GenomeNet

Database: PDB
Entry: 1FSH
LinkDB: 1FSH
Original site: 1FSH 
HEADER    SIGNALING PROTEIN                       08-SEP-00   1FSH              
TITLE     STRUCTURAL BASIS OF THE RECOGNITION OF THE DISHEVELLED DEP            
TITLE    2 DOMAIN IN THE WNT SIGNALING PATHWAY                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: DISHEVELLED-1;                                             
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: DEP DOMAIN (RESIDUES 391-495);                             
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;                                        
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PQE31                                     
KEYWDS    THREE-HELIX BUNDLE, BETA-ARM, DISHEVELLED-1, SEGMENT                  
KEYWDS   2 POLARITY PROTEIN DISHEVELLED HOMOLOG DVL-1, SIGNALING                
KEYWDS   3 PROTEIN                                                              
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    H.C.WONG,J.ZHENG                                                      
REVDAT   3   24-FEB-09 1FSH    1       VERSN                                    
REVDAT   2   31-DEC-02 1FSH    1       REMARK                                   
REVDAT   1   08-MAR-01 1FSH    0                                                
JRNL        AUTH   H.C.WONG,J.MAO,J.T.NGUYEN,S.SRINIVAS,W.ZHANG,B.LIU,          
JRNL        AUTH 2 L.LI,D.WU,J.ZHENG                                            
JRNL        TITL   STRUCTURAL BASIS OF THE RECOGNITION OF THE                   
JRNL        TITL 2 DISHEVELLED DEP DOMAIN IN THE WNT SIGNALING                  
JRNL        TITL 3 PATHWAY.                                                     
JRNL        REF    NAT.STRUCT.BIOL.              V.   7  1178 2000              
JRNL        REFN                   ISSN 1072-8368                               
JRNL        PMID   11101902                                                     
JRNL        DOI    10.1038/82047                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : DYANA 1.5                                            
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURAL CALCULATIONS ARE           
REMARK   3  BASED ON 2009 NOE DISTANCE RESTRAINTS, 54 HYDROGEN-BOND             
REMARK   3  DISTANCE RESTRAINTS, AND 40 DIHEDRAL ANGLE RESTRAINTS               
REMARK   4                                                                      
REMARK   4 1FSH COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 03-OCT-00.                  
REMARK 100 THE RCSB ID CODE IS RCSB011863.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 300                                
REMARK 210  PH                             : 6.8                                
REMARK 210  IONIC STRENGTH                 : 0.1                                
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.8-1.5MM DEP, 15N AND 13C         
REMARK 210                                   LABELED, 100MM PHOSPHATE           
REMARK 210                                   BUFFER (PH 6.8), 0.1MM NAN3,       
REMARK 210                                   3MM DTT; 0.8-1.5MM DEP, 15N        
REMARK 210                                   LABELED, 100MM PHOSPHATE           
REMARK 210                                   BUFFER (PH 6.8), 0.1MM NAN3,       
REMARK 210                                   3MM DTT; 0.8-1.5MM DEP, VALINE     
REMARK 210                                   SELECTIVE 15N LABELED, 100MM       
REMARK 210                                   PHOSPHATE BUFFER (PH 6.8),         
REMARK 210                                   0.1MM NAN3, 3MM DTT; 0.8-1.5MM     
REMARK 210                                   DEP, 15N LABELED, 100MM            
REMARK 210                                   PHOSPHATE BUFFER (PH 6.8),         
REMARK 210                                   0.1MM NAN3, 3MM DTT                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, HNHA,      
REMARK 210                                   3D_15N-SEPARATED_NOESY, 2D         
REMARK 210                                   NOESY, HMQC                        
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : FELIX 98, XEASY 1.3.13, DYANA      
REMARK 210                                   1.5                                
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 1000                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION                    
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR      
REMARK 210  SPECTROSCOPY.                                                       
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;                   
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                            
REMARK 465   MODELS 1-20                                                        
REMARK 465     RES C SSSEQI                                                     
REMARK 465     THR A     1                                                      
REMARK 465     SER A     2                                                      
REMARK 465     SER A     3                                                      
REMARK 465     VAL A     4                                                      
REMARK 465     PRO A     5                                                      
REMARK 465     GLY A     6                                                      
REMARK 465     ALA A     7                                                      
REMARK 465     PRO A     8                                                      
REMARK 465     GLN A     9                                                      
REMARK 465     LEU A    10                                                      
REMARK 465     GLU A    11                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   CG2  VAL A    17     CE1  PHE A    67              1.45            
REMARK 500   CB   ALA A    13     CG1  VAL A    64              1.57            
