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Database: PDB
Entry: 1A3P
LinkDB: 1A3P
Original site: 1A3P 
HEADER    GROWTH FACTOR                           22-JAN-98   1A3P              
TITLE     ROLE OF THE 6-20 DISULFIDE BRIDGE IN THE STRUCTURE AND ACTIVITY OF    
TITLE    2 EPIDERMAL GROWTH FACTOR, NMR, 20 STRUCTURES                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: EPIDERMAL GROWTH FACTOR;                                   
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: RESIDUES 4 - 48;                                           
COMPND   5 SYNONYM: [ABU6, 20] MEGF4-48;                                        
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;                                        
SOURCE   4 ORGANISM_TAXID: 10090                                                
KEYWDS    GROWTH FACTOR, MURINE EPIDERMAL GROWTH FACTOR, DISULFIDE              
KEYWDS   2 CONNECTIVITIES, EGF-LIKE DOMAIN                                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.BARNHAM,A.TORRES,D.ALEWOOD,P.ALEWOOD,T.DOMAGALA,E.NICE,R.NORTON     
REVDAT   3   14-MAR-18 1A3P    1       REMARK SEQADV                            
REVDAT   2   24-FEB-09 1A3P    1       VERSN                                    
REVDAT   1   29-JUL-98 1A3P    0                                                
JRNL        AUTH   K.J.BARNHAM,A.M.TORRES,D.ALEWOOD,P.F.ALEWOOD,T.DOMAGALA,     
JRNL        AUTH 2 E.C.NICE,R.S.NORTON                                          
JRNL        TITL   ROLE OF THE 6-20 DISULFIDE BRIDGE IN THE STRUCTURE AND       
JRNL        TITL 2 ACTIVITY OF EPIDERMAL GROWTH FACTOR.                         
JRNL        REF    PROTEIN SCI.                  V.   7  1738 1998              
JRNL        REFN                   ISSN 0961-8368                               
JRNL        PMID   10082370                                                     
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.8                                           
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1A3P COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 100 THE DEPOSITION ID IS D_1000170353.                                   
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 300                                
REMARK 210  PH                             : 2.8                                
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : H2O                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NOSEY; DQF-COSY; TOCSY; E-COSY     
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ; 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : AMX                                
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : DYANA, X-PLOR                      
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 1000                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : 20 BEST, BASED ON STEREOCHEMICAL   
REMARK 210                                   AND NOE ENERGIES                   
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING STANDARD 2D METHODS.      
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500  1 HIS A  22   NE2   HIS A  22   CD2    -0.066                       
REMARK 500  2 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500  3 HIS A  22   NE2   HIS A  22   CD2    -0.067                       
REMARK 500  4 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500  5 HIS A  22   NE2   HIS A  22   CD2    -0.067                       
REMARK 500  6 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500  7 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500  8 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500  9 HIS A  22   NE2   HIS A  22   CD2    -0.069                       
REMARK 500 10 HIS A  22   NE2   HIS A  22   CD2    -0.067                       
REMARK 500 11 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500 12 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500 13 HIS A  22   NE2   HIS A  22   CD2    -0.066                       
REMARK 500 14 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500 15 HIS A  22   NE2   HIS A  22   CD2    -0.067                       
REMARK 500 16 HIS A  22   NE2   HIS A  22   CD2    -0.069                       
REMARK 500 17 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500 18 HIS A  22   NE2   HIS A  22   CD2    -0.067                       
REMARK 500 19 HIS A  22   NE2   HIS A  22   CD2    -0.066                       
REMARK 500 20 HIS A  22   NE2   HIS A  22   CD2    -0.068                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 ARG A  45   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.7 DEGREES          
REMARK 500  3 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500  4 ARG A  41   NE  -  CZ  -  NH1 ANGL. DEV. =  -4.5 DEGREES          
REMARK 500  4 ARG A  48   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  5 ARG A  41   NE  -  CZ  -  NH1 ANGL. DEV. =  -4.9 DEGREES          
REMARK 500  5 ARG A  45   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.0 DEGREES          
REMARK 500  5 ARG A  48   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.2 DEGREES          
REMARK 500  6 ARG A  45   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.0 DEGREES          
REMARK 500  7 ARG A  45   NE  -  CZ  -  NH1 ANGL. DEV. =  -4.0 DEGREES          
REMARK 500  8 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.4 DEGREES          
REMARK 500  8 ARG A  45   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.9 DEGREES          
REMARK 500  8 ARG A  48   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.6 DEGREES          
REMARK 500  9 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.0 DEGREES          
