GenomeNet

Database: PDB
Entry: 1MJ1
LinkDB: 1MJ1
Original site: 1MJ1 
HEADER    RIBOSOME                                26-AUG-02   1MJ1              
TITLE     FITTING THE TERNARY COMPLEX OF EF-TU/TRNA/GTP AND RIBOSOMAL PROTEINS  
TITLE    2 INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME                     
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: PHE-TRNA;                                                  
COMPND   3 CHAIN: D, C;                                                         
COMPND   4 OTHER_DETAILS: TAKEN FROM PDB ENTRIES 1GIX, 1TRA;                    
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: SARCIN-RICIN LOOP OF 23SRRNA;                              
COMPND   7 CHAIN: Q;                                                            
COMPND   8 OTHER_DETAILS: TAKEN FROM PDB ENTRY 1GIY;                            
COMPND   9 MOL_ID: 3;                                                           
COMPND  10 MOLECULE: HELIX 69 OF 23S RRNA;                                      
COMPND  11 CHAIN: R;                                                            
COMPND  12 OTHER_DETAILS: TAKEN FROM PDB ENTRY 1GIY;                            
COMPND  13 MOL_ID: 4;                                                           
COMPND  14 MOLECULE: ELONGATION FACTOR TU;                                      
COMPND  15 CHAIN: A;                                                            
COMPND  16 OTHER_DETAILS: TAKEN FROM PDB ENTRY 1B23;                            
COMPND  17 MOL_ID: 5;                                                           
COMPND  18 MOLECULE: S12 RIBOSOMAL PROTEIN;                                     
COMPND  19 CHAIN: O;                                                            
COMPND  20 OTHER_DETAILS: TAKEN FROM PDB ENTRY 1GIX;                            
COMPND  21 MOL_ID: 6;                                                           
COMPND  22 MOLECULE: S13 RIBOSOMAL PROTEIN;                                     
COMPND  23 CHAIN: P;                                                            
COMPND  24 OTHER_DETAILS: TAKEN FROM PDB ENTRY 1GIX;                            
COMPND  25 MOL_ID: 7;                                                           
COMPND  26 MOLECULE: L11 RIBOSOMAL PROTEIN;                                     
COMPND  27 CHAIN: L;                                                            
COMPND  28 OTHER_DETAILS: TAKEN FROM PDB ENTRY 1GIY                             
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   3 ORGANISM_TAXID: 562;                                                 
SOURCE   4 MOL_ID: 2;                                                           
SOURCE   5 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   6 ORGANISM_TAXID: 562;                                                 
SOURCE   7 MOL_ID: 3;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE   9 ORGANISM_TAXID: 562;                                                 
SOURCE  10 MOL_ID: 4;                                                           
SOURCE  11 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE  12 ORGANISM_TAXID: 562;                                                 
SOURCE  13 MOL_ID: 5;                                                           
SOURCE  14 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE  15 ORGANISM_TAXID: 562;                                                 
SOURCE  16 MOL_ID: 6;                                                           
SOURCE  17 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE  18 ORGANISM_TAXID: 562;                                                 
SOURCE  19 MOL_ID: 7;                                                           
SOURCE  20 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI;                               
SOURCE  21 ORGANISM_TAXID: 562                                                  
KEYWDS    70S RIBOSOME, LOW RESOLUTION MODEL TERNARY COMPLEX, EF-TU, RIBOSOME   