REMARK 500   O    SER A    33     NE2  HIS A    63              1.65            
REMARK 500   CG2  VAL A    17     CD1  PHE A    67              1.77            
REMARK 500   OE2  GLU A    12     CG   GLU A    65              1.91            
REMARK 500   CE   MET A    28     CG1  VAL A    56              2.11            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ALA A  13      171.56    -46.10                                   
REMARK 500  1 THR A  16      -48.12   -141.78                                   
REMARK 500  1 VAL A  17      -32.93    167.91                                   
REMARK 500  1 ASP A  20      111.91    -39.39                                   
REMARK 500  1 LEU A  30      135.65    -38.66                                   
REMARK 500  1 GLU A  36      114.17     65.31                                   
REMARK 500  1 ARG A  38      -34.98    178.90                                   
REMARK 500  1 LYS A  44       17.31     83.36                                   
REMARK 500  1 ALA A  48       48.70   -101.76                                   
REMARK 500  1 ASN A  49       93.77    151.58                                   
REMARK 500  1 VAL A  51      -55.36    174.40                                   
REMARK 500  1 ILE A  52      171.94     57.01                                   
REMARK 500  1 ASP A  58      -31.90    -37.71                                   
REMARK 500  1 THR A  62      -72.86    -72.31                                   
REMARK 500  1 VAL A  64     -157.31   -124.48                                   
REMARK 500  1 ARG A  71      -71.16    -71.26                                   
REMARK 500  1 VAL A  90     -168.13   -121.74                                   
REMARK 500  1 LYS A  92      -68.63   -131.18                                   
REMARK 500  1 ILE A  93      -70.35   -149.95                                   
REMARK 500  1 THR A  94       93.66     40.35                                   
REMARK 500  1 PHE A  95      163.40    -41.52                                   
REMARK 500  1 SER A  96      -49.95   -160.72                                   
REMARK 500  1 TYR A 100      121.96    -31.69                                   
REMARK 500  1 PHE A 103      163.82    -39.71                                   
REMARK 500  2 ALA A  13      -51.67    162.37                                   
REMARK 500  2 THR A  16      -43.62   -153.41                                   
REMARK 500  2 VAL A  17      -31.94    167.85                                   
REMARK 500  2 ASP A  20      106.05     56.59                                   
REMARK 500  2 GLU A  36      100.17     71.90                                   
REMARK 500  2 ARG A  38      -36.12    178.98                                   
REMARK 500  2 LYS A  44       17.59     80.99                                   
REMARK 500  2 ALA A  48       47.83    -95.63                                   
REMARK 500  2 ASN A  49       94.85    153.36                                   
REMARK 500  2 VAL A  51      -55.60    174.66                                   
REMARK 500  2 ILE A  52      173.23     54.57                                   
REMARK 500  2 HIS A  63      -75.22   -101.06                                   
REMARK 500  2 VAL A  64     -166.71   -117.26                                   
REMARK 500  2 GLU A  69     -150.63   -159.62                                   
REMARK 500  2 HIS A  88      -98.73   -118.46                                   
REMARK 500  2 THR A  89      -36.05   -156.40                                   
REMARK 500  2 LYS A  92     -111.24   -104.86                                   
REMARK 500  2 ILE A  93      -72.10   -140.54                                   
REMARK 500  2 THR A  94      110.99     55.42                                   
REMARK 500  2 SER A  96      -53.23   -164.28                                   
REMARK 500  2 GLU A  97       41.25     75.71                                   
REMARK 500  2 GLN A  98      -45.19   -132.52                                   
REMARK 500  2 CYS A  99     -144.12    -83.91                                   
REMARK 500  2 PHE A 103      155.60    -35.41                                   
REMARK 500  3 ALA A  13      166.84    -43.28                                   
REMARK 500  3 THR A  16      -49.75   -150.57                                   
REMARK 500  3 VAL A  17      -31.50    167.25                                   
REMARK 500  3 ASP A  20      115.83    -39.48                                   
REMARK 500  3 LEU A  30      138.80    -38.92                                   
REMARK 500  3 ASP A  32       46.93   -141.40                                   
REMARK 500  3 GLU A  36      115.85     68.18                                   
REMARK 500  3 ARG A  38      -34.83    177.84                                   
REMARK 500  3 LYS A  44       16.06     85.96                                   
REMARK 500  3 ASN A  49      100.35    151.30                                   
REMARK 500  3 VAL A  51      -59.50    174.75                                   
REMARK 500  3 ILE A  52      173.06     53.34                                   
REMARK 500  3 LEU A  60      -34.39    -38.00                                   
REMARK 500  3 ARG A  71      -76.04    -64.36                                   
REMARK 500  3 HIS A  88     -111.21   -121.98                                   
REMARK 500  3 LYS A  92     -103.80   -117.00                                   
REMARK 500  3 ILE A  93      -77.53   -147.62                                   
REMARK 500  3 THR A  94      115.53     56.37                                   
REMARK 500  3 SER A  96      -65.69   -165.08                                   
REMARK 500  3 GLU A  97       57.39     72.47                                   
