LOCUS       AF329888                2689 bp    DNA     circular VRL 23-MAR-2004
DEFINITION  Maize streak virus - E[Pat] isolate MSV-Pat, complete genome.
ACCESSION   AF329888
VERSION     AF329888.1
KEYWORDS    .
SOURCE      Maize streak virus - E[Pat]
  ORGANISM  Maize streak virus - E[Pat]
            Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota;
            Repensiviricetes; Geplafuvirales; Geminiviridae; Mastrevirus; Maize
            streak virus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Martin,D.P., Willment,J.A., Billharz,R., Velders,R., Odhiambo,B.,
            Njuguna,J., James,D. and Rybicki,E.P.
  TITLE     Sequence diversity and virulence in Zea mays of Maize streak virus
            isolates
  JOURNAL   Virology 288 (2), 247-255 (2001)
   PUBMED   11601896
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Martin,D.P., James,D. and Rybicki,E.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2000) Microbiology, University of Cape Town,
            Private Bag, Cape Town, WC 7000, South Africa
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Maize streak virus - E[Pat]"
                     /mol_type="genomic DNA"
                     /strain="MSV-E"
                     /isolate="MSV-Pat"
                     /db_xref="taxon:268331"
                     /country="South Africa"
                     /note="isolated in 1999 from severely infected Digitaria"
     misc_feature    join(2682..2689,1..2)
                     /note="conserved nonanucleotide at origin of (+) strand
                     replication"
     stem_loop       19..2664
     gene            150..455
                     /gene="V1"
     CDS             150..455
                     /gene="V1"
                     /note="MP; facilitates intercellular movement of viral
                     DNA"
                     /codon_start=1
                     /product="movement protein"
                     /protein_id="AAK73467.1"
                     /translation="MDPQSAVYSLPRVPTAAPPNAGVPWSHVGEVAVLSFVALICIYL
                     LYLWVLRDLILVLKARRGRSTEELIFGSEAVDRRSPIPNTLEPTAPVHPGPFVPGSG"
     gene            466..1207
                     /gene="V2"
     CDS             466..1200
                     /gene="V2"
                     /note="nuclear localized ss/ds DNA binding protein"
                     /codon_start=1
                     /product="coat protein"
                     /protein_id="AAK73468.1"
                     /translation="MSTSKRKRADEVQWNKRSTKKKASAPPVKKTGGKADRPSLQIQT
                     LLHSGDTMITVPSGGGCDLISTYARGSDEGNRHTSETLTYKVGVDYHFVADAGACRYS
                     NRGTGVMWLVYDTTPGGNSPSTKDIFAYPDALVAWPTTWKVSRELCHRFVVKRRWLFT
                     METDGRIGSDIPPTNQSWPPCKRNIDFHKFTSGLGVRTQWKNVTDGGVGAIQRGALYM
                     VIAPGNGVTFTAHGQTRLYFKSVGNQ"
     regulatory      1203..1207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="V2"
     gene            complement(1217..2520)
                     /gene="C1/C2"
     regulatory      complement(1217..1221)
                     /regulatory_class="polyA_signal_sequence"
                     /gene="C1/C2"
     primer_bind     1274..1349
                     /note="origin of (-) strand replication"
     CDS             complement(join(1364..1789,1882..2520))
                     /gene="C1/C2"
                     /note="replication initiator/associated protein; required
                     for initiation and termination of viral DNA replication"
                     /codon_start=1
                     /product="Rep"
                     /protein_id="AAK73470.1"
                     /translation="MASSSSNRSFLHRNANTFLTYPHCPENPEIISQKLWDLVARWNP
                     LYIVCAREAHRDGNMHLHALLQTDKPVRTTDARIFDIEGFHPNIQSAKSVNKVRDYIL
                     KEPLAVFERGTFIPRKSCFQGNTPPFPKKNPNKDEIMAHIISHATSKQEYLCLVRKEF
                     PYDWATKLQYFEYSANKLFPDIQEEFISPHPPSSPDLLCNESIKDWLQPNIYQPADEG
                     SRKQSLYIVGPTRTGKSTWARSLGLHNYWQNNVDWSSYNEDAIYNIVDDIPFKYCPCW
                     KQLVGCQKEFVVNPKYGKKKKVQMKSKPTIILANSDEDWMKEMTPGQLEYFEANCMIY
                     VMSPGEKWYSPPQLPPTEEV"
     gene            complement(1705..2520)
                     /gene="C1"
     CDS             complement(1705..2520)
