HEADER SIGNALING PROTEIN 05-AUG-02 1MC7
TITLE SOLUTION STRUCTURE OF MDVL1 PDZ DOMAIN
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: SEGMENT POLARITY PROTEIN DISHEVELLED HOMOLOG DVL-
COMPND 3 1;
COMPND 4 CHAIN: A;
COMPND 5 FRAGMENT: PDZ DOMAIN;
COMPND 6 SYNONYM: DISHEVELLED-1, DSH HOMOLOG 1;
COMPND 7 ENGINEERED: YES;
COMPND 8 MUTATION: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;
SOURCE 3 ORGANISM_COMMON: HOUSE MOUSE;
SOURCE 4 ORGANISM_TAXID: 10090;
SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;
SOURCE 6 EXPRESSION_SYSTEM_TAXID: 511693;
SOURCE 7 EXPRESSION_SYSTEM_STRAIN: BL-21;
SOURCE 8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PET28
KEYWDS PDZ DOMAIN, WNT SIGNALING, SIGNALING PROTEIN
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR H.-C.WONG,A.BOURDELAS,Y.SHAO,D.WU,D.L.SHI,J.ZHENG
REVDAT 3 24-FEB-09 1MC7 1 VERSN
REVDAT 2 16-DEC-03 1MC7 1 JRNL
REVDAT 1 23-SEP-03 1MC7 0
JRNL AUTH H.-C.WONG,A.BOURDELAS,A.KRAUSS,H.-J.LEE,Y.SHAO,
JRNL AUTH 2 D.WU,M.MLODZIK,D.-L.SHI,J.ZHENG
JRNL TITL DIRECT BINDING OF THE PDZ DOMAIN OF DISHEVELLED TO
JRNL TITL 2 A CONSERVED INTERNAL SEQUENCE IN THE C-TERMINAL
JRNL TITL 3 REGION OF FRIZZLED
JRNL REF MOL.CELL V. 12 1251 2003
JRNL REFN ISSN 1097-2765
JRNL PMID 14636582
JRNL DOI 10.1016/S1097-2765(03)00427-1
REMARK 1
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : DYANA
REMARK 3 AUTHORS : GUNTERT
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON A TOTAL
REMARK 3 OF 1190 RESTRAINTS: 1124 ARE NOE-DERIVED DISTANCE CONSTRAINTS,
REMARK 3 44 ARE DIHEDRAL ANGLE RESTRAINTS AND 22 ARE DISTANCE
REMARK 3 RESTRAINTS FROM HYDROGEN BONDS.
REMARK 4
REMARK 4 1MC7 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-AUG-02.
REMARK 100 THE RCSB ID CODE IS RCSB016817.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 298
REMARK 210 PH : 7.5
REMARK 210 IONIC STRENGTH : NULL
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1MM MDVL1 PDZ DOMAIN U-15N,
REMARK 210 13C, 100MM PHOSPHATE BUFFER PH
REMARK 210 7.5, 90% H2O, 10% D2O
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_13C-SEPARATED_NOESY, 3D_
REMARK 210 15N-SEPARATED_NOESY
REMARK 210 SPECTROMETER FIELD STRENGTH : 600 MHZ
REMARK 210 SPECTROMETER MODEL : INOVA
REMARK 210 SPECTROMETER MANUFACTURER : VARIAN
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : DYANA, NMRPIPE, XEASY
REMARK 210 METHOD USED : TORSION ANGLE DYNAMICS
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 320
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : TARGET FUNCTION
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1
REMARK 210
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR
REMARK 210 SPECTROSCOPY.
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 MET A 6 125.78 60.55
REMARK 500 1 GLU A 7 -47.31 -158.26
REMARK 500 1 HIS A 9 -60.95 78.83
REMARK 500 1 HIS A 10 173.35 -48.35
REMARK 500 1 GLN A 19 -64.60 -164.44
REMARK 500 1 SER A 20 140.81 164.82
REMARK 500 1 ASP A 22 -51.42 -175.12
REMARK 500 1 ARG A 23 115.90 -162.14
REMARK 500 1 ASP A 25 122.22 162.51
REMARK 500 1 ALA A 38 -88.34 -85.21
REMARK 500 1 ILE A 45 163.08 -41.85
REMARK 500 1 MET A 50 48.40 79.56
REMARK 500 1 ASN A 55 -141.31 39.84
REMARK 500 1 MET A 62 -83.15 -61.92
