KEGG   ENZYME: 6.3.2.25Help
Entry
EC 6.3.2.25                 Enzyme                                 

Name
tubulin---tyrosine ligase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
alpha-tubulin:L-tyrosine ligase (ADP-forming)
Reaction(IUBMB)
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate [RN:R04730]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
detyrosinated alpha-tubulin [CPD:C05234];
L-tyrosine [CPD:C00082]
Product
alpha-tubulin [CPD:C01392];
ADP [CPD:C00008];
phosphate [CPD:C00009]
Comment
L-Tyrosine is linked via a peptide bond to the C-terminus of de-tyrosinated alpha-tubulin (des-Tyromega-alpha-tubulin). The enzyme is highly specific for alpha-tubulin and moderately specific for ATP and L-tyrosine. L-Phenylalanine and 3,4-dihydroxy-L-phenylalanine are transferred but with higher Km values.
History
EC 6.3.2.25 created 1999
Orthology
K06047  tubulin---tyrosine ligase
Genes
HSA: 150465(TTL)
PTR: 470483(TTL)
PPS: 100969421(TTL)
GGO: 101139501(TTL)
PON: 100450789(TTL)
NLE: 100599199(TTL)
MCC: 699075(TTL)
MCF: 102146185(TTL)
CSAB: 103241269(TTL)
RRO: 104680394(TTL)
RBB: 108518062(TTL)
CJC: 100404587(TTL)
SBQ: 101050847(TTL) 104652891
MMU: 69737(Ttl)
RNO: 171572(Ttl)
CGE: 100755055(Ttl)
NGI: 103739890(Ttl)
HGL: 101701462(Ttl)
OCU: 100353592(TTL)
TUP: 102487597(TTL)
CFA: 483065(TTL)
AML: 100474988(TTL)
UMR: 103671867(TTL)
ORO: 101380303(TTL)
FCA: 102900797(TTL)
PTG: 102966737(TTL)
AJU: 106976025(TTL)
BTA: 514559(TTL)
BOM: 102280366(TTL)
BIU: 109566127(TTL)
PHD: 102317169(TTL)
CHX: 102180860(TTL)
OAS: 101107272(TTL)
SSC: 445530(TTL)
CFR: 102509422(TTL)
CDK: 105093106(TTL)
BACU: 103018295(TTL)
LVE: 103087944(TTL)
OOR: 101270645(TTL)
ECB: 100052052(TTL)
EPZ: 103559679(TTL)
EAI: 106838551(TTL)
MYB: 102252588(TTL)
MYD: 102760056(TTL)
HAI: 109393550(TTL)
RSS: 109435864(TTL)
PALE: 102891762(TTL)
LAV: 100675880(TTL)
TMU: 101357599
MDO: 100031152(TTL)
SHR: 100930667(TTL)
OAA: 100089481
GGA: 416711(TTL)
MGP: 100540631(TTL)
CJO: 107310907(TTL)
APLA: 101798438(TTL)
ACYG: 106044757(TTL)
TGU: 100218069(TTL)
GFR: 102044350(TTL)
FAB: 101817591(TTL)
PHI: 102110480(TTL)
PMAJ: 107202369(TTL)
CCAE: 111926609(TTL)
CCW: 104696672
FPG: 101921873(TTL)
FCH: 102046034(TTL)
CLV: 102097117(TTL)
EGZ: 104128802(TTL)
AAM: 106494133(TTL)
ASN: 102387034(TTL)
AMJ: 102565195(TTL)
PSS: 102459357(TTL)
CMY: 102931299(TTL)
CPIC: 101933325(TTL)
ACS: 100561131(ttl)
PVT: 110087024(TTL)
PBI: 103067995(TTL)
GJA: 107114599(TTL)
XLA: 108716622(ttl.L)
XTR: 100135000(ttl)
NPR: 108801118(TTL)
DRE: 767687(ttl)
SGH: 107577318 107582496(ttl)
IPU: 108263154(ttl)
AMEX: 103045419(ttl)
TRU: 101075885(ttl)
LCO: 104921860(ttl)
NCC: 104949570(ttl)
MZE: 101486420(ttl)
OLA: 101168672(ttl)
XMA: 102227655(ttl)
PRET: 103477148(ttl)
NFU: 107375537(ttl)
KMR: 108247179(ttl)
CSEM: 103387495(ttl)
LCF: 108886280(ttl)
SDU: 111220626(ttl)
HCQ: 109523162(ttl)
BPEC: 110168527(ttl)
MALB: 109962255(ttl)
ELS: 105029715(ttl)
SFM: 108925774(ttl)
LCM: 102348001(TTL)
CMK: 103178932(ttl)
SPU: 582881
APLC: 110983169
SKO: 100372948
CRG: 105332425
LAK: 106165091
EPA: 110249149
ADF: 107353067
VCN: VOLCADRAFT_105441(ttlD)
TCR: 507801.50
LFA: LFA_0156
 » show all
Taxonomy
Reference
1  [PMID:3821560]
  Authors
Wehland J, Schroder HC, Weber K.
  Title
Isolation and purification of tubulin tyrosine ligase.
  Journal
Methods Enzymol 134:170-9 (1986)
Reference
2  [PMID:7510228]
  Authors
Rudiger M, Wehland J, Weber K.
  Title
The carboxy-terminal peptide of detyrosinated alpha tubulin provides a minimal system to study the substrate specificity of tubulin-tyrosine ligase.
  Journal
Eur J Biochem 220:309-20 (1994)
DOI:10.1111/j.1432-1033.1994.tb18627.x
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.25
IUBMB Enzyme Nomenclature: 6.3.2.25
ExPASy - ENZYME nomenclature database: 6.3.2.25
BRENDA, the Enzyme Database: 6.3.2.25
CAS: 60321-03-1

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