KEGG   ENZYME: 6.3.2.26Help
Entry
EC 6.3.2.26                 Enzyme                                 

Name
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase;
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-D-valine synthetase;
ACV synthetase;
L-alpha-aminoadipyl-cysteinyl-valine synthetase
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-2-aminohexanedioate:L-cysteine:L-valine ligase (AMP-forming, valine-inverting)
Reaction(IUBMB)
3 ATP + L-2-aminohexanedioate + L-cysteine + L-valine + H2O = 3 AMP + 3 diphosphate + N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [RN:R04870]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
L-2-aminohexanedioate [CPD:C00956];
L-cysteine [CPD:C00097];
L-valine [CPD:C00183];
H2O [CPD:C00001]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
N-[L-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine [CPD:C05556]
Comment
Requires Mg2+. The enzyme contains 4'-phosphopantetheine, which may be involved in the mechanism of the reaction. Forms part of the penicillin biosynthesis pathway (for pathway, click here).
History
EC 6.3.2.26 created 2002
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K12743  N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase
Genes
FCD: 110861984
ANI: AN2621.2
AOR: AO090038000543
AFV: AFLA_070860
ABE: ARB_02226
TVE: TRV_00553
SCT: SCAT_5684(pcbAB)
SCY: SCATT_56840
SCLF: BB341_07790
AAB: A4R43_03220
Taxonomy
Reference
1  [PMID:11851475]
  Authors
Byford MF, Baldwin JE, Shiau CY, Schofield CJ.
  Title
The Mechanism of ACV Synthetase.
  Journal
Chem Rev 97:2631-2650 (1997)
DOI:10.1021/cr960018l
Reference
2  [PMID:9355751]
  Authors
Theilgaard HB, Kristiansen KN, Henriksen CM, Nielsen J.
  Title
Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.
  Journal
Biochem J 327 ( Pt 1):185-91 (1997)
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.26
IUBMB Enzyme Nomenclature: 6.3.2.26
ExPASy - ENZYME nomenclature database: 6.3.2.26
BRENDA, the Enzyme Database: 6.3.2.26
CAS: 57219-73-5

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