REMARK 500  3 GLN A  98      -44.38   -157.14                                   
REMARK 500  3 CYS A  99     -147.38    -89.56                                   
REMARK 500  3 PHE A 103      155.09    -35.95                                   
REMARK 500  4 ALA A  13      -51.96    162.34                                   
REMARK 500  4 THR A  16      -45.40   -149.79                                   
REMARK 500  4 VAL A  17      -32.92    166.91                                   
REMARK 500  4 ASP A  20      116.77    -38.80                                   
REMARK 500  4 LEU A  30      141.35    -38.88                                   
REMARK 500  4 ASP A  32       50.85   -143.23                                   
REMARK 500  4 GLU A  36      102.30     69.27                                   
REMARK 500  4 ARG A  38      -33.16    176.42                                   
REMARK 500  4 ASN A  49       93.96    151.86                                   
REMARK 500  4 VAL A  51      -53.56    174.33                                   
REMARK 500  4 ILE A  52      174.01     52.69                                   
REMARK 500  4 ASP A  58      -30.56    -39.35                                   
REMARK 500  4 HIS A  63      -73.53   -116.29                                   
REMARK 500  4 VAL A  64     -169.79   -115.50                                   
REMARK 500  4 GLU A  69     -151.59   -162.12                                   
REMARK 500  4 ARG A  71      -70.47    -68.83                                   
REMARK 500  4 HIS A  88     -150.14   -172.29                                   
REMARK 500  4 THR A  89      -29.20    -39.26                                   
REMARK 500  4 ILE A  93      -90.07   -147.34                                   
REMARK 500  4 THR A  94      109.00     54.17                                   
REMARK 500  4 SER A  96      -53.90   -143.43                                   
REMARK 500  4 GLU A  97       36.89     81.13                                   
REMARK 500  4 GLN A  98      -65.71   -136.40                                   
REMARK 500  4 TYR A 100      134.88    -26.45                                   
REMARK 500  4 PHE A 103      163.43    -39.82                                   
REMARK 500  5 ALA A  13      166.64    -43.09                                   
REMARK 500  5 THR A  16      -48.27   -147.67                                   
REMARK 500  5 VAL A  17      -33.00    167.53                                   
REMARK 500  5 ASP A  20      111.67    -39.79                                   
REMARK 500  5 SER A  33      -67.96    -94.57                                   
REMARK 500  5 LEU A  35      -46.67   -156.21                                   
REMARK 500  5 GLU A  36      111.27     57.70                                   
REMARK 500  5 ARG A  38      -33.36    174.92                                   
REMARK 500  5 LYS A  44       17.42     85.74                                   
REMARK 500  5 ASN A  49       89.88    152.07                                   
REMARK 500  5 VAL A  51      -54.59    176.85                                   
REMARK 500  5 ILE A  52      172.03     55.69                                   
REMARK 500  5 LEU A  60      -36.40    -36.71                                   
REMARK 500  5 HIS A  63      -70.01   -124.44                                   
REMARK 500  5 ARG A  70      -35.38    -38.46                                   
REMARK 500  5 ARG A  71      -73.81    -59.66                                   
REMARK 500  5 HIS A  83      -30.32    -39.21                                   
REMARK 500  5 THR A  89       69.37   -115.76                                   
REMARK 500  5 ASN A  91      -31.99    -39.24                                   
REMARK 500  5 LYS A  92     -107.60   -135.60                                   
REMARK 500  5 ILE A  93      -62.46   -131.79                                   
REMARK 500  5 THR A  94       91.88     36.38                                   
REMARK 500  5 SER A  96      -55.16   -162.74                                   
REMARK 500  5 GLU A  97       62.63     86.23                                   
REMARK 500  5 GLN A  98      -43.68   -159.12                                   
REMARK 500  5 TYR A 100      126.84    -31.27                                   
REMARK 500  5 PHE A 103      164.79    -41.02                                   
REMARK 500  6 ALA A  13      167.16    -43.55                                   
REMARK 500  6 LEU A  15     -163.87   -110.74                                   
REMARK 500  6 THR A  16      -51.03   -153.90                                   
REMARK 500  6 VAL A  17      -30.45    166.51                                   
REMARK 500  6 ASP A  20      120.81    -39.46                                   
REMARK 500  6 LEU A  30      138.16    -39.99                                   
REMARK 500  6 GLU A  36      117.43     58.67                                   
REMARK 500  6 ARG A  38      -33.09    173.99                                   
REMARK 500  6 LYS A  44       14.54     87.48                                   
REMARK 500  6 ALA A  48       48.10   -101.49                                   
REMARK 500  6 ASN A  49       90.21    152.42                                   
REMARK 500  6 VAL A  51      -53.57    174.07                                   
REMARK 500  6 ILE A  52      172.13     53.93                                   
REMARK 500  6 ASP A  58      -30.49    -39.57                                   
REMARK 500  6 LEU A  60      -33.37    -37.09                                   
REMARK 500  6 HIS A  63      -67.62   -101.02                                   
REMARK 500  6 ARG A  70      -29.81    -39.05                                   
REMARK 500  6 ARG A  71      -78.32    -63.45                                   
REMARK 500  6 HIS A  83      -31.22    -38.94                                   
