:R04580]
Reaction(KEGG)
R02794 R04580;
(other) R04507 R08733 R08738 R08743
Substrate
ATP [CPD:C00002];
cholate [CPD:C00695];
CoA [CPD:C00010];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
choloyl-CoA [CPD:C01794];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA
Comment
Requires Mg2+ for activity. The mammalian enzyme is membrane-bound and catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [7]. The bacterial enzyme is soluble and participates in an anaerobic bile acid 7 alpha-dehydroxylation pathway [5].
History
EC 6.2.1.7 created 1961 (EC 6.2.1.29 created 1992, incorporated 2005), modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00121  Secondary bile acid biosynthesis
ec01100  Metabolic pathways
Orthology
K08748  solute carrier family 27 (fatty acid transporter), member 5
K15868  bile acid-coenzyme A ligase
Genes
HSA: 10998(SLC27A5)
PTR: 456351(SLC27A5)
PPS: 100973518(SLC27A5)
GGO: 101141402(SLC27A5)
PON: 100450572(SLC27A5)
PPYG: 129020656(SLC27A5)
NLE: 100598523(SLC27A5)
HMH: 116479284(SLC27A5)
SSYN: 129460766(SLC27A5)
MCC: 713713(SLC27A5)
MCF: 102139611(SLC27A5)
MTHB: 126941889
MNI: 105488041(SLC27A5)
CSAB: 103235458(SLC27A5)
CATY: 105593284(SLC27A5)
PANU: 101012361(SLC27A5)
TGE: 112612525(SLC27A5)
MLEU: 105531198(SLC27A5)
RRO: 104680867(SLC27A5)
RBB: 108514368(SLC27A5)
TFN: 117091868(SLC27A5)
PTEH: 111530345(SLC27A5)
CANG: 105524030(SLC27A5)
CJC: 100394591(SLC27A5)
SBQ: 101033269(SLC27A5)
CIMI: 108289969(SLC27A5)
ANAN: 105731437(SLC27A5)
CSYR: 103262310(SLC27A5)
MMUR: 105864502(SLC27A5)
LCAT: 123624765(SLC27A5)
PCOQ: 105819607(SLC27A5)
OGA: 100943998(SLC27A5)
MMU: 26459(Slc27a5)
MCAL: 110297223(Slc27a5)
MPAH: 110336881(Slc27a5)
RNO: 79111(Slc27a5)
MCOC: 116101654(Slc27a5)
ANU: 117700681(Slc27a5)
ASYL: 127675056(Slc27a5)
MUN: 110562939(Slc27a5)
CGE: 100765567(Slc27a5)
MAUA: 101824762(Slc27a5)
PROB: 127238975(Slc27a5)
PLEU: 114703883(Slc27a5)
MORG: 121449385(Slc27a5)
MFOT: 126493814
AAMP: 119820463(Slc27a5)
NGI: 103725476(Slc27a5)
HGL: 101724180(Slc27a5)
CPOC: 100717817(Slc27a5)
CCAN: 109683037(Slc27a5)
DORD: 105994312(Slc27a5)
DSP: 122110673(Slc27a5)
DMER: 138812659(Slc27a5)
PLOP: 125338761(Slc27a5)
NCAR: 124966722
MMMA: 107158986(Slc27a5)
ITI: 101959603(Slc27a5)
OCU: 100340287
OPI: 101527096(SLC27A5)
TUP: 102483776(SLC27A5)
GVR: 103595548(SLC27A5)
CFA: 608675(SLC27A5)
CLUD: 112643327(SLC27A5)
VVP: 112908791(SLC27A5)
VLG: 121484156(SLC27A5)