REMARK 500 10 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES          
REMARK 500 10 ARG A  48   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.7 DEGREES          
REMARK 500 11 ARG A  48   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500 12 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.6 DEGREES          
REMARK 500 12 ARG A  45   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500 13 TYR A  37   CB  -  CG  -  CD1 ANGL. DEV. =   3.9 DEGREES          
REMARK 500 13 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.0 DEGREES          
REMARK 500 14 ARG A  45   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.6 DEGREES          
REMARK 500 16 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.7 DEGREES          
REMARK 500 16 THR A  44   N   -  CA  -  C   ANGL. DEV. = -17.0 DEGREES          
REMARK 500 16 ARG A  45   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.7 DEGREES          
REMARK 500 17 TYR A  37   CB  -  CG  -  CD1 ANGL. DEV. =   4.2 DEGREES          
REMARK 500 17 ARG A  41   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES          
REMARK 500 18 TYR A  37   CB  -  CG  -  CD1 ANGL. DEV. =   4.7 DEGREES          
REMARK 500 19 TYR A  37   CB  -  CG  -  CD1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500 19 ARG A  48   NE  -  CZ  -  NH1 ANGL. DEV. =  -3.4 DEGREES          
REMARK 500 20 ARG A  45   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   8      -43.56    -19.94                                   
REMARK 500  1 ASN A  16      -81.65   -144.92                                   
REMARK 500  1 ABA A  20       95.80    -58.93                                   
REMARK 500  1 SER A  25      -48.47    -26.11                                   
REMARK 500  1 ASP A  27      -81.50   -146.82                                   
REMARK 500  1 SER A  28     -127.73   -156.16                                   
REMARK 500  1 SER A  38     -130.38   -133.71                                   
REMARK 500  1 ASP A  40       -2.48     92.78                                   
REMARK 500  1 LEU A  47       62.23    -10.76                                   
REMARK 500  2 SER A   8      -53.83    -19.89                                   
REMARK 500  2 ASN A  16      -76.02   -147.86                                   
REMARK 500  2 ABA A  20       95.39    -59.53                                   
REMARK 500  2 GLU A  24      -37.65    -37.84                                   
REMARK 500  2 ASP A  27      -80.04   -149.50                                   
REMARK 500  2 SER A  28     -133.52   -153.46                                   
REMARK 500  2 CYS A  33     -117.73    -96.59                                   
REMARK 500  2 SER A  38     -148.27   -129.74                                   
REMARK 500  2 ASP A  40        8.68    122.81                                   
REMARK 500  2 LEU A  47     -139.03     88.32                                   
REMARK 500  3 ABA A   6       29.06   -145.38                                   
REMARK 500  3 PRO A   7       23.09    -76.47                                   
REMARK 500  3 ASN A  16      -67.63   -146.69                                   
REMARK 500  3 SER A  25      -49.90    -29.93                                   
REMARK 500  3 ASP A  27      -79.42   -149.89                                   
REMARK 500  3 SER A  28     -131.94   -156.05                                   
REMARK 500  3 SER A  38     -144.43   -124.14                                   
REMARK 500  3 ASP A  40        8.91     29.92                                   
REMARK 500  3 CYS A  42       40.53     76.80                                   
REMARK 500  3 LEU A  47      -29.46     70.17                                   
REMARK 500  4 PRO A   7       33.99    -81.87                                   
REMARK 500  4 LEU A  15      -72.75    -94.13                                   
REMARK 500  4 ASN A  16      -56.66   -121.81                                   
REMARK 500  4 ABA A  20       99.25    -59.34                                   
REMARK 500  4 GLU A  24      -37.31    -38.10                                   
REMARK 500  4 ASP A  27      -82.47   -147.28                                   
REMARK 500  4 SER A  28     -125.59   -154.52                                   
REMARK 500  4 SER A  38     -137.04   -139.20                                   
REMARK 500  4 ASP A  40      -15.66    102.06                                   
REMARK 500  4 ARG A  45       90.32    -16.56                                   
REMARK 500  4 LEU A  47     -152.05     67.63                                   
REMARK 500  5 SER A   8      -53.70    -19.86                                   
REMARK 500  5 ASN A  16      -82.05   -144.67                                   
REMARK 500  5 ABA A  20       97.71    -59.10                                   
REMARK 500  5 GLU A  24      -50.51    -26.26                                   
REMARK 500  5 SER A  25      -49.06    -25.90                                   
REMARK 500  5 ASP A  27      -80.24   -146.84                                   
REMARK 500  5 SER A  28     -132.13   -156.80                                   
REMARK 500  5 CYS A  33     -124.71    -94.62                                   
REMARK 500  5 SER A  38     -131.34   -138.17                                   
REMARK 500  5 ASP A  40      -12.70    102.14                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     191 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A  41         0.30    SIDE CHAIN                              
REMARK 500  1 ARG A  45         0.27    SIDE CHAIN                              
