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Database: PDB
Entry: 1MED
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Original site: 1MED 
HEADER    AMINOACYL-TRNA SYNTHASE                 09-NOV-92   1MED              
TITLE     METHIONYL-TRNA SYNTHETASE ZINC BINDING DOMAIN. 3D STRUCTURE AND       
TITLE    2 HOMOLOGY WITH RUBREDOXIN AND GAG RETROVIRAL PROTEINS                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: METHIONYL-TRNA SYNTHETASE;                                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 EC: 6.1.1.10;                                                        
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   3 ORGANISM_TAXID: 562;                                                 
SOURCE   4 GENE: GAG;                                                           
SOURCE   5 EXPRESSION_SYSTEM_GENE: GAG                                          
KEYWDS    AMINOACYL-TRNA SYNTHASE                                               
EXPDTA    SOLUTION NMR                                                          
NUMMDL    11                                                                    
AUTHOR    D.FOURMY,F.DARDEL                                                     
REVDAT   6   29-NOV-17 1MED    1       REMARK HELIX                             
REVDAT   5   24-FEB-09 1MED    1       VERSN                                    
REVDAT   4   01-APR-03 1MED    1       JRNL                                     
REVDAT   3   15-JAN-95 1MED    1       COMPND                                   
REVDAT   2   31-JAN-94 1MED    3       FTNOTE ATOM                              
REVDAT   1   31-OCT-93 1MED    0                                                
JRNL        AUTH   D.FOURMY,F.DARDEL,S.BLANQUET                                 
JRNL        TITL   METHIONYL-TRNA SYNTHETASE ZINC BINDING DOMAIN.               
JRNL        TITL 2 THREE-DIMENSIONAL STRUCTURE AND HOMOLOGY WITH RUBREDOXIN AND 
JRNL        TITL 3 GAG RETROVIRAL PROTEINS.                                     
JRNL        REF    J.MOL.BIOL.                   V. 231  1078 1993              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   8515466                                                      
JRNL        DOI    10.1006/JMBI.1993.1353                                       
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   D.FOURMY,T.MEINNEL,Y.MECHULAM,S.BLANQUET                     
REMARK   1  TITL   MAPPING OF THE ZINC BINDING DOMAIN OF ESCHERICHIA COLI       
REMARK   1  TITL 2 METHIONYL-TRNA SYNTHETASE                                    
REMARK   1  REF    J.MOL.BIOL.                   V. 231  1068 1993              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   S.BRUNIE,C.ZELWER,J.-L.RISLER                                
REMARK   1  TITL   CRYSTALLOGRAPHIC STUDY AT 2.5 ANGSTROMS RESOLUTION OF THE    
REMARK   1  TITL 2 INTERACTION OF METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA    
REMARK   1  TITL 3 COLI WITH ATP                                                
REMARK   1  REF    J.MOL.BIOL.                   V. 216   411 1990              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR                                               
REMARK   3   AUTHORS     : NULL                                                 
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1MED COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 100 THE DEPOSITION ID IS D_1000174971.                                   
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : NULL                               
REMARK 210  PH                             : NULL                               
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : NULL                               
REMARK 210  SPECTROMETER MODEL             : NULL                               
REMARK 210  SPECTROMETER MANUFACTURER      : NULL                               
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NULL                               
REMARK 210   METHOD USED                   : NULL                               
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 11                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A                                     
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =   8.7 DEGREES          
REMARK 500  1 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. = -10.5 DEGREES          
REMARK 500  1 PHE A   5   N   -  CA  -  C   ANGL. DEV. = -17.0 DEGREES          
REMARK 500  1 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -12.1 DEGREES          
REMARK 500  2 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =   5.8 DEGREES          
REMARK 500  2 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =  -8.1 DEGREES          
REMARK 500  2 VAL A   6   CA  -  C   -  N   ANGL. DEV. = -14.7 DEGREES          
REMARK 500  2 LYS A   7   N   -  CA  -  C   ANGL. DEV. = -20.4 DEGREES          
REMARK 500  2 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -10.9 DEGREES          
REMARK 500  2 ALA A  28   N   -  CA  -  C   ANGL. DEV. =  21.4 DEGREES          
REMARK 500  3 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. = -10.1 DEGREES          
REMARK 500  3 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   8.8 DEGREES          
REMARK 500  3 CYS A  10   CA  -  CB  -  SG  ANGL. DEV. =  12.7 DEGREES          
REMARK 500  3 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -12.6 DEGREES          
REMARK 500  3 CYS A  23   CA  -  CB  -  SG  ANGL. DEV. =  10.2 DEGREES          
REMARK 500  4 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. = -12.3 DEGREES          
REMARK 500  4 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =  11.6 DEGREES          
REMARK 500  4 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -14.1 DEGREES          
REMARK 500  4 CYS A  26   CA  -  CB  -  SG  ANGL. DEV. =   8.5 DEGREES          
REMARK 500  4 ALA A  28   N   -  CA  -  C   ANGL. DEV. =  21.1 DEGREES          
REMARK 500  5 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =  -9.8 DEGREES          
REMARK 500  5 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   7.7 DEGREES          