REMARK 500 1 SER A 63 54.03 36.60
REMARK 500 1 ASP A 65 25.26 46.04
REMARK 500 1 ASP A 66 29.46 -162.87
REMARK 500 1 ILE A 74 -91.76 -68.92
REMARK 500 1 VAL A 75 -37.33 -36.35
REMARK 500 1 GLN A 77 -134.05 -133.16
REMARK 500 1 THR A 78 160.22 -41.06
REMARK 500 1 SER A 82 45.65 156.85
REMARK 500 1 LEU A 83 146.30 -32.34
REMARK 500 1 ALA A 86 42.17 -92.75
REMARK 500 1 LYS A 87 171.16 54.22
REMARK 500 1 ALA A 88 118.76 179.50
REMARK 500 1 TRP A 89 101.64 -43.11
REMARK 500 1 ARG A 94 52.47 28.26
REMARK 500 2 MET A 6 170.67 60.88
REMARK 500 2 ARG A 8 94.09 43.86
REMARK 500 2 HIS A 9 105.39 -177.11
REMARK 500 2 LEU A 12 147.02 73.51
REMARK 500 2 GLN A 19 -55.68 -173.85
REMARK 500 2 SER A 20 89.57 44.72
REMARK 500 2 ASP A 25 90.27 45.44
REMARK 500 2 SER A 32 161.93 179.93
REMARK 500 2 MET A 34 83.13 -57.44
REMARK 500 2 LYS A 35 144.55 -39.28
REMARK 500 2 ALA A 38 -74.71 -86.34
REMARK 500 2 ARG A 44 54.55 35.33
REMARK 500 2 ILE A 45 164.90 -43.30
REMARK 500 2 MET A 50 50.72 80.78
REMARK 500 2 ASN A 55 -141.15 38.52
REMARK 500 2 GLU A 60 -75.48 -65.01
REMARK 500 2 MET A 62 -82.69 -56.69
REMARK 500 2 SER A 63 49.95 33.64
REMARK 500 2 ASP A 65 30.94 38.85
REMARK 500 2 ASP A 66 31.25 -166.62
REMARK 500 2 ILE A 74 -92.60 -66.71
REMARK 500 2 VAL A 75 -38.70 -38.58
REMARK 500 2 GLN A 77 -118.00 -133.22
REMARK 500 2 THR A 78 -173.26 -52.40
REMARK 500 2 SER A 82 51.76 152.91
REMARK 500 2 LEU A 83 152.23 -35.37
REMARK 500 2 LYS A 87 173.70 53.17
REMARK 500 2 ALA A 88 107.14 179.90
REMARK 500 2 TRP A 89 95.70 -59.38
REMARK 500 2 THR A 92 75.48 32.21
REMARK 500 3 GLU A 7 -54.32 176.76
REMARK 500 3 ARG A 8 86.78 62.67
REMARK 500 3 HIS A 10 -63.82 -92.37
REMARK 500 3 PHE A 11 150.67 -44.81
REMARK 500 3 LEU A 12 -74.96 -179.09
REMARK 500 3 GLN A 19 48.77 179.27
REMARK 500 3 SER A 20 94.82 -68.54
REMARK 500 3 ASP A 25 93.09 49.98
REMARK 500 3 SER A 32 166.78 180.00
REMARK 500 3 ALA A 38 -84.83 -82.40
REMARK 500 3 ARG A 44 63.27 38.59
REMARK 500 3 ILE A 45 165.15 -44.44
REMARK 500 3 MET A 50 49.79 78.00
REMARK 500 3 ASN A 55 -138.45 37.68
REMARK 500 3 ASN A 58 88.56 -150.46
REMARK 500 3 MET A 62 -83.94 -73.19
REMARK 500 3 SER A 63 57.63 34.63
REMARK 500 3 ASP A 66 30.56 -166.24
REMARK 500 3 ILE A 74 -91.76 -66.86
REMARK 500 3 VAL A 75 -37.75 -37.26
REMARK 500 3 GLN A 77 -132.66 -131.81
REMARK 500 3 THR A 78 159.46 -41.40
REMARK 500 3 SER A 82 48.13 153.15
REMARK 500 3 LEU A 83 151.36 -35.65
REMARK 500 3 ALA A 86 40.63 -91.25
REMARK 500 3 LYS A 87 172.80 53.17
REMARK 500 3 ALA A 88 114.83 -179.16
REMARK 500 3 TRP A 89 105.76 -42.66
REMARK 500 3 ASP A 90 118.49 178.39
REMARK 500 3 PRO A 91 -163.66 -75.04
REMARK 500 3 ARG A 94 61.04 37.00
REMARK 500 4 LEU A 4 178.92 -57.36
REMARK 500 4 ASN A 5 -76.99 -44.68
REMARK 500 4 MET A 6 -49.47 164.48
REMARK 500 4 GLU A 7 151.64 -37.88
REMARK 500 4 ARG A 8 78.53 -101.91
REMARK 500 4 LEU A 12 -51.12 -176.51
REMARK 500 4 GLN A 19 -65.55 -163.94
REMARK 500 4 SER A 20 89.80 66.75
REMARK 500 4 ASP A 25 120.12 169.27
REMARK 500 4 MET A 34 -29.50 -38.13
REMARK 500 4 LYS A 35 157.59 72.04
REMARK 500 4 ALA A 38 -80.90 -85.03
REMARK 500 4 ARG A 44 59.67 31.84
REMARK 500 4 ILE A 45 161.23 -40.55
REMARK 500 4 MET A 50 46.85 81.05
REMARK 500 4 ASN A 55 -141.18 38.10