REMARK 500  6 THR A  89       60.21   -105.35                                   
REMARK 500  6 LYS A  92      -65.22   -132.24                                   
REMARK 500  6 ILE A  93      -48.13   -161.64                                   
REMARK 500  6 THR A  94       96.05     19.98                                   
REMARK 500  6 PHE A  95      165.71    -42.90                                   
REMARK 500  6 SER A  96      -71.60   -157.59                                   
REMARK 500  6 GLU A  97       64.08     81.90                                   
REMARK 500  6 GLN A  98      -49.81   -158.00                                   
REMARK 500  6 CYS A  99     -142.41    -79.15                                   
REMARK 500  6 PHE A 103      171.77    -43.55                                   
REMARK 500  7 ALA A  13      -50.87    161.55                                   
REMARK 500  7 THR A  16      -41.94   -146.81                                   
REMARK 500  7 VAL A  17      -33.97    172.82                                   
REMARK 500  7 ASP A  20      106.74     55.54                                   
REMARK 500  7 LEU A  30      138.50    -38.12                                   
REMARK 500  7 GLU A  36      110.22     59.55                                   
REMARK 500  7 ARG A  38      -36.73   -178.19                                   
REMARK 500  7 LYS A  44       18.65     86.06                                   
REMARK 500  7 ALA A  48       48.95   -101.86                                   
REMARK 500  7 ASN A  49      105.28    151.57                                   
REMARK 500  7 VAL A  51      -58.85    173.61                                   
REMARK 500  7 ILE A  52      172.81     53.80                                   
REMARK 500  7 TRP A  59      -75.39    -78.95                                   
REMARK 500  7 HIS A  63      -76.37   -115.05                                   
REMARK 500  7 GLU A  69     -168.82   -160.59                                   
REMARK 500  7 ARG A  71      -70.45    -67.64                                   
REMARK 500  7 HIS A  88     -115.93   -130.19                                   
REMARK 500  7 LYS A  92     -125.70    -82.64                                   
REMARK 500  7 ILE A  93      -77.03   -127.22                                   
REMARK 500  7 THR A  94      119.38     56.90                                   
REMARK 500  7 SER A  96      -65.49   -144.40                                   
REMARK 500  7 CYS A  99     -147.42    -99.45                                   
REMARK 500  7 PHE A 103      155.90    -36.08                                   
REMARK 500  8 ALA A  13      -51.38    162.44                                   
REMARK 500  8 THR A  16      -45.90   -155.26                                   
REMARK 500  8 VAL A  17      -31.94    168.51                                   
REMARK 500  8 ASP A  20      103.39     53.99                                   
REMARK 500  8 LEU A  30      137.04    -39.26                                   
REMARK 500  8 GLU A  36       93.16     73.18                                   
REMARK 500  8 ARG A  38      -34.76    177.54                                   
REMARK 500  8 ALA A  48       48.44    -98.83                                   
REMARK 500  8 ASN A  49       96.49    151.95                                   
REMARK 500  8 VAL A  51      -57.86    174.22                                   
REMARK 500  8 ILE A  52      172.79     53.62                                   
REMARK 500  8 TRP A  59      -72.67    -77.18                                   
REMARK 500  8 HIS A  63      -75.82   -117.37                                   
REMARK 500  8 VAL A  64     -169.81   -119.27                                   
REMARK 500  8 GLU A  69     -163.52   -161.79                                   
REMARK 500  8 ARG A  71      -75.56    -68.54                                   
REMARK 500  8 HIS A  88     -112.54   -128.36                                   
REMARK 500  8 LYS A  92      -88.32    -89.61                                   
REMARK 500  8 ILE A  93      -68.78   -153.41                                   
REMARK 500  8 THR A  94      108.44     52.59                                   
REMARK 500  8 SER A  96      -61.02   -146.35                                   
REMARK 500  8 GLU A  97       57.10     80.18                                   
REMARK 500  8 GLN A  98      -44.79   -153.16                                   
REMARK 500  8 CYS A  99     -146.24    -88.71                                   
REMARK 500  8 PHE A 103      163.15    -39.29                                   
REMARK 500  9 ALA A  13      -51.55    162.01                                   
REMARK 500  9 THR A  16      -46.22   -152.85                                   
REMARK 500  9 VAL A  17      -32.54    168.74                                   
REMARK 500  9 ASP A  20      111.93    -39.54                                   
REMARK 500  9 SER A  33      -63.11    -99.02                                   
REMARK 500  9 LEU A  35      -45.69   -152.66                                   
REMARK 500  9 GLU A  36      111.37     57.18                                   
REMARK 500  9 ARG A  38      -33.72    176.07                                   
REMARK 500  9 LYS A  44       18.17     81.29                                   
REMARK 500  9 ALA A  48       54.80   -112.86                                   
REMARK 500  9 ASN A  49       96.34    151.48                                   
REMARK 500  9 VAL A  51      -46.82    173.89                                   
REMARK 500  9 ILE A  52      172.31     53.76                                   
REMARK 500  9 LEU A  60      -38.36    -33.38                                   
REMARK 500  9 HIS A  63      -75.25   -100.58                                   
