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Database: PDB
Entry: 1B9G
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Original site: 1B9G 
HEADER    GROWTH FACTOR                           11-FEB-99   1B9G              
TITLE     INSULIN-LIKE-GROWTH-FACTOR-1                                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PROTEIN (GROWTH FACTOR IGF-1);                             
COMPND   3 CHAIN: A;                                                            
COMPND   4 OTHER_DETAILS: C-REGION DELETED                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606                                                 
KEYWDS    GROWTH FACTOR IGF-1                                                   
EXPDTA    SOLUTION NMR                                                          
AUTHOR    E.DE WOLF,R.GILL,S.GEDDES,J.PITTS,A.WOLLMER,J.GROTZINGER              
REVDAT   2   24-FEB-09 1B9G    1       VERSN                                    
REVDAT   1   23-FEB-99 1B9G    0                                                
JRNL        AUTH   E.DE WOLF,R.GILL,S.GEDDES,J.PITTS,A.WOLLMER,                 
JRNL        AUTH 2 J.GROTZINGER                                                 
JRNL        TITL   SOLUTION STRUCTURE OF A MINI IGF-1.                          
JRNL        REF    PROTEIN SCI.                  V.   5  2193 1996              
JRNL        REFN                   ISSN 0961-8368                               
JRNL        PMID   8931138                                                      
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : DISTANCE GEOMETRY GEOMETRY                           
REMARK   3   AUTHORS     : SCHEEK                                               
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1B9G COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 15-FEB-99.                  
REMARK 100 THE RCSB ID CODE IS RCSB000473.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 293                                
REMARK 210  PH                             : 1.9                                
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ                            
REMARK 210  SPECTROMETER MODEL             : UNITY                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NMRVIEW, SNARF                     
REMARK 210   METHOD USED                   : DISTANCE GEOMETRY                  
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 15                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 1                                  
REMARK 210 CONFORMERS, SELECTION CRITERIA  : LEAST RESTRAINT VIOLATIONS         
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: MEAN STRUCTURE.                                              
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500    TYR A  47   CB  -  CG  -  CD2 ANGL. DEV. =  -3.9 DEGREES          
REMARK 500    CYS A  48   CA  -  CB  -  SG  ANGL. DEV. =   6.8 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    PRO A   2      -76.68   -103.63                                   
REMARK 500    GLU A   3      101.59    104.41                                   
REMARK 500    THR A   4      115.93     57.66                                   
REMARK 500    LEU A   5       26.10   -167.61                                   
REMARK 500    ALA A   8      -43.68     66.04                                   
REMARK 500    CYS A  18       90.15    -55.55                                   
REMARK 500    ASP A  20      -46.55     66.44                                   
REMARK 500    ASN A  26      -21.73    136.26                                   
REMARK 500    PRO A  28     -168.34     15.36                                   
REMARK 500    ILE A  30      -68.37    -90.35                                   
REMARK 500    ARG A  37      163.17     92.39                                   
REMARK 500    CYS A  39      157.85    -37.62                                   
REMARK 500    ARG A  42      -33.54     62.14                                   
REMARK 500    CYS A  48       26.98    -70.76                                   
REMARK 500    ALA A  49       52.30     39.94                                   
REMARK 500    LEU A  51      -96.21    -84.58                                   
REMARK 500    PRO A  53     -124.54     -5.83                                   
REMARK 500    SER A  56       87.13    -67.80                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 CYS A   35     PHE A   36                  146.29                    
REMARK 500 ARG A   42     ARG A   43                  147.86                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: MAIN CHAIN PLANARITY                                       
REMARK 500                                                                      
REMARK 500 THE FOLLOWING RESIDUES HAVE A PSEUDO PLANARITY                       
REMARK 500 TORSION, C(I) - CA(I) - N(I+1) - O(I), GREATER                       
REMARK 500 10.0 DEGREES. (M=MODEL NUMBER; RES=RESIDUE NAME;                     
REMARK 500 C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;                            
REMARK 500 I=INSERTION CODE).                                                   
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        ANGLE                                           
REMARK 500    ARG A  21         12.30                                           
REMARK 500    PHE A  23         11.68                                           
REMARK 500    PHE A  25        -11.01                                           
REMARK 500    PRO A  28         12.02                                           
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1B9G A    1    57  UNP    P01343   IGF1A_HUMAN      1     57             
SEQRES   1 A   57  GLY PRO GLU THR LEU CYS GLY ALA GLU LEU VAL ASP ALA          
SEQRES   2 A   57  LEU GLN PHE VAL CYS GLY ASP ARG GLY PHE TYR PHE ASN          
SEQRES   3 A   57  LYS PRO GLY ILE VAL ASP GLU CYS CYS PHE ARG SER CYS          
SEQRES   4 A   57  ASP LEU ARG ARG LEU GLU MET TYR CYS ALA PRO LEU LYS          
SEQRES   5 A   57  PRO ALA LYS SER ALA                                          
HELIX    1   1 LEU A   10  GLN A   15  1                                   6    
HELIX    2   2 ILE A   30  CYS A   34  1                                   5    
HELIX    3   3 LEU A   44  MET A   46  5                                   3    
SSBOND   1 CYS A    6    CYS A   35                          1555   1555  2.05  
SSBOND   2 CYS A   18    CYS A   48                          1555   1555  2.04  
SSBOND   3 CYS A   34    CYS A   39                          1555   1555  2.05  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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