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Database: PDB
Entry: 1D2L
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Original site: 1D2L 
HEADER    SIGNALING PROTEIN                       24-SEP-99   1D2L              
TITLE     NMR SOLUTION STRUCTURE OF COMPLEMENT-LIKE REPEAT CR3 FROM             
TITLE    2 THE LOW DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN (LRP)           
TITLE    3 . EVIDENCE FOR SPECIFIC BINDING TO THE RECEPTOR BINDING              
TITLE    4 DOMAIN OF HUMAN ALPHA-2 MACROGLOBULIN                                
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: LIPOPROTEIN RECEPTOR RELATED PROTEIN;                      
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: COMPLEMENT-LIKE REPEAT 3 (CR3);                            
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 TISSUE: LIVER;                                                       
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PGEX-2T                                   
KEYWDS    LIGAND BINDING, CALCIUM BINDING, COMPLEMENT-LIKE REPEAT,              
KEYWDS   2 RECEPTOR, SIGNALING PROTEIN                                          
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    K.DOLMER,W.HUANG,P.G.W.GETTINS                                        
REVDAT   4   24-FEB-09 1D2L    1       VERSN                                    
REVDAT   3   01-APR-03 1D2L    1       JRNL                                     
REVDAT   2   21-FEB-00 1D2L    1       JRNL                                     
REVDAT   1   14-JAN-00 1D2L    0                                                
JRNL        AUTH   K.DOLMER,W.HUANG,P.G.GETTINS                                 
JRNL        TITL   NMR SOLUTION STRUCTURE OF COMPLEMENT-LIKE REPEAT             
JRNL        TITL 2 CR3 FROM THE LOW DENSITY LIPOPROTEIN                         
JRNL        TITL 3 RECEPTOR-RELATED PROTEIN. EVIDENCE FOR SPECIFIC              
JRNL        TITL 4 BINDING TO THE RECEPTOR BINDING DOMAIN OF HUMAN              
JRNL        TITL 5 ALPHA(2)-MACROGLOBULIN.                                      
JRNL        REF    J.BIOL.CHEM.                  V. 275  3264 2000              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   10652313                                                     
JRNL        DOI    10.1074/JBC.275.5.3264                                       
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : AMBER 5.0                                            
REMARK   3   AUTHORS     : CASE, D. A., ET AL. (1997) AMBER 5, UNIV. OF         
REMARK   3                 CALIFORNIA, SAN FRANCISCO, UNPUBLISHED               
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON 602 NOE       
REMARK   3  CONSTRAINTS, 9 DISTANCE CONSTRAINTS FOR THE CALCIUM BINDING         
REMARK   3  SITE, 3 DISULFIDE BRIDGES, 10 HYDROGEN BONDS AND FIVE DIHEDRAL      
REMARK   3  ANGLE CONSTRAINTS.                                                  
REMARK   4                                                                      
REMARK   4 1D2L COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 28-SEP-99.                  
REMARK 100 THE RCSB ID CODE IS RCSB009736.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 5.5                                
REMARK 210  IONIC STRENGTH                 : 20 MM NA-D3-ACETATE, 10 MM         
REMARK 210                                   CACL2                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 2 MM CR3 U-15N,13C 20 MM NA-       
REMARK 210                                   D3-ACETATE PH 5.5 10 MM CACL       
REMARK 210                                   90% H2O, 10% D2O                   
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED TOCSY, 3D_        
REMARK 210                                   13C-SEPARATED TOCSY, 3D_15N-       
REMARK 210                                   SEPARATED_NOESY, 3D_13C-           
REMARK 210                                   SEPARATED_NOESY, HBHA(CO)NH,       
REMARK 210                                   CBCA(CO)NH                         
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : DRX                                
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : SYBYL 6.3, DYANA 1.5, PROFIT       
REMARK 210                                   1.8                                
REMARK 210   METHOD USED                   : MOLECULAR DYNAMICS ENERGY          
REMARK 210                                   MINIMIZATION                       
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 40                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS,              
REMARK 210                                   STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR      