REMARK 500 4 ASN A 58 89.79 -151.72
REMARK 500 4 GLU A 60 -75.75 -65.18
REMARK 500 4 MET A 62 -83.27 -59.05
REMARK 500 4 SER A 63 49.52 35.43
REMARK 500 4 ASP A 65 29.97 39.77
REMARK 500 4 ASP A 66 28.68 -163.21
REMARK 500 4 ILE A 74 -89.51 -66.71
REMARK 500 4 VAL A 75 -38.79 -37.74
REMARK 500 4 GLN A 77 -129.27 -137.90
REMARK 500 4 THR A 78 160.81 -40.27
REMARK 500 4 SER A 82 46.44 155.20
REMARK 500 4 LEU A 83 145.16 -32.05
REMARK 500 4 ALA A 86 34.09 -91.86
REMARK 500 4 LYS A 87 173.48 52.75
REMARK 500 4 ALA A 88 82.35 179.91
REMARK 500 4 TRP A 89 84.27 81.00
REMARK 500 4 ASP A 90 100.19 -39.64
REMARK 500 4 PRO A 91 -162.75 -75.03
REMARK 500 5 LEU A 4 85.07 -58.71
REMARK 500 5 ASN A 5 175.99 53.44
REMARK 500 5 GLU A 7 169.27 67.05
REMARK 500 5 HIS A 9 87.96 175.78
REMARK 500 5 HIS A 10 143.33 168.65
REMARK 500 5 GLN A 19 -53.72 172.75
REMARK 500 5 SER A 20 -64.09 74.72
REMARK 500 5 ARG A 23 90.60 -64.55
REMARK 500 5 ASP A 25 123.04 163.35
REMARK 500 5 SER A 32 166.81 179.98
REMARK 500 5 MET A 34 -28.57 -38.93
REMARK 500 5 ALA A 38 -86.22 -85.39
REMARK 500 5 ILE A 45 164.85 -42.76
REMARK 500 5 MET A 50 52.36 85.62
REMARK 500 5 ASN A 55 -140.80 37.86
REMARK 500 5 ASN A 58 88.98 -150.09
REMARK 500 5 GLU A 60 -74.55 -62.08
REMARK 500 5 MET A 62 -78.22 -59.13
REMARK 500 5 SER A 63 52.08 31.00
REMARK 500 5 ASP A 65 29.16 41.00
REMARK 500 5 ASP A 66 30.87 -166.79
REMARK 500 5 ILE A 74 -91.40 -66.41
REMARK 500 5 VAL A 75 -38.83 -38.11
REMARK 500 5 GLN A 77 -121.03 -133.11
REMARK 500 5 THR A 78 -178.08 -49.87
REMARK 500 5 SER A 82 45.67 155.60
REMARK 500 5 LEU A 83 143.37 -30.42
REMARK 500 5 ALA A 86 37.69 -93.40
REMARK 500 5 LYS A 87 173.12 53.82
REMARK 500 5 ALA A 88 83.57 172.07
REMARK 500 5 TRP A 89 63.81 75.65
REMARK 500 5 ASP A 90 104.68 -40.81
REMARK 500 5 PRO A 91 -163.37 -75.00
REMARK 500 5 THR A 92 139.73 -39.47
REMARK 500 5 ARG A 94 51.69 36.41
REMARK 500 6 HIS A 9 176.85 54.94
REMARK 500 6 HIS A 10 -62.92 -133.92
REMARK 500 6 PHE A 11 131.81 -37.86
REMARK 500 6 LEU A 12 113.58 176.39
REMARK 500 6 GLN A 19 -57.67 -156.70
REMARK 500 6 SER A 20 70.83 45.18
REMARK 500 6 ARG A 23 106.69 -39.79
REMARK 500 6 ASP A 25 92.30 52.77
REMARK 500 6 MET A 34 31.18 -84.92
REMARK 500 6 LYS A 35 44.34 36.12
REMARK 500 6 ALA A 38 -77.01 -92.95
REMARK 500 6 ARG A 44 54.65 -113.55
REMARK 500 6 ILE A 45 163.24 -41.35
REMARK 500 6 MET A 50 50.71 77.24
REMARK 500 6 ASN A 55 -142.03 38.29
REMARK 500 6 GLU A 60 -74.97 -64.93
REMARK 500 6 MET A 62 -84.71 -60.85
REMARK 500 6 SER A 63 57.29 34.98
REMARK 500 6 ASP A 66 29.44 -162.33
REMARK 500 6 ILE A 74 -92.76 -66.58
REMARK 500 6 VAL A 75 -38.16 -37.85
REMARK 500 6 GLN A 77 -121.96 -130.42
REMARK 500 6 THR A 78 -174.69 -51.43
REMARK 500 6 SER A 82 49.31 158.54
REMARK 500 6 LEU A 83 145.01 -32.34
REMARK 500 6 ALA A 86 41.06 -101.66
REMARK 500 6 LYS A 87 172.24 53.22
REMARK 500 6 ALA A 88 57.22 -179.61
REMARK 500 6 TRP A 89 -175.84 -177.16
REMARK 500 6 PRO A 91 -165.32 -75.07
REMARK 500 7 ASN A 5 179.48 -58.22
REMARK 500 7 MET A 6 118.35 73.48
REMARK 500 7 GLU A 7 109.26 71.35
REMARK 500 7 HIS A 9 103.59 175.03
REMARK 500 7 PHE A 11 179.86 -55.60
REMARK 500 7 LEU A 12 -64.42 -158.45
REMARK 500 7 GLN A 19 -61.37 179.04
REMARK 500 7 SER A 20 167.36 60.17
