KEGG   ENZYME: 6.3.1.13Help
Entry
EC 6.3.1.13                 Enzyme                                 

Name
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase;
MshC;
MshC ligase;
Cys:GlcN-Ins ligase;
mycothiol ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy
Sysname
L-cysteine:1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol ligase (AMP-forming)
Reaction(IUBMB)
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol + AMP + diphosphate [RN:R09591]
Reaction(KEGG)
Substrate
1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol [CPD:C19702];
L-cysteine [CPD:C00097];
ATP [CPD:C00002]
Product
1-O-[2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl]-1D-myo-inositol [CPD:C19703];
AMP [CPD:C00020];
diphosphate [CPD:C00013]
Comment
This enzyme is a key enzyme in the biosynthesis of mycothiol, a small molecular weight thiol found in Mycobacteria spp. and other actinomycetes. Mycothiol plays a fundamental role in these organisms by helping to provide protection from the effects of reactive oxygen species and electrophiles, including many antibiotics. The enzyme may represent a novel target for new classes of antituberculars [2].
History
EC 6.3.1.13 created 2009
Orthology
K15526  L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Genes
MTU: Rv2130c(mshC)
MTV: RVBD_2130c
MTC: MT2188(cysS-1)
MRA: MRA_2144(cysS1)
MTF: TBFG_12161
MTB: TBMG_01851
MTK: TBSG_01863
MTZ: TBXG_001834
MTG: MRGA327_13125
MTI: MRGA423_13240
MTUR: CFBS_2257(cysS1)
MTO: MTCTRI2_2165(cysS)
MTD: UDA_2130c(cysS2)
MTN: ERDMAN_2347(cysS)
MTUB: MT7199_2160(mshC)
MTUC: J113_14765
MTUE: J114_11415
MTUL: TBHG_02085
MTUT: HKBT1_2248(cysS1)
MTUU: HKBT2_2250(cysS1)
MTQ: HKBS1_2254(cysS1)
MBO: BQ2027_MB2154C(mshc)
MBB: BCG_2147c(cysS2)
MBT: JTY_2141(cysS2)
MBM: BCGMEX_2133c(cysS2)
MBX: BCGT_1951
MAF: MAF_21420(cysS2)
MMIC: RN08_2361
MCE: MCAN_21541(cysS2)
MCQ: BN44_50059(cysS)
MCV: BN43_31347(cysS)
MCX: BN42_40042(cysS)
MCZ: BN45_50451(cysS)
MLE: ML1302(cysS)
MLB: MLBr01302(cysS2)
MPA: MAP_1876c(cysS2)
MAO: MAP4_1953
MAVI: RC58_09755
MAVU: RE97_09760
MAV: MAV_2361(cysS)
MAVD: NF84_10630
MAVR: LA63_10850
MAVA: LA64_10885
MIT: OCO_23640
MIA: OCU_23520
MID: MIP_03257
MYO: OEM_22060
MIR: OCQ_22160
MLP: MLM_2236
MUL: MUL_2360(mshC)
MMC: Mmcs_3153
MKM: Mkms_3215
MJL: Mjls_3165
MMI: MMAR_3110(mshC)
MMAE: MMARE11_30320(mshC)
MMM: W7S_11410
MLI: MULP_03371(mshC)
MHAD: B586_10540
MSM: MSMEG_4189(cysS)
MSG: MSMEI_4091(cysS2)
MVA: Mvan_3478
MGI: Mflv_3049
MFT: XA26_36370(mshC)
MPHL: MPHLCCUG_03253(mshC)
MVQ: MYVA_3402(cysS1)
MTHN: 4412656_01910(mshC)
MAB: MAB_2116
MABB: MASS_2039
MABO: NF82_10570
MCHE: BB28_10590
MSTE: MSTE_02046(mshC)
MJD: JDM601_1772(mshC)
