HEADER TRANSCRIPTION 04-JAN-02 1KQ8
TITLE SOLUTION STRUCTURE OF WINGED HELIX PROTEIN HFH-1
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: HEPATOCYTE NUCLEAR FACTOR 3 FORKHEAD HOMOLOG 1;
COMPND 3 CHAIN: A;
COMPND 4 SYNONYM: HFH-1; HNF-3/FORKHEAD HOMOLOG-1; WINGED HELIX/FORKHEAD
COMPND 5 TRANSCRIPTION FACTOR;
COMPND 6 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;
SOURCE 3 ORGANISM_COMMON: NORWAY RAT;
SOURCE 4 ORGANISM_TAXID: 10116;
SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 6 EXPRESSION_SYSTEM_TAXID: 562
KEYWDS WINGED HELIX PROTEIN, HFH-1, TRANSCRIPTION
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR W.SHENG,M.RANCE,X.LIAO
REVDAT 6 23-FEB-22 1KQ8 1 REMARK SEQADV
REVDAT 5 24-FEB-09 1KQ8 1 VERSN
REVDAT 4 25-FEB-03 1KQ8 1 REMARK
REVDAT 3 18-MAR-02 1KQ8 1 JRNL
REVDAT 2 22-FEB-02 1KQ8 1 AUTHOR JRNL
REVDAT 1 22-JAN-02 1KQ8 0
JRNL AUTH W.SHENG,M.RANCE,X.LIAO
JRNL TITL STRUCTURE COMPARISON OF TWO CONSERVED HNF-3/FKH PROTEINS
JRNL TITL 2 HFH-1 AND GENESIS INDICATES THE EXISTENCE OF FOLDING
JRNL TITL 3 DIFFERENCES IN THEIR COMPLEXES WITH A DNA BINDING SEQUENCE.
JRNL REF BIOCHEMISTRY V. 41 3286 2002
JRNL REFN ISSN 0006-2960
JRNL PMID 11876636
JRNL DOI 10.1021/BI011908K
REMARK 1
REMARK 1 REFERENCE 1
REMARK 1 AUTH I.MARSDEN,C.JIN,X.LIAO
REMARK 1 TITL STRUCTURAL CHANGES IN THE REGION DIRECTLY ADJACENT TO THE
REMARK 1 TITL 2 DNA-BINDING HELIX HIGHLIGHT A POSSIBLE MECHANISM TO EXPLAIN
REMARK 1 TITL 3 THE OBSERVED CHANGES IN THE SEQUENCE-SPECIFIC BINDING OF
REMARK 1 TITL 4 WINGED HELIX PROTEINS
REMARK 1 REF J.MOL.BIOL. V. 278 293 1998
REMARK 1 REFN ISSN 0022-2836
REMARK 1 DOI 10.1006/JMBI.1998.1703
REMARK 1 REFERENCE 2
REMARK 1 AUTH C.JIN,I.MARSDEN,X.CHEN,X.LIAO
REMARK 1 TITL SEQUENCE SPECIFIC COLLECTIVE MOTIONS IN A WINGED HELIX DNA
REMARK 1 TITL 2 BINDING DOMAIN DETECTED BY 15N RELAXATION NMR
REMARK 1 REF BIOCHEMISTRY V. 37 6179 1998
REMARK 1 REFN ISSN 0006-2960
REMARK 1 DOI 10.1021/BI980031V
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : DYANA 1.5
REMARK 3 AUTHORS : GUNTER, P.
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 1KQ8 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-JAN-02.
REMARK 100 THE DEPOSITION ID IS D_1000015223.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 290
REMARK 210 PH : 6.5
REMARK 210 IONIC STRENGTH : NULL
REMARK 210 PRESSURE : NULL
REMARK 210 SAMPLE CONTENTS : NULL
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 3D_13C
REMARK 210 -SEPARATED_NOESY; HNCA-J; HNHA
REMARK 210 SPECTROMETER FIELD STRENGTH : 600 MHZ; 750 MHZ; 800 MHZ
REMARK 210 SPECTROMETER MODEL : DRX; DMX; UNITYPLUS
REMARK 210 SPECTROMETER MANUFACTURER : BRUKER; VARIAN
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : NULL
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 200
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : TARGET FUNCTION
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL
REMARK 210
REMARK 210 REMARK: NULL
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 465 SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465 MODELS 1-20
REMARK 465 RES C SSSEQI
REMARK 465 PRO A 2
REMARK 465 LYS A 3
REMARK 465 PRO A 4
REMARK 465 PRO A 5
REMARK 465 TYR A 6
REMARK 465 SER A 7
REMARK 465 ASN A 83
REMARK 465 SER A 84
REMARK 465 GLU A 85
REMARK 465 TYR A 86
REMARK 465 THR A 87
REMARK 465 PHE A 88
REMARK 465 ALA A 89
REMARK 465 ASP A 90
REMARK 465 GLY A 91
REMARK 465 VAL A 92
REMARK 465 PHE A 93
REMARK 465 ARG A 94
REMARK 465 ARG A 95
REMARK 465 ARG A 96
REMARK 465 ARG A 97
REMARK 465 TYR A 98
REMARK 465 ARG A 99
REMARK 465 LEU A 100
REMARK 465 SER A 101
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: CLOSE CONTACTS
REMARK 500
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.
