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Database: PDB
Entry: 1KJJ
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HEADER    TRANSFERASE                             04-DEC-01   1KJJ              
TITLE     CRYSTAL STRUCTURE OF GLYCNIAMIDE RIBONUCLEOTIDE TRANSFORMYLASE IN     
TITLE    2 COMPLEX WITH MG-ATP-GAMMA-S                                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE 2;             
COMPND   3 CHAIN: A, B;                                                         
COMPND   4 SYNONYM: GART 2; GAR TRANSFORMYLASE 2; 5'-PHOSPHORIBOSYLGLYCINAMIDE; 
COMPND   5 EC: 2.1.2.-;                                                         
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   3 ORGANISM_TAXID: 562;                                                 
SOURCE   4 GENE: PURT;                                                          
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21(DE3)PLYSS;                            
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PET-22B                                   
KEYWDS    ATP-GRASP, PURINE BIOSYNTHESIS, NUCLEOTIDE, TRANSFERASE               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    J.B.THODEN,S.M.FIRESTINE,S.J.BENKOVIC,H.M.HOLDEN                      
REVDAT   4   11-OCT-17 1KJJ    1       REMARK                                   
REVDAT   3   24-FEB-09 1KJJ    1       VERSN                                    
REVDAT   2   01-APR-03 1KJJ    1       JRNL                                     
REVDAT   1   28-JUN-02 1KJJ    0                                                
JRNL        AUTH   J.B.THODEN,S.M.FIRESTINE,S.J.BENKOVIC,H.M.HOLDEN             
JRNL        TITL   PURT-ENCODED GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE.      
JRNL        TITL 2 ACCOMMODATION OF ADENOSINE NUCLEOTIDE ANALOGS WITHIN THE     
JRNL        TITL 3 ACTIVE SITE.                                                 
JRNL        REF    J.BIOL.CHEM.                  V. 277 23898 2002              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   11953435                                                     
JRNL        DOI    10.1074/JBC.M202251200                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION.    1.75 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : TNT                                                  
REMARK   3   AUTHORS     : TRONRUD,TEN EYCK,MATTHEWS                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.75                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 30.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 96.0                           
REMARK   3   NUMBER OF REFLECTIONS             : 82695                          
REMARK   3                                                                      
REMARK   3  USING DATA ABOVE SIGMA CUTOFF.                                      
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.187                           
REMARK   3   R VALUE            (WORKING SET) : 0.184                           
REMARK   3   FREE R VALUE                     : 0.233                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.000                          
REMARK   3   FREE R VALUE TEST SET COUNT      : 8270                            
REMARK   3                                                                      
REMARK   3  USING ALL DATA, NO SIGMA CUTOFF.                                    
REMARK   3   R VALUE   (WORKING + TEST SET, NO CUTOFF) : 0.1870                 
REMARK   3   R VALUE          (WORKING SET, NO CUTOFF) : NULL                   
REMARK   3   FREE R VALUE                  (NO CUTOFF) : NULL                   
REMARK   3   FREE R VALUE TEST SET SIZE (%, NO CUTOFF) : NULL                   
REMARK   3   FREE R VALUE TEST SET COUNT   (NO CUTOFF) : NULL                   
REMARK   3   TOTAL NUMBER OF REFLECTIONS   (NO CUTOFF) : 82695                  
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 5886                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 81                                      
REMARK   3   SOLVENT ATOMS            : 674                                     
REMARK   3                                                                      
REMARK   3  WILSON B VALUE (FROM FCALC, A**2) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.    RMS    WEIGHT  COUNT           
REMARK   3   BOND LENGTHS                 (A) : 0.014 ; NULL  ; NULL            
REMARK   3   BOND ANGLES            (DEGREES) : 2.600 ; NULL  ; NULL            
REMARK   3   TORSION ANGLES         (DEGREES) : NULL  ; NULL  ; NULL            
REMARK   3   PSEUDOROTATION ANGLES  (DEGREES) : NULL  ; NULL  ; NULL            
REMARK   3   TRIGONAL CARBON PLANES       (A) : NULL  ; NULL  ; NULL            
REMARK   3   GENERAL PLANES               (A) : NULL  ; NULL  ; NULL            
REMARK   3   ISOTROPIC THERMAL FACTORS (A**2) : NULL  ; NULL  ; NULL            
REMARK   3   NON-BONDED CONTACTS          (A) : NULL  ; NULL  ; NULL            
REMARK   3                                                                      
REMARK   3  INCORRECT CHIRAL-CENTERS (COUNT) : NULL                             
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELING.                                              
REMARK   3   METHOD USED : NULL                                                 
REMARK   3   KSOL        : NULL                                                 
REMARK   3   BSOL        : NULL                                                 
REMARK   3                                                                      
REMARK   3  RESTRAINT LIBRARIES.                                                
REMARK   3   STEREOCHEMISTRY : ENGH & HUBER                                     
REMARK   3   ISOTROPIC THERMAL FACTOR RESTRAINTS : NULL                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1KJJ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-DEC-01.                  
