KEGG PATHWAY: ehi00563

PATHWAY: ehi00563

Entry

ehi00563                    Pathway

Name

Glycosylphosphatidylinositol (GPI)-anchor biosynthesis - Entamoeba histolytica

Description

Cell surface proteins can be attached to the cell membrane via the glycolipid structure called glycosylphosphatidylinositol (GPI) anchor. Hundreds of GPI-anchored proteins have been identified in many eukaryotes ranging from protozoa and fungi to mammals. All protein-linked GPI anchors share a common core structure, characterized by the substructure Man (a1-4) GlcN (a1-6) myo-inositol-1P-lipid. Biosynthesis of GPI anchors proceeds in three stages: (i) preassembly of a GPI precursor in the ER membrane, (ii) attachment of the GPI to the C-terminus of a newly synthesized protein in the lumen of the ER, and (iii) lipid remodeling and/or carbohydrate side-chain modifications in the ER and the Golgi. Defects of GPI anchor biosynthesis gene result in a genetic disorder, paroxysmal nocturnal hemoglobinuria.

Class

Metabolism; Glycan biosynthesis and metabolism

Pathway map

ehi00563

Glycosylphosphatidylinositol (GPI)-anchor biosynthesis

Other DBs

GO:

0006506

Organism

Entamoeba histolytica [GN:ehi]

Gene

EHI_105870

9.t00084; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A [KO:K03857] [EC:2.4.1.198]

EHI_031520

80.t00011; phosphatidylinositol N-acetylglucosaminyltransferase subunit C [KO:K03859]

EHI_005920

83.t00006; hypothetical protein [KO:K03858]

EHI_126130

465.t00003; hypothetical protein [KO:K03861]

EHI_069120

32.t00039; N-acetylglucosaminyl transferase [KO:K03860]

EHI_015270

52.t00009; N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase [KO:K03434] [EC:3.5.1.89]

EHI_031230

63.t00007; hypothetical protein [KO:K05283] [EC:2.3.-.-]

EHI_029580

248.t00010; mannosyltransferase [KO:K05284] [EC:2.4.1.-]

EHI_103330

118.t00023; mannosyltransferase [KO:K05284] [EC:2.4.1.-]

EHI_130880

399.t00002; hypothetical protein [KO:K07541]

EHI_068330

61.t00010; hypothetical protein [KO:K07542] [EC:2.4.1.-]

EHI_107320

105.t00028; phosphatidylinositol-glycan biosynthesis class O protein [KO:K05288] [EC:2.7.-.-]

EHI_196400

299.t00007; hypothetical protein [KO:K05289]

EHI_005120

103.t00026; hypothetical protein [KO:K05291]

EHI_001830

92.t00008; hypothetical protein [KO:K05292]

EHI_126280

117.t00034; hypothetical protein [KO:K05293]

Compound

C00154

Palmitoyl-CoA

C00346

Ethanolamine phosphate

C00350

Phosphatidylethanolamine

C01194

1-Phosphatidyl-D-myo-inositol

G00143

G00144

G00145

G00146

G00148

G00149

G10610

UDP-N-acetyl-D-glucosamine

G10617

Dolichyl phosphate D-mannose

G12396

6-(alpha-D-glucosaminyl)-1D-myo-inositol

G13044

G13046

G13128

G13150

G13151

G13152

Reference

PMID:12655644

Authors

Eisenhaber B, Maurer-Stroh S, Novatchkova M, Schneider G, Eisenhaber F.

Title

Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins.

Journal

Bioessays 25:367-85 (2003)
DOI:10.1002/bies.10254

Reference

PMID:11102867

Authors

Kinoshita T, Inoue N.

Title

Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis.

Journal

Curr Opin Chem Biol 4:632-8 (2000)
DOI:10.1016/S1367-5931(00)00151-4

Reference

PMID:8253761

Authors

Fankhauser C, Homans SW, Thomas-Oates JE, McConville MJ, Desponds C, Conzelmann A, Ferguson MA.