REMARK 500  9 VAL A  64     -167.27   -115.37                                   
REMARK 500  9 ARG A  71      -74.12    -61.30                                   
REMARK 500  9 HIS A  83      -30.40    -39.18                                   
REMARK 500  9 HIS A  88     -169.92   -108.31                                   
REMARK 500  9 LYS A  92      -67.22   -132.61                                   
REMARK 500  9 ILE A  93      -66.91   -162.90                                   
REMARK 500  9 THR A  94       93.92     41.39                                   
REMARK 500  9 PHE A  95      169.36    -44.87                                   
REMARK 500  9 SER A  96      -47.18   -172.80                                   
REMARK 500  9 GLU A  97       43.75     73.10                                   
REMARK 500  9 GLN A  98      -44.77   -137.21                                   
REMARK 500  9 TYR A 100      122.83    -33.92                                   
REMARK 500  9 PHE A 103      171.39    -43.58                                   
REMARK 500 10 ALA A  13      168.32    -44.10                                   
REMARK 500 10 THR A  16      -50.92   -150.65                                   
REMARK 500 10 VAL A  17      -32.15    169.38                                   
REMARK 500 10 ASP A  20      114.03    -39.13                                   
REMARK 500 10 GLU A  36      103.14     65.86                                   
REMARK 500 10 ARG A  38      -37.42   -175.47                                   
REMARK 500 10 ALA A  48       49.50   -101.68                                   
REMARK 500 10 ASN A  49       88.72    151.50                                   
REMARK 500 10 VAL A  51      -53.64    174.04                                   
REMARK 500 10 ILE A  52      171.24     54.84                                   
REMARK 500 10 ASP A  58      -30.82    -39.18                                   
REMARK 500 10 LEU A  60      -33.77    -36.57                                   
REMARK 500 10 HIS A  63      -67.03   -109.20                                   
REMARK 500 10 ARG A  70      -32.18    -38.92                                   
REMARK 500 10 ARG A  71      -74.77    -61.29                                   
REMARK 500 10 HIS A  83      -29.60    -39.43                                   
REMARK 500 10 VAL A  90     -165.69   -106.38                                   
REMARK 500 10 ASN A  91      -76.93    -45.40                                   
REMARK 500 10 LYS A  92      -71.67   -113.70                                   
REMARK 500 10 ILE A  93      -69.10   -138.11                                   
REMARK 500 10 THR A  94       94.66     35.40                                   
REMARK 500 10 PHE A  95      170.45    -45.10                                   
REMARK 500 10 SER A  96      -72.43   -162.17                                   
REMARK 500 10 GLU A  97       29.70     88.38                                   
REMARK 500 10 GLN A  98      -44.51   -135.08                                   
REMARK 500 10 TYR A 100      127.49    -30.02                                   
REMARK 500 10 PHE A 103      164.05    -39.99                                   
REMARK 500 11 ALA A  13      167.14    -43.44                                   
REMARK 500 11 LEU A  15     -167.28   -112.04                                   
REMARK 500 11 THR A  16      -50.20   -152.64                                   
REMARK 500 11 VAL A  17      -31.51    166.83                                   
REMARK 500 11 SER A  19      140.21    -39.49                                   
REMARK 500 11 ASP A  20      114.44    -39.22                                   
REMARK 500 11 LEU A  30      135.20    -39.67                                   
REMARK 500 11 GLU A  36      107.32     68.64                                   
REMARK 500 11 ARG A  38      -36.08    179.56                                   
REMARK 500 11 ALA A  48       47.66   -103.06                                   
REMARK 500 11 ASN A  49       94.58    152.24                                   
REMARK 500 11 VAL A  51      -52.46    174.45                                   
REMARK 500 11 ILE A  52      173.86     52.83                                   
REMARK 500 11 LEU A  60      -36.88    -38.85                                   
REMARK 500 11 HIS A  63      -60.29   -127.36                                   
REMARK 500 11 ARG A  71      -70.93    -62.95                                   
REMARK 500 11 HIS A  88     -112.14   -113.68                                   
REMARK 500 11 THR A  89       21.85   -154.84                                   
REMARK 500 11 LYS A  92      -65.69   -135.70                                   
REMARK 500 11 ILE A  93      -60.63   -169.73                                   
REMARK 500 11 THR A  94       95.46     40.41                                   
REMARK 500 11 PHE A  95      165.85    -45.58                                   
REMARK 500 11 SER A  96      -63.34   -154.85                                   
REMARK 500 11 GLU A  97       64.74     74.55                                   
REMARK 500 11 GLN A  98      -41.97   -164.48                                   
REMARK 500 11 CYS A  99     -145.86    -90.56                                   
REMARK 500 11 PHE A 103      170.50    -43.39                                   
REMARK 500 12 ALA A  13      173.35    -46.43                                   
REMARK 500 12 THR A  16      -49.68   -150.26                                   
REMARK 500 12 VAL A  17      -31.91    167.63                                   
REMARK 500 12 ASP A  20      122.07    -39.49                                   
REMARK 500 12 LEU A  30      139.61    -37.60                                   
REMARK 500 12 ASP A  32       46.42   -141.33                                   
REMARK 500 12 GLU A  36      105.92     68.21                                   