                     /gene="C1"
                     /note="replication initiator/associated protein ORFA;
                     possibly involved in cell cycle regulation"
                     /codon_start=1
                     /product="RepA"
                     /protein_id="AAK73469.1"
                     /translation="MASSSSNRSFLHRNANTFLTYPHCPENPEIISQKLWDLVARWNP
                     LYIVCAREAHRDGNMHLHALLQTDKPVRTTDARIFDIEGFHPNIQSAKSVNKVRDYIL
                     KEPLAVFERGTFIPRKSCFQGNTPPFPKKNPNKDEIMAHIISHATSKQEYLCLVRKEF
                     PYDWATKLQYFEYSANKLFPDIQEEFISPHPPSSPDLLCNESIKDWLQPNIYQVSPEA
                     YMLLQPTCYTVDEAISDLTWMDNLSSQQMKDQESRASTSSVQQGQGNLLGPEV"
     repeat_region   2590..2597
                     /note="iteron"
     regulatory      2633..2661
                     /regulatory_class="GC_signal"
ORIGIN      
        1 accgcgcctt ctttcctgcg agggcccgca gggaccgagc gtctttgatt taaaggtttg
       61 atctgctttg gtggattttt ccaaagcagc cgtgtttaaa gaaaccggtc ccgtctcact
      121 ataaattgtc taacaagtgc gattcattca tggatcccca gagtgctgtt tactctttgc
      181 cgcgggtacc cacagcagct ccgcccaacg caggagtgcc gtggagtcac gtcggcgagg
      241 tagctgtttt gagctttgtt gctttgattt gcatttactt gctttacctt tgggtgctga
      301 gagatcttat cttagtcttg aaggcaagac gcgggaggtc cacggaggag ctgatatttg
      361 gatctgaagc tgtggatagg aggagtccta tccctaatac tttggaacca acagctccag
      421 tgcatcccgg accatttgtt cctggttcgg gataagcagt gagccatgtc gacgtccaag
      481 aggaagcgtg ccgacgaggt gcagtggaat aagaggtcca ccaagaagaa agcttctgca
      541 ccgccggtga agaagactgg gggtaaggct gacaggcctt ccctccagat acagactcta
      601 ctccactcgg gggacaccat gataaccgta ccgtcaggtg ggggctgtga cctcatcagt
      661 acatatgccc gaggatccga tgagggcaac cgtcacacca gcgaaaccct aacgtacaaa
      721 gttggggtcg actaccactt cgtcgccgac gcaggagcct gcagatactc caaccgcgga
      781 acaggtgtga tgtggctggt gtacgacact acccccggcg gaaactcacc gtccacgaag
      841 gacatcttcg cctatccgga tgcactagta gcttggccga cgacttggaa agttagtcgg
      901 gagttgtgtc accgcttcgt ggtgaagcgg cgatggctct tcacaatgga gaccgacggt
      961 cgaattggtt cggatatccc tccgaccaat cagagttggc cgccatgtaa gcggaacata
     1021 gacttccaca agttcactag cgggttggga gtgaggacgc agtggaagaa tgtaacagac
     1081 ggtggggtcg gtgcgataca gagaggggct ctgtatatgg tcattgcccc tggcaatggt
     1141 gtaacgttta ctgcccatgg gcagacacgt ctgtacttca agagtgtggg caaccagtaa
     1201 tgaataaaac cccgttttat tatacctgat gaatgccgaa agcttacatt aatatgtcgt
     1261 gcgatggcac gaaaacacgc tatcaataca ggggggtcgg ggcaaagcag gcggctatag
     1321 ggcgcccccg gggcaaaaca ttaaaaaaac accgatatat gaattacact tcctccgtag
     1381 gaggaagctg agggggagaa taccatttct cccccggcga cataacataa atcatgcagt
     1441 ttgcttcgaa atactccagt tgccctggag tcatttcctt catccagtct tcatccgagt
     1501 tggcgaggat tattgtaggt ttagacttca tctgaacctt cttcttcttg ccatatttag
     1561 gatttacaac gaactccttt tggcagccaa ctaactgttt ccaacaagga caatatttaa
     1621 aagggatatc atctacgatg ttatagattg cgtcttcgtt gtaggaagac caatcaacat
     1681 tattttgcca gtaattatga agccctaaac ttctggccca agtagatttc cctgtccttg
     1741 ttggaccgac gatgtagagg ctctgctttc ttgatccttc atctgctggc tggataagtt
     1801 atccatccat gttagatctg aaattgcttc atcgacggta taacaggtag gttgaagaag
     1861 catgtatgct tcgggactga cctgatagat gttaggctgg agccaatctt tgattgactc
     1921 attacaaagc agatcaggtg aagagggtgg atgaggactg atgaactctt cctgaatgtc
     1981 aggaaagagt ttgtttgcag agtattcgaa atactgcaat ttggtggccc agtcgtaagg
     2041 gaattccttc cgaaccaagc agaggtactc ttgcttggaa gtggcgtgag aaataatgtg
     2101 tgccattatt tcatctttgt ttgggttttt tttcgggaag gggggagtat ttccttggaa
     2161 gcatgacttc cgaggaatga aagtacctct ctcaaatact gccagtggtt ccttgagaat
     2221 gtagtcgcgt accttgttta cggacttagc actctgaatg tttgggtgga atccttctat
     2281 gtcgaaaatc ctggcatcgg tcgtcctcac tggtttatcc gtctgtagta atgcatgtaa
     2341 gtgcatattt ccatcacgat gtgcctctcg ggcacatacg atgtacagag gattccaacg
     2401 agcgacgaga tcccatagtt tctgactgat gatttctgga ttttctggac agtgtggata
     2461 tgtgaggaat gtgtttgcat tcctgtgtaa gaacgaacgg ttggatgagg aggaggccat
     2521 atccgacgac ggaggctagt tgctagctca aggcagaaac gggagctccc aaactctatg
     2581 taaccgggtg cgccttcgaa atccgccgcc cctatttata gacgatttag ctgggccgga
     2641 acgggccagg ccggccggcc cagcaggaaa gaaggcgcgc actaatatt
//