REMARK 210  SPECTROSCOPY. 20 STRUCTURES IN THE DEPOSITION WERE FITTED BY        
REMARK 210  FITTING STRUCTURES 2-20 TO THE FIRST, USING PROFIT V1.8             
REMARK 210  (MCLACHLAN, A. D., 1982 ACTA. CRYST.A38, 871-3) AS IMPLEMENTED      
REMARK 210  IN THE PROGRAM PROFIT (MARTIN, A. C. R.,HTTP://                     
REMARK 210  WWW.BIOCHEM.UCL.AC.UK/~MARTIN/PROGRAMS/#PROFIT)                     
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   O    TRP A    23     OD2  ASP A    36              1.85            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   8.8 DEGREES          
REMARK 500  1 CYS A  25   CA  -  CB  -  SG  ANGL. DEV. =   7.3 DEGREES          
REMARK 500  1 ASP A  30   CA  -  CB  -  CG  ANGL. DEV. =  13.7 DEGREES          
REMARK 500  1 CYS A  31   CA  -  CB  -  SG  ANGL. DEV. =   8.2 DEGREES          
REMARK 500  1 ASP A  36   CB  -  CG  -  OD2 ANGL. DEV. =  -5.8 DEGREES          
REMARK 500  2 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   6.7 DEGREES          
REMARK 500  4 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   7.2 DEGREES          
REMARK 500  4 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  5 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   8.0 DEGREES          
REMARK 500  5 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  5 CYS A  25   CA  -  CB  -  SG  ANGL. DEV. =   7.9 DEGREES          
REMARK 500  6 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   6.8 DEGREES          
REMARK 500  6 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.3 DEGREES          
REMARK 500  7 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  8 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   8.2 DEGREES          
REMARK 500  8 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.3 DEGREES          
REMARK 500  8 ASP A  36   CB  -  CG  -  OD2 ANGL. DEV. =  -7.3 DEGREES          
REMARK 500  9 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   7.0 DEGREES          
REMARK 500  9 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500  9 CYS A  25   CA  -  CB  -  SG  ANGL. DEV. =   7.0 DEGREES          
REMARK 500 10 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 11 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.0 DEGREES          
REMARK 500 11 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   7.4 DEGREES          
REMARK 500 11 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 11 CYS A  25   CA  -  CB  -  SG  ANGL. DEV. =   7.7 DEGREES          
REMARK 500 12 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 12 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   8.5 DEGREES          
REMARK 500 13 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 13 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   7.0 DEGREES          
REMARK 500 13 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 13 ASP A  30   C   -  N   -  CA  ANGL. DEV. =  17.0 DEGREES          
REMARK 500 13 ASP A  36   CB  -  CG  -  OD2 ANGL. DEV. =  -7.2 DEGREES          
REMARK 500 14 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 15 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.3 DEGREES          
REMARK 500 15 ASP A  30   C   -  N   -  CA  ANGL. DEV. =  17.4 DEGREES          
REMARK 500 16 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 16 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500 16 CYS A  25   CA  -  CB  -  SG  ANGL. DEV. =   6.8 DEGREES          
REMARK 500 16 ASP A  30   C   -  N   -  CA  ANGL. DEV. =  17.0 DEGREES          
REMARK 500 16 ASP A  36   CB  -  CG  -  OD2 ANGL. DEV. =  -5.8 DEGREES          
REMARK 500 17 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 17 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   6.8 DEGREES          
REMARK 500 17 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 17 ASP A  30   C   -  N   -  CA  ANGL. DEV. =  17.9 DEGREES          
REMARK 500 18 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 18 CYS A  31   CA  -  CB  -  SG  ANGL. DEV. =   6.9 DEGREES          
REMARK 500 19 ARG A  17   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 19 CYS A  18   CA  -  CB  -  SG  ANGL. DEV. =   7.3 DEGREES          
REMARK 500 19 ARG A  22   NE  -  CZ  -  NH1 ANGL. DEV. =   4.6 DEGREES          
REMARK 500 19 ASP A  30   C   -  N   -  CA  ANGL. DEV. =  17.4 DEGREES          
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      56 ANGLE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 GLN A   5      -11.48     81.48                                   
REMARK 500  1 CYS A   6      -50.05   -162.94                                   
REMARK 500  1 GLU A  10     -158.90   -133.29                                   
REMARK 500  1 SER A  16       45.42    126.55                                   
REMARK 500  1 ASP A  30      -60.89     59.61                                   