MTER: 4434518_01718(mshC)
ASD: AS9A_1960(mshC)
CGL: NCgl1457(cysS)
CGB: cg1709(cysS2)
CGU: WA5_1457(CysS)
CGT: cgR_1574
CGM: cgp_1709(mshC)
CGJ: AR0_08110
CEF: CE1639(cysS)
CDI: DIP1261(cysS2)
CDP: CD241_1191(cysS2)
CDH: CDB402_1170(cysS2)
CDT: CDHC01_1190(cysS2)
CDE: CDHC02_1169(cysS2)
CDR: CDHC03_1164(cysS2)
CDA: CDHC04_1174(cysS2)
CDZ: CD31A_1272(cysS2)
CDB: CDBH8_1239(cysS2)
CDS: CDC7B_1255(cysS2)
CDD: CDCE8392_1162(cysS2)
CDW: CDPW8_1240(cysS2)
CDV: CDVA01_1131(cysS2)
CDIP: ERS451417_01261(cysS2)
CJK: jk0368(cysS2) jk0956(cysS1)
CUA: CU7111_1014(mshC)
CAR: cauri_1250(mshC)
CKP: ckrop_0920(mshC)
CPU: cpfrc_01054(mshC)
CPL: Cp3995_1074(mshC)
CPG: Cp316_1095(mshC)
CPP: CpP54B96_1068(mshC)
CPK: Cp1002_1049(mshC)
CPQ: CpC231_1047(mshC)
CPX: CpI19_1054(mshC)
CPZ: CpPAT10_1048(mshC)
COR: Cp267_1100(mshC)
COP: Cp31_1058(mshC)
COD: Cp106_1031(mshC)
COS: Cp4202_1041(mshC)
COI: CpCIP5297_1068(mshC)
COE: Cp258_1066(mshC)
COU: Cp162_1048(mshC)
CPSE: CPTA_01622
CPSU: CPTB_02141
CPSF: CPTC_01613
CRD: CRES_1038(mshC)
CUL: CULC22_01124(mshC)
CUC: CULC809_01109(mshC)
CUE: CULC0102_1233(cysS)
CUN: Cul210932_1165(mshC)
CUS: CulFRC11_1133(mshC)
CUQ: Cul210931_1115(mshC)
CUZ: Cul05146_1189(mshC)
CUJ: CUL131002_1147c(mshC)
CVA: CVAR_1445(mshC)
CTER: A606_05610
CGY: CGLY_08655(mshC2)
COA: DR71_50(mshC)
CMQ: B840_06340(mshC)
CKU: UL82_05260(mshC)
CCJ: UL81_06070(mshC)
CMV: CMUST_08100(mshC)
CEI: CEPID_06495(mshC)
CTED: CTEST_06690(mshC)
CUT: CUTER_05510(mshC)
CDX: CDES_07295(mshC)
CSP: WM42_0105(mshC)
CPHO: CPHO_06025
CGV: CGLAU_06465(mshC)
CAQU: CAQU_06410
CAMG: CAMM_06785
CMIN: NCTC10288_01274(mshC)
NFA: NFA_29340(mshC)
NFR: ERS450000_01160(mshC)
NCY: NOCYR_2775(cysS)
NNO: NONO_c37250(mshC)
NSR: NS506_04748(mshC)
NTP: CRH09_19110(mshC)
RER: RER_31960(mshC)
REY: O5Y_14690
ROP: ROP_06130(mshC)
REQ: REQ_22990(mshC)
RHB: NY08_1030
RFA: A3L23_04280(mshC)
RHS: A3Q41_03986(mshC)
RRZ: CS378_03350(mshC)
RHU: A3Q40_01708(mshC)
RQI: C1M55_15935(mshC)
RRT: 4535765_02337(mshC)
GBR: Gbro_2442
GPO: GPOL_c23790(mshC)
GOR: KTR9_2351
GOC: CXX93_12580(mshC)
GIT: C6V83_11530(mshC)
TPR: Tpau_2140
SRT: Srot_1222
DIT: C3V38_13760(mshC)
DIZ: CT688_07945(mshC)
SCO: SCO1663(cysS)
SALB: XNR_5164
SMA: SAVERM_6647(mshC)
SGR: SGR_5840(cysS2)
SGB: WQO_06085
SCB: SCAB_73151(cysS2)
SCT: SCAT_5134(cysS)
SFA: Sfla_5170
SBH: SBI_08453(cysS2)
SHY: SHJG_3103
SVE: SVEN_1258
SDV: BN159_6915(mshC)
SALS: SLNWT_6236
STRP: F750_1464(mshC)
SFI: SFUL_1182
SALU: DC74_2143
SALL: SAZ_11290
SLV: SLIV_29430(mshC)
SGU: SGLAU_06905(mshC)
STRE: GZL_06994
SLD: T261_6564
STRM: M444_08655
SAMB: SAM23877_1720(mshC)
SPRI: SPRI_5889
SRW: TUE45_02094(mshC_2)
SLE: sle_54540(sle_54540)