REMARK 500 12 ARG A  38      -33.92    174.89                                   
REMARK 500 12 LYS A  44        4.11     86.65                                   
REMARK 500 12 ALA A  48       48.13    -96.75                                   
REMARK 500 12 ASN A  49       96.48    153.98                                   
REMARK 500 12 VAL A  51      -54.49    175.37                                   
REMARK 500 12 ILE A  52      173.25     53.26                                   
REMARK 500 12 LEU A  60      -35.84    -37.55                                   
REMARK 500 12 HIS A  63      -65.05   -121.79                                   
REMARK 500 12 ASN A  91      -70.71    -74.64                                   
REMARK 500 12 LYS A  92     -122.35    -65.47                                   
REMARK 500 12 THR A  94       95.15     21.83                                   
REMARK 500 12 SER A  96      -68.67   -143.69                                   
REMARK 500 12 GLN A  98      -46.88   -147.46                                   
REMARK 500 12 CYS A  99     -143.12    -79.15                                   
REMARK 500 12 PHE A 103      158.24    -36.43                                   
REMARK 500 13 ALA A  13      -51.54    162.43                                   
REMARK 500 13 THR A  16      -45.24   -146.08                                   
REMARK 500 13 VAL A  17      -35.22    164.23                                   
REMARK 500 13 ASP A  20      115.01    -38.49                                   
REMARK 500 13 GLU A  36      109.58     59.60                                   
REMARK 500 13 ARG A  38      -32.97    172.24                                   
REMARK 500 13 ASN A  49      102.42    150.35                                   
REMARK 500 13 VAL A  51      -55.21    174.77                                   
REMARK 500 13 ILE A  52      172.15     57.25                                   
REMARK 500 13 TRP A  59      -70.08    -76.52                                   
REMARK 500 13 HIS A  63      -75.31   -109.71                                   
REMARK 500 13 HIS A  88     -165.33   -111.98                                   
REMARK 500 13 LYS A  92      -83.95   -129.35                                   
REMARK 500 13 ILE A  93      -58.12   -147.29                                   
REMARK 500 13 THR A  94       89.51     29.45                                   
REMARK 500 13 PHE A  95      157.66    -39.89                                   
REMARK 500 13 SER A  96      -71.17   -148.78                                   
REMARK 500 13 GLU A  97       42.35     86.70                                   
REMARK 500 13 GLN A  98      -69.92   -129.63                                   
REMARK 500 13 CYS A  99     -132.17    -65.03                                   
REMARK 500 13 PHE A 103      156.22    -35.99                                   
REMARK 500 14 ALA A  13      -52.75    166.17                                   
REMARK 500 14 THR A  16      -43.95   -143.58                                   
REMARK 500 14 VAL A  17      -32.10    168.24                                   
REMARK 500 14 SER A  19      134.21    -39.41                                   
REMARK 500 14 ASP A  20      115.46    -38.04                                   
REMARK 500 14 LEU A  30      136.44    -39.77                                   
REMARK 500 14 GLU A  36      102.44     70.77                                   
REMARK 500 14 ARG A  38      -36.68   -178.13                                   
REMARK 500 14 LYS A  44       17.92     80.07                                   
REMARK 500 14 ASN A  49       87.74    152.19                                   
REMARK 500 14 VAL A  51      -52.77    174.87                                   
REMARK 500 14 ILE A  52      170.89     54.35                                   
REMARK 500 14 LEU A  60      -39.11    -32.81                                   
REMARK 500 14 HIS A  63      -75.59   -100.32                                   
REMARK 500 14 ARG A  71      -70.42    -65.05                                   
REMARK 500 14 LYS A  92     -110.04   -133.97                                   
REMARK 500 14 ILE A  93      -67.12   -132.66                                   
REMARK 500 14 THR A  94       92.66     38.09                                   
REMARK 500 14 PHE A  95      164.23    -42.12                                   
REMARK 500 14 SER A  96      -66.39   -157.48                                   
REMARK 500 14 GLU A  97       31.03     86.99                                   
REMARK 500 14 GLN A  98      -45.13   -138.19                                   
REMARK 500 14 TYR A 100      125.26    -30.37                                   
REMARK 500 14 PHE A 103      159.82    -36.60                                   
REMARK 500 15 ALA A  13      -52.55    164.30                                   
REMARK 500 15 THR A  16      -50.06   -154.17                                   
REMARK 500 15 VAL A  17      -30.91    167.73                                   
REMARK 500 15 ASP A  20      110.54    -38.97                                   
REMARK 500 15 LEU A  30      133.65    -38.92                                   
REMARK 500 15 GLU A  36      118.00     58.93                                   
REMARK 500 15 ARG A  38      -34.08    176.10                                   
REMARK 500 15 ASN A  49      100.19    150.99                                   
REMARK 500 15 VAL A  51      -49.48    175.82                                   
REMARK 500 15 ILE A  52      172.95     52.87                                   
REMARK 500 15 ASP A  58      -29.29    -39.94                                   
REMARK 500 15 HIS A  63      -72.64   -101.23                                   
REMARK 500 15 ARG A  71      -72.03    -67.57                                   