REMARK 500  1 ARG A  48         0.30    SIDE CHAIN                              
REMARK 500  2 ARG A  41         0.29    SIDE CHAIN                              
REMARK 500  2 ARG A  45         0.26    SIDE CHAIN                              
REMARK 500  2 ARG A  48         0.30    SIDE CHAIN                              
REMARK 500  3 ARG A  41         0.21    SIDE CHAIN                              
REMARK 500  3 ARG A  45         0.27    SIDE CHAIN                              
REMARK 500  3 ARG A  48         0.32    SIDE CHAIN                              
REMARK 500  4 ARG A  41         0.28    SIDE CHAIN                              
REMARK 500  4 ARG A  45         0.30    SIDE CHAIN                              
REMARK 500  4 ARG A  48         0.30    SIDE CHAIN                              
REMARK 500  5 ARG A  41         0.30    SIDE CHAIN                              
REMARK 500  5 ARG A  45         0.31    SIDE CHAIN                              
REMARK 500  5 ARG A  48         0.28    SIDE CHAIN                              
REMARK 500  6 ARG A  41         0.14    SIDE CHAIN                              
REMARK 500  6 ARG A  45         0.31    SIDE CHAIN                              
REMARK 500  6 ARG A  48         0.31    SIDE CHAIN                              
REMARK 500  7 ARG A  41         0.25    SIDE CHAIN                              
REMARK 500  7 ARG A  45         0.32    SIDE CHAIN                              
REMARK 500  7 ARG A  48         0.28    SIDE CHAIN                              
REMARK 500  8 ARG A  41         0.14    SIDE CHAIN                              
REMARK 500  8 ARG A  45         0.10    SIDE CHAIN                              
REMARK 500  8 ARG A  48         0.29    SIDE CHAIN                              
REMARK 500  9 ARG A  41         0.22    SIDE CHAIN                              
REMARK 500  9 ARG A  45         0.25    SIDE CHAIN                              
REMARK 500  9 ARG A  48         0.30    SIDE CHAIN                              
REMARK 500 10 ARG A  45         0.28    SIDE CHAIN                              
REMARK 500 10 ARG A  48         0.29    SIDE CHAIN                              
REMARK 500 11 ARG A  41         0.26    SIDE CHAIN                              
REMARK 500 11 ARG A  45         0.27    SIDE CHAIN                              
REMARK 500 11 ARG A  48         0.27    SIDE CHAIN                              
REMARK 500 12 ARG A  41         0.18    SIDE CHAIN                              
REMARK 500 12 ARG A  45         0.26    SIDE CHAIN                              
REMARK 500 12 ARG A  48         0.32    SIDE CHAIN                              
REMARK 500 13 ARG A  41         0.23    SIDE CHAIN                              
REMARK 500 13 ARG A  45         0.31    SIDE CHAIN                              
REMARK 500 13 ARG A  48         0.30    SIDE CHAIN                              
REMARK 500 14 ARG A  41         0.24    SIDE CHAIN                              
REMARK 500 14 ARG A  45         0.29    SIDE CHAIN                              
REMARK 500 14 ARG A  48         0.25    SIDE CHAIN                              
REMARK 500 15 ARG A  41         0.28    SIDE CHAIN                              
REMARK 500 15 ARG A  45         0.30    SIDE CHAIN                              
REMARK 500 15 ARG A  48         0.19    SIDE CHAIN                              
REMARK 500 16 ARG A  41         0.24    SIDE CHAIN                              
REMARK 500 16 ARG A  45         0.31    SIDE CHAIN                              
REMARK 500 16 ARG A  48         0.30    SIDE CHAIN                              
REMARK 500 17 ARG A  41         0.15    SIDE CHAIN                              
REMARK 500 17 ARG A  45         0.22    SIDE CHAIN                              
REMARK 500 17 ARG A  48         0.29    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      59 PLANE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1A3P A    4    48  UNP    P01132   EGF_MOUSE      980   1024             
SEQADV 1A3P ABA A    6  UNP  P01132    CYS   982 ENGINEERED MUTATION            
SEQADV 1A3P ABA A   20  UNP  P01132    CYS   996 ENGINEERED MUTATION            
SEQRES   1 A   45  PRO GLY ABA PRO SER SER TYR ASP GLY TYR CYS LEU ASN          
SEQRES   2 A   45  GLY GLY VAL ABA MET HIS ILE GLU SER LEU ASP SER TYR          
SEQRES   3 A   45  THR CYS ASN CYS VAL ILE GLY TYR SER GLY ASP ARG CYS          
SEQRES   4 A   45  GLN THR ARG ASP LEU ARG                                      
MODRES 1A3P ABA A    6  ALA  ALPHA-AMINOBUTYRIC ACID                            
MODRES 1A3P ABA A   20  ALA  ALPHA-AMINOBUTYRIC ACID                            
HET    ABA  A   6      13                                                       
HET    ABA  A  20      13                                                       
HETNAM     ABA ALPHA-AMINOBUTYRIC ACID                                          
FORMUL   1  ABA    2(C4 H9 N O2)                                                
SHEET    1   A 2 VAL A  19  HIS A  22  0                                        
SHEET    2   A 2 TYR A  29  ASN A  32 -1  N  ASN A  32   O  VAL A  19           
SSBOND   1 CYS A   14    CYS A   31                          1555   1555  2.02  
SSBOND   2 CYS A   33    CYS A   42                          1555   1555  2.03  
LINK         N   ABA A   6                 C   GLY A   5     1555   1555  1.31  
LINK         C   ABA A   6                 N   PRO A   7     1555   1555  1.32  
LINK         N   ABA A  20                 C   VAL A  19     1555   1555  1.30  
LINK         C   ABA A  20                 N   MET A  21     1555   1555  1.30  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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