SRN: A4G23_00897(mshC)
SNR: SNOUR_32045(mshC)
STRD: NI25_30875
SMAL: SMALA_1465
SLAU: SLA_1199
SALJ: SMD11_5351
SLX: SLAV_29190(mshC)
SFK: KY5_1496c
KSK: KSE_66390(mshC)
CMI: CMM_1694(cysS2)
CMS: CMS1669(cysS)
CMC: CMN_01673(mshC)
ART: Arth_2168
ARR: ARUE_c23240(cysS2)
ARM: ART_0678
ARN: CGK93_12595(mshC)
ARX: ARZXY2_1811(mshC)
ARTH: C3B78_10195(mshC)
AAU: AAur_2169
ACH: Achl_1911
AAI: AARI_16310(cysS)
KRH: KRH_13920(mshC)
RDN: HMPREF0733_11956(mshC)
BCV: Bcav_2247
JDE: Jden_1195
LMOI: VV02_16035
XCE: Xcel_1479
IDO: I598_0779(mshC)
CFL: Cfla_2010
CFI: Celf_1780
SERJ: SGUI_0324
BLIN: BLSMQ_2091
DCO: SAMEA4475696_1724(mshC)
PFR: PFREUD_14130(cysS1)
PFRE: RM25_1334
PPC: HMPREF9154_2051(mshC)
MPH: MLP_17040(mshC)
NCA: Noca_2628
NDK: I601_3334(mshC_1)
KFL: Kfla_3804
PSIM: KR76_13260
MGG: MPLG2_1802(mshC)
TFU: Tfu_1832
NDA: Ndas_1544
NAL: B005_4133(mshC)
TCU: Tcur_2287
SRO: Sros_5930
FAL: FRAAL2858(cysS2)
ACE: Acel_1172
NML: Namu_2780
GOB: Gobs_2668
BSD: BLASA_2691(cysS2)
MMAR: MODMU_2589(cysS)
KRA: Krad_1836
SEN: SACE_2226(cysS2)
AMD: AMED_4930(cysS)
AMN: RAM_25095
AMM: AMES_4871(cysS)
AMZ: B737_4871(cysS)
AOI: AORI_3794(cysS)
AJA: AJAP_20185(mshC)
AMQ: AMETH_3568(cysS)
AMYC: CU254_18120(mshC)
PDX: Psed_3330
PSEA: WY02_02660
PSEE: FRP1_12340
PSEH: XF36_10280
AMI: Amir_2201
SESP: BN6_31410(mshC)
KAL: KALB_3015
ACTI: UA75_14180
ACAD: UA74_14095
AHG: AHOG_13260(mshC)
SAQ: Sare_2322
MIL: ML5_5478
AMS: AMIS_53910(mshC)
ASE: ACPL_5266(mshC)
ACTN: L083_5113(cysS)
AFS: AFR_26630
ACTS: ACWT_5136
CAI: Caci_5399
SNA: Snas_4502
TBI: Tbis_1932
AFO: Afer_1230
AYM: YM304_19700(mshC)
RCA: Rcas_4315
CAU: Caur_0747
CAG: Cagg_2860
HAU: Haur_4263
TRO: trd_1909(cysS)
STI: Sthe_3518
TTR: Tter_1206
 » show all
Taxonomy
Reference
1  [PMID:17848100]
  Authors
Fan F, Luxenburger A, Painter GF, Blanchard JS
  Title
Steady-state and pre-steady-state kinetic analysis of Mycobacterium smegmatis cysteine ligase (MshC).
  Journal
Biochemistry 46:11421-9 (2007)
DOI:10.1021/bi7011492
  Sequence
Reference
2  [PMID:16908186]
  Authors
Gutierrez-Lugo MT, Newton GL, Fahey RC, Bewley CA
  Title
Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis.
  Journal
Protein Expr Purif 50:128-36 (2006)
DOI:10.1016/j.pep.2006.07.005
Reference
3  [PMID:19053270]
  Authors
Tremblay LW, Fan F, Vetting MW, Blanchard JS
  Title
The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway.
  Journal
Biochemistry 47:13326-35 (2008)
DOI:10.1021/bi801708f
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.13
IUBMB Enzyme Nomenclature: 6.3.1.13
ExPASy - ENZYME nomenclature database: 6.3.1.13
BRENDA, the Enzyme Database: 6.3.1.13

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