EXPDTA    ELECTRON MICROSCOPY                                                   
MDLTYP    CA ATOMS ONLY, CHAIN O, P, L; P ATOMS ONLY, CHAIN Q, R                
AUTHOR    H.STARK,M.V.RODNINA,H.-J.WIEDEN,F.ZEMLIN,W.WINTERMEYER,M.VANHEEL      
REVDAT   6   18-JUL-18 1MJ1    1       REMARK                                   
REVDAT   5   13-JUL-11 1MJ1    1       VERSN                                    
REVDAT   4   24-FEB-09 1MJ1    1       VERSN                                    
REVDAT   3   23-DEC-03 1MJ1    1       TITLE  MODRES                            
REVDAT   2   08-NOV-02 1MJ1    1       JRNL                                     
REVDAT   1   01-NOV-02 1MJ1    0                                                
JRNL        AUTH   H.STARK,M.V.RODNINA,H.-J.WIEDEN,F.ZEMLIN,W.WINTERMEYER,      
JRNL        AUTH 2 M.VAN HEEL                                                   
JRNL        TITL   RIBOSOME INTERACTIONS OF AMINOACYL-TRNA AND ELONGATION       
JRNL        TITL 2 FACTOR TU IN THE CODON RECOGNITION COMPLEX                   
JRNL        REF    NAT.STRUCT.BIOL.              V.   9   849 2002              
JRNL        REFN                   ISSN 1072-8368                               
JRNL        PMID   12379845                                                     
REMARK   2                                                                      
REMARK   2 RESOLUTION.   13.00 ANGSTROMS.                                       
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   SOFTWARE PACKAGES      : SITUS, IMAGIC, AMIRA                      
REMARK   3   RECONSTRUCTION SCHEMA  : NULL                                      
REMARK   3                                                                      
REMARK   3 EM MAP-MODEL FITTING AND REFINEMENT                                  
REMARK   3   PDB ENTRY                    : 1B23                                
REMARK   3   REFINEMENT SPACE             : REAL                                
REMARK   3   REFINEMENT PROTOCOL          : RIGID BODY FIT                      
REMARK   3   REFINEMENT TARGET            : BEST VISUAL FIT USING THE PROGRAM   
REMARK   3                                  AMIRA (RIBOSOMAL PROTEINS), BEST    
REMARK   3                                  FIT USING THE PROGRAM SITUS (EF-    
REMARK   3                                  TU)                                 
REMARK   3   OVERALL ANISOTROPIC B VALUE  : NULL                                
REMARK   3                                                                      
REMARK   3 FITTING PROCEDURE : REFINEMENT PROTOCOL--RIGID BODY DETAILS--FOR     
REMARK   3  S12, S13, SRL, HELIX69 ANS L11 ONLY BACKBONE COORDINATES ARE        
REMARK   3  DEPOSITED                                                           
REMARK   3                                                                      
REMARK   3 EM IMAGE RECONSTRUCTION STATISTICS                                   
REMARK   3   NOMINAL PIXEL SIZE (ANGSTROMS)    : NULL                           
REMARK   3   ACTUAL PIXEL SIZE  (ANGSTROMS)    : NULL                           
REMARK   3   EFFECTIVE RESOLUTION (ANGSTROMS)  : 13.00                          
REMARK   3   NUMBER OF PARTICLES               : 24000                          
REMARK   3   CTF CORRECTION METHOD             : NULL                           
REMARK   3                                                                      
REMARK   3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL                    
REMARK   3                                                                      
REMARK   3 OTHER DETAILS: NULL                                                  
REMARK   4                                                                      
REMARK   4 1MJ1 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 02-SEP-02.                  
REMARK 100 THE DEPOSITION ID IS D_1000016946.                                   
