KEGG   PATHWAY: map03030Help
Entry
map03030                    Pathway                                

Name
DNA replication
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
09120 Genetic Information Processing; 09124 Replication and repair
BRITE hierarchy
Pathway map
map03030  DNA replication
map03030

Ortholog table
Module
M00260  DNA polymerase III complex, bacteria [PATH:map03030]
M00261  DNA polymerase alpha / primase complex [PATH:map03030]
M00262  DNA polymerase delta complex [PATH:map03030]
M00263  DNA polymerase epsilon complex [PATH:map03030]
M00285  MCM complex [PATH:map03030]
M00288  RPA complex [PATH:map03030]
M00289  RF-C complex [PATH:map03030]
Disease
H00290  Aicardi-Goutieres syndrome (AGS)
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
ko03030   

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