REMARK 100 THE DEPOSITION ID IS D_1000015016.                                   
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 06-NOV-00                          
REMARK 200  TEMPERATURE           (KELVIN) : 110                                
REMARK 200  PH                             : 6.7                                
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : ROTATING ANODE                     
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU RU200                       
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.54178                            
REMARK 200  MONOCHROMATOR                  : GOEBEL OPTICS                      
REMARK 200  OPTICS                         : GOEBEL OPTICS                      
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : AREA DETECTOR                      
REMARK 200  DETECTOR MANUFACTURER          : SIEMENS HI-STAR                    
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : FRAMBO                             
REMARK 200  DATA SCALING SOFTWARE          : SAINT                              
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 82695                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.750                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 30.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 96.0                               
REMARK 200  DATA REDUNDANCY                : 2.500                              
REMARK 200  R MERGE                    (I) : 0.06000                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR THE DATA SET  : 18.2000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.75                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.87                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 88.0                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 1.40                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.21500                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200  <I/SIGMA(I)> FOR SHELL         : 4.400                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: FOURIER SYNTHESIS            
REMARK 200 SOFTWARE USED: TNT                                                   
REMARK 200 STARTING MODEL: PDB ENTRY 1EYZ                                       
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 50.64                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.49                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: PEG 5000, NACL, MGCL2, MOPS, ATP-GAMMA   
REMARK 280  -S, PH 6.7, BATCH AT 277K                                           
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 2                        
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,Z                                                 
REMARK 290       3555   -X+1/2,Y+1/2,-Z                                         
REMARK 290       4555   X+1/2,-Y+1/2,-Z                                         
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000       30.95000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       89.65000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       30.95000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000       89.65000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: DIMERIC                           
REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: DIMERIC                    
REMARK 350 SOFTWARE USED: PISA                                                  
REMARK 350 TOTAL BURIED SURFACE AREA: 7390 ANGSTROM**2                          
REMARK 350 SURFACE AREA OF THE COMPLEX: 27480 ANGSTROM**2                       
REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -77.0 KCAL/MOL                        
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     GLY A   185                                                      
REMARK 465     ARG A   186                                                      
REMARK 465     ALA A   187                                                      
REMARK 465     GLY A   188                                                      
REMARK 465     ALA A   189                                                      
REMARK 465     GLY B   184                                                      
REMARK 465     GLY B   185                                                      
REMARK 465     ARG B   186                                                      
REMARK 465     ALA B   187                                                      
REMARK 465     GLY B   188                                                      
REMARK 465     ALA B   189                                                      
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   NH2  ARG B   191     NH2  ARG B   191     2555     1.72            
