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Database: PDB
Entry: 1AOA
LinkDB: 1AOA
Original site: 1AOA 
HEADER    ACTIN-BINDING PROTEIN                   30-JUN-97   1AOA              
TITLE     N-TERMINAL ACTIN-CROSSLINKING DOMAIN FROM HUMAN FIMBRIN               
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: T-FIMBRIN;                                                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: ABD1                                                       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606                                                 
KEYWDS    ACTIN-BINDING PROTEIN, CALCIUM-BINDING, PHOSPHORYLATION               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    S.C.GOLDSMITH,N.POKALA,W.SHEN,A.A.FEDOROV,P.MATSUDAIRA,               
AUTHOR   2 S.C.ALMO                                                             
REVDAT   3   24-FEB-09 1AOA    1       VERSN                                    
REVDAT   2   01-APR-03 1AOA    1       JRNL                                     
REVDAT   1   31-DEC-97 1AOA    0                                                
JRNL        AUTH   S.C.GOLDSMITH,N.POKALA,W.SHEN,A.A.FEDOROV,                   
JRNL        AUTH 2 P.MATSUDAIRA,S.C.ALMO                                        
JRNL        TITL   THE STRUCTURE OF AN ACTIN-CROSSLINKING DOMAIN FROM           
JRNL        TITL 2 HUMAN FIMBRIN.                                               
JRNL        REF    NAT.STRUCT.BIOL.              V.   4   708 1997              
JRNL        REFN                   ISSN 1072-8368                               
JRNL        PMID   9302997                                                      
JRNL        DOI    10.1038/NSB0997-708                                          
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION.    2.40 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.1                                           
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 2.40                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 8.00                           
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 2.000                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL                           
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : NULL                           
REMARK   3   NUMBER OF REFLECTIONS             : 10458                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : NULL                            
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE            (WORKING SET) : 0.186                           
REMARK   3   FREE R VALUE                     : 0.285                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.000                          
REMARK   3   FREE R VALUE TEST SET COUNT      : NULL                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL                         
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : NULL                         
REMARK   3   BIN FREE R VALUE                    : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL                         
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2313                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 0                                       
REMARK   3   SOLVENT ATOMS            : 20                                      
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 19.30                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL                            
REMARK   3   ESD FROM SIGMAA              (A) : NULL                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.011                           
REMARK   3   BOND ANGLES            (DEGREES) : 1.63                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : 22.69                           
REMARK   3   IMPROPER ANGLES        (DEGREES) : 1.48                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : NULL                                      
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1AOA COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : AUG-95                             
REMARK 200  TEMPERATURE           (KELVIN) : 298                                
REMARK 200  PH                             : 6.5                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : ROTATING ANODE                     
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU                             
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418                             
REMARK 200  MONOCHROMATOR                  : GRAPHITE(002)                      
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : AREA DETECTOR                      
REMARK 200  DETECTOR MANUFACTURER          : SIEMENS                            
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : XDS                                
REMARK 200  DATA SCALING SOFTWARE          : XSCALE                             
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 11764                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 2.400                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 10.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL                               
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 80.0                               
REMARK 200  DATA REDUNDANCY                : NULL                               
REMARK 200  R MERGE                    (I) : 0.05900                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : NULL                               
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.40                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 2.50                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 77.7                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.21700                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: NULL                                           
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MIR                          
REMARK 200 SOFTWARE USED: PHASES, X-PLOR 3.1                                    
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 57.00                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.88                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: PROTEIN WAS CRYSTALLIZED FROM 8-20:      
REMARK 280  PEG 8000, 20 MM CALCIUM ACETATE, 100MM SODIUM CACODYLATE, PH        
REMARK 280  6.5                                                                 
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21                       
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X+1/2,-Y,Z+1/2                                         
REMARK 290       3555   -X,Y+1/2,-Z+1/2                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000       25.01600            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       51.14900            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       30.62050            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       51.14900            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       25.01600            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       30.62050            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLU A   101                                                      
REMARK 465     GLY A   102                                                      
REMARK 465     ILE A   103                                                      
REMARK 465     CYS A   104                                                      