REMARK 500  5 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -11.4 DEGREES          
REMARK 500  6 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. = -10.8 DEGREES          
REMARK 500  6 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   8.0 DEGREES          
REMARK 500  6 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -14.6 DEGREES          
REMARK 500  7 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =  -7.8 DEGREES          
REMARK 500  7 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   5.2 DEGREES          
REMARK 500  7 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -13.9 DEGREES          
REMARK 500  8 VAL A   6   CA  -  CB  -  CG1 ANGL. DEV. =  -9.2 DEGREES          
REMARK 500  8 VAL A   6   CA  -  C   -  N   ANGL. DEV. = -15.5 DEGREES          
REMARK 500  8 LYS A   7   N   -  CA  -  C   ANGL. DEV. = -16.2 DEGREES          
REMARK 500  8 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -13.0 DEGREES          
REMARK 500  8 CYS A  26   CA  -  CB  -  SG  ANGL. DEV. =   7.8 DEGREES          
REMARK 500  9 PHE A   5   CA  -  CB  -  CG  ANGL. DEV. = -17.2 DEGREES          
REMARK 500  9 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =  -9.8 DEGREES          
REMARK 500  9 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   7.3 DEGREES          
REMARK 500  9 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -13.5 DEGREES          
REMARK 500  9 CYS A  26   CA  -  CB  -  SG  ANGL. DEV. =   6.9 DEGREES          
REMARK 500 10 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =  -9.4 DEGREES          
REMARK 500 10 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   6.8 DEGREES          
REMARK 500 10 CYS A  13   CA  -  CB  -  SG  ANGL. DEV. =   7.2 DEGREES          
REMARK 500 10 LYS A  14   N   -  CA  -  CB  ANGL. DEV. = -12.1 DEGREES          
REMARK 500 11 PHE A   5   CB  -  CG  -  CD2 ANGL. DEV. =  -9.4 DEGREES          
REMARK 500 11 PHE A   5   CB  -  CG  -  CD1 ANGL. DEV. =   6.8 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ARG A   4      -64.13    -21.08                                   
REMARK 500  1 LYS A   7       64.09      3.79                                   
REMARK 500  1 LYS A  14       76.09     49.82                                   
REMARK 500  1 PRO A  16      -41.28    -21.96                                   
REMARK 500  1 GLN A  18       73.77   -100.63                                   
REMARK 500  1 ASN A  22       99.06     -8.99                                   
REMARK 500  1 CYS A  23      107.71    -26.18                                   
REMARK 500  2 SER A   2       68.25   -112.90                                   
REMARK 500  2 ASP A   3       69.51   -114.70                                   
REMARK 500  2 LYS A   7       40.09   -140.26                                   
REMARK 500  2 LYS A  14       76.57     57.30                                   
REMARK 500  2 PRO A  16      -36.57    -27.44                                   
REMARK 500  2 ASN A  22       69.23      1.10                                   
REMARK 500  2 CYS A  23      127.73     -1.84                                   
REMARK 500  2 VAL A  25      -59.38   -124.28                                   
REMARK 500  3 ARG A   4      -58.29     96.00                                   
REMARK 500  3 LYS A   7       62.01     25.45                                   
REMARK 500  3 CYS A  10       91.60    -49.38                                   
REMARK 500  3 LYS A  14       80.34    -44.12                                   
REMARK 500  3 ASP A  17       86.80   -150.13                                   
REMARK 500  3 ASP A  21       39.76    -92.31                                   
REMARK 500  3 ASN A  22       65.74      2.42                                   
REMARK 500  3 CYS A  23       52.24     26.18                                   
REMARK 500  3 CYS A  26      -52.68   -123.77                                   
REMARK 500  4 LYS A   7       71.50     98.03                                   
REMARK 500  4 LYS A  14       77.19      6.52                                   
REMARK 500  4 PRO A  16      -40.48    -15.45                                   
REMARK 500  4 GLN A  18       65.96   -100.94                                   
REMARK 500  4 ASN A  22      124.64     64.94                                   
REMARK 500  4 CYS A  23      102.26    -18.43                                   
REMARK 500  5 ARG A   4      -63.22     90.79                                   
REMARK 500  5 PHE A   5     -149.15   -132.43                                   
REMARK 500  5 LYS A   7       89.53     85.17                                   
REMARK 500  5 LYS A  14       87.83     52.57                                   
REMARK 500  5 PRO A  16      -56.59    -10.05                                   
REMARK 500  5 GLN A  18       70.72   -102.31                                   
REMARK 500  5 ASN A  22       82.62     -8.69                                   
REMARK 500  5 CYS A  23      100.41     -3.19                                   
REMARK 500  5 GLU A  24       41.06   -101.46                                   
REMARK 500  5 VAL A  25      -58.17   -120.21                                   
REMARK 500  6 ARG A   4      -66.77    -25.96                                   
REMARK 500  6 LYS A   7       86.01    101.54                                   
REMARK 500  6 LYS A  14       75.17     21.73                                   
REMARK 500  6 PRO A  16      -49.54    -14.72                                   
REMARK 500  6 ASN A  22       74.92     12.21                                   
REMARK 500  6 CYS A  23       91.72     16.79                                   
REMARK 500  7 SER A   2      162.93     69.33                                   
REMARK 500  7 ARG A   4      -60.79    -24.16                                   
REMARK 500  7 LYS A   7       72.47     40.49                                   
REMARK 500  7 CYS A  10       96.39    -50.65                                   
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS      87 RAMACHANDRAN OUTLIERS.                        