REMARK 500  1 LEU A  32      -34.03     71.15                                   
REMARK 500  1 PRO A  39       20.99    -69.89                                   
REMARK 500  1 LEU A  41      -74.39   -120.61                                   
REMARK 500  1 CYS A  42      -70.12   -139.92                                   
REMARK 500  1 HIS A  43       86.19     63.87                                   
REMARK 500  2 SER A   2      -36.27    105.66                                   
REMARK 500  2 CYS A   6      -55.50   -120.93                                   
REMARK 500  2 SER A  16       50.75    126.28                                   
REMARK 500  2 LEU A  32      -36.55     62.37                                   
REMARK 500  2 ASN A  34       74.56     59.70                                   
REMARK 500  2 ASP A  36      -67.65    -28.60                                   
REMARK 500  2 PRO A  39       21.66    -68.31                                   
REMARK 500  2 LEU A  41      -80.50   -120.15                                   
REMARK 500  2 CYS A  42      -54.36   -141.74                                   
REMARK 500  2 HIS A  43       99.25     57.15                                   
REMARK 500  3 SER A   2      -58.47    110.55                                   
REMARK 500  3 SER A  16       53.78    128.03                                   
REMARK 500  3 ASN A  29      -13.81    -29.29                                   
REMARK 500  3 LEU A  32      -29.77     69.66                                   
REMARK 500  3 PRO A  39       20.72    -69.12                                   
REMARK 500  3 LEU A  41      -76.07   -123.66                                   
REMARK 500  3 CYS A  42      -67.12   -139.74                                   
REMARK 500  3 HIS A  43      -59.41     56.95                                   
REMARK 500  3 GLN A  44      -65.59     59.45                                   
REMARK 500  4 SER A   2      -56.17   -167.27                                   
REMARK 500  4 SER A  16       48.49    128.36                                   
REMARK 500  4 ASN A  29       52.83    -65.23                                   
REMARK 500  4 LEU A  32      -34.51     63.60                                   
REMARK 500  4 ASN A  34       86.41     56.97                                   
REMARK 500  4 SER A  35      -61.77   -105.05                                   
REMARK 500  4 ASP A  36      -67.46    -29.97                                   
REMARK 500  4 PRO A  39       20.69    -68.09                                   
REMARK 500  4 LEU A  41      -76.62   -125.76                                   
REMARK 500  4 CYS A  42      -71.23   -138.53                                   
REMARK 500  4 HIS A  43       96.07     58.19                                   
REMARK 500  4 GLN A  44       68.57   -153.58                                   
REMARK 500  5 GLN A   5      -27.38     85.41                                   
REMARK 500  5 CYS A   6      -52.52   -152.93                                   
REMARK 500  5 SER A  16       53.45    106.91                                   
REMARK 500  5 ASN A  29       56.93    -67.77                                   
REMARK 500  5 LEU A  32      -35.75     64.16                                   
REMARK 500  5 ASN A  34       80.75     54.58                                   
REMARK 500  5 SER A  35      -76.45    -86.12                                   
REMARK 500  5 ASP A  36      -70.68    -22.49                                   
REMARK 500  5 LEU A  41      -77.09   -125.57                                   
REMARK 500  5 CYS A  42      -61.52   -133.28                                   
REMARK 500  5 HIS A  43       87.70     57.48                                   
REMARK 500  5 GLN A  44      140.77     65.97                                   
REMARK 500  6 GLN A   5      -47.98     76.10                                   
REMARK 500  6 CYS A   6      -55.65   -129.91                                   
REMARK 500  6 GLU A  10     -163.42   -125.58                                   
REMARK 500  6 SER A  16       51.79    128.11                                   
REMARK 500  6 LEU A  32      -33.90     64.38                                   
REMARK 500  6 ASN A  34       83.41     59.23                                   
REMARK 500  6 SER A  35      -61.78   -103.93                                   
REMARK 500  6 PRO A  39       19.87    -67.78                                   
REMARK 500  6 LEU A  41      -79.50   -114.95                                   
REMARK 500  6 CYS A  42      -60.38   -137.28                                   
REMARK 500  6 HIS A  43       86.70     57.59                                   