REMARK 500   O    HOH B   639     O    HOH B   639     2655     1.86            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    GLU A  30   CD    GLU A  30   OE2     0.092                       
REMARK 500    GLU A  37   CD    GLU A  37   OE2     0.073                       
REMARK 500    GLU A  72   CD    GLU A  72   OE2     0.083                       
REMARK 500    GLU A  95   CD    GLU A  95   OE2     0.067                       
REMARK 500    GLU A  96   CD    GLU A  96   OE2     0.080                       
REMARK 500    GLU B  82   CD    GLU B  82   OE2     0.069                       
REMARK 500    GLU B  96   CD    GLU B  96   OE2     0.093                       
REMARK 500    GLU B  97   CD    GLU B  97   OE2     0.071                       
REMARK 500    GLU B 138   CD    GLU B 138   OE2     0.070                       
REMARK 500    GLU B 143   CD    GLU B 143   OE2     0.075                       
REMARK 500    GLU B 172   CD    GLU B 172   OE2     0.087                       
REMARK 500    GLU B 226   CD    GLU B 226   OE2     0.083                       
REMARK 500    GLU B 299   CD    GLU B 299   OE1    -0.073                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    ARG A  14   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500    ASP A  42   CB  -  CG  -  OD1 ANGL. DEV. =   7.9 DEGREES          
REMARK 500    ASP A  42   CB  -  CG  -  OD2 ANGL. DEV. =  -6.8 DEGREES          
REMARK 500    ASP A  65   CB  -  CG  -  OD2 ANGL. DEV. =  -6.1 DEGREES          
REMARK 500    ARG A 114   NE  -  CZ  -  NH1 ANGL. DEV. =   4.7 DEGREES          
REMARK 500    ARG A 114   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.9 DEGREES          
REMARK 500    ARG A 119   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.1 DEGREES          
REMARK 500    ARG A 133   NE  -  CZ  -  NH1 ANGL. DEV. =   3.0 DEGREES          
REMARK 500    ASP A 136   CB  -  CG  -  OD2 ANGL. DEV. =  -9.1 DEGREES          
REMARK 500    ARG A 169   NE  -  CZ  -  NH1 ANGL. DEV. =   4.8 DEGREES          
REMARK 500    ARG A 169   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.6 DEGREES          
REMARK 500    ARG A 191   NE  -  CZ  -  NH1 ANGL. DEV. =   4.2 DEGREES          
REMARK 500    ASP A 201   CB  -  CG  -  OD1 ANGL. DEV. =   6.6 DEGREES          
REMARK 500    ASP A 201   CB  -  CG  -  OD2 ANGL. DEV. =  -6.2 DEGREES          
REMARK 500    ASP A 227   CB  -  CG  -  OD1 ANGL. DEV. =   7.9 DEGREES          
REMARK 500    ASP A 227   CB  -  CG  -  OD2 ANGL. DEV. =  -5.7 DEGREES          
REMARK 500    ASP A 229   CB  -  CG  -  OD1 ANGL. DEV. =   5.6 DEGREES          
REMARK 500    ASP A 229   CB  -  CG  -  OD2 ANGL. DEV. =  -8.1 DEGREES          
REMARK 500    ARG A 246   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500    ARG A 252   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500    ARG A 283   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500    ARG A 283   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.0 DEGREES          
REMARK 500    ASP A 286   CB  -  CG  -  OD1 ANGL. DEV. =   9.5 DEGREES          
REMARK 500    ASP A 286   CB  -  CG  -  OD2 ANGL. DEV. =  -6.7 DEGREES          
REMARK 500    ASP A 296   CB  -  CG  -  OD1 ANGL. DEV. =   6.5 DEGREES          
REMARK 500    ASP A 296   CB  -  CG  -  OD2 ANGL. DEV. =  -5.6 DEGREES          
REMARK 500    ARG A 305   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500    ARG A 316   NE  -  CZ  -  NH1 ANGL. DEV. =   3.8 DEGREES          
REMARK 500    VAL A 340   CA  -  CB  -  CG1 ANGL. DEV. = -11.9 DEGREES          
REMARK 500    ASP A 347   CB  -  CG  -  OD1 ANGL. DEV. =   7.3 DEGREES          
REMARK 500    ASP A 359   CB  -  CG  -  OD2 ANGL. DEV. =  -6.1 DEGREES          
REMARK 500    ARG A 363   NE  -  CZ  -  NH1 ANGL. DEV. =   5.8 DEGREES          
REMARK 500    ARG A 363   NE  -  CZ  -  NH2 ANGL. DEV. =  -6.3 DEGREES          
REMARK 500    ARG A 380   NE  -  CZ  -  NH2 ANGL. DEV. =   3.4 DEGREES          
REMARK 500    ARG B  14   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES          
REMARK 500    ARG B  33   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500    ARG B  33   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.0 DEGREES          
REMARK 500    ASP B  42   CB  -  CG  -  OD1 ANGL. DEV. =   6.4 DEGREES          
REMARK 500    ASP B  42   CB  -  CG  -  OD2 ANGL. DEV. =  -5.7 DEGREES          
REMARK 500    ARG B  43   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.6 DEGREES          
REMARK 500    ASP B  46   CB  -  CG  -  OD1 ANGL. DEV. =   5.6 DEGREES          
REMARK 500    ASP B  65   CB  -  CG  -  OD1 ANGL. DEV. =   5.4 DEGREES          
REMARK 500    ASP B  65   CB  -  CG  -  OD2 ANGL. DEV. =  -6.0 DEGREES          