REMARK 500 15 HIS A  83      -37.53    -38.36                                   
REMARK 500 15 HIS A  88     -168.56   -115.15                                   
REMARK 500 15 LYS A  92      -73.10   -136.80                                   
REMARK 500 15 ILE A  93      -68.39   -148.60                                   
REMARK 500 15 THR A  94      100.03     46.35                                   
REMARK 500 15 PHE A  95      166.63    -48.94                                   
REMARK 500 15 SER A  96      -54.35   -165.52                                   
REMARK 500 15 CYS A  99     -147.55    -99.74                                   
REMARK 500 15 PHE A 103     -176.18    -59.63                                   
REMARK 500 16 ALA A  13      169.64    -44.09                                   
REMARK 500 16 VAL A  17      -33.89    168.58                                   
REMARK 500 16 ASP A  20      118.75    -39.61                                   
REMARK 500 16 GLU A  36       87.22     65.99                                   
REMARK 500 16 ARG A  38      -36.15    174.07                                   
REMARK 500 16 ALA A  48       47.76   -107.41                                   
REMARK 500 16 ASN A  49       95.74    151.50                                   
REMARK 500 16 VAL A  51      -55.86    174.89                                   
REMARK 500 16 ILE A  52      173.11     53.18                                   
REMARK 500 16 HIS A  63      -68.71    -96.48                                   
REMARK 500 16 GLU A  69     -169.01   -162.73                                   
REMARK 500 16 ARG A  71      -73.89    -62.96                                   
REMARK 500 16 HIS A  88     -112.85   -112.81                                   
REMARK 500 16 LYS A  92      -90.25    -71.03                                   
REMARK 500 16 ILE A  93      -66.66   -172.65                                   
REMARK 500 16 THR A  94      116.93     54.21                                   
REMARK 500 16 SER A  96      -63.41   -142.30                                   
REMARK 500 16 GLU A  97       58.16     85.07                                   
REMARK 500 16 GLN A  98      -44.30   -159.74                                   
REMARK 500 16 CYS A  99     -145.77    -83.07                                   
REMARK 500 16 PHE A 103      161.81    -38.61                                   
REMARK 500 17 ALA A  13      -53.30    165.17                                   
REMARK 500 17 THR A  16      -45.95   -143.61                                   
REMARK 500 17 VAL A  17      -34.28    162.91                                   
REMARK 500 17 ASP A  20      117.89    -39.37                                   
REMARK 500 17 GLU A  36      119.00     66.95                                   
REMARK 500 17 ARG A  38      -38.33   -177.74                                   
REMARK 500 17 ALA A  48       49.26   -108.90                                   
REMARK 500 17 ASN A  49      101.91    151.76                                   
REMARK 500 17 VAL A  51      -56.30   -179.31                                   
REMARK 500 17 ILE A  52      175.12     57.30                                   
REMARK 500 17 ASP A  58      -30.12    -39.45                                   
REMARK 500 17 HIS A  63      -73.85   -115.34                                   
REMARK 500 17 GLU A  69     -160.01   -163.62                                   
REMARK 500 17 ARG A  71      -70.50    -67.85                                   
REMARK 500 17 HIS A  88     -147.66   -179.54                                   
REMARK 500 17 THR A  89      -31.41    -38.01                                   
REMARK 500 17 LYS A  92      -57.66   -126.50                                   
REMARK 500 17 ILE A  93      -76.29   -146.23                                   
REMARK 500 17 THR A  94       92.14     35.83                                   
REMARK 500 17 SER A  96      -67.53   -170.79                                   
REMARK 500 17 GLU A  97       23.07     86.12                                   
REMARK 500 17 GLN A  98      -68.63   -108.31                                   
REMARK 500 17 CYS A  99     -131.65    -69.75                                   
REMARK 500 17 PHE A 103      156.48    -36.78                                   
REMARK 500 18 ALA A  13      -51.51    161.96                                   
REMARK 500 18 THR A  16      -47.42   -151.02                                   
REMARK 500 18 VAL A  17      -32.69    168.52                                   
REMARK 500 18 ASP A  20      114.75    -39.60                                   
REMARK 500 18 LEU A  30      135.89    -39.86                                   
REMARK 500 18 GLU A  36      116.63     63.46                                   
REMARK 500 18 ARG A  38      -35.09    178.38                                   
REMARK 500 18 LYS A  44       16.93     85.12                                   
REMARK 500 18 ASN A  49       94.99    151.38                                   
REMARK 500 18 VAL A  51      -55.03    174.14                                   
REMARK 500 18 ILE A  52      171.05     55.93                                   
REMARK 500 18 LEU A  60      -36.25    -34.48                                   
REMARK 500 18 HIS A  63      -73.79   -104.50                                   
REMARK 500 18 VAL A  64     -169.96   -118.85                                   
REMARK 500 18 ARG A  71      -73.96    -64.10                                   
REMARK 500 18 HIS A  88     -139.40   -105.31                                   
REMARK 500 18 LYS A  92     -113.25    -86.01                                   
REMARK 500 18 ILE A  93      -78.52   -139.77                                   
REMARK 500 18 THR A  94      120.81     58.84                                   
REMARK 500 18 SER A  96      -60.04   -172.55                                   
