GenomeNet

Database: PDB
Entry: 1EPI
LinkDB: 1EPI
Original site: 1EPI 
HEADER    GROWTH FACTOR                           24-MAR-92   1EPI              
TITLE     THREE-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE STRUCTURES OF MOUSE      
TITLE    2 EPIDERMAL GROWTH FACTOR IN ACIDIC AND PHYSIOLOGICAL PH SOLUTIONS     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: EPIDERMAL GROWTH FACTOR;                                   
COMPND   3 CHAIN: A;                                                            
COMPND   4 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;                                        
SOURCE   4 ORGANISM_TAXID: 10090                                                
KEYWDS    GROWTH FACTOR                                                         
EXPDTA    SOLUTION NMR                                                          
AUTHOR    D.KOHDA,F.INAGAKI                                                     
REVDAT   3   29-NOV-17 1EPI    1       REMARK HELIX                             
REVDAT   2   24-FEB-09 1EPI    1       VERSN                                    
REVDAT   1   31-JAN-94 1EPI    0                                                
JRNL        AUTH   D.KOHDA,F.INAGAKI                                            
JRNL        TITL   THREE-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE STRUCTURES OF   
JRNL        TITL 2 MOUSE EPIDERMAL GROWTH FACTOR IN ACIDIC AND PHYSIOLOGICAL PH 
JRNL        TITL 3 SOLUTIONS.                                                   
JRNL        REF    BIOCHEMISTRY                  V.  31 11928 1992              
JRNL        REFN                   ISSN 0006-2960                               
JRNL        PMID   1445923                                                      
JRNL        DOI    10.1021/BI00162A036                                          
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   D.KOHDA,F.INAGAKI                                            
REMARK   1  TITL   STRUCTURE OF EPIDERMAL GROWTH FACTOR BOUND TO PERDEUTERATED  
REMARK   1  TITL 2 DODECYLPHOSPHOCHOLINE MICELLES DETERMINED BY TWO-DIMENSIONAL 
REMARK   1  TITL 3 NMR AND SIMULATED ANNEALING CALCULATIONS                     
REMARK   1  REF    BIOCHEMISTRY                  V.  31   677 1992              
REMARK   1  REFN                   ISSN 0006-2960                               
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   D.KOHDA,T.SAWADA,F.INAGAKI                                   
REMARK   1  TITL   CHARACTERIZATION OF PH TITRATION SHIFTS FOR ALL THE          
REMARK   1  TITL 2 NONLABILE PROTON RESONANCES IN A PROTEIN BY TWO-DIMENSIONAL  
REMARK   1  TITL 3 NMR: THE CASE OF MOUSE EPIDERMAL GROWTH FACTOR               
REMARK   1  REF    BIOCHEMISTRY                  V.  30  4896 1991              
REMARK   1  REFN                   ISSN 0006-2960                               
REMARK   1 REFERENCE 3                                                          
REMARK   1  AUTH   D.KOHDA,N.GO,K.HAYASHI,F.INAGAKI                             
REMARK   1  TITL   TERTIARY STRUCTURE OF MOUSE EPIDERMAL GROWTH FACTOR          
REMARK   1  TITL 2 DETERMINED BY TWO-DIMENSIONAL 1H NMR                         
REMARK   1  REF    J.BIOCHEM.(TOKYO)             V. 103   741 1988              
REMARK   1  REFN                   ISSN 0021-924X                               
REMARK   1 REFERENCE 4                                                          
REMARK   1  AUTH   D.KOHDA,C.KODAMA,R.KASE,H.NOMOTO,K.HAYASHI,F.INAGAKI         
REMARK   1  TITL   A COMPARATIVE 1H NMR STUDY OF MOUSE ALPHA(1-53) AND          
REMARK   1  TITL 2 BETA(2-53) EPIDERMAL GROWTH FACTORS                          
REMARK   1  REF    BIOCHEM.INT.                  V.  16   647 1988              
REMARK   1  REFN                   ISSN 0158-5231                               
REMARK   1 REFERENCE 5                                                          
REMARK   1  AUTH   D.KOHDA,F.INAGAKI                                            
REMARK   1  TITL   COMPLETE SEQUENCE-SPECIFIC 1H NUCLEAR MAGNETIC RESONANCE     
REMARK   1  TITL 2 ASSIGNMENTS FOR MOUSE EPIDERMAL GROWTH FACTOR                
REMARK   1  REF    J.BIOCHEM.(TOKYO)             V. 103   554 1988              
REMARK   1  REFN                   ISSN 0021-924X                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR                                               
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1EPI COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 100 THE DEPOSITION ID IS D_1000173114.                                   