REMARK 500  6 GLN A  44     -177.96     60.91                                   
REMARK 500  7 SER A   2      -60.82    128.94                                   
REMARK 500  7 SER A  16       51.82    125.59                                   
REMARK 500  7 ASN A  29       -2.68    -45.55                                   
REMARK 500  7 LEU A  32      -34.10     69.28                                   
REMARK 500  7 ASN A  34       81.59     65.40                                   
REMARK 500  7 SER A  35      -60.84    -98.27                                   
REMARK 500  7 ASP A  36      -66.31    -28.57                                   
REMARK 500  7 PRO A  39       22.98    -68.68                                   
REMARK 500  7 LEU A  41      -82.51   -120.46                                   
REMARK 500  7 CYS A  42      -47.91   -144.47                                   
REMARK 500  7 HIS A  43       95.81     56.74                                   
REMARK 500  7 GLN A  44       84.33    -68.03                                   
REMARK 500  8 GLN A   5      -30.51     84.40                                   
REMARK 500  8 CYS A   6      -50.79   -149.89                                   
REMARK 500  8 GLU A  10     -162.34   -128.69                                   
REMARK 500  8 SER A  16       45.17    129.90                                   
REMARK 500  8 CYS A  25       56.89     31.57                                   
REMARK 500  8 ASN A  29       39.55    -61.65                                   
REMARK 500  8 GLU A  37        2.41    -61.18                                   
REMARK 500  8 PRO A  39       21.83    -72.17                                   
REMARK 500  8 LEU A  41      -77.19   -127.21                                   
REMARK 500  8 CYS A  42      -61.99   -138.13                                   
REMARK 500  8 HIS A  43      173.30     58.42                                   
REMARK 500  8 GLN A  44       85.55     39.88                                   
REMARK 500  9 SER A   2      160.35     65.21                                   
REMARK 500  9 GLN A   5      114.21     68.74                                   
REMARK 500  9 CYS A   6      -60.68     67.36                                   
REMARK 500  9 GLU A  10     -159.00   -127.91                                   
REMARK 500  9 SER A  16       44.05    126.77                                   
REMARK 500  9 ASN A  29       53.48    -61.95                                   
REMARK 500  9 LEU A  32      -32.68     63.98                                   
REMARK 500  9 ASN A  34       85.72     57.31                                   
REMARK 500  9 SER A  35      -62.82   -101.83                                   
REMARK 500  9 ASP A  36      -68.95    -29.58                                   
REMARK 500  9 PRO A  39       21.04    -68.82                                   
REMARK 500  9 LEU A  41      -79.37   -118.36                                   
REMARK 500  9 CYS A  42      -66.38   -134.79                                   
REMARK 500  9 HIS A  43       91.57     54.92                                   
REMARK 500 10 GLN A   5      111.15     63.67                                   
REMARK 500 10 CYS A   6      -58.20     60.90                                   
REMARK 500 10 GLU A  10     -154.49   -124.55                                   
REMARK 500 10 SER A  16       53.23    126.23                                   
REMARK 500 10 CYS A  25       58.66     39.14                                   
REMARK 500 10 ASN A  29       52.91    -66.96                                   
REMARK 500 10 LEU A  32      -34.27     62.81                                   
REMARK 500 10 ASN A  34       81.15     51.63                                   
REMARK 500 10 SER A  35      -81.89    -85.62                                   
REMARK 500 10 ASP A  36      -71.33    -16.12                                   
REMARK 500 10 PRO A  39       19.79    -69.76                                   
REMARK 500 10 LEU A  41      -81.48   -117.48                                   
REMARK 500 11 SER A   2      -58.27   -168.37                                   
REMARK 500 11 GLN A   5      -53.36    -17.00                                   
REMARK 500 11 CYS A   6      -45.43   -137.27                                   
REMARK 500 11 SER A  16       44.91    125.57                                   
REMARK 500 11 ASN A  29       63.31    -62.39                                   
REMARK 500 11 LEU A  32      -38.42     65.06                                   
REMARK 500 11 ASN A  34      128.40     62.86                                   
REMARK 500 11 SER A  35      -52.15   -147.47                                   