REMARK 500 7 ASN A 21 -56.21 79.54
REMARK 500 7 ASP A 22 107.46 -40.92
REMARK 500 7 ASP A 25 92.61 171.29
REMARK 500 7 MET A 34 85.54 -62.17
REMARK 500 7 LYS A 35 107.62 -38.94
REMARK 500 7 ALA A 38 -86.91 -76.37
REMARK 500 7 ARG A 44 55.51 -117.24
REMARK 500 7 ILE A 45 164.06 -42.13
REMARK 500 7 MET A 50 48.86 81.28
REMARK 500 7 ASN A 55 -139.42 39.23
REMARK 500 7 PHE A 59 49.85 -79.60
REMARK 500 7 MET A 62 -76.22 -58.59
REMARK 500 7 SER A 63 51.23 30.63
REMARK 500 7 ASP A 65 27.59 42.90
REMARK 500 7 ASP A 66 29.80 -164.92
REMARK 500 7 ILE A 74 -89.67 -64.78
REMARK 500 7 VAL A 75 -37.71 -36.90
REMARK 500 7 GLN A 77 -131.30 -135.06
REMARK 500 7 THR A 78 160.21 -40.04
REMARK 500 7 SER A 82 47.32 156.79
REMARK 500 7 LEU A 83 139.72 -28.43
REMARK 500 7 ALA A 86 39.81 -93.93
REMARK 500 7 LYS A 87 171.12 55.03
REMARK 500 7 ALA A 88 62.66 178.43
REMARK 500 7 TRP A 89 71.24 86.93
REMARK 500 8 ASN A 5 176.62 -51.43
REMARK 500 8 GLU A 7 131.63 -179.94
REMARK 500 8 HIS A 9 76.43 -163.44
REMARK 500 8 LEU A 12 102.25 -176.63
REMARK 500 8 GLN A 19 58.13 175.80
REMARK 500 8 SER A 20 124.92 -39.37
REMARK 500 8 ASP A 22 167.86 63.35
REMARK 500 8 ASP A 25 125.67 161.19
REMARK 500 8 SER A 32 164.27 179.92
REMARK 500 8 MET A 34 92.08 -45.81
REMARK 500 8 LYS A 35 160.08 -41.14
REMARK 500 8 ALA A 38 -75.30 -91.54
REMARK 500 8 ILE A 45 165.29 -47.82
REMARK 500 8 MET A 50 54.48 81.59
REMARK 500 8 ASN A 55 -138.79 38.68
REMARK 500 8 MET A 62 -84.47 -77.34
REMARK 500 8 SER A 63 40.66 31.47
REMARK 500 8 ASP A 65 -32.85 170.45
REMARK 500 8 ILE A 74 -92.55 -63.80
REMARK 500 8 VAL A 75 -38.09 -39.98
REMARK 500 8 GLN A 77 -118.89 -127.78
REMARK 500 8 THR A 78 -173.22 -52.19
REMARK 500 8 SER A 82 47.41 157.98
REMARK 500 8 LEU A 83 141.97 -33.94
REMARK 500 8 ALA A 86 38.34 -92.33
REMARK 500 8 LYS A 87 172.55 53.11
REMARK 500 8 ALA A 88 100.36 -174.14
REMARK 500 8 TRP A 89 -72.65 -42.77
REMARK 500 8 ASP A 90 87.75 43.05
REMARK 500 9 LEU A 4 179.28 -56.51
REMARK 500 9 MET A 6 120.14 -36.16
REMARK 500 9 GLU A 7 76.33 176.41
REMARK 500 9 ARG A 8 99.27 -63.66
REMARK 500 9 HIS A 9 -81.93 -116.89
REMARK 500 9 HIS A 10 -62.70 171.88
REMARK 500 9 PHE A 11 135.26 -37.05
REMARK 500 9 LEU A 12 -66.20 -165.45
REMARK 500 9 GLN A 19 105.21 68.27
REMARK 500 9 ASP A 25 91.59 43.43
REMARK 500 9 SER A 32 169.11 179.97
REMARK 500 9 LYS A 35 -36.34 88.82
REMARK 500 9 ALA A 38 -82.01 -83.04
REMARK 500 9 ILE A 45 164.58 -43.06
REMARK 500 9 MET A 50 49.28 82.89
REMARK 500 9 ASN A 55 -139.68 42.64
REMARK 500 9 MET A 62 -73.78 -86.11
REMARK 500 9 SER A 63 46.95 28.33
REMARK 500 9 ASP A 65 30.48 39.88
REMARK 500 9 ASP A 66 30.14 -163.92
REMARK 500 9 ILE A 74 -92.88 -66.92
REMARK 500 9 VAL A 75 -38.75 -37.88
REMARK 500 9 GLN A 77 -118.11 -135.73
REMARK 500 9 THR A 78 -174.95 -51.60
REMARK 500 9 SER A 82 52.29 157.05
REMARK 500 9 LEU A 83 145.12 -35.71
REMARK 500 9 ALA A 86 37.41 -93.17
REMARK 500 9 LYS A 87 176.16 50.28
REMARK 500 9 ALA A 88 73.29 178.96
REMARK 500 9 TRP A 89 66.87 85.28
REMARK 500 9 ASP A 90 102.29 -43.00
REMARK 500 9 THR A 92 97.70 -36.82
REMARK 500 10 ASN A 5 171.31 53.88
REMARK 500 10 LEU A 12 -65.21 -178.07
REMARK 500 10 GLN A 19 -63.80 -157.56
REMARK 500 10 SER A 20 172.98 59.86