NPO: 129500979(SLC27A5)
AML: 100478693(SLC27A5)
UMR: 103677216(SLC27A5)
UAH: 113248017(SLC27A5)
UAR: 123776624(SLC27A5)
ELK: 111139861
LLV: 125089011
MPUF: 101687342(SLC27A5)
MNP: 132005114(SLC27A5)
MLK: 131818144(SLC27A5)
NVS: 122912093(SLC27A5)
ORO: 101381954(SLC27A5)
EJU: 114200965(SLC27A5)
ZCA: 113936449(SLC27A5)
MLX: 117997921(SLC27A5)
NSU: 110581926(SLC27A5)
LWW: 102737843(SLC27A5)
FCA: 101086686(SLC27A5)
PYU: 121023395(SLC27A5)
PCOO: 112850703(SLC27A5)
PBG: 122494649(SLC27A5)
PVIV: 125152405(SLC27A5)
LRUF: 124522780
LGF: 123577870(SLC27A5)
AJU: 106987933
PTG: 102948587(SLC27A5)
PPAD: 109260425(SLC27A5)
PUC: 125915971
PLEZ: 122207842(SLC27A5)
HHV: 120227093(SLC27A5)
BTA: 533016(SLC27A5)
BOM: 102285844(SLC27A5)
BIU: 109571897(SLC27A5)
BBUB: 102396712(SLC27A5)
BBIS: 105001638(SLC27A5)
CHX: 102172839(SLC27A5)
OAS: 101120689(SLC27A5)
BTAX: 128063061(SLC27A5)
CSUM: 138096613(SLC27A5)
ODA: 120871847(SLC27A5)
CCAD: 122420603(SLC27A5)
MREE: 136161857(SLC27A5)
OVR: 110140997(SLC27A5)
MBEZ: 129546342(SLC27A5)
SSC: 102158419(SLC27A5)
CFR: 102519177(SLC27A5)
CBAI: 105074455(SLC27A5)
CDK: 105096797(SLC27A5)
VPC: 102538658(SLC27A5)
BACU: 103020672(SLC27A5)
BMUS: 118884939(SLC27A5)
LVE: 103086085(SLC27A5)
OOR: 101280268(SLC27A5)
LALB: 132509658(SLC27A5)
GMF: 115848061(SLC27A5)
DLE: 111170038(SLC27A5)
PCAD: 102979842(SLC27A5)
PSIU: 116744807(SLC27A5)
PPHC: 136140717(SLC27A5)
NASI: 112396914(SLC27A5)
ECB: 100051678(SLC27A5)
EPZ: 103567891(SLC27A5)
EAI: 106846663(SLC27A5)
MYB: 102261821(SLC27A5)
MYD: 102751593(SLC27A5)
MMYO: 118657417(SLC27A5)
MLF: 102425294(SLC27A5)
MDT: 132216415(SLC27A5)
MYUM: 139000186(SLC27A5)
PKL: 118712387(SLC27A5)
EFUS: 103304167(SLC27A5)
MNA: 107539367(SLC27A5)
DRO: 112313581(SLC27A5)
SHON: 118988175(SLC27A5)
AJM: 119051596(SLC27A5)
PDIC: 114511678(SLC27A5)
PHAS: 123825485(SLC27A5)
MMF: 118638221(SLC27A5)
PPAM: 129066277(SLC27A5)
HAI: 109377097(SLC27A5)
RFQ: 117034578(SLC27A5)
PALE: 102890200(SLC27A5)
PGIG: 120607133(SLC27A5)
PVP: 105308648(SLC27A5)
RAY: 107500402(SLC27A5)
MJV: 108408237(SLC27A5)
TOD: 119249800(SLC27A5)
SARA: 101552573(SLC27A5)
SFUM: 130043874(SLC27A5)
SETR: 126027979(SLC27A5)
LAV: 100676206(SLC27A5)
TMU: 101361902
ETF: 101643777(SLC27A5)
DNM: 101438816(SLC27A5)
MDO: 100029941(SLC27A5)
GAS: 123242899(SLC27A5)
SHR: 100916639(SLC27A5)
AFZ: 127556609
PCW: 110220184(SLC27A5)
TVP: 118838129(SLC27A5)
PBRV: 138173395(SLC27A5)
OAA: 100090562(SLC27A5)