REMARK 500 11 ASP A  36      -69.46    -28.41                                   
REMARK 500 11 PRO A  39       19.63    -65.52                                   
REMARK 500 11 LEU A  41      -81.20   -108.11                                   
REMARK 500 11 CYS A  42      -59.03   -138.86                                   
REMARK 500 11 HIS A  43       93.47     56.21                                   
REMARK 500 11 GLN A  44       90.26     56.69                                   
REMARK 500 12 GLN A   5      -25.10     87.00                                   
REMARK 500 12 CYS A   6      -50.53   -155.82                                   
REMARK 500 12 GLU A  10     -166.03   -129.37                                   
REMARK 500 12 SER A  16       51.26    122.94                                   
REMARK 500 12 ASN A  29       55.85    -64.09                                   
REMARK 500 12 LEU A  32      -32.62     63.00                                   
REMARK 500 12 ASN A  34       87.06     58.60                                   
REMARK 500 12 SER A  35      -62.89   -106.44                                   
REMARK 500 12 ASP A  36      -67.90    -28.86                                   
REMARK 500 12 PRO A  39       22.10    -68.51                                   
REMARK 500 12 LEU A  41      -82.05   -119.83                                   
REMARK 500 12 CYS A  42      -50.61   -140.02                                   
REMARK 500 12 HIS A  43       98.98     57.31                                   
REMARK 500 13 SER A   2       66.43     32.78                                   
REMARK 500 13 GLN A   5     -105.54     79.34                                   
REMARK 500 13 PRO A   8       84.41    -68.08                                   
REMARK 500 13 SER A  16       52.12    128.31                                   
REMARK 500 13 ASP A  30      -50.10     34.74                                   
REMARK 500 13 LEU A  32      -39.00     69.63                                   
REMARK 500 13 PRO A  39       21.04    -69.76                                   
REMARK 500 13 LEU A  41      -76.56   -121.28                                   
REMARK 500 13 CYS A  42      -68.50   -139.71                                   
REMARK 500 13 HIS A  43      136.52     60.53                                   
REMARK 500 13 GLN A  44      -63.33   -146.93                                   
REMARK 500 14 GLN A   5      117.17     67.17                                   
REMARK 500 14 CYS A   6      -58.94     64.56                                   
REMARK 500 14 GLU A  10     -160.62   -127.39                                   
REMARK 500 14 SER A  16       52.17    125.59                                   
REMARK 500 14 ASN A  29        8.45    -54.79                                   
REMARK 500 14 LEU A  32      -32.28     66.45                                   
REMARK 500 14 PRO A  39       19.69    -67.61                                   
REMARK 500 14 LEU A  41      -75.98   -119.19                                   
REMARK 500 14 CYS A  42      -59.83   -145.92                                   
REMARK 500 14 HIS A  43      -64.22     49.06                                   
REMARK 500 15 PRO A   4       48.33    -77.90                                   
REMARK 500 15 CYS A   6      -71.36     61.62                                   
REMARK 500 15 PRO A   8       78.19    -69.14                                   
REMARK 500 15 SER A  16       55.92    127.95                                   
REMARK 500 15 ASP A  30      -47.52     30.42                                   
REMARK 500 15 LEU A  32      -27.52     70.00                                   
REMARK 500 15 PRO A  39       20.90    -68.89                                   
REMARK 500 15 LEU A  41      -76.86   -120.42                                   
REMARK 500 15 CYS A  42      -61.50   -142.38                                   
REMARK 500 15 HIS A  43      120.52     60.95                                   
REMARK 500 16 SER A   2       59.18     34.73                                   
REMARK 500 16 PRO A   4       47.02    -77.27                                   
REMARK 500 16 CYS A   6      -72.54     61.68                                   
REMARK 500 16 SER A  16       52.05    128.02                                   
REMARK 500 16 ASP A  30      -49.87     35.64                                   
REMARK 500 16 LEU A  32      -25.34     69.62                                   
REMARK 500 16 PRO A  39       21.66    -71.05                                   
REMARK 500 16 LEU A  41      -75.97   -128.72                                   
REMARK 500 16 CYS A  42      -76.42   -132.41                                   
REMARK 500 16 HIS A  43      -48.73     58.32                                   
