| Entry |
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| Name |
cholate---CoA ligase;
BAL;
bile acid CoA ligase;
bile acid coenzyme A ligase;
choloyl-CoA synthetase;
choloyl coenzyme A synthetase;
cholic thiokinase;
cholate thiokinase;
cholic acid:CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase;
THCA-CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate---CoA ligase;
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming);
cholyl-CoA synthetase;
trihydroxycoprostanoyl-CoA synthetase
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| Class |
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
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| Sysname |
cholate:CoA ligase (AMP-forming)
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| Reaction(IUBMB) |
(1) ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA [RN: R02794];
(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA [RN: R04580]
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| Reaction(KEGG) |
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| Substrate |
ATP [CPD: C00002];
cholate [CPD: C00695];
CoA [CPD: C00010];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate
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| Product |
AMP [CPD: C00020];
diphosphate [CPD: C00013];
choloyl-CoA [CPD: C01794];
(25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA
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| Comment |
Requires Mg2+ for activity. The mammalian enzyme is membrane-bound and catalyses the first step in the conjugation of bile acids with amino acids, converting bile acids into their acyl-CoA thioesters. Chenodeoxycholate, deoxycholate, lithocholate and trihydroxycoprostanoate can also act as substrates [7]. The bacterial enzyme is soluble and participates in an anaerobic bile acid 7 alpha-dehydroxylation pathway [5].
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| History |
EC 6.2.1.7 created 1961 (EC 6.2.1.29 created 1992, incorporated 2005), modified 2005
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| Pathway |
| ec00120 | Primary bile acid biosynthesis |
| ec00121 | Secondary bile acid biosynthesis |
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| Orthology |
| K08748 | solute carrier family 27 (fatty acid transporter), member 5 |
| K15868 | bile acid-coenzyme A ligase |
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| Genes |
» show all
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| Reference |
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| Authors |
ELLIOTT WH. |
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| Title |
The enzymic activation of cholic acid by guinea-pig-liver microsomes. |
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| Journal |
Biochem J 62:427-33 (1956) |
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| Reference |
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| Authors |
ELLIOTT WH. |
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| Title |
The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes. |
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| Journal |
Biochem J 65:315-21 (1957) |
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| Reference |
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| Authors |
Prydz K, Kase BF, Bjorkhem I, Pedersen JI. |
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| Title |
Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver. |
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| Journal |
J Lipid Res 29:997-1004 (1988) |
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| Reference |
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| Authors |
Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP. |
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| Title |
Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver. |
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| Journal |
Biochem J 257:221-9 (1989) |
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| Reference |
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| Authors |
Mallonee DH, Adams JL, Hylemon PB |
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| Title |
The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Wheeler JB, Shaw DR, Barnes S. |
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| Title |
Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S. |
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| Title |
Molecular cloning and expression of rat liver bile acid CoA ligase. |
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| Journal |
J Lipid Res 43:2062-71 (2002) |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 6.2.1.7 |
| ExPASy - ENZYME nomenclature database: | 6.2.1.7 |
| BRENDA, the Enzyme Database: | 6.2.1.7 |
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