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Entry
ecf02060                    Pathway                                

Name
Phosphotransferase system (PTS) - Escherichia coli O157:H7 EC4115 (EHEC)
Description
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the phosphoryl group is transferred to histidine or cysteine residues of EIIB. The sugar is transported through the membrane-bound EIIC and is phosphorylated by the appropriate sugar-specific EIIB.
Class
Environmental Information Processing; Membrane transport
BRITE hierarchy
Pathway map
ecf02060  Phosphotransferase system (PTS)
ecf02060

Ortholog table
Module
ecf_M00265  PTS system, glucose-specific II component [PATH:ecf02060]
ecf_M00266  PTS system, maltose/glucose-specific II component [PATH:ecf02060]
ecf_M00267  PTS system, N-acetylglucosamine-specific II component [PATH:ecf02060]
ecf_M00268  PTS system, alpha-glucoside-specific II component [PATH:ecf02060]
ecf_M00270  PTS system, trehalose-specific II component [PATH:ecf02060]
ecf_M00272  PTS system, beta-glucoside (arbutin/salicin/cellobiose)-specific II component [PATH:ecf02060]
ecf_M00273  PTS system, fructose-specific II component [PATH:ecf02060]
ecf_M00274  PTS system, mannitol-specific II component [PATH:ecf02060]
ecf_M00275  PTS system, cellobiose-specific II component [PATH:ecf02060]
ecf_M00276  PTS system, mannose-specific II component [PATH:ecf02060]
ecf_M00277  PTS system, N-acetylgalactosamine-specific II component [PATH:ecf02060]
ecf_M00279  PTS system, galactitol-specific II component [PATH:ecf02060]
ecf_M00283  PTS system, ascorbate-specific II component [PATH:ecf02060]
ecf_M00303  PTS system, N-acetylmuramic acid-specific II component [PATH:ecf02060]
Other DBs
BSID: 75408
GO: 0009401
TC: 4.A
Organism
Escherichia coli O157:H7 EC4115 (EHEC) [GN:ecf]
Gene
ECH74115_3647  ptsI; phosphoenolpyruvate-protein phosphotransferase [KO:K08483] [EC:2.7.3.9]
ECH74115_3615  fryA; multiphosphoryl transfer protein 1 [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.-]
ECH74115_5408  ptsA; multi-phosphoryl transfer protein 2 [KO:K08483 K11189 K11201] [EC:2.7.3.9 2.7.1.-]
ECH74115_3646  ptsH; phosphocarrier protein PtsH [KO:K02784]
ECH74115_3648  crr; PTS system, glucose-specific IIA component [KO:K02777] [EC:2.7.1.-]
ECH74115_1480  ptsG; PTS system, glucose-specific IIBC component [KO:K02778 K02779] [EC:2.7.1.199]
ECH74115_0772  nagE; PTS system, N-acetylglucosamine-specific IICBA component [KO:K02802 K02803 K02804] [EC:2.7.1.193 2.7.1.193]
ECH74115_2333  malX; PTS system, maltose and glucose-specific IIBC component [KO:K02790 K02791] [EC:2.7.1.208 2.7.1.199]
ECH74115_3966  ascF; PTS system, arbutin-, cellobiose-, and salicin-specific, EIIBC component [KO:K02752 K02753] [EC:2.7.1.-]
ECH74115_5114  PTS system, alpha-glucoside-specific IIBC component [KO:K02749 K02750] [EC:2.7.1.- 2.7.1.208]
ECH74115_5762  treB; PTS system, trehalose-specific IIBC component [KO:K02818 K02819] [EC:2.7.1.201]
ECH74115_3659  murP; PTS system, N-acetylmuramic acid-specific EIIBC component [KO:K11191 K11192] [EC:2.7.1.192]
ECH74115_4969  mtlA; PTS system, mannitol-specific IIABC component [KO:K02798 K02799 K02800] [EC:2.7.1.197 2.7.1.197]
ECH74115_4235  cmtB; mannitol-specific cryptic phosphotransferase enzyme IIA component [KO:K02798] [EC:2.7.1.197]
ECH74115_4234  PTS system, mannitol-specific cryptic EIICB component [KO:K02799 K02800] [EC:2.7.1.197]