REMARK 465     ALA A   105                                                      
REMARK 465     LEU A   106                                                      
REMARK 465     GLY A   107                                                      
REMARK 465     GLY A   108                                                      
REMARK 465     THR A   109                                                      
REMARK 465     SER A   110                                                      
REMARK 465     GLU A   111                                                      
REMARK 465     LEU A   112                                                      
REMARK 465     SER A   113                                                      
REMARK 465     SER A   114                                                      
REMARK 465     GLU A   115                                                      
REMARK 465     GLY A   116                                                      
REMARK 465     THR A   117                                                      
REMARK 465     GLN A   118                                                      
REMARK 465     HIS A   119                                                      
REMARK 465     SER A   120                                                      
REMARK 465     ALA A   252                                                      
REMARK 465     ALA A   253                                                      
REMARK 465     LEU A   254                                                      
REMARK 465     LEU A   255                                                      
REMARK 465     ARG A   256                                                      
REMARK 465     ASP A   257                                                      
REMARK 465     GLY A   258                                                      
REMARK 465     GLU A   259                                                      
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;            
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     LEU A 245    CG   CD1  CD2                                       
REMARK 470     SER A 246    OG                                                  
REMARK 470     ARG A 247    CG   CD   NE   CZ   NH1  NH2                        
REMARK 470     ASN A 248    CG   OD1  ND2                                       
REMARK 470     GLU A 249    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 251    CG   CD1  CD2                                       
REMARK 470     THR A 260    OG1  CG2                                            
REMARK 470     LEU A 261    CG   CD1  CD2                                       
REMARK 470     GLU A 262    CG   CD   OE1  OE2                                  
REMARK 470     GLU A 263    CG   CD   OE1  OE2                                  
REMARK 470     LEU A 264    CG   CD1  CD2                                       
REMARK 470     MET A 265    CG   SD   CE                                        
REMARK 470     LYS A 266    CG   CD   CE   NZ                                   
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    LEU A 156   CA  -  CB  -  CG  ANGL. DEV. =  14.3 DEGREES          
REMARK 500    ARG A 275   NE  -  CZ  -  NH2 ANGL. DEV. =   3.7 DEGREES          
REMARK 500    GLY A 327   N   -  CA  -  C   ANGL. DEV. = -15.3 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    PRO A 224      -74.25    -33.97                                   
REMARK 500    GLU A 244      -74.04    -81.20                                   
REMARK 500    SER A 246      110.60    176.70                                   
REMARK 500    ARG A 247     -105.43    172.35                                   
REMARK 500    ASN A 248      -92.94     59.68                                   
REMARK 500    PHE A 292       51.79   -113.35                                   
REMARK 500    SER A 293      -75.73   -150.84                                   
REMARK 500    SER A 299      -13.71     74.01                                   
REMARK 500    LYS A 315     -163.15   -178.46                                   
REMARK 500    THR A 331      -53.58     78.46                                   
REMARK 500    PHE A 352      -38.36     72.60                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1AOA A  101   375  UNP    P13797   PLST_HUMAN      98    372             
SEQRES   1 A  275  GLU GLY ILE CYS ALA LEU GLY GLY THR SER GLU LEU SER          
SEQRES   2 A  275  SER GLU GLY THR GLN HIS SER TYR SER GLU GLU GLU LYS          
SEQRES   3 A  275  TYR ALA PHE VAL ASN TRP ILE ASN LYS ALA LEU GLU ASN          
SEQRES   4 A  275  ASP PRO ASP CYS ARG HIS VAL ILE PRO MET ASN PRO ASN          
SEQRES   5 A  275  THR ASP ASP LEU PHE LYS ALA VAL GLY ASP GLY ILE VAL          
SEQRES   6 A  275  LEU CYS LYS MET ILE ASN LEU SER VAL PRO ASP THR ILE          
SEQRES   7 A  275  ASP GLU ARG ALA ILE ASN LYS LYS LYS LEU THR PRO PHE          
SEQRES   8 A  275  ILE ILE GLN GLU ASN LEU ASN LEU ALA LEU ASN SER ALA          
SEQRES   9 A  275  SER ALA ILE GLY CYS HIS VAL VAL ASN ILE GLY ALA GLU          
SEQRES  10 A  275  ASP LEU ARG ALA GLY LYS PRO HIS LEU VAL LEU GLY LEU          
SEQRES  11 A  275  LEU TRP GLN ILE ILE LYS ILE GLY LEU PHE ALA ASP ILE          
SEQRES  12 A  275  GLU LEU SER ARG ASN GLU ALA LEU ALA ALA LEU LEU ARG          
SEQRES  13 A  275  ASP GLY GLU THR LEU GLU GLU LEU MET LYS LEU SER PRO          
SEQRES  14 A  275  GLU GLU LEU LEU LEU ARG TRP ALA ASN PHE HIS LEU GLU          
SEQRES  15 A  275  ASN SER GLY TRP GLN LYS ILE ASN ASN PHE SER ALA ASP          
SEQRES  16 A  275  ILE LYS ASP SER LYS ALA TYR PHE HIS LEU LEU ASN GLN          
SEQRES  17 A  275  ILE ALA PRO LYS GLY GLN LYS GLU GLY GLU PRO ARG ILE          
SEQRES  18 A  275  ASP ILE ASN MET SER GLY PHE ASN GLU THR ASP ASP LEU          
SEQRES  19 A  275  LYS ARG ALA GLU SER MET LEU GLN GLN ALA ASP LYS LEU          
SEQRES  20 A  275  GLY CYS ARG GLN PHE VAL THR PRO ALA ASP VAL VAL SER          
SEQRES  21 A  275  GLY ASN PRO LYS LEU ASN LEU ALA PHE VAL ALA ASN LEU          
SEQRES  22 A  275  PHE ASN                                                      
FORMUL   2  HOH   *20(H2 O)                                                     
HELIX    1   1 GLU A  123  ALA A  136  1                                  14    
HELIX    2   2 ASP A  154  ASP A  162  5                                   9    
HELIX    3   3 ILE A  164  SER A  173  1                                  10    
HELIX    4   4 GLU A  180  ALA A  182  5                                   3    
HELIX    5   5 PRO A  190  ALA A  206  1                                  17    
HELIX    6   6 ALA A  216  ARG A  220  1                                   5    
HELIX    7   7 PRO A  224  GLU A  244  1                                  21    
HELIX    8   8 LEU A  261  LYS A  266  1                                   6    
HELIX    9   9 PRO A  269  ASN A  283  1                                  15    
HELIX   10  10 LYS A  300  ILE A  309  1                                  10    
HELIX   11  11 ASP A  333  ALA A  344  1                                  12    
HELIX   12  12 PRO A  355  VAL A  359  1                                   5    
HELIX   13  13 PRO A  363  LEU A  373  1                                  11    
CISPEP   1 ILE A  147    PRO A  148          0        -0.10                     
CRYST1   50.032   61.241  102.298  90.00  90.00  90.00 P 21 21 21    4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.019987  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.016329  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.009775        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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