REMARK 500    ARG B  69   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500    ASP B  89   CB  -  CG  -  OD1 ANGL. DEV. =   5.8 DEGREES          
REMARK 500    ASP B  89   CB  -  CG  -  OD2 ANGL. DEV. =  -6.1 DEGREES          
REMARK 500    CYS B 104   N   -  CA  -  CB  ANGL. DEV. =   9.7 DEGREES          
REMARK 500    ARG B 133   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.9 DEGREES          
REMARK 500    ASP B 147   CB  -  CG  -  OD2 ANGL. DEV. =  -6.3 DEGREES          
REMARK 500    ARG B 191   NE  -  CZ  -  NH1 ANGL. DEV. =   5.6 DEGREES          
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      65 ANGLE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    LEU A   8       -7.41     77.69                                   
REMARK 500    ALA A  87       74.15    -63.61                                   
REMARK 500    GLU A  97       18.07    -68.50                                   
REMARK 500    SER A 278      -61.75   -107.56                                   
REMARK 500    ARG A 283      176.22    172.20                                   
REMARK 500    ASN A 334       59.53   -158.80                                   
REMARK 500    LEU B   8       -6.92     73.68                                   
REMARK 500    ALA B  85       56.32    -65.30                                   
REMARK 500    GLU B 123      -71.32    -85.90                                   
REMARK 500    MET B 158       60.90   -117.08                                   
REMARK 500    VAL B 197      130.01    -31.65                                   
REMARK 500    SER B 278      -68.76   -106.84                                   
REMARK 500    ARG B 283      168.99    178.25                                   
REMARK 500    ASN B 334       56.79   -154.73                                   
REMARK 500    ASP B 359       78.19   -151.18                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG A 394  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 AGS A   1   O2G                                                    
REMARK 620 2 AGS A   1   O2A 115.0                                              
REMARK 620 3 HOH A 467   O    78.8  93.8                                        
REMARK 620 4 GLU A 279   OE1 108.0  82.6 173.1                                  
REMARK 620 5 GLU A 267   OE2 148.7  96.0  95.5  79.2                            
REMARK 620 6 GLU A 267   OE1  92.7 152.3  90.0  90.6  56.3                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG A 393  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 AGS A   1   O3G                                                    
REMARK 620 2 AGS A   1   O2B  82.9                                              
REMARK 620 3 HOH A 736   O   132.5  96.8                                        
REMARK 620 4 GLU A 279   OE2 130.8  85.3  96.3                                  
REMARK 620 5 GLU A 279   OE1  84.9  80.7 142.3  46.0                            
REMARK 620 6 HOH A 627   O   102.3 159.0  94.5  75.8  79.5                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              NA A 395  NA                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 PRO A 103   O                                                      
REMARK 620 2 HOH A 432   O   123.1                                              
REMARK 620 3 ASN A 100   ND2 126.3  85.3                                        
REMARK 620 4 VAL A 101   O    73.7  73.6  73.0                                  
REMARK 620 N                    1     2     3                                   
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG B 393  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 HOH B 479   O                                                      
REMARK 620 2 GLU B 279   OE1  90.2                                              
REMARK 620 3 HOH B 626   O    88.2 155.4                                        
REMARK 620 4 AGS B 395   O2B 164.9 101.2  77.4                                  
REMARK 620 5 AGS B 395   O3G 113.1  96.3 106.9  75.7                            
REMARK 620 6 GLU B 279   OE2  71.3  59.7  96.6 105.9 156.0                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              MG B 394  MG                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 GLU B 267   OE1                                                    
REMARK 620 2 GLU B 267   OE2  56.1                                              
REMARK 620 3 HOH B 527   O    96.5  87.2                                        
REMARK 620 4 AGS B 395   O2G  84.4 135.3  76.5                                  
REMARK 620 5 AGS B 395   O2A 169.0 122.6  94.3 100.1                            
REMARK 620 6 GLU B 279   OE1  82.7 109.2 158.8  82.4  88.0                      