Title

Structures of glycosylphosphatidylinositol membrane anchors from Saccharomyces cerevisiae.

Journal

J Biol Chem 268:26365-74 (1993)

Reference

PMID:14517336

Authors

Murakami Y, Siripanyapinyo U, Hong Y, Kang JY, Ishihara S, Nakakuma H, Maeda Y, Kinoshita T.

Title

PIG-W is critical for inositol acylation but not for flipping of glycosylphosphatidylinositol-anchor.

Journal

Mol Biol Cell 14:4285-95 (2003)
DOI:10.1091/mbc.E03-03-0193

Reference

PMID:10329735

Authors

Benachour A, Sipos G, Flury I, Reggiori F, Canivenc-Gansel E, Vionnet C, Conzelmann A, Benghezal M.

Title

Deletion of GPI7, a yeast gene required for addition of a side chain to the glycosylphosphatidylinositol (GPI) core structure, affects GPI protein transport, remodeling, and cell wall integrity.

Journal

J Biol Chem 274:15251-61 (1999)
DOI:10.1074/jbc.274.21.15251

Reference

PMID:14734546

Authors

Tanaka S, Maeda Y, Tashima Y, Kinoshita T.

Title

Inositol deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p.

Journal

J Biol Chem 279:14256-63 (2004)
DOI:10.1074/jbc.M313755200

Reference

PMID:12802054

Authors

Hong Y, Ohishi K, Kang JY, Tanaka S, Inoue N, Nishimura J, Maeda Y, Kinoshita T.

Title

Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.

Journal

Mol Biol Cell 14:1780-9 (2003)
DOI:10.1091/mbc.E02-12-0794

Reference

PMID:12807000

Authors

Tanaka S, Maeda Y, Kinoshita T.

Title

[Biosynthesis of GPI-anchored proteins]

Journal

Tanpakushitsu Kakusan Koso 48:990-6 (2003)

Reference

PMID:15623507

Authors

Kang JY, Hong Y, Ashida H, Shishioh N, Murakami Y, Morita YS, Maeda Y, Kinoshita T.

Title

PIG-V involved in transferring the second mannose in glycosylphosphatidylinositol.

Journal

J Biol Chem 280:9489-97 (2005)
DOI:10.1074/jbc.M413867200

Reference

PMID:15635094

Authors

Ashida H, Hong Y, Murakami Y, Shishioh N, Sugimoto N, Kim YU, Maeda Y, Kinoshita T.

Title

Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I.

Journal

Mol Biol Cell 16:1439-48 (2005)
DOI:10.1091/mbc.E04-09-0802

Reference

PMID:24364251

Authors

Fujita M

Title

[Biosynthesis and remodeling of GPI-anchored proteins].

Journal

Seikagaku 85:985-95 (2013)

Reference

PMID:25509331

Authors

Kinoshita T

Title

[Biochemistry of glycosylphosphatidylinositol (GPI) anchored proteins].

Journal

Seikagaku 86:626-36 (2014)

Reference

PMID:19837036

Authors

Fujita M, Maeda Y, Ra M, Yamaguchi Y, Taguchi R, Kinoshita T

Title

GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi.

Journal

Cell 139:352-65 (2009)
DOI:10.1016/j.cell.2009.08.040

Reference

PMID:26563290

Authors

Kinoshita T, Fujita M

Title

Biosynthesis of GPI-anchored proteins: special emphasis on GPI lipid remodeling.

Journal

J Lipid Res 57:6-24 (2016)
DOI:10.1194/jlr.R063313

Related
pathway

ehi00510

N-Glycan biosynthesis

ehi00564

Glycerophospholipid metabolism

KO pathway

ko00563

All links

Genome (1)   
   KEGG GENOME (1)   
Gene (16)   
   KEGG GENES (16)   
All databases (17)   

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