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 CYS A   10     PRO A   11          1      -138.35                    
REMARK 500 SER A   15     PRO A   16          1      -129.37                    
REMARK 500 CYS A   10     PRO A   11          2      -133.69                    
REMARK 500 SER A   15     PRO A   16          2      -126.85                    
REMARK 500 CYS A   10     PRO A   11          4      -147.34                    
REMARK 500 SER A   15     PRO A   16          4      -136.59                    
REMARK 500 CYS A   10     PRO A   11          5      -132.18                    
REMARK 500 CYS A   10     PRO A   11          6      -143.86                    
REMARK 500 SER A   15     PRO A   16          6      -146.12                    
REMARK 500 CYS A   10     PRO A   11          8      -148.67                    
REMARK 500 SER A   15     PRO A   16          8      -145.34                    
REMARK 500 CYS A   10     PRO A   11          9      -144.95                    
REMARK 500 SER A   15     PRO A   16          9      -125.87                    
REMARK 500 CYS A   10     PRO A   11         10      -138.47                    
REMARK 500 SER A   15     PRO A   16         10      -139.22                    
REMARK 500 CYS A   10     PRO A   11         11      -147.33                    
REMARK 500 SER A   15     PRO A   16         11      -124.00                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A   4         0.27    SIDE CHAIN                              
REMARK 500  2 ARG A   4         0.17    SIDE CHAIN                              
REMARK 500  3 ARG A   4         0.21    SIDE CHAIN                              
REMARK 500  4 ARG A   4         0.19    SIDE CHAIN                              
REMARK 500  5 ARG A   4         0.32    SIDE CHAIN                              
REMARK 500  6 ARG A   4         0.20    SIDE CHAIN                              
REMARK 500  7 ARG A   4         0.29    SIDE CHAIN                              
REMARK 500  8 ARG A   4         0.30    SIDE CHAIN                              
REMARK 500  9 ARG A   4         0.31    SIDE CHAIN                              
REMARK 500 10 ARG A   4         0.31    SIDE CHAIN                              
REMARK 500 11 ARG A   4         0.32    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                             
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                              ZN A  29  ZN                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 CYS A  10   SG                                                     
REMARK 620 2 CYS A  26   SG  109.4                                              
REMARK 620 3 CYS A  23   SG  104.8 110.9                                        
REMARK 620 4 CYS A  13   SG  109.1 113.1 109.3                                  
REMARK 620 N                    1     2     3                                   
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: ZNC                                                 
REMARK 800 EVIDENCE_CODE: UNKNOWN                                               
REMARK 800 SITE_DESCRIPTION: NULL                                               
REMARK 800                                                                      
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN A 29                   
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1MEA   RELATED DB: PDB                                   
DBREF  1MED A    3    28  UNP    P00959   SYM_ECOLI      138    163             
SEQRES   1 A   28  GLY SER ASP ARG PHE VAL LYS GLY THR CYS PRO LYS CYS          
SEQRES   2 A   28  LYS SER PRO ASP GLN TYR GLY ASP ASN CYS GLU VAL CYS          
SEQRES   3 A   28  GLY ALA                                                      
HET     ZN  A  29       1                                                       
HETNAM      ZN ZINC ION                                                         
FORMUL   2   ZN    ZN 2+                                                        
LINK        ZN    ZN A  29                 SG  CYS A  10     1555   1555  2.29  
LINK        ZN    ZN A  29                 SG  CYS A  26     1555   1555  2.30  
LINK        ZN    ZN A  29                 SG  CYS A  23     1555   1555  2.18  
LINK        ZN    ZN A  29                 SG  CYS A  13     1555   1555  2.30  
SITE     1 ZNC  4 CYS A  10  CYS A  13  CYS A  23  CYS A  26                    
SITE     1 AC1  4 CYS A  10  CYS A  13  CYS A  23  CYS A  26                    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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