REMARK 245                                                                      
REMARK 245 EXPERIMENTAL DETAILS                                                 
REMARK 245   RECONSTRUCTION METHOD          : SINGLE PARTICLE                   
REMARK 245   SPECIMEN TYPE                  : VITREOUS ICE (CRYO EM)            
REMARK 245                                                                      
REMARK 245 ELECTRON MICROSCOPE SAMPLE                                           
REMARK 245   SAMPLE TYPE                    : PARTICLE                          
REMARK 245   PARTICLE TYPE                  : POINT                             
REMARK 245   NAME OF SAMPLE                 : EF-TU/TRNA/GTP E. COLI 70S        
REMARK 245                                    RIBOSOME                          
REMARK 245   SAMPLE CONCENTRATION (MG ML-1) : NULL                              
REMARK 245   SAMPLE SUPPORT DETAILS         : NULL                              
REMARK 245   SAMPLE VITRIFICATION DETAILS   : NULL                              
REMARK 245   SAMPLE BUFFER                  : TRIS-HCL                          
REMARK 245   PH                             : 7.50                              
REMARK 245   SAMPLE DETAILS                 : NULL                              
REMARK 245                                                                      
REMARK 245 DATA ACQUISITION                                                     
REMARK 245   DATE OF EXPERIMENT                : 10-MAR-00                      
REMARK 245   NUMBER OF MICROGRAPHS-IMAGES      : NULL                           
REMARK 245   TEMPERATURE (KELVIN)              : 4.20                           
REMARK 245   MICROSCOPE MODEL                  : FEI/PHILIPS CM200FEG/SOPHIE    
REMARK 245   DETECTOR TYPE                     : KODAK SO-163 FILM              
REMARK 245   MINIMUM DEFOCUS (NM)              : 800.00                         
REMARK 245   MAXIMUM DEFOCUS (NM)              : 2500.00                        
REMARK 245   MINIMUM TILT ANGLE (DEGREES)      : 0.00                           
REMARK 245   MAXIMUM TILT ANGLE (DEGREES)      : 0.00                           
REMARK 245   NOMINAL CS                        : 1.35                           
REMARK 245   IMAGING MODE                      : BRIGHT FIELD                   
REMARK 245   ELECTRON DOSE (ELECTRONS NM**-2)  : 15.00                          
REMARK 245   ILLUMINATION MODE                 : FLOOD BEAM                     
REMARK 245   NOMINAL MAGNIFICATION             : NULL                           
REMARK 245   CALIBRATED MAGNIFICATION          : 58500                          
REMARK 245   SOURCE                            : FIELD EMISSION GUN             
REMARK 245   ACCELERATION VOLTAGE (KV)         : 120                            
REMARK 245   IMAGING DETAILS                   : NULL                           
REMARK 247                                                                      
REMARK 247 ELECTRON MICROSCOPY                                                  
REMARK 247  THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON          
REMARK 247  MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE              
REMARK 247  THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES           
REMARK 247  ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION         
REMARK 247  OF THE STRUCTURE FACTORS.                                           
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM                
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN                  
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON               
REMARK 300 BURIED SURFACE AREA.                                                 