LPIC: 129261741
RPHI: 132721510
KIM: G3T16_11475 G3T16_16845
SDF: ACG33_01260
DOE: DENOEST_3894
DENI: B9N43_12455
PLA: Plav_1025 Plav_1029 Plav_1034
RBS: RHODOSMS8_00086(baiB)
BJA: bll0131(bll0131)
BJU: BJ6T_01450
BJP: RN69_00685
BRA: BRADO6529
BBT: BBta_1112
BRS: S23_06710
AOL: S58_10890
BRC: BCCGELA001_03190
BIC: LMTR13_34780
BRO: BRAD285_6438
BRK: CWS35_11230
BOT: CIT37_14150
BRQ: CIT40_01725
BGQ: X265_03260
BGZ: XH91_04175
BSYM: CIT39_00180
BBET: F8237_17610
BARH: WN72_46090
BVZ: BRAD3257_0642
BEL: BE61_86530
BDG: LPJ38_06565
BSEP: HAP48_0020605
BQB: J4P68_0013450
BSEI: KMZ68_04495
BBAN: J4G43_050000
BCAN: BcanWSM471_03240 BcanWSM471_08330
BAUT: QA635_37450
BBRA: QA636_38525
BGK: IC762_05100
BCOU: IC761_03335
BPAH: QA639_36935
BXN: I3J27_03165 I3J27_33625 I3J27_33655
BROS: QUH67_31575
BRAZ: LRP30_42785
BRAY: ML401_02375
BRAI: FJV43_20255
BRAA: NL528_06880
BLUP: AB3L03_12040 AB3L03_19700
RHOL: RBJ75_26510
VGO: GJW-30_1_02490(baiB_2)
TALZ: RPMA_07725
XDI: EZH22_21750
RBM: TEF_16880
CAK: Caul_3247 Caul_4604
CAUB: AMEJIAPC_03607(baiB)
PZU: PHZ_c2517
CAUL: KCG34_17910
PHEY: O4N75_20590
BNE: DA69_06025
BREA: HZ989_08370
BREU: FKQ52_02150
BSTA: ACETK8_05690 ACETK8_07985
BREN: KKHFBJBL_00373(baiB)
TSV: DSM104635_02995 DSM104635_03075
YAN: AYJ57_19780
TMD: KUV46_00545
RSU: NHU_03725
RHM: B5V46_17095
HNE: HNE_0099(baiB)
NGF: FRF71_13160
SMAZ: LH19_12220 LH19_27280
SPHO: C3E99_13085
SPJJ: V6U71_07365 V6U71_13560
SWI: Swit_0351 Swit_1599 Swit_1600
SPHD: HY78_17585 HY78_17590 HY78_17610 HY78_26355
RDI: CMV14_03665
SJP: SJA_C2-03890 SJA_C2-04050
SCH: Sphch_3661
SPMI: K663_20463
SPHT: K426_28370
SPJS: NUH86_21915
CIJ: WG74_11935
NAO: Y958_27085
CSCI: HDCHBGLK_01430(baiB)
CHYL: CE91St63_15040
CBIL: EUBC25_24900
PHX: KGNDJEFE_00401(baiB)
ELE: Elen_3099
MPA: MAP_2354
MAV: MAV_1627
MAVM: MAA44156_02676
MIT: OCO_16930 OCO_48560
MIA: OCU_17130 OCU_48510
MID: MIP_02338 MIP_07342
MYO: OEM_14930 OEM_48710
MCHI: AN480_08330 AN480_23315 AN480_25245
MIR: OCQ_14600 OCQ_49570
MMAL: CKJ54_07175 CKJ54_23135
MMAN: MMAN_24730 MMAN_36630
MSER: MTY59_11840 MTY59_33860
MSA: Mycsm_00180
MUL: MUL_3844
MMI: MMAR_3983
MMAE: MMARE11_37900
MLI: MULP_04163
MMC: Mmcs_0223
MKM: Mkms_0233
MJL: Mjls_0213
MMM: W7S_07140 W7S_24315
MKN: MKAN_05880
MYE: AB431_01945
MSHG: MSG_00422 MSG_03568
MFJ: MFLOJ_30860 MFLOJ_51280
MSTO: MSTO_34790 MSTO_55390
MSIM: MSIM_20450 MSIM_40770
MSAK: MSAS_18290
MKU: I2456_02760 I2456_18740