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : NULL                               
REMARK 210  PH                             : NULL                               
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : NULL                               
REMARK 210  SPECTROMETER MODEL             : NULL                               
REMARK 210  SPECTROMETER MANUFACTURER      : NULL                               
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NULL                               
REMARK 210   METHOD USED                   : NULL                               
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 1                                  
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    HIS A  22   CG    HIS A  22   ND1    -0.101                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    TYR A  37   CB  -  CG  -  CD2 ANGL. DEV. =  -6.0 DEGREES          
REMARK 500    TRP A  49   CG  -  CD1 -  NE1 ANGL. DEV. =  -6.5 DEGREES          
REMARK 500    TRP A  49   CD1 -  NE1 -  CE2 ANGL. DEV. =   6.9 DEGREES          
REMARK 500    TRP A  49   NE1 -  CE2 -  CZ2 ANGL. DEV. =   8.1 DEGREES          
REMARK 500    TRP A  49   NE1 -  CE2 -  CD2 ANGL. DEV. =  -6.1 DEGREES          
REMARK 500    TRP A  50   CD1 -  NE1 -  CE2 ANGL. DEV. =   6.8 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    PRO A   4      108.30    -59.43                                   
REMARK 500    CYS A   6      116.35   -175.17                                   
REMARK 500    PRO A   7     -170.55    -51.24                                   
REMARK 500    SER A   9      -11.02    -45.53                                   
REMARK 500    TYR A  10       52.68   -102.85                                   
REMARK 500    ILE A  23      134.03     57.95                                   
REMARK 500    GLU A  24     -157.91    -57.73                                   
REMARK 500    SER A  25     -128.37     58.25                                   
REMARK 500    ASP A  27     -100.58    -42.90                                   
REMARK 500    CYS A  33     -154.94    -58.57                                   
REMARK 500    ARG A  41       56.94    172.08                                   
REMARK 500    CYS A  42       17.68     59.57                                   
REMARK 500    ASP A  46       46.28    -77.38                                   
REMARK 500    LEU A  47     -157.69     40.02                                   
REMARK 500    TRP A  49      -37.65   -160.58                                   
REMARK 500    TRP A  50      -96.03    -97.65                                   
REMARK 500    GLU A  51     -151.26    -67.26                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500    TYR A  37         0.10    SIDE CHAIN                              
REMARK 500    ARG A  41         0.30    SIDE CHAIN                              
REMARK 500    ARG A  45         0.20    SIDE CHAIN                              
REMARK 500    ARG A  48         0.32    SIDE CHAIN                              
REMARK 500    ARG A  53         0.24    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1EPJ   RELATED DB: PDB                                   
DBREF  1EPI A    1    53  UNP    P01132   EGF_MOUSE      977   1029             
SEQRES   1 A   53  ASN SER TYR PRO GLY CYS PRO SER SER TYR ASP GLY TYR          
SEQRES   2 A   53  CYS LEU ASN GLY GLY VAL CYS MET HIS ILE GLU SER LEU          
SEQRES   3 A   53  ASP SER TYR THR CYS ASN CYS VAL ILE GLY TYR SER GLY          
SEQRES   4 A   53  ASP ARG CYS GLN THR ARG ASP LEU ARG TRP TRP GLU LEU          
SEQRES   5 A   53  ARG                                                          
SHEET    1 SAB 3 SER A   2  PRO A   4  0                                        
SHEET    2 SAB 3 VAL A  19  ILE A  23 -1  O  HIS A  22   N  TYR A   3           
SHEET    3 SAB 3 SER A  28  ASN A  32 -1  O  THR A  30   N  MET A  21           
SHEET    1  SC 2 TYR A  37  SER A  38  0                                        
SHEET    2  SC 2 THR A  44  ARG A  45 -1  O  THR A  44   N  SER A  38           
SSBOND   1 CYS A    6    CYS A   20                          1555   1555  2.01  
SSBOND   2 CYS A   14    CYS A   31                          1555   1555  2.02  
SSBOND   3 CYS A   33    CYS A   42                          1555   1555  2.03  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system