REMARK 350                                                                      
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: OCTAMERIC                         
REMARK 350 APPLY THE FOLLOWING TO CHAINS: D, C, Q, R, A, O, P, L                
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     MET O    -3                                                      
REMARK 465     VAL O    -2                                                      
REMARK 465     ALA O    -1                                                      
REMARK 465     LEU O     0                                                      
REMARK 465     ALA O   125                                                      
REMARK 465     LYS O   126                                                      
REMARK 465     THR O   127                                                      
REMARK 465     ALA O   128                                                      
REMARK 465     ALA O   129                                                      
REMARK 465     LYS O   130                                                      
REMARK 465     LYS O   131                                                      
REMARK 465     MET P     0                                                      
REMARK 465     MET L    -6                                                      
REMARK 465     ALA L    -5                                                      
REMARK 465     LYS L    -4                                                      
REMARK 465     LYS L    -3                                                      
REMARK 465     VAL L    -2                                                      
REMARK 465     ALA L    -1                                                      
REMARK 465     ALA L     0                                                      
REMARK 465     ASP L   134                                                      
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: EMD-1004   RELATED DB: EMDB                              
DBREF  1MJ1 O    2   122  UNP    P0A7S3   RS12_ECOLI       2    122             
DBREF  1MJ1 D    1    76  PDB    1MJ1     1MJ1             1     76             
DBREF  1MJ1 C    1    76  PDB    1MJ1     1MJ1             1     76             
DBREF  1MJ1 Q    1    41  PDB    1MJ1     1MJ1             1     41             
DBREF  1MJ1 R    1    27  PDB    1MJ1     1MJ1             1     27             
DBREF  1MJ1 A    1   405  PDB    1MJ1     1MJ1             1    405             
DBREF  1MJ1 P    0   125  PDB    1MJ1     1MJ1             0    125             
DBREF  1MJ1 L   -6   134  PDB    1MJ1     1MJ1            -6    134             
SEQRES   1 D   76    G   C   G   G   A   U   U   U   A 2MG   C   U   C          
SEQRES   2 D   76    A   G H2U H2U   G   G   G   A   G   A   G   C M2G          
SEQRES   3 D   76    C   C   A   G   A OMC   U OMG   A   A  YG   A PSU          
SEQRES   4 D   76  5MC   U   G   G   A   G 7MG   U   C 5MC   U   G   U          
SEQRES   5 D   76    G 5MU PSU   C   G 1MA   U   C   C   A   C   A   G          
SEQRES   6 D   76    A   A   U   U   C   G   C   A   C   C   A                  
SEQRES   1 C   76    G   C   G   G   A   U   U   U   A 2MG   C   U   C          
SEQRES   2 C   76    A   G H2U H2U   G   G   G   A   G   A   G   C M2G          
SEQRES   3 C   76    C   C   A   G   A OMC   U OMG   A   A  YG   A PSU          
SEQRES   4 C   76  5MC   U   G   G   A   G 7MG   U   C 5MC   U   G   U          
SEQRES   5 C   76    G 5MU PSU   C   G 1MA   U   C   C   A   C   A   G          
SEQRES   6 C   76    A   A   U   U   C   G   C   A   C   C   A                  
SEQRES   1 Q   41    G   G   G   G   G   G   C   U   C   U   U   C   C          
SEQRES   2 Q   41    U   A   G   U   A   C   G   A   G   A   G   G   A          