MLW: MJO58_02270 MJO58_19560
MXE: MYXE_17320 MYXE_46350
MNV: MNVI_02600 MNVI_19750
MPAG: C0J29_02725 C0J29_21705
MGOR: H0P51_02740 H0P51_19675
MCOO: MCOO_16060 MCOO_35900
MBAI: MB901379_03460(lcfB_9)
MSEO: MSEO_23560 MSEO_43170
MHEK: JMUB5695_01801 JMUB5695_04479
MGAU: MGALJ_47310
MLJ: MLAC_10490
MBRD: MBRA_12830 MBRA_32660
MMAM: K3U93_16925
MSPG: F6B93_16025
MOT: LTS72_05340
MPAA: MKK62_07880 MKK62_23045
MKY: IWGMT90018_04990 IWGMT90018_41340
MWU: PT015_09070 PT015_19535
MYW: BVC93_07035 BVC93_15435
MYJ: BCA37_27350
MSM: MSMEG_0318
MSG: MSMEI_0312
MSB: LJ00_01600
MSN: LI99_01600
MSH: LI98_01600
MVA: Mvan_0252
MGI: Mflv_0419
MCB: Mycch_0222
MFT: XA26_02940
MPHL: MPHLCCUG_04868(baiB_2)
MVQ: MYVA_0257
MLL: B1R94_01565
MRH: MycrhN_1496
MTHN: 4412656_00248(fadD_2)
MDU: MDUV_11370
MCHT: MCHIJ_07000
MAUU: NCTC10437_00256
MMOR: MMOR_01890 MMOR_58370
MAIC: MAIC_53080
MIJ: MINS_27090
MTY: MTOK_10360
MPSC: MPSYJ_06360
MGAD: MGAD_45630
MHEV: MHEL_18710
MSAR: MSAR_18910
MANY: MANY_43890
MPOF: MPOR_07960
MBOK: MBOE_15230
MFG: K6L26_30000
MFLV: NCTC10271_04880
MCEE: MCEL_29920
MMON: EWR22_01215
MGO: AFA91_08425
MHOL: K3U96_25350
MSEN: K3U95_01455
MPAE: K0O64_01570
MRF: MJO55_01200
MCRO: MI149_02120
MAUS: JN090_18680
MYQ: TUM20985_33560
MAB: MAB_2316
MMV: MYCMA_1247
MABB: MASS_2242
MABL: MMASJCM_2266
MCHE: BB28_11610
MIZ: BAB75_12360
MSTE: MSTE_02250
MSAO: MYCSP_11375
MSAL: DSM43276_02035(baiB)
CGY: CGLY_15810
NBR: O3I_021420
NNO: NONO_c50630
NOZ: DMB37_08020
NYA: LTV02_22920
NVU: V7968_29255
RTM: G4H71_11130
RKO: JWS14_47605
RHOP: D8W71_02125
WHR: OG579_02230
GOM: D7316_03708(baiB_2)
SBH: SBI_00137
STRC: AA958_12300
SGRS: V9K83_30435
SNZ: DC008_01285
SSEO: D0Z67_25560
SBAT: G4Z16_17215
SFIY: F0344_07365
STRV: EZV63_00860
YIA: LO772_03550 LO772_29980 LO772_35175
CPHA: FGI33_11885
CCAF: FGD68_03385
MLIY: RYJ27_03370
MBET: N8K70_04700
MICM: CHE218_18800
MICI: AB2L57_16015
RFS: C1I64_10215
RATH: GSU68_00280
CART: PA27867_2220
BRR: C1N80_03880
PROU: AB1046_07585
CDON: KKR89_09550
NOCQ: QI633_17985
AER: AERYTH_14445
ACTW: F7P10_09660 F7P10_11280
AMAZ: LUW76_14885 LUW76_14955 LUW76_22035 LUW76_25845
SPIQ: OHA34_14875
NOW: GBF35_30420
FRE: Franean1_0614 Franean1_4026
FRI: FraEuI1c_2666 FraEuI1c_3335 FraEuI1c_4447 FraEuI1c_5318 FraEuI1c_5444
SEN: SACE_3387(baiB)
AMYY: YIM_33605(baiB2)
PDX: Psed_0542
PSEA: WY02_09705
PSEE: FRP1_04515
PSEH: XF36_02965
PSEQ: AD006_12175
PECQ: AD017_20000
PAUT: Pdca_52450