REMARK 500 18 GLU A  97       34.69     78.72                                   
REMARK 500 18 GLN A  98      -46.04   -138.39                                   
REMARK 500 18 TYR A 100      128.75    -32.41                                   
REMARK 500 18 PHE A 103      163.76    -40.06                                   
REMARK 500 19 ALA A  13      -53.35    166.88                                   
REMARK 500 19 THR A  16      -46.43   -151.97                                   
REMARK 500 19 VAL A  17      -34.03    166.55                                   
REMARK 500 19 ASP A  20      119.12    -38.69                                   
REMARK 500 19 LEU A  30      135.21    -38.23                                   
REMARK 500 19 SER A  33      -60.07    -97.76                                   
REMARK 500 19 GLU A  36      118.33     59.16                                   
REMARK 500 19 ARG A  38      -34.68    178.45                                   
REMARK 500 19 LYS A  44       16.63     85.11                                   
REMARK 500 19 ALA A  48       49.18    -96.36                                   
REMARK 500 19 ASN A  49       89.76    152.86                                   
REMARK 500 19 VAL A  51      -53.80    174.06                                   
REMARK 500 19 ILE A  52      173.08     52.89                                   
REMARK 500 19 LEU A  60      -38.83    -34.82                                   
REMARK 500 19 HIS A  63      -74.34   -106.96                                   
REMARK 500 19 VAL A  64     -169.58   -120.03                                   
REMARK 500 19 ARG A  71      -73.11    -69.25                                   
REMARK 500 19 HIS A  88     -169.30   -111.42                                   
REMARK 500 19 LYS A  92      -70.26   -131.70                                   
REMARK 500 19 ILE A  93      -66.50   -154.84                                   
REMARK 500 19 THR A  94       91.48     37.62                                   
REMARK 500 19 PHE A  95      158.41    -40.69                                   
REMARK 500 19 SER A  96      -71.39   -146.10                                   
REMARK 500 19 GLU A  97       53.75     82.63                                   
REMARK 500 19 GLN A  98      -57.03   -152.26                                   
REMARK 500 19 CYS A  99     -141.52    -70.70                                   
REMARK 500 19 PHE A 103      165.58    -41.31                                   
REMARK 500 20 ALA A  13      167.95    -44.05                                   
REMARK 500 20 LEU A  15     -169.32   -117.92                                   
REMARK 500 20 THR A  16      -50.15   -150.77                                   
REMARK 500 20 VAL A  17      -31.77    168.29                                   
REMARK 500 20 ASP A  20      114.94    -39.20                                   
REMARK 500 20 LEU A  30      136.73    -38.67                                   
REMARK 500 20 SER A  33      -61.90    -92.65                                   
REMARK 500 20 GLU A  36      118.24     61.55                                   
REMARK 500 20 ARG A  38      -35.65    178.89                                   
REMARK 500 20 ASN A  49      108.65    151.86                                   
REMARK 500 20 VAL A  51      -53.93    174.91                                   
REMARK 500 20 ILE A  52      172.64     54.53                                   
REMARK 500 20 LEU A  60      -37.55    -39.64                                   
REMARK 500 20 ARG A  71      -73.89    -63.63                                   
REMARK 500 20 HIS A  88     -109.15   -112.17                                   
REMARK 500 20 THR A  89      -31.00   -136.37                                   
REMARK 500 20 VAL A  90     -179.11    -58.37                                   
REMARK 500 20 LYS A  92      -78.23    -76.45                                   
REMARK 500 20 ILE A  93      -54.14   -179.45                                   
REMARK 500 20 THR A  94      100.84     51.15                                   
REMARK 500 20 SER A  96      -48.67   -170.92                                   
REMARK 500 20 GLU A  97       17.82     87.15                                   
REMARK 500 20 TYR A 100      134.17    -34.92                                   
REMARK 500 20 PHE A 103      161.76    -39.23                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1FSH A    1   105  UNP    P51141   DVL1_MOUSE     391    495             
SEQRES   1 A  105  THR SER SER VAL PRO GLY ALA PRO GLN LEU GLU GLU ALA          
SEQRES   2 A  105  PRO LEU THR VAL LYS SER ASP MET SER ALA ILE VAL ARG          
SEQRES   3 A  105  VAL MET GLN LEU PRO ASP SER GLY LEU GLU ILE ARG ASP          
SEQRES   4 A  105  ARG MET TRP LEU LYS ILE THR ILE ALA ASN ALA VAL ILE          
SEQRES   5 A  105  GLY ALA ASP VAL VAL ASP TRP LEU TYR THR HIS VAL GLU          
SEQRES   6 A  105  GLY PHE LYS GLU ARG ARG GLU ALA ARG LYS TYR ALA SER          
SEQRES   7 A  105  SER MET LEU LYS HIS GLY PHE LEU ARG HIS THR VAL ASN          
SEQRES   8 A  105  LYS ILE THR PHE SER GLU GLN CYS TYR TYR VAL PHE GLY          
SEQRES   9 A  105  ASP                                                          
HELIX    1   1 ASP A   20  LEU A   30  1                                  11    
HELIX    2   2 VAL A   51  VAL A   64  1                                  14    
HELIX    3   3 GLU A   69  HIS A   83  1                                  15    
SHEET    1   A 2 ARG A  40  TRP A  42  0                                        
SHEET    2   A 2 ILE A  45  ILE A  47 -1  N  ILE A  45   O  TRP A  42           
SHEET    1   B 2 LEU A  86  ARG A  87  0                                        
SHEET    2   B 2 VAL A 102  PHE A 103 -1  N  VAL A 102   O  ARG A  87           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system