REMARK 500 17 GLN A   5       63.01    -69.02                                   
REMARK 500 17 CYS A   6      -52.81    121.84                                   
REMARK 500 17 SER A  16       52.39    127.72                                   
REMARK 500 17 ASP A  30      -48.41     28.54                                   
REMARK 500 17 LEU A  32      -30.03     70.36                                   
REMARK 500 17 ASN A  34       88.83     48.62                                   
REMARK 500 17 SER A  35      -68.24    -98.34                                   
REMARK 500 17 ASP A  36      -66.60    -26.65                                   
REMARK 500 17 PRO A  39       21.76    -68.75                                   
REMARK 500 17 LEU A  41      -81.11   -121.58                                   
REMARK 500 17 CYS A  42      -43.00   -146.37                                   
REMARK 500 17 HIS A  43       89.69     54.67                                   
REMARK 500 18 GLN A   5      -43.51     61.84                                   
REMARK 500 18 CYS A   6      -68.17   -103.10                                   
REMARK 500 18 SER A  16       49.11    132.45                                   
REMARK 500 18 ASN A  29       55.59    -65.78                                   
REMARK 500 18 LEU A  32      -39.20     62.20                                   
REMARK 500 18 ASN A  34       78.76     49.70                                   
REMARK 500 18 SER A  35      -76.49    -84.75                                   
REMARK 500 18 ASP A  36      -71.02    -22.55                                   
REMARK 500 18 PRO A  39       20.72    -70.73                                   
REMARK 500 18 LEU A  41      -77.79   -126.64                                   
REMARK 500 18 CYS A  42      -62.94   -136.25                                   
REMARK 500 18 HIS A  43      -55.83     57.86                                   
REMARK 500 18 GLN A  44      -23.79     58.15                                   
REMARK 500 19 GLN A   5      110.89     63.93                                   
REMARK 500 19 CYS A   6      -59.49     66.94                                   
REMARK 500 19 GLU A  10     -156.96   -125.83                                   
REMARK 500 19 SER A  16       51.13    127.56                                   
REMARK 500 19 CYS A  25       65.62     37.88                                   
REMARK 500 19 ASN A  29      -50.02    -24.59                                   
REMARK 500 19 ASP A  30      -49.55     30.75                                   
REMARK 500 19 LEU A  32      -29.55     69.46                                   
REMARK 500 19 ASN A  34       87.55     45.61                                   
REMARK 500 19 SER A  35      -73.86    -86.26                                   
REMARK 500 19 PRO A  39       20.28    -68.79                                   
REMARK 500 19 LEU A  41      -79.98   -125.89                                   
REMARK 500 20 SER A   2      126.25     75.11                                   
REMARK 500 20 GLN A   5      -52.67     57.62                                   
REMARK 500 20 PRO A   8       92.80    -64.14                                   
REMARK 500 20 GLU A  10     -167.94    -78.68                                   
REMARK 500 20 SER A  16       52.90    128.55                                   
REMARK 500 20 ASN A  29       48.42    -64.65                                   
REMARK 500 20 LEU A  32      -30.16     62.19                                   
REMARK 500 20 ASN A  34       82.31     52.83                                   
REMARK 500 20 SER A  35      -72.49    -88.56                                   
REMARK 500 20 PRO A  39       21.45    -71.13                                   
REMARK 500 20 LEU A  41      -76.19   -129.15                                   
REMARK 500 20 CYS A  42      -65.76   -134.57                                   
REMARK 500 20 HIS A  43     -170.07     56.78                                   
REMARK 500 20 GLN A  44       87.49     22.87                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 CYS A   25     ASP A   26          2       145.55                    
REMARK 500 CYS A   25     ASP A   26          3       137.42                    
REMARK 500 ASN A   29     ASP A   30          3       -85.44                    
REMARK 500 CYS A   25     ASP A   26          4       133.09                    
REMARK 500 CYS A   25     ASP A   26          7       145.45                    
REMARK 500 ASN A   29     ASP A   30          7       -91.61                    
REMARK 500 ASN A   29     ASP A   30          8       146.95                    
REMARK 500 CYS A   25     ASP A   26         14       145.12                    