PBRO: HOP40_02455
PPEL: H6H00_02995
SSYI: EKG83_31105
KAL: KALB_5626
KPHY: AOZ06_30715
ACTY: OG774_25295
UME: RM788_00940 RM788_48095
MICH: FJK98_11545
VER: HUT12_13430
PLK: CIK06_12190
DFU: Dfulv_32595
DACT: KZZ52_25735 KZZ52_26285
EKE: EK0264_04430
AYM: YM304_25790
ATQ: GH723_15305
AQZ: KSP35_22785
 » show all
Reference
1  [PMID:13303991]
  Authors
ELLIOTT WH.
  Title
The enzymic activation of cholic acid by guinea-pig-liver microsomes.
  Journal
Biochem J 62:427-33 (1956)
DOI:10.1042/bj0620427
Reference
2  [PMID:13403911]
  Authors
ELLIOTT WH.
  Title
The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes.
  Journal
Biochem J 65:315-21 (1957)
DOI:10.1042/bj0650315
Reference
3  [PMID:3183523]
  Authors
Prydz K, Kase BF, Bjorkhem I, Pedersen JI.
  Title
Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver.
  Journal
J Lipid Res 29:997-1004 (1988)
Reference
4  [PMID:2521999]
  Authors
Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP.
  Title
Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver.
  Journal
Biochem J 257:221-9 (1989)
DOI:10.1042/bj2570221
Reference
5  [PMID:1551828]
  Authors
Mallonee DH, Adams JL, Hylemon PB
  Title
The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase.
  Journal
J Bacteriol 174:2065-71 (1992)
DOI:10.1128/JB.174.7.2065-2071.1992
  Sequence
[ag:AAC45410]
Reference
6  [PMID:9390170]
  Authors
Wheeler JB, Shaw DR, Barnes S.
  Title
Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes.
  Journal
Arch Biochem Biophys 348:15-24 (1997)
DOI:10.1006/abbi.1997.0391
  Sequence
[rno:79111]
Reference
7  [PMID:12454267]
  Authors
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.
  Title
Molecular cloning and expression of rat liver bile acid CoA ligase.
  Journal
J Lipid Res 43:2062-71 (2002)
DOI:10.1194/jlr.m200260-jlr200
  Sequence
[rno:79111]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.7
IUBMB Enzyme Nomenclature: 6.2.1.7
ExPASy - ENZYME nomenclature database: 6.2.1.7
BRENDA, the Enzyme Database: 6.2.1.7
CAS: 9027-90-1

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Gene (757)   
   MGENES (481)   
   RefGene (276)   
Protein sequence (72)   
   UniProt (15)   
   SWISS-PROT (7)   
   RefSeq(pep) (50)   
DNA sequence (138)   
   RefSeq(nuc) (125)   
   GenBank (10)   
   EMBL (3)   
Protein domain (7)   
   InterPro (7)   
All databases (974)   

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