KEGG   ENZYME: 6.3.1.8Help
Entry
EC 6.3.1.8                  Enzyme                                 

Name
glutathionylspermidine synthase;
glutathione:spermidine ligase (ADP-forming)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy
Sysname
gamma-L-glutamyl-L-cysteinyl-glycine:spermidine ligase (ADP-forming) [spermidine is numbered so that atom N-1 is in the amino group of the aminopropyl part of the molecule]
Reaction(IUBMB)
glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate [RN:R01917]
Reaction(KEGG)
Substrate
glutathione [CPD:C00051];
spermidine [CPD:C00315];
ATP [CPD:C00002]
Product
glutathionylspermidine [CPD:C05730];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
Requires magnesium ions. Involved in the synthesis of trypanothione in trypanosomatids. The enzyme from Escherichia coli is bifunctional and also catalyses the glutathionylspermidine amidase (EC 3.5.1.78) reaction, resulting in a net hydrolysis of ATP.
History
EC 6.3.1.8 created 1999
Pathway
ec00480  Glutathione metabolism
ec01100  Metabolic pathways
Orthology
K01460  glutathionylspermidine amidase/synthetase
K01917  glutathionylspermidine synthase
Genes
TCR: 508479.110
LIF: LINJ_25_2500(GSP)
LDO: LDBPK_252500
LMI: LMXM_25_2380
ECO: b2988(gss)
ECJ: JW2956(gss)
ECD: ECDH10B_3165(gss)
EBW: BWG_2706(gsp)
ECOK: ECMDS42_2466(gss)
ECE: Z4342(gsp)
ECS: ECs3873
ECF: ECH74115_4300(gsp)
ETW: ECSP_3967(gsp)
EOJ: ECO26_4096(gss)
EOI: ECO111_3818(gss)
EOH: ECO103_3672(gss)
ECOO: ECRM13514_3891(gsp)
ECOH: ECRM13516_3759(gsp)
ECG: E2348C_3275(gsp)
EOK: G2583_3714(gsp)
ESO: O3O_21670
ESM: O3M_04015
ESL: O3K_03980
ECW: EcE24377A_3455(gsp)
ELH: ETEC_3262
ECC: c3725(gsp)
ECP: ECP_3073
ECI: UTI89_C3410(gsp)
ECV: APECO1_3433(gsp)
ECX: EcHS_A3168(gsp)
ECM: EcSMS35_3274(gsp)
ECY: ECSE_3273
ECR: ECIAI1_3137(gss)
ECQ: ECED1_3638(gss)
ECK: EC55989_3404(gss)
ECT: ECIAI39_3484(gss)
EOC: CE10_3521(gss)
EUM: ECUMN_3472(gss)
ECZ: ECS88_3370(gss)
ELO: EC042_3278(gsp)
ESE: ECSF_2819
EBR: ECB_02864(gss)
EBD: ECBD_0750
EKF: KO11_07735(gsp)
EAB: ECABU_c33940(gss)
EDJ: ECDH1ME8569_2888(gsp)
EIH: ECOK1_3407(gsp)
ENA: ECNA114_3071(gsp)
ELW: ECW_m3262(gsp)
ELL: WFL_15935(gsp)
ELC: i14_3416(gsp)
ELD: i02_3416(gsp)
EBL: ECD_02864(gss)
EBE: B21_02813(gsp)
ELF: LF82_0937(gsp)
ECOI: ECOPMV1_03293(gsp_1)
ECOJ: P423_16880
ECOS: EC958_3382
EFE: EFER_2930(gss)
EAL: EAKF1_ch2960(gsp)
STY: STY3310(gsp)
STT: t3060(gsp)
STM: STM3139(gsp)
SEO: STM14_3802(gsp)
SEY: SL1344_3113(gsp)
SEJ: STMUK_3127(gsp)
SEB: STM474_3289(gsp)
SEF: UMN798_3414(gsp)
SENR: STMDT2_30331(gsp)
SEND: DT104_31351(gsp)
SENI: CY43_16365
SPT: SPA3007(gsp)
SEK: SSPA2805
SEI: SPC_3208(gsp)
SEC: SCH_3080(gsp)
SHB: SU5_03637
SENS: Q786_15245
SED: SeD_A3486
SEL: SPUL_3145(gsp)
SEGA: SPUCDC_3131(gsp)
SET: SEN2982(gsp)
SENA: AU38_15165
SENO: AU37_15170
SENV: AU39_15170
SENQ: AU40_17095
SENL: IY59_15770
SEEP: I137_15025
SENB: BN855_32150(gsp)
SENE: IA1_15160
SBG: SBG_2738(gsp)
SBZ: A464_3170
SFL: SF3035(gsp)
SFX: S3236(gsp)
SFV: SFV_3041(gsp)
SFE: SFxv_3331(gsp)
SFN: SFy_4331
SFS: SFyv_4411
SFT: NCTC1_03289(gsp_1)
SSN: SSON_3133(gsp)
SBO: SBO_2875(gsp)
SBC: SbBS512_E3415(gsp)
SDY: SDY_3085(gsp)
ENC: ECL_04317
ECLO: ENC_32350
EEC: EcWSU1_03782(gsp)
ECLX: LI66_18940
ECLY: LI62_20885
ECLZ: LI64_17800
KPN: KPN_03416(gss)
KPU: KP1_4691(gss)
KPP: A79E_0706
KPE: KPK_0702(gsp)
KPJ: N559_0765
KPX: PMK1_00892(gsp_1)
KPNU: LI86_03705
KPNK: BN49_0688(gsp)
KVA: Kvar_0675
KOX: KOX_02835
KOE: A225_5016
EAE: EAE_03450
EAR: CCG33297
CKO: CKO_04379
CRO: ROD_35371(gsp)
CAMA: F384_25290
EBF: D782_0692
TPTY: NCTC11468_01666(gsp_2)
PMR: PMI0183(gsp)
PMIB: BB2000_0343(gsp)
MMK: MU9_3160
ETR: ETAE_2690
ETE: ETEE_0790(gsp)
LRI: NCTC12151_03225(gsp_2)
HIU: HIB_02850
MSU: MS0671(gsp)
ASU: Asuc_0804
AAT: D11S_0204
AAN: D7S_01327
AACN: AANUM_0246
PHA: PSHAa2455(gssA)
PTN: PTRA_a2949(gsp)
PART: PARC_a0816(gsp)
PTU: PTUN_a3222(gsp)
PNG: PNIG_a3134(gsp)
GNI: GNIT_0241(gsp)
GPS: C427_5446(gsp)
PIN: Ping_1235
SAGA: M5M_11030
TIG: THII_3705
RRF: F11_19045
 » show all
Taxonomy
Reference
1  [PMID:1304372]
  Authors
Smith K, Nadeau K, Bradley M, Walsh C, Fairlamb AH.
  Title
Purification of glutathionylspermidine and trypanothione synthetases from Crithidia fasciculata.
  Journal
Protein Sci 1:874-83 (1992)
DOI:10.1002/pro.5560010705
  Sequence
Reference
2  [PMID:7775463]
  Authors
Bollinger JM Jr, Kwon DS, Huisman GW, Kolter R, Walsh CT.
  Title
Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase.
  Journal
J Biol Chem 270:14031-41 (1995)
DOI:10.1074/jbc.270.23.14031
  Sequence
[eco:b2988]
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.8
IUBMB Enzyme Nomenclature: 6.3.1.8
ExPASy - ENZYME nomenclature database: 6.3.1.8
BRENDA, the Enzyme Database: 6.3.1.8
CAS: 9077-09-2

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