Entry
Name
Phosphotransferase system (PTS) - Photorhabdus asymbiotica
Description
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the phosphoryl group is transferred to histidine or cysteine residues of EIIB. The sugar is transported through the membrane-bound EIIC and is phosphorylated by the appropriate sugar-specific EIIB.
Class
Environmental Information Processing; Membrane transport
BRITE hierarchy
Pathway map
pay02060 Phosphotransferase system (PTS)
Ortholog table
Other DBs
Organism
Photorhabdus asymbiotica [GN:
pay ]
Gene
PAU_01403 ptsI; phosphoenolpyruvate-protein phosphotransferase (phosphotransferas system, enzyme i) [KO:K08483 ] [EC:2.7.3.9 ]
PAU_01404 ptsH; phosphocarrier protein hpr (histidine-containing protein) [KO:K02784 ]
PAU_01402 crr; glucose-specific phosphotransferase enzyme IIA component [KO:K02777 ] [EC:2.7.1.-]
PAU_00547 bglF; beta-glucoside-specific phosphotransferase system-dependent permease [KO:K02757 ] [EC:2.7.1.-]
PAU_00549 bglP; similar to beta-glucoside permease iiabc component [KO:K02757 ] [EC:2.7.1.-]
PAU_01357 treB; pts system, trehalose-specific iibc component (eiibc-tre) (trehalose permease iibc component) (phosphotransferase enzyme ii, bc component (eii-tre) [KO:K02819 ] [EC:2.7.1.201 ]
PAU_01786 celC; pts system, cellobiose-specific iia component (eiia-cel) (cellobiose permease iia component) (phosphotransferase enzyme ii, a component (eiii-cel) [KO:K02759 ] [EC:2.7.1.196 2.7.1.205 ]
PAU_01787 celB; phosphotransferase system cellobiose-specific component iic [KO:K02761 ]
PAU_01837 manX; pts system, mannose-specific iiab component (eiiab-man) (mannose permease iiab component) (phosphotransferase enzyme ii, ab component (eiii-man) [KO:K02794 ] [EC:2.7.1.191 ]
PAU_01836 manY; pts system, mannose-specific iic component (eiic-man) (mannose permease iic component) (phosphotransferase enzyme ii, c component (eii-p-man) [KO:K02795 ]
PAU_01835 manZ; pts system, mannose-specific iid component (eiid-man) (mannose permease iid component) (phosphotransferase enzyme ii, d component (eii-m-man) [KO:K02796 ]
PAU_00778 yadI; similar to putative pts system iia component yadi of escherichia coli [KO:K02744 ] [EC:2.7.1.-]
PAU_00775 agaV; pts system, n-acetylgalactosamine-specific iib component 2 (eiib-aga' (n-acetylgalactosamine-permease iib component 2) (phosphotransferas enzyme ii, b component 2) [KO:K02745 ] [EC:2.7.1.-]
PAU_00776 agaC; pts system, n-acetylgalactosamine-specific iic component 1 (eiic-aga (n-acetylgalactosamine-permease iic component 1) [KO:K02746 ]
PAU_00777 agaD; pts system, n-acetylgalactosamine-specific iid component (eiid-aga (n-acetylgalactosamine-permease iid component) (phosphotransferas enzyme ii, d component) [KO:K02747 ]
PAU_00580 ptsP; phosphoenolpyruvate-protein phosphotransferase pts (phosphotransferase system, enzyme i) (enzyme i-ntr) [KO:K08484 ] [EC:2.7.3.9 ]
PAU_03683 ptsO; phosphocarrier protein npr (nitrogen related hpr) [KO:K08485 ]
PAU_03681 ptsN; nitrogen regulatory iia protein (enzyme iia-ntr) (phosphotransferas enzyme ii, a component) [KO:K02806 ] [EC:2.7.1.-]
Compound
C00352 D-Glucosamine 6-phosphate
C00354 D-Fructose 1,6-bisphosphate
C00357 N-Acetyl-D-glucosamine 6-phosphate
C00689 alpha,alpha'-Trehalose 6-phosphate
C01113 D-Galactose 6-phosphate
C01132 N-Acetyl-D-galactosamine
C03752 2-Amino-2-deoxy-D-gluconate
C04534 6-Phospho-beta-D-glucosyl-(1,4)-D-glucose
C06376 N-Acetyl-D-galactosamine 6-phosphate
C06377 D-Galactosamine 6-phosphate
C11544 2-O-(alpha-D-Mannosyl)-D-glycerate
C16186 L-Ascorbate 6-phosphate
C16489 Fructoselysine 6-phosphate
C16698 N-Acetylmuramic acid 6-phosphate
C16699 2-O-(6-Phospho-alpha-mannosyl)-D-glycerate
C20589 D-Glucosaminate-6-phosphate
C20979 Glucoselysine-6-phosphate
C21152 N,N'-Diacetylchitobiose 6'-phosphate
Reference
Authors
Darbon E, Galinier A, Le Coq D, Deutscher J.
Title
Phosphotransfer functions mutated Bacillus subtilis HPr-like protein Crh carrying a histidine in the active site.
Journal
J Mol Microbiol Biotechnol 3:439-44 (2001)
Reference
Authors
Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C.
Title
Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid.
Journal
Reference
Authors
Martin-Verstraete I, Debarbouille M, Klier A, Rapoport G
Title
Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon.
Journal
Reference
Authors
Kristich CJ, Glekas GD, Ordal GW.
Title
The conserved cytoplasmic module of the transmembrane chemoreceptor McpC mediates carbohydrate chemotaxis in Bacillus subtilis.
Journal
Reference
Authors
Sampaio MM, Chevance F, Dippel R, Eppler T, Schlegel A, Boos W, Lu YJ, Rock CO.
Title
Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor.
Journal
Reference
Authors
Keseler IM, Collado-Vides J, Gama-Castro S, Ingraham J, Paley S, Paulsen IT, Peralta-Gil M, Karp PD.
Title
EcoCyc: a comprehensive database resource for Escherichia coli.
Journal
Reference
Authors
Mukherjee A, Mammel MK, LeClerc JE, Cebula TA.
Title
Altered utilization of N-acetyl-D-galactosamine by Escherichia coli O157:H7 from the 2006 spinach outbreak.
Journal
Reference
Authors
Brinkkotter A, Kloss H, Alpert C, Lengeler JW.
Title
Pathways for the utilization of N-acetyl-galactosamine and galactosamine in Escherichia coli.
Journal
Reference
Authors
Tchieu JH, Norris V, Edwards JS, Saier MH Jr.
Title
The complete phosphotranferase system in Escherichia coli.
Journal
J Mol Microbiol Biotechnol 3:329-46 (2001)
Reference
Authors
Kotrba P, Inui M, Yukawa H.
Title
Bacterial phosphotransferase system (PTS) in carbohydrate uptake and control of carbon metabolism.
Journal
Related pathway
pay00051 Fructose and mannose metabolism
KO pathway