REMARK 500 10 ASN A 21 -54.34 -122.82
REMARK 500 10 ASP A 22 -52.96 -142.14
REMARK 500 10 ASP A 25 105.80 60.25
REMARK 500 10 LYS A 35 41.08 73.59
REMARK 500 10 ALA A 38 -80.50 -84.87
REMARK 500 10 ARG A 44 60.98 38.84
REMARK 500 10 ILE A 45 164.91 -43.53
REMARK 500 10 MET A 50 52.68 81.59
REMARK 500 10 ASN A 55 -140.96 42.08
REMARK 500 10 MET A 62 -73.12 -67.07
REMARK 500 10 SER A 63 51.65 24.55
REMARK 500 10 ASP A 65 29.38 40.53
REMARK 500 10 ASP A 66 32.61 -170.57
REMARK 500 10 ILE A 74 -93.75 -65.03
REMARK 500 10 GLN A 77 -118.32 -132.30
REMARK 500 10 THR A 78 -173.05 -52.12
REMARK 500 10 SER A 82 45.08 153.82
REMARK 500 10 LEU A 83 144.79 -31.36
REMARK 500 10 ALA A 86 41.68 -92.09
REMARK 500 10 LYS A 87 176.80 51.09
REMARK 500 10 ALA A 88 119.94 -179.07
REMARK 500 11 MET A 6 149.76 -175.26
REMARK 500 11 GLN A 19 -47.92 178.99
REMARK 500 11 SER A 20 114.94 64.74
REMARK 500 11 ASP A 22 89.12 -64.01
REMARK 500 11 ARG A 23 66.54 179.40
REMARK 500 11 ASP A 25 124.57 164.23
REMARK 500 11 SER A 32 158.35 179.96
REMARK 500 11 LYS A 35 -106.67 68.08
REMARK 500 11 ALA A 38 -75.09 -83.21
REMARK 500 11 ARG A 44 59.47 38.07
REMARK 500 11 ILE A 45 165.27 -43.61
REMARK 500 11 MET A 50 48.24 84.57
REMARK 500 11 ASN A 55 -140.54 33.92
REMARK 500 11 MET A 62 -85.11 -64.04
REMARK 500 11 SER A 63 49.52 35.83
REMARK 500 11 ASP A 66 43.39 156.21
REMARK 500 11 ILE A 74 -92.14 -66.46
REMARK 500 11 VAL A 75 -37.97 -37.64
REMARK 500 11 GLN A 77 -131.61 -131.04
REMARK 500 11 THR A 78 158.77 -41.70
REMARK 500 11 SER A 82 50.80 156.40
REMARK 500 11 LEU A 83 145.80 -37.13
REMARK 500 11 ALA A 86 40.80 -90.31
REMARK 500 11 LYS A 87 173.76 52.85
REMARK 500 11 ALA A 88 64.54 175.98
REMARK 500 11 TRP A 89 -67.92 85.39
REMARK 500 11 PRO A 91 -164.18 -75.00
REMARK 500 12 LEU A 4 -179.93 -52.26
REMARK 500 12 MET A 6 176.01 69.74
REMARK 500 12 HIS A 9 53.51 -179.61
REMARK 500 12 GLN A 19 -52.57 -160.52
REMARK 500 12 SER A 20 -71.94 66.78
REMARK 500 12 ASN A 21 169.55 72.19
REMARK 500 12 ASP A 25 90.75 49.80
REMARK 500 12 ALA A 38 -81.67 -81.03
REMARK 500 12 ILE A 45 163.39 -47.26
REMARK 500 12 MET A 50 45.12 83.37
REMARK 500 12 ASN A 55 -142.28 39.84
REMARK 500 12 MET A 62 -74.62 -67.46
REMARK 500 12 SER A 63 53.39 27.79
REMARK 500 12 ASP A 65 28.12 42.44
REMARK 500 12 ASP A 66 31.11 -167.44
REMARK 500 12 ILE A 74 -93.31 -67.13
REMARK 500 12 VAL A 75 -38.36 -38.25
REMARK 500 12 GLN A 77 -119.63 -134.43
REMARK 500 12 THR A 78 -175.19 -51.37
REMARK 500 12 SER A 82 46.30 156.45
REMARK 500 12 LEU A 83 147.38 -32.79
REMARK 500 12 LYS A 87 170.88 59.49
REMARK 500 12 ALA A 88 77.52 -160.77
REMARK 500 12 TRP A 89 -64.83 71.23
REMARK 500 12 ASP A 90 89.12 40.48
REMARK 500 13 HIS A 9 -78.71 -123.60
REMARK 500 13 HIS A 10 -172.45 -177.63
REMARK 500 13 PHE A 11 153.15 58.47
REMARK 500 13 LEU A 12 -73.11 -174.22
REMARK 500 13 GLN A 19 72.17 173.00
REMARK 500 13 ASN A 21 -47.65 -176.39
REMARK 500 13 ARG A 23 100.89 175.19
REMARK 500 13 ASP A 25 89.50 43.39
REMARK 500 13 ALA A 38 -94.33 -61.88
REMARK 500 13 ARG A 44 55.79 36.49
REMARK 500 13 ILE A 45 164.57 -42.83
REMARK 500 13 MET A 50 53.09 81.59
REMARK 500 13 ASN A 55 -140.49 36.08
REMARK 500 13 ASN A 58 89.85 -152.10
REMARK 500 13 MET A 62 -81.45 -67.93