REMARK 500
REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE
REMARK 500 O LEU A 24 H TRP A 78 1.47
REMARK 500 O TRP A 47 H VAL A 51 1.51
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 SER A 19 -142.05 -89.09
REMARK 500 1 ALA A 20 33.61 -168.50
REMARK 500 1 SER A 43 -69.55 -143.50
REMARK 500 1 TYR A 44 107.99 -169.93
REMARK 500 1 ASN A 58 -76.16 -108.43
REMARK 500 1 CYS A 60 59.77 88.93
REMARK 500 1 PHE A 61 -169.52 -119.19
REMARK 500 1 ARG A 66 89.01 40.34
REMARK 500 1 SER A 69 65.74 -177.59
REMARK 500 1 TRP A 72 -36.89 91.33
REMARK 500 1 LYS A 74 51.20 177.30
REMARK 500 2 SER A 19 -144.33 -86.23
REMARK 500 2 ALA A 20 31.68 -166.07
REMARK 500 2 SER A 43 -79.93 -135.47
REMARK 500 2 TYR A 44 119.44 -165.65
REMARK 500 2 ASN A 58 -88.93 -99.21
REMARK 500 2 CYS A 60 64.47 92.46
REMARK 500 2 PHE A 61 -165.33 -119.22
REMARK 500 2 ARG A 66 95.42 49.01
REMARK 500 2 SER A 69 58.05 -176.32
REMARK 500 2 ARG A 70 65.35 -170.45
REMARK 500 2 TRP A 72 25.16 48.63
REMARK 500 2 LYS A 74 42.45 -161.57
REMARK 500 2 ASP A 75 -68.85 -175.61
REMARK 500 3 SER A 19 -143.28 -82.79
REMARK 500 3 ALA A 20 32.91 -169.32
REMARK 500 3 SER A 43 -72.97 -150.30
REMARK 500 3 TYR A 44 117.65 -174.81
REMARK 500 3 ASN A 58 -89.84 -98.39
REMARK 500 3 CYS A 60 63.88 91.40
REMARK 500 3 PHE A 61 -165.33 -119.73
REMARK 500 3 SER A 69 41.04 177.50
REMARK 500 3 ARG A 70 68.52 -171.57
REMARK 500 3 TRP A 72 -38.58 80.63
REMARK 500 3 LYS A 74 -40.74 -173.24
REMARK 500 3 ASN A 81 75.97 -167.49
REMARK 500 4 ALA A 20 43.24 82.19
REMARK 500 4 SER A 43 -76.82 -69.90
REMARK 500 4 THR A 45 48.67 -94.02
REMARK 500 4 ASN A 58 -80.97 -93.06
REMARK 500 4 CYS A 60 78.90 95.77
REMARK 500 4 PHE A 61 -166.52 -123.96
REMARK 500 4 ARG A 66 75.80 -154.97
REMARK 500 4 SER A 69 41.81 -167.25
REMARK 500 4 ARG A 70 67.31 -165.32
REMARK 500 4 TRP A 72 -40.06 -178.52
REMARK 500 4 LYS A 74 -40.16 -173.34
REMARK 500 5 SER A 19 -149.71 -86.19
REMARK 500 5 ALA A 20 23.18 -154.45
REMARK 500 5 SER A 43 -74.80 -69.48
REMARK 500
REMARK 500 THIS ENTRY HAS 217 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
DBREF 1KQ8 A 2 101 UNP Q63244 FOXQ1_RAT 99 198
SEQADV 1KQ8 THR A 13 UNP Q63244 ALA 110 CONFLICT
SEQADV 1KQ8 TYR A 98 UNP Q63244 LYS 195 CONFLICT
SEQRES 1 A 100 PRO LYS PRO PRO TYR SER TYR ILE ALA LEU ILE THR MET
SEQRES 2 A 100 ALA ILE ARG ASP SER ALA GLY GLY ARG LEU THR LEU ALA
SEQRES 3 A 100 GLU ILE ASN GLU TYR LEU MET GLY LYS PHE PRO PHE PHE
SEQRES 4 A 100 ARG GLY SER TYR THR GLY TRP ARG ASN SER VAL ARG HIS
SEQRES 5 A 100 ASN LEU SER LEU ASN ASP CYS PHE VAL LYS VAL LEU ARG
SEQRES 6 A 100 ASP PRO SER ARG PRO TRP GLY LYS ASP ASN TYR TRP MET
SEQRES 7 A 100 LEU ASN PRO ASN SER GLU TYR THR PHE ALA ASP GLY VAL
SEQRES 8 A 100 PHE ARG ARG ARG ARG TYR ARG LEU SER
HELIX 1 1 TYR A 8 ARG A 17 1 10
HELIX 2 2 THR A 25 PHE A 37 1 13
HELIX 3 3 PHE A 39 SER A 43 5 5
HELIX 4 4 TRP A 47 ASP A 59 1 13
SHEET 1 A 2 VAL A 62 LYS A 63 0
SHEET 2 A 2 TRP A 78 MET A 79 -1 O MET A 79 N VAL A 62
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