REMARK 620 N                    1     2     3     4     5                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG A 393                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG A 394                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC3                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG B 393                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC4                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MG B 394                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC5                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE NA A 395                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC6                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CL A 396                  
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC7                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE AGS A 1                   
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC8                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE AGS B 395                 
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC9                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE MPO A 397                 
DBREF  1KJJ A    2   392  UNP    P33221   PURT_ECOLI       1    391             
DBREF  1KJJ B    2   392  UNP    P33221   PURT_ECOLI       1    391             
SEQRES   1 A  391  THR LEU LEU GLY THR ALA LEU ARG PRO ALA ALA THR ARG          
SEQRES   2 A  391  VAL MET LEU LEU GLY SER GLY GLU LEU GLY LYS GLU VAL          
SEQRES   3 A  391  ALA ILE GLU CYS GLN ARG LEU GLY VAL GLU VAL ILE ALA          
SEQRES   4 A  391  VAL ASP ARG TYR ALA ASP ALA PRO ALA MET HIS VAL ALA          
SEQRES   5 A  391  HIS ARG SER HIS VAL ILE ASN MET LEU ASP GLY ASP ALA          
SEQRES   6 A  391  LEU ARG ARG VAL VAL GLU LEU GLU LYS PRO HIS TYR ILE          
SEQRES   7 A  391  VAL PRO GLU ILE GLU ALA ILE ALA THR ASP MET LEU ILE          
SEQRES   8 A  391  GLN LEU GLU GLU GLU GLY LEU ASN VAL VAL PRO CYS ALA          
SEQRES   9 A  391  ARG ALA THR LYS LEU THR MET ASN ARG GLU GLY ILE ARG          
SEQRES  10 A  391  ARG LEU ALA ALA GLU GLU LEU GLN LEU PRO THR SER THR          
SEQRES  11 A  391  TYR ARG PHE ALA ASP SER GLU SER LEU PHE ARG GLU ALA          
SEQRES  12 A  391  VAL ALA ASP ILE GLY TYR PRO CYS ILE VAL LYS PRO VAL          
SEQRES  13 A  391  MET SER SER SER GLY LYS GLY GLN THR PHE ILE ARG SER          
SEQRES  14 A  391  ALA GLU GLN LEU ALA GLN ALA TRP LYS TYR ALA GLN GLN          
SEQRES  15 A  391  GLY GLY ARG ALA GLY ALA GLY ARG VAL ILE VAL GLU GLY          
SEQRES  16 A  391  VAL VAL LYS PHE ASP PHE GLU ILE THR LEU LEU THR VAL          
SEQRES  17 A  391  SER ALA VAL ASP GLY VAL HIS PHE CYS ALA PRO VAL GLY          
SEQRES  18 A  391  HIS ARG GLN GLU ASP GLY ASP TYR ARG GLU SER TRP GLN          
SEQRES  19 A  391  PRO GLN GLN MET SER PRO LEU ALA LEU GLU ARG ALA GLN          
SEQRES  20 A  391  GLU ILE ALA ARG LYS VAL VAL LEU ALA LEU GLY GLY TYR          
SEQRES  21 A  391  GLY LEU PHE GLY VAL GLU LEU PHE VAL CYS GLY ASP GLU          
SEQRES  22 A  391  VAL ILE PHE SER GLU VAL SER PRO ARG PRO HIS ASP THR          
SEQRES  23 A  391  GLY MET VAL THR LEU ILE SER GLN ASP LEU SER GLU PHE          
SEQRES  24 A  391  ALA LEU HIS VAL ARG ALA PHE LEU GLY LEU PRO VAL GLY          
SEQRES  25 A  391  GLY ILE ARG GLN TYR GLY PRO ALA ALA SER ALA VAL ILE          
SEQRES  26 A  391  LEU PRO GLN LEU THR SER GLN ASN VAL THR PHE ASP ASN          
SEQRES  27 A  391  VAL GLN ASN ALA VAL GLY ALA ASP LEU GLN ILE ARG LEU          
SEQRES  28 A  391  PHE GLY LYS PRO GLU ILE ASP GLY SER ARG ARG LEU GLY          
SEQRES  29 A  391  VAL ALA LEU ALA THR ALA GLU SER VAL VAL ASP ALA ILE          
SEQRES  30 A  391  GLU ARG ALA LYS HIS ALA ALA GLY GLN VAL LYS VAL GLN          
SEQRES  31 A  391  GLY                                                          
SEQRES   1 B  391  THR LEU LEU GLY THR ALA LEU ARG PRO ALA ALA THR ARG          
SEQRES   2 B  391  VAL MET LEU LEU GLY SER GLY GLU LEU GLY LYS GLU VAL          
SEQRES   3 B  391  ALA ILE GLU CYS GLN ARG LEU GLY VAL GLU VAL ILE ALA          
SEQRES   4 B  391  VAL ASP ARG TYR ALA ASP ALA PRO ALA MET HIS VAL ALA          
SEQRES   5 B  391  HIS ARG SER HIS VAL ILE ASN MET LEU ASP GLY ASP ALA          
SEQRES   6 B  391  LEU ARG ARG VAL VAL GLU LEU GLU LYS PRO HIS TYR ILE          
SEQRES   7 B  391  VAL PRO GLU ILE GLU ALA ILE ALA THR ASP MET LEU ILE          
SEQRES   8 B  391  GLN LEU GLU GLU GLU GLY LEU ASN VAL VAL PRO CYS ALA          
SEQRES   9 B  391  ARG ALA THR LYS LEU THR MET ASN ARG GLU GLY ILE ARG          
SEQRES  10 B  391  ARG LEU ALA ALA GLU GLU LEU GLN LEU PRO THR SER THR          
SEQRES  11 B  391  TYR ARG PHE ALA ASP SER GLU SER LEU PHE ARG GLU ALA          
SEQRES  12 B  391  VAL ALA ASP ILE GLY TYR PRO CYS ILE VAL LYS PRO VAL          
SEQRES  13 B  391  MET SER SER SER GLY LYS GLY GLN THR PHE ILE ARG SER          