REMARK 500 13 SER A 63 49.33 34.15
REMARK 500 13 ASP A 65 29.86 39.68
REMARK 500 13 ASP A 66 28.80 -162.92
REMARK 500 13 ILE A 74 -90.51 -68.15
REMARK 500 13 VAL A 75 -39.30 -38.11
REMARK 500 13 GLN A 77 -129.58 -132.81
REMARK 500 13 THR A 78 166.11 -43.00
REMARK 500 13 SER A 82 49.69 152.50
REMARK 500 13 LEU A 83 152.52 -35.69
REMARK 500 13 LYS A 87 170.94 53.83
REMARK 500 13 ALA A 88 66.76 174.96
REMARK 500 13 ASP A 90 103.41 -176.80
REMARK 500 14 MET A 6 77.91 -151.84
REMARK 500 14 GLU A 7 169.06 58.22
REMARK 500 14 HIS A 10 62.97 -108.33
REMARK 500 14 LEU A 12 -60.95 -169.36
REMARK 500 14 GLN A 19 67.32 173.45
REMARK 500 14 SER A 20 160.49 -49.01
REMARK 500 14 ASN A 21 85.99 65.27
REMARK 500 14 ASP A 22 -52.34 -149.74
REMARK 500 14 ARG A 23 69.29 177.74
REMARK 500 14 ASP A 25 118.72 168.99
REMARK 500 14 SER A 32 157.11 179.84
REMARK 500 14 MET A 34 88.98 -51.89
REMARK 500 14 LYS A 35 159.68 -40.18
REMARK 500 14 ALA A 38 -76.71 -85.63
REMARK 500 14 ARG A 44 58.14 34.92
REMARK 500 14 ILE A 45 163.76 -42.11
REMARK 500 14 MET A 50 48.29 83.37
REMARK 500 14 ASN A 55 -139.41 35.49
REMARK 500 14 ASN A 58 89.88 -151.34
REMARK 500 14 MET A 62 -83.88 -73.10
REMARK 500 14 SER A 63 42.67 30.22
REMARK 500 14 ASP A 65 -37.49 166.09
REMARK 500 14 ILE A 74 -92.40 -63.34
REMARK 500 14 VAL A 75 -39.66 -39.76
REMARK 500 14 GLN A 77 -116.52 -126.37
REMARK 500 14 THR A 78 -174.79 -50.62
REMARK 500 14 SER A 82 44.01 155.69
REMARK 500 14 LEU A 83 146.96 -37.37
REMARK 500 14 ALA A 86 41.79 -94.83
REMARK 500 14 LYS A 87 174.71 52.16
REMARK 500 14 ALA A 88 82.24 -177.88
REMARK 500 14 TRP A 89 148.31 87.31
REMARK 500 14 PRO A 91 -163.31 -75.05
REMARK 500 15 MET A 6 124.99 -179.60
REMARK 500 15 ARG A 8 85.47 40.31
REMARK 500 15 PHE A 11 113.77 58.72
REMARK 500 15 LEU A 12 -62.31 -142.99
REMARK 500 15 GLN A 19 -47.45 173.96
REMARK 500 15 SER A 20 148.69 60.66
REMARK 500 15 ASP A 22 -82.09 63.56
REMARK 500 15 ASP A 25 105.72 169.39
REMARK 500 15 SER A 32 170.77 179.92
REMARK 500 15 LYS A 35 40.68 37.58
REMARK 500 15 ALA A 38 -83.66 -87.85
REMARK 500 15 ARG A 44 54.05 -113.84
REMARK 500 15 ILE A 45 164.26 -42.18
REMARK 500 15 MET A 50 50.32 82.33
REMARK 500 15 ASN A 55 -140.64 38.89
REMARK 500 15 PHE A 59 46.61 -78.15
REMARK 500 15 GLU A 60 -77.55 -57.13
REMARK 500 15 MET A 62 -83.44 -46.90
REMARK 500 15 SER A 63 23.12 45.47
REMARK 500 15 ASP A 65 44.92 -83.80
REMARK 500 15 ASP A 66 43.56 160.45
REMARK 500 15 ILE A 74 -91.96 -69.27
REMARK 500 15 VAL A 75 -37.83 -37.86
REMARK 500 15 GLN A 77 -123.80 -137.02
REMARK 500 15 THR A 78 174.19 -46.52
REMARK 500 15 SER A 82 45.34 151.82
REMARK 500 15 LEU A 83 144.35 -31.72
REMARK 500 15 ALA A 86 39.39 -92.78
REMARK 500 15 LYS A 87 173.60 53.14
REMARK 500 15 ALA A 88 90.20 176.97
REMARK 500 15 TRP A 89 117.73 74.16
REMARK 500 15 ASP A 90 99.95 -39.37
REMARK 500 15 PRO A 91 -162.84 -74.97
REMARK 500 16 MET A 6 123.67 169.59
REMARK 500 16 GLU A 7 169.29 -44.63
REMARK 500 16 HIS A 9 166.20 169.07
REMARK 500 16 HIS A 10 92.19 -57.31
REMARK 500 16 LEU A 12 96.54 72.13
REMARK 500 16 GLN A 19 86.66 173.72
REMARK 500 16 ASP A 22 87.52 177.65
REMARK 500 16 ARG A 23 123.76 66.67
REMARK 500 16 ASP A 25 88.29 41.63
REMARK 500 16 ALA A 38 -85.14 -63.46
REMARK 500 16 ARG A 44 55.13 30.80