ECH74115_2454  chbA; PTS system, N,N'-diacetylchitobiose-specific EIIA component [KO:K02759] [EC:2.7.1.205 2.7.1.196]
ECH74115_2456  chbB; PTS system, N,N'-diacetylchitobiose-specific IIB component [KO:K02760] [EC:2.7.1.205 2.7.1.196]
ECH74115_2455  chbC; PTS system, N,N'-diacetylchitobiose-specific IIC component [KO:K02761]
ECH74115_2546  manX; PTS system, mannose-specific IIAB component [KO:K02793 K02794] [EC:2.7.1.191 2.7.1.191]
ECH74115_2547  manY; PTS system, mannose-specific IIC component [KO:K02795]
ECH74115_2548  manZ; PTS system, mannose-specific IID component [KO:K02796]
ECH74115_5497  PTS system, mannose/fructose/sorbose family, IIA component [KO:K02812] [EC:2.7.1.206]
ECH74115_5495  PTS system, mannose/fructose/sorbose family, IIC component [KO:K02814]
ECH74115_5494  PTS system, mannose/fructose/sorbose family, IID component [KO:K02815]
ECH74115_4450  agaF; PTS system, mannose/sorbose-specific, IIA component [KO:K02744] [EC:2.7.1.-]
ECH74115_4447  agaV; PTS system, N-acetylgalactosamine-specific, IIB component [KO:K02745] [EC:2.7.1.-]
ECH74115_4448  agaW; PTS system, N-acetylgalactosamine-specific, IIC component [KO:K02746]
ECH74115_4449  agaE; PTS system N-acetylgalactosamine-specific, IID component [KO:K02747]
ECH74115_4455  agaB; PTS system, D galactosamine-specific, IIB component [KO:K10984] [EC:2.7.1.-]
ECH74115_4457  agaD; PTS system, D-galactosamine-specific, IID component [KO:K10986]
ECH74115_3954  srlB; PTS system, glucitol/sorbitol-specific, IIA component [KO:K02781] [EC:2.7.1.198]
ECH74115_4823  PEP-dependent sugar transporting PTS family, IIA component [KO:K02773] [EC:2.7.1.200]
ECH74115_3073  gatA; PTS system, galactitol-specific IIB component [KO:K02773] [EC:2.7.1.200]
ECH74115_4824  PEP-dependent sugar transporting PTS family, IIB component [KO:K02774] [EC:2.7.1.200]
ECH74115_3072  gatB; PTS system, galactitol-specific IIB component [KO:K02774] [EC:2.7.1.200]
ECH74115_4825  PEP-dependent sugar transporting PTS family, IIC component [KO:K02775]
ECH74115_3071  gatC; PTS system, galactitol-specific IIC component [KO:K02775]
ECH74115_5711  ulaC; ascorbate-specific phosphotransferase enzyme IIA component [KO:K02821] [EC:2.7.1.194]
ECH74115_5710  ulaB; ascorbate-specific phosphotransferase enzyme IIB component [KO:K02822] [EC:2.7.1.194]
ECH74115_5709  ulaA; ascorbate-specific permease IIC component [KO:K03475]
ECH74115_1016  ascorbate-specific PTS system enzyme IIC [KO:K03475]
ECH74115_3305  fruB; PTS system fructose-specific phosphotransferase enzyme IIA component [KO:K02768 K11183] [EC:2.7.1.202]
ECH74115_3303  fruA; PTS system, fructose-specific, IIBC component [KO:K02769 K02770] [EC:2.7.1.202]
ECH74115_4095  ptsP; phosphoenolpyruvate-protein phosphotransferase [KO:K08484] [EC:2.7.3.9]
ECH74115_4528  ptsO; phosphocarrier protein NPr [KO:K08485]
ECH74115_4526  ptsN; PTS IIA-like nitrogen-regulatory protein PtsN [KO:K02806] [EC:2.7.1.-]
Compound
C00022  Pyruvate
C00031  D-Glucose
C00072  Ascorbate
C00074  Phosphoenolpyruvate
C00089  Sucrose
C00092  D-Glucose 6-phosphate
C00095  D-Fructose
C00124  D-Galactose
C00140  N-Acetyl-D-glucosamine
C00159  D-Mannose
C00185  Cellobiose
C00208  Maltose
C00243  Lactose
C00275  D-Mannose 6-phosphate
C00329  D-Glucosamine
C00352  D-Glucosamine 6-phosphate
C00357  N-Acetyl-D-glucosamine 6-phosphate
C00392  Mannitol
C00644  D-Mannitol 1-phosphate
C00689  alpha,alpha'-Trehalose 6-phosphate