SEQRES  14 B  391  ALA GLU GLN LEU ALA GLN ALA TRP LYS TYR ALA GLN GLN          
SEQRES  15 B  391  GLY GLY ARG ALA GLY ALA GLY ARG VAL ILE VAL GLU GLY          
SEQRES  16 B  391  VAL VAL LYS PHE ASP PHE GLU ILE THR LEU LEU THR VAL          
SEQRES  17 B  391  SER ALA VAL ASP GLY VAL HIS PHE CYS ALA PRO VAL GLY          
SEQRES  18 B  391  HIS ARG GLN GLU ASP GLY ASP TYR ARG GLU SER TRP GLN          
SEQRES  19 B  391  PRO GLN GLN MET SER PRO LEU ALA LEU GLU ARG ALA GLN          
SEQRES  20 B  391  GLU ILE ALA ARG LYS VAL VAL LEU ALA LEU GLY GLY TYR          
SEQRES  21 B  391  GLY LEU PHE GLY VAL GLU LEU PHE VAL CYS GLY ASP GLU          
SEQRES  22 B  391  VAL ILE PHE SER GLU VAL SER PRO ARG PRO HIS ASP THR          
SEQRES  23 B  391  GLY MET VAL THR LEU ILE SER GLN ASP LEU SER GLU PHE          
SEQRES  24 B  391  ALA LEU HIS VAL ARG ALA PHE LEU GLY LEU PRO VAL GLY          
SEQRES  25 B  391  GLY ILE ARG GLN TYR GLY PRO ALA ALA SER ALA VAL ILE          
SEQRES  26 B  391  LEU PRO GLN LEU THR SER GLN ASN VAL THR PHE ASP ASN          
SEQRES  27 B  391  VAL GLN ASN ALA VAL GLY ALA ASP LEU GLN ILE ARG LEU          
SEQRES  28 B  391  PHE GLY LYS PRO GLU ILE ASP GLY SER ARG ARG LEU GLY          
SEQRES  29 B  391  VAL ALA LEU ALA THR ALA GLU SER VAL VAL ASP ALA ILE          
SEQRES  30 B  391  GLU ARG ALA LYS HIS ALA ALA GLY GLN VAL LYS VAL GLN          
SEQRES  31 B  391  GLY                                                          
HET     MG  A 393       1                                                       
HET     MG  A 394       1                                                       
HET     NA  A 395       1                                                       
HET     CL  A 396       1                                                       
HET    AGS  A   1      31                                                       
HET    MPO  A 397      13                                                       
HET     MG  B 393       1                                                       
HET     MG  B 394       1                                                       
HET    AGS  B 395      31                                                       
HETNAM      MG MAGNESIUM ION                                                    
HETNAM      NA SODIUM ION                                                       
HETNAM      CL CHLORIDE ION                                                     
HETNAM     AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER                       
HETNAM     MPO 3[N-MORPHOLINO]PROPANE SULFONIC ACID                             
HETSYN     AGS ATP-GAMMA-S; ADENOSINE 5'-(3-THIOTRIPHOSPHATE);                  
HETSYN   2 AGS  ADENOSINE 5'-(GAMMA-THIOTRIPHOSPHATE); ADENOSINE-5'-            
HETSYN   3 AGS  DIPHOSPHATE MONOTHIOPHOSPHATE                                   
FORMUL   3   MG    4(MG 2+)                                                     
FORMUL   5   NA    NA 1+                                                        
FORMUL   6   CL    CL 1-                                                        
FORMUL   7  AGS    2(C10 H16 N5 O12 P3 S)                                       
FORMUL   8  MPO    C7 H15 N O4 S                                                
FORMUL  12  HOH   *674(H2 O)                                                    
HELIX    1   1 GLY A   21  ARG A   33  1                                  13    
HELIX    2   2 ALA A   47  ALA A   53  5                                   7    
HELIX    3   3 ASP A   63  LYS A   75  1                                  13    
HELIX    4   4 ALA A   87  GLU A   97  1                                  11    
HELIX    5   5 CYS A  104  MET A  112  1                                   9    
HELIX    6   6 ASN A  113  GLU A  123  1                                  11    
HELIX    7   7 SER A  137  GLY A  149  1                                  13    
HELIX    8   8 SER A  170  GLU A  172  5                                   3    
HELIX    9   9 GLN A  173  GLY A  184  1                                  12    
HELIX   10  10 SER A  240  GLY A  259  1                                  20    
HELIX   11  11 HIS A  285  MET A  289  5                                   5    
HELIX   12  12 VAL A  290  GLN A  295  1                                   6    
HELIX   13  13 SER A  298  LEU A  308  1                                  11    
HELIX   14  14 ASN A  339  ALA A  343  5                                   5    
HELIX   15  15 SER A  373  VAL A  388  1                                  16    
HELIX   16  16 GLY B   21  LEU B   34  1                                  14    
HELIX   17  17 ALA B   47  VAL B   52  1                                   6    
HELIX   18  18 ASP B   63  LYS B   75  1                                  13    