REMARK 500 16 ILE A 45 164.20 -42.26
REMARK 500 16 MET A 50 48.44 84.73
REMARK 500 16 ASN A 55 -140.77 37.77
REMARK 500 16 ASN A 58 89.38 -151.66
REMARK 500 16 PHE A 59 48.46 -80.20
REMARK 500 16 MET A 62 -81.88 -57.83
REMARK 500 16 SER A 63 53.85 32.58
REMARK 500 16 ASP A 65 26.08 45.52
REMARK 500 16 ASP A 66 29.45 -164.31
REMARK 500 16 ILE A 74 -91.23 -67.89
REMARK 500 16 VAL A 75 -37.64 -36.65
REMARK 500 16 GLN A 77 -130.53 -133.39
REMARK 500 16 THR A 78 167.44 -43.21
REMARK 500 16 SER A 82 47.36 154.14
REMARK 500 16 LEU A 83 142.54 -30.09
REMARK 500 16 ALA A 86 36.74 -93.46
REMARK 500 16 LYS A 87 171.46 54.22
REMARK 500 16 ALA A 88 60.76 -179.72
REMARK 500 16 TRP A 89 66.37 86.79
REMARK 500 16 THR A 92 89.65 40.59
REMARK 500 17 LEU A 4 173.74 -48.93
REMARK 500 17 ASN A 5 -169.06 -114.80
REMARK 500 17 MET A 6 169.52 58.20
REMARK 500 17 PHE A 11 111.40 -171.62
REMARK 500 17 LEU A 12 120.94 179.81
REMARK 500 17 GLN A 19 -45.51 -162.39
REMARK 500 17 SER A 20 92.49 61.96
REMARK 500 17 ASN A 21 155.74 178.12
REMARK 500 17 ASP A 22 101.86 -42.04
REMARK 500 17 ARG A 23 90.63 -177.19
REMARK 500 17 ASP A 25 88.28 40.35
REMARK 500 17 SER A 32 160.94 180.00
REMARK 500 17 MET A 34 93.01 -52.80
REMARK 500 17 LYS A 35 169.07 -45.01
REMARK 500 17 ALA A 38 -81.78 -85.73
REMARK 500 17 ARG A 44 55.04 -119.02
REMARK 500 17 ILE A 45 162.32 -41.35
REMARK 500 17 MET A 50 48.60 81.32
REMARK 500 17 ASN A 55 -137.84 34.70
REMARK 500 17 ASN A 61 -50.79 77.89
REMARK 500 17 SER A 63 54.50 27.25
REMARK 500 17 ASP A 66 29.33 -164.10
REMARK 500 17 ILE A 74 -92.70 -65.76
REMARK 500 17 VAL A 75 -38.44 -38.54
REMARK 500 17 GLN A 77 -116.74 -130.71
REMARK 500 17 THR A 78 -172.30 -52.58
REMARK 500 17 SER A 82 45.90 155.32
REMARK 500 17 LEU A 83 145.98 -33.44
REMARK 500 17 ALA A 86 41.07 -94.01
REMARK 500 17 LYS A 87 175.02 51.63
REMARK 500 17 ALA A 88 80.83 -173.54
REMARK 500 17 TRP A 89 93.87 77.26
REMARK 500 17 PRO A 91 -164.46 -75.05
REMARK 500 17 ARG A 94 58.18 39.45
REMARK 500 18 ASN A 5 175.59 52.67
REMARK 500 18 MET A 6 77.98 -112.02
REMARK 500 18 ARG A 8 84.98 -178.86
REMARK 500 18 HIS A 9 72.66 179.02
REMARK 500 18 HIS A 10 144.50 173.29
REMARK 500 18 GLN A 19 -57.64 175.04
REMARK 500 18 SER A 20 148.85 63.84
REMARK 500 18 ASP A 22 89.81 46.67
REMARK 500 18 ARG A 23 95.62 54.06
REMARK 500 18 ASP A 25 98.79 65.95
REMARK 500 18 MET A 34 85.09 -63.74
REMARK 500 18 LYS A 35 129.48 -38.99
REMARK 500 18 ALA A 38 -74.57 -84.04
REMARK 500 18 ARG A 44 44.96 -95.88
REMARK 500 18 ILE A 45 160.72 -40.57
REMARK 500 18 MET A 50 54.63 85.71
REMARK 500 18 ASN A 55 -141.32 39.17
REMARK 500 18 MET A 62 -76.59 -70.62
REMARK 500 18 SER A 63 52.65 26.46
REMARK 500 18 ASP A 65 28.93 41.09
REMARK 500 18 ASP A 66 30.64 -166.95
REMARK 500 18 ILE A 74 -92.68 -67.59
REMARK 500 18 VAL A 75 -38.14 -38.29
REMARK 500 18 GLN A 77 -118.76 -133.67
REMARK 500 18 THR A 78 -173.44 -52.06
REMARK 500 18 SER A 82 46.15 153.36
REMARK 500 18 LEU A 83 144.11 -31.12
REMARK 500 18 ALA A 86 37.94 -92.96
REMARK 500 18 LYS A 87 172.50 53.54
REMARK 500 18 ALA A 88 91.61 -177.60
REMARK 500 18 TRP A 89 64.91 67.42
REMARK 500 18 THR A 92 125.94 -38.72
REMARK 500 19 GLU A 7 156.43 177.71
REMARK 500 19 ARG A 8 73.50 -172.92