C00697  Nitrogen
C00794  D-Sorbitol
C01083  alpha,alpha-Trehalose
C01094  D-Fructose 1-phosphate
C01096  Sorbitol 6-phosphate
C01113  D-Galactose 6-phosphate
C01132  N-Acetyl-D-galactosamine
C01451  Salicin
C01452  Sorbose
C01674  Chitobiose
C01697  Galactitol
C01798  D-Glucoside
C02262  D-Galactosamine
C02713  N-Acetylmuramate
C02888  Sorbose 1-phosphate
C02995  Maltose 6'-phosphate
C03752  2-Amino-2-deoxy-D-gluconate
C04534  6-Phospho-beta-D-glucosyl-(1,4)-D-glucose
C05396  Lactose 6'-phosphate
C06186  Arbutin
C06187  Arbutin 6-phosphate
C06188  Salicin 6-phosphate
C06311  Galactitol 1-phosphate
C06376  N-Acetyl-D-galactosamine 6-phosphate
C06377  D-Galactosamine 6-phosphate
C11544  2-O-(alpha-D-Mannosyl)-D-glycerate
C16186  L-Ascorbate 6-phosphate
C16488  Fructoselysine
C16489  Fructoselysine 6-phosphate
C16688  Sucrose 6-phosphate
C16698  N-Acetylmuramic acid 6-phosphate
C16699  2-O-(6-Phospho-alpha-mannosyl)-D-glycerate
C20589  D-Glucosaminate-6-phosphate
C20978  Glucoselysine
C20979  Glucoselysine-6-phosphate
C21152  N,N'-Diacetylchitobiose 6'-phosphate
Reference
  Authors
Darbon E, Galinier A, Le Coq D, Deutscher J.
  Title
Phosphotransfer functions mutated Bacillus subtilis HPr-like protein Crh carrying a histidine in the active site.
  Journal
J Mol Microbiol Biotechnol 3:439-44 (2001)
Reference
  Authors
Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C.
  Title
Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid.
  Journal
J Bacteriol 186:2385-92 (2004)
DOI:10.1128/JB.186.8.2385-2392.2004
Reference
PMID:2117666
  Authors
Martin-Verstraete I, Debarbouille M, Klier A, Rapoport G
  Title
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
  Journal
J Mol Biol 214:657-71 (1990)
DOI:10.1016/0022-2836(90)90284-S
Reference
  Authors
Kristich CJ, Glekas GD, Ordal GW.
  Title
The conserved cytoplasmic module of the transmembrane chemoreceptor McpC mediates carbohydrate chemotaxis in Bacillus subtilis.
  Journal
Mol Microbiol 47:1353-66 (2003)
DOI:10.1046/j.1365-2958.2003.03375.x
Reference
  Authors
Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO.
  Title
Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.
  Journal
J Biol Chem 279:5537-48 (2004)
DOI:10.1074/jbc.M310980200
Reference
  Authors
Keseler IM, Collado-Vides J, Gama-Castro S, Ingraham J, Paley S, Paulsen IT, Peralta-Gil M, Karp PD.
  Title
EcoCyc: a comprehensive database resource for Escherichia coli.
  Journal
Nucleic Acids Res 33:D334-7 (2005)
DOI:10.1093/nar/gki108
Reference
  Authors
Mukherjee A, Mammel MK, LeClerc JE, Cebula TA.
  Title
Altered utilization of N-acetyl-D-galactosamine by Escherichia coli O157:H7 from the 2006 spinach outbreak.
  Journal
J Bacteriol 190:1710-7 (2008)
DOI:10.1128/JB.01737-07
Reference
  Authors
Brinkkotter A, Kloss H, Alpert C, Lengeler JW.
  Title
Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli.
  Journal
Mol Microbiol 37:125-35 (2000)
DOI:10.1046/j.1365-2958.2000.01969.x
Reference
  Authors
Tchieu JH, Norris V, Edwards JS, Saier MH Jr.
  Title
The complete phosphotranferase system in Escherichia coli.
  Journal
J Mol Microbiol Biotechnol 3:329-46 (2001)
Reference
  Authors
Kotrba P, Inui M, Yukawa H.
  Title
Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism.
  Journal
J Biosci Bioeng 92:502-17 (2001)
DOI:10.1016/S1389-1723(01)80308-X
KO pathway
ko02060   

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