HELIX   19  19 ALA B   87  GLU B   97  1                                  11    
HELIX   20  20 CYS B  104  ASN B  113  1                                  10    
HELIX   21  21 ASN B  113  GLU B  123  1                                  11    
HELIX   22  22 SER B  137  GLY B  149  1                                  13    
HELIX   23  23 GLN B  173  ALA B  181  1                                   9    
HELIX   24  24 SER B  240  GLY B  259  1                                  20    
HELIX   25  25 HIS B  285  MET B  289  5                                   5    
HELIX   26  26 VAL B  290  GLN B  295  1                                   6    
HELIX   27  27 SER B  298  LEU B  308  1                                  11    
HELIX   28  28 ASN B  339  ALA B  343  5                                   5    
HELIX   29  29 SER B  373  VAL B  388  1                                  16    
SHEET    1   A 5 ARG A  55  VAL A  58  0                                        
SHEET    2   A 5 GLU A  37  ASP A  42  1  N  ALA A  40   O  ARG A  55           
SHEET    3   A 5 ARG A  14  LEU A  18  1  N  VAL A  15   O  GLU A  37           
SHEET    4   A 5 TYR A  78  PRO A  81  1  O  VAL A  80   N  MET A  16           
SHEET    5   A 5 ASN A 100  VAL A 101  1  O  ASN A 100   N  ILE A  79           
SHEET    1   B 4 TYR A 132  ALA A 135  0                                        
SHEET    2   B 4 VAL A 192  GLY A 196 -1  O  VAL A 194   N  ARG A 133           
SHEET    3   B 4 CYS A 152  PRO A 156 -1  N  ILE A 153   O  GLU A 195           
SHEET    4   B 4 THR A 166  ILE A 168 -1  O  ILE A 168   N  CYS A 152           
SHEET    1   C 4 GLU A 274  SER A 281  0                                        
SHEET    2   C 4 GLY A 262  CYS A 271 -1  N  PHE A 269   O  ILE A 276           
SHEET    3   C 4 PHE A 202  ALA A 211 -1  N  THR A 208   O  PHE A 264           
SHEET    4   C 4 GLY A 214  PHE A 217 -1  O  GLY A 214   N  ALA A 211           
SHEET    1   D 8 GLU A 274  SER A 281  0                                        
SHEET    2   D 8 GLY A 262  CYS A 271 -1  N  PHE A 269   O  ILE A 276           
SHEET    3   D 8 PHE A 202  ALA A 211 -1  N  THR A 208   O  PHE A 264           
SHEET    4   D 8 VAL A 221  GLU A 226 -1  O  VAL A 221   N  THR A 205           
SHEET    5   D 8 ASP A 229  GLN A 235 -1  O  ARG A 231   N  ARG A 224           
SHEET    6   D 8 ALA A 321  ILE A 326 -1  O  ALA A 321   N  GLN A 235           
SHEET    7   D 8 GLY A 365  THR A 370 -1  O  ALA A 367   N  ALA A 324           
SHEET    8   D 8 LEU A 348  LEU A 352 -1  N  GLN A 349   O  LEU A 368           
SHEET    1   E 2 GLN A 329  SER A 332  0                                        
SHEET    2   E 2 ILE A 358  SER A 361 -1  O  GLY A 360   N  LEU A 330           
SHEET    1   F 2 THR A 336  ASP A 338  0                                        
SHEET    2   F 2 LYS A 389  GLN A 391 -1  O  GLN A 391   N  THR A 336           
SHEET    1   G 4 ARG B  55  VAL B  58  0                                        
SHEET    2   G 4 GLU B  37  ASP B  42  1  N  ALA B  40   O  ARG B  55           
SHEET    3   G 4 ARG B  14  LEU B  18  1  N  VAL B  15   O  GLU B  37           
SHEET    4   G 4 TYR B  78  PRO B  81  1  O  VAL B  80   N  MET B  16           
SHEET    1   H 4 THR B 131  ALA B 135  0                                        
SHEET    2   H 4 VAL B 192  GLY B 196 -1  O  VAL B 194   N  ARG B 133           
SHEET    3   H 4 CYS B 152  PRO B 156 -1  N  ILE B 153   O  GLU B 195           
SHEET    4   H 4 THR B 166  ILE B 168 -1  O  ILE B 168   N  CYS B 152           
SHEET    1   I 4 GLU B 274  SER B 281  0                                        
SHEET    2   I 4 GLY B 262  CYS B 271 -1  N  GLY B 265   O  SER B 281           
SHEET    3   I 4 PHE B 202  ALA B 211 -1  N  LEU B 206   O  VAL B 266           
SHEET    4   I 4 GLY B 214  PHE B 217 -1  O  HIS B 216   N  VAL B 209           
SHEET    1   J 8 GLU B 274  SER B 281  0                                        
SHEET    2   J 8 GLY B 262  CYS B 271 -1  N  GLY B 265   O  SER B 281           
SHEET    3   J 8 PHE B 202  ALA B 211 -1  N  LEU B 206   O  VAL B 266           
SHEET    4   J 8 VAL B 221  GLU B 226 -1  O  VAL B 221   N  THR B 205           
SHEET    5   J 8 ASP B 229  GLN B 235 -1  O  ARG B 231   N  ARG B 224           
SHEET    6   J 8 ALA B 321  ILE B 326 -1  O  ALA B 321   N  GLN B 235           
SHEET    7   J 8 GLY B 365  THR B 370 -1  O  ALA B 367   N  ALA B 324           
SHEET    8   J 8 LEU B 348  LEU B 352 -1  N  GLN B 349   O  LEU B 368           
SHEET    1   K 2 GLN B 329  SER B 332  0                                        
SHEET    2   K 2 ILE B 358  SER B 361 -1  O  GLY B 360   N  LEU B 330           
SHEET    1   L 2 THR B 336  ASP B 338  0                                        
SHEET    2   L 2 LYS B 389  GLN B 391 -1  O  LYS B 389   N  ASP B 338           