REMARK 500 19 HIS A 9 -63.12 -177.26
REMARK 500 19 HIS A 10 156.78 -39.48
REMARK 500 19 LEU A 12 -64.89 -130.26
REMARK 500 19 GLN A 19 40.74 -143.30
REMARK 500 19 SER A 20 121.88 -38.94
REMARK 500 19 ASN A 21 124.11 177.75
REMARK 500 19 ASP A 22 169.63 66.39
REMARK 500 19 ASP A 25 116.74 61.06
REMARK 500 19 SER A 32 161.11 179.96
REMARK 500 19 MET A 34 89.17 -63.94
REMARK 500 19 LYS A 35 103.41 -39.70
REMARK 500 19 ALA A 38 -87.63 -77.70
REMARK 500 19 ARG A 44 76.12 -160.11
REMARK 500 19 ILE A 45 179.68 -50.09
REMARK 500 19 MET A 50 47.84 84.79
REMARK 500 19 ASN A 55 -139.27 36.14
REMARK 500 19 PHE A 59 38.51 -81.46
REMARK 500 19 ASN A 61 -44.58 81.27
REMARK 500 19 SER A 63 58.91 22.66
REMARK 500 19 ASP A 65 24.23 48.32
REMARK 500 19 ASP A 66 34.14 -173.00
REMARK 500 19 ILE A 74 -92.32 -66.51
REMARK 500 19 VAL A 75 -38.28 -38.44
REMARK 500 19 GLN A 77 -119.06 -132.14
REMARK 500 19 THR A 78 -173.57 -52.09
REMARK 500 19 SER A 82 50.03 155.91
REMARK 500 19 LEU A 83 141.80 -32.61
REMARK 500 19 LYS A 87 171.02 57.94
REMARK 500 19 ALA A 88 58.31 175.30
REMARK 500 19 PRO A 91 -162.60 -74.95
REMARK 500 20 LEU A 4 175.20 -50.89
REMARK 500 20 ASN A 5 -70.24 -85.11
REMARK 500 20 MET A 6 -149.09 -166.03
REMARK 500 20 GLU A 7 119.01 71.86
REMARK 500 20 HIS A 9 128.80 174.49
REMARK 500 20 HIS A 10 173.67 54.64
REMARK 500 20 PHE A 11 177.32 59.22
REMARK 500 20 LEU A 12 90.33 81.39
REMARK 500 20 GLN A 19 -47.80 -174.60
REMARK 500 20 SER A 20 167.19 60.88
REMARK 500 20 ASN A 21 97.44 62.38
REMARK 500 20 ASP A 25 100.44 171.20
REMARK 500 20 MET A 34 79.99 -69.30
REMARK 500 20 LYS A 35 128.64 -39.09
REMARK 500 20 ALA A 38 -83.18 -80.20
REMARK 500 20 ARG A 44 51.03 -112.58
REMARK 500 20 ILE A 45 164.94 -42.36
REMARK 500 20 MET A 50 52.42 84.03
REMARK 500 20 ASN A 55 -138.92 34.40
REMARK 500 20 ASN A 61 -46.13 80.34
REMARK 500 20 SER A 63 47.97 27.05
REMARK 500 20 ASP A 66 24.46 -156.74
REMARK 500 20 ILE A 74 -91.91 -65.59
REMARK 500 20 VAL A 75 -38.99 -39.09
REMARK 500 20 GLN A 77 -118.89 -130.45
REMARK 500 20 THR A 78 -177.32 -50.11
REMARK 500 20 SER A 82 49.43 160.22
REMARK 500 20 LEU A 83 140.29 -31.09
REMARK 500 20 ALA A 86 40.82 -93.79
REMARK 500 20 LYS A 87 171.85 53.77
REMARK 500 20 ALA A 88 62.00 176.67
REMARK 500 20 TRP A 89 -171.33 177.68
REMARK 500
REMARK 500 REMARK: NULL
DBREF 1MC7 A 1 95 UNP P51141 DVL1_MOUSE 251 345
SEQADV 1MC7 ALA A 88 UNP P51141 CYS 338 ENGINEERED
SEQRES 1 A 95 THR VAL THR LEU ASN MET GLU ARG HIS HIS PHE LEU GLY
SEQRES 2 A 95 ILE SER ILE VAL GLY GLN SER ASN ASP ARG GLY ASP GLY
SEQRES 3 A 95 GLY ILE TYR ILE GLY SER ILE MET LYS GLY GLY ALA VAL
SEQRES 4 A 95 ALA ALA ASP GLY ARG ILE GLU PRO GLY ASP MET LEU LEU
SEQRES 5 A 95 GLN VAL ASN ASP VAL ASN PHE GLU ASN MET SER ASN ASP
SEQRES 6 A 95 ASP ALA VAL ARG VAL LEU ARG GLU ILE VAL SER GLN THR
SEQRES 7 A 95 GLY PRO ILE SER LEU THR VAL ALA LYS ALA TRP ASP PRO
SEQRES 8 A 95 THR PRO ARG SER
HELIX 1 1 ALA A 38 GLY A 43 1 6
HELIX 2 2 GLU A 46 ASP A 49 5 4
HELIX 3 3 ALA A 67 GLN A 77 1 11
SHEET 1 A 2 ILE A 14 VAL A 17 0
SHEET 2 A 2 TYR A 29 ILE A 33 -1 O GLY A 31 N SER A 15
SHEET 1 B 2 LEU A 51 VAL A 54 0
SHEET 2 B 2 LEU A 83 VAL A 85 -1 O THR A 84 N GLN A 53
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