REMARK 500 ASN A   29     ASP A   30         14      -120.75                    
REMARK 500 CYS A   25     ASP A   26         15       140.46                    
REMARK 500 CYS A   25     ASP A   26         17       143.32                    
REMARK 500 GLU A   37     ALA A   38         17       148.31                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  5 ARG A  22         0.15    SIDE_CHAIN                              
REMARK 500  9 ARG A  22         0.09    SIDE_CHAIN                              
REMARK 500 16 ARG A  22         0.12    SIDE_CHAIN                              
REMARK 500 19 ARG A  22         0.10    SIDE_CHAIN                              
REMARK 500 20 ARG A  22         0.11    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620  (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;              
REMARK 620  SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                            
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                           1  CA A  46  CA                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 TRP A  23   O                                                      
REMARK 620 2 ASP A  36   OD2  45.7                                              
REMARK 620 3 ASP A  26   OD1 113.5 158.0                                        
REMARK 620 4 ASP A  30   OD2  93.4 120.8  59.0                                  
REMARK 620 5 ASP A  28   O   154.5 108.9  92.0 101.0                            
REMARK 620 6 GLU A  37   OE2  66.2  58.0 110.9 152.7 104.9                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CA A 46                   
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1CR8   RELATED DB: PDB                                   
REMARK 900 CR3 AND CR8 ARE FROM THE SAME CLUSTER OF COMPLEMENT-LIKE             
REMARK 900 REPEATS FROM LRP                                                     
DBREF  1D2L A    3    45  UNP    Q07954   LRP1_HUMAN     851    893             
SEQADV 1D2L GLY A    1  UNP  Q07954              EXPRESSION ARTIFACT            
SEQADV 1D2L SER A    2  UNP  Q07954              EXPRESSION ARTIFACT            
SEQRES   1 A   45  GLY SER PRO PRO GLN CYS GLN PRO GLY GLU PHE ALA CYS          
SEQRES   2 A   45  ALA ASN SER ARG CYS ILE GLN GLU ARG TRP LYS CYS ASP          
SEQRES   3 A   45  GLY ASP ASN ASP CYS LEU ASP ASN SER ASP GLU ALA PRO          
SEQRES   4 A   45  ALA LEU CYS HIS GLN HIS                                      
HET     CA  A  46       1                                                       
HETNAM      CA CALCIUM ION                                                      
FORMUL   2   CA    CA 2+                                                        
HELIX    1   1 GLN A   20  LYS A   24  5                                   5    
SHEET    1   A 2 PHE A  11  CYS A  13  0                                        
SHEET    2   A 2 ARG A  17  ILE A  19 -1  N  ARG A  17   O  CYS A  13           
SSBOND   1 CYS A    6    CYS A   18                          1555   1555  2.05  
SSBOND   2 CYS A   13    CYS A   31                          1555   1555  2.02  
SSBOND   3 CYS A   25    CYS A   42                          1555   1555  2.02  
LINK        CA    CA A  46                 O   TRP A  23     1555   1555  2.41  
LINK        CA    CA A  46                 OD2 ASP A  36     1555   1555  2.36  
LINK        CA    CA A  46                 OD1 ASP A  26     1555   1555  2.37  
LINK        CA    CA A  46                 OD2 ASP A  30     1555   1555  2.41  
LINK        CA    CA A  46                 O   ASP A  28     1555   1555  2.44  
LINK        CA    CA A  46                 OE2 GLU A  37     1555   1555  2.38  
HYDBND       N   PHE A   11                 O   ILE A   19 1555    1555         
HYDBND       N   CYS A   13                 O   ARG A   17 1555    1555         
HYDBND       N   ARG A   17                 O   CYS A   13 1555    1555         
HYDBND       N   ILE A   19                 O   PHE A   11 1555    1555         
HYDBND       N   GLU A   21                 OE1 GLU A   10 1555    1555         
HYDBND       N   TRP A   23                 O   GLN A   20 1555    1555         
HYDBND       N   LYS A   24                 O   GLU A   21 1555    1555         
HYDBND       N   ALA A   38                 O   SER A   35 1555    1555         
HYDBND       N   LEU A   41                 O   ALA A   38 1555    1555         
HYDBND       N   CYS A   42                 O   ASP A   36 1555    1555         
SITE     1 AC1  7 TRP A  23  ASP A  26  GLY A  27  ASP A  28                    
SITE     2 AC1  7 ASP A  30  ASP A  36  GLU A  37                               
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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