LINK         O2G AGS A   1                MG    MG A 394     1555   1555  1.79  
LINK         O3G AGS A   1                MG    MG A 393     1555   1555  1.73  
LINK         O2B AGS A   1                MG    MG A 393     1555   1555  2.04  
LINK         O2A AGS A   1                MG    MG A 394     1555   1555  1.96  
LINK        MG    MG A 393                 O   HOH A 736     1555   1555  1.91  
LINK        MG    MG A 393                 OE2 GLU A 279     1555   1555  2.95  
LINK        MG    MG A 393                 OE1 GLU A 279     1555   1555  2.76  
LINK        MG    MG A 393                 O   HOH A 627     1555   1555  2.46  
LINK        MG    MG A 394                 O   HOH A 467     1555   1555  2.06  
LINK        MG    MG A 394                 OE1 GLU A 279     1555   1555  2.10  
LINK        MG    MG A 394                 OE2 GLU A 267     1555   1555  2.48  
LINK        MG    MG A 394                 OE1 GLU A 267     1555   1555  2.13  
LINK        NA    NA A 395                 O   PRO A 103     1555   1555  2.46  
LINK        NA    NA A 395                 O   HOH A 432     1555   1555  2.50  
LINK        MG    MG B 393                 O   HOH B 479     1555   1555  2.84  
LINK        MG    MG B 393                 OE1 GLU B 279     1555   1555  2.11  
LINK        MG    MG B 393                 O   HOH B 626     1555   1555  1.68  
LINK        MG    MG B 393                 O2B AGS B 395     1555   1555  2.13  
LINK        MG    MG B 393                 O3G AGS B 395     1555   1555  2.28  
LINK        MG    MG B 393                 OE2 GLU B 279     1555   1555  2.34  
LINK        MG    MG B 394                 OE1 GLU B 267     1555   1555  2.62  
LINK        MG    MG B 394                 OE2 GLU B 267     1555   1555  1.78  
LINK        MG    MG B 394                 O   HOH B 527     1555   1555  2.45  
LINK        MG    MG B 394                 O2G AGS B 395     1555   1555  1.97  
LINK        MG    MG B 394                 O2A AGS B 395     1555   1555  1.95  
LINK        MG    MG B 394                 OE1 GLU B 279     1555   1555  2.04  
LINK         ND2 ASN A 100                NA    NA A 395     1555   1555  2.78  
LINK         O   VAL A 101                NA    NA A 395     1555   1555  2.69  
CISPEP   1 VAL A  102    PRO A  103          0        -4.39                     
CISPEP   2 TYR A  150    PRO A  151          0        -2.90                     
CISPEP   3 GLN A  235    PRO A  236          0        -4.33                     
CISPEP   4 VAL B  102    PRO B  103          0        -0.69                     
CISPEP   5 TYR B  150    PRO B  151          0         0.19                     
CISPEP   6 GLN B  235    PRO B  236          0         0.08                     
SITE     1 AC1  4 AGS A   1  GLU A 279  HOH A 627  HOH A 736                    
SITE     1 AC2  4 AGS A   1  GLU A 267  GLU A 279  HOH A 467                    
SITE     1 AC3  4 GLU B 279  AGS B 395  HOH B 479  HOH B 626                    
SITE     1 AC4  4 GLU B 267  GLU B 279  AGS B 395  HOH B 527                    
SITE     1 AC5  4 ASN A 100  VAL A 101  PRO A 103  HOH A 432                    
SITE     1 AC6  5 ARG A  43  VAL A  58  ARG A 119  PHE A 134                    
SITE     2 AC6  5 HOH A 741                                                     
SITE     1 AC7 26 ARG A 114  SER A 130  ILE A 153  LYS A 155                    
SITE     2 AC7 26 SER A 160  SER A 161  GLY A 162  GLN A 165                    
SITE     3 AC7 26 GLU A 195  GLY A 196  VAL A 198  GLU A 203                    
SITE     4 AC7 26 GLN A 225  GLU A 267  PHE A 269  GLU A 279                    
SITE     5 AC7 26  MG A 393   MG A 394  HOH A 467  HOH A 471                    
SITE     6 AC7 26 HOH A 495  HOH A 497  HOH A 514  HOH A 617                    
SITE     7 AC7 26 HOH A 702  HOH A 736                                          
SITE     1 AC8 18 ARG B 114  LYS B 155  SER B 159  SER B 160                    
SITE     2 AC8 18 SER B 161  GLY B 162  GLN B 165  GLU B 195                    
SITE     3 AC8 18 VAL B 197  VAL B 198  GLU B 203  GLU B 267                    
SITE     4 AC8 18 GLU B 279   MG B 393   MG B 394  HOH B 461                    
SITE     5 AC8 18 HOH B 527  HOH B 626                                          
SITE     1 AC9 11 ASP A  46  MET A  50  HIS A  51  ARG A  55                    
SITE     2 AC9 11 SER A  56  GLU A 143  HOH A 402  HOH A 465                    
SITE     3 AC9 11 HOH A 523  LEU B   8  HIS B  54                               
CRYST1   61.900  179.300   76.100  90.00  90.00  90.00 P 21 21 2     8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016155  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.005577  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.013141        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system