SEQRES   3 Q   41    C   C   G   G   A   A   G   G   G   A   C   G   C          
SEQRES   4 Q   41    A   C                                                      
SEQRES   1 R   27    G   C   G   G   C   C   G   U   A   A   C   U   A          
SEQRES   2 R   27    U   A   A   C   G   G   U   C   C   U   A   A   G          
SEQRES   3 R   27    G                                                          
SEQRES   1 A  405  ALA LYS GLY GLU PHE ILE ARG THR LYS ARG HIS VAL ASN          
SEQRES   2 A  405  VAL GLY THR ILE GLY HIS VAL ASP HIS GLY LYS THR THR          
SEQRES   3 A  405  LEU THR ALA ALA LEU THR TYR VAL ALA ALA ALA GLU ASN          
SEQRES   4 A  405  ARG ASN VAL GLU VAL LYS ASP TYR GLY ASP ILE ASP LYS          
SEQRES   5 A  405  ALA ARG GLU GLU ARG ALA ARG GLY ILE THR ILE ASN THR          
SEQRES   6 A  405  ALA HIS VAL GLU TYR GLU THR ALA LYS ARG HIS TYR SER          
SEQRES   7 A  405  HIS VAL ASP CYS ARG GLY HIS ALA ASP TYR ILE LYS ASN          
SEQRES   8 A  405  MET ILE THR GLY ALA ALA GLN MET ASP GLY ALA ILE LEU          
SEQRES   9 A  405  VAL VAL SER ALA ALA ASP GLY ARG MET ARG GLN THR ARG          
SEQRES  10 A  405  GLU HIS ILE LEU LEU ALA ARG GLN VAL GLY VAL ARG TYR          
SEQRES  11 A  405  ILE VAL VAL PHE MET ASN LYS VAL ASP MET VAL ASP ASP          
SEQRES  12 A  405  ARG GLU LEU LEU ASP LEU VAL GLU MET GLU VAL ARG ASP          
SEQRES  13 A  405  LEU LEU ASN GLN TYR GLU PHE ARG GLY ASP GLU VAL ARG          
SEQRES  14 A  405  VAL ILE ARG GLY SER ALA LEU LEU ALA LEU GLU GLU MET          
SEQRES  15 A  405  HIS LYS ASN ARG LYS THR LYS ARG GLY GLU ASN GLU TRP          
SEQRES  16 A  405  VAL ASP LYS ILE TRP GLU LEU LEU ASP ALA ILE ASP GLU          
SEQRES  17 A  405  TYR ILE ARG THR ARG VAL ARG ASP VAL ASP LYS ARG PHE          
SEQRES  18 A  405  LEU MET ARG VAL GLU ASP VAL PHE THR ILE THR GLY ARG          
SEQRES  19 A  405  GLY THR VAL ALA THR GLY ARG ILE GLU ARG GLY LYS VAL          
SEQRES  20 A  405  LYS VAL GLY ASP GLU VAL GLU ILE VAL GLY LEU ALA ARG          
SEQRES  21 A  405  GLU THR ARG LYS THR VAL VAL THR GLY VAL GLU MET HIS          
SEQRES  22 A  405  ARG LYS THR LEU GLN GLU GLY ILE ALA GLY ASP ASN VAL          
SEQRES  23 A  405  GLY LEU LEU LEU ARG GLY VAL SER ARG GLU GLU VAL GLU          
SEQRES  24 A  405  ARG GLY GLN VAL LEU ALA LYS ARG GLY SER ILE THR ARG          
SEQRES  25 A  405  HIS THR LYS PHE GLU ALA SER VAL TYR ILE LEU LYS LYS          
SEQRES  26 A  405  GLU GLU GLY GLY ARG HIS THR GLY PHE PHE THR GLY TYR          
SEQRES  27 A  405  ARG ARG GLN PHE TYR PHE ARG THR THR ASP VAL THR GLY          
SEQRES  28 A  405  VAL VAL ARG LEU ARG GLN GLY VAL GLU MET VAL MET ARG          
SEQRES  29 A  405  GLY ASP ASN VAL THR PHE THR VAL GLU LEU ILE LYS ARG          
SEQRES  30 A  405  VAL ALA LEU GLU GLU GLY LEU ARG PHE ALA ILE ARG GLU          
SEQRES  31 A  405  GLY GLY ARG THR VAL GLY ALA GLY VAL VAL THR LYS ILE          
SEQRES  32 A  405  LEU GLU                                                      
SEQRES   1 O  135  MET VAL ALA LEU PRO THR ILE ASN GLN LEU VAL ARG LYS          
SEQRES   2 O  135  GLY ARG GLU LYS VAL ARG LYS LYS SER LYS VAL PRO ALA          
SEQRES   3 O  135  LEU LYS GLY ALA PRO PHE ARG ARG GLY VAL CYS THR VAL          
SEQRES   4 O  135  VAL ARG THR VAL THR PRO LYS LYS PRO ASN SER ALA LEU          
SEQRES   5 O  135  ARG LYS VAL ALA LYS VAL ARG LEU THR SER GLY TYR GLU          
SEQRES   6 O  135  VAL THR ALA TYR ILE PRO GLY GLU GLY HIS ASN LEU GLN          
SEQRES   7 O  135  GLU HIS SER VAL VAL LEU ILE ARG GLY GLY ARG VAL LYS          
SEQRES   8 O  135  ASP LEU PRO GLY VAL ARG TYR HIS ILE VAL ARG GLY VAL          
SEQRES   9 O  135  TYR ASP ALA ALA GLY VAL LYS ASP ARG LYS LYS SER ARG          
SEQRES  10 O  135  SER LYS TYR GLY THR LYS LYS PRO LYS GLU ALA ALA LYS          
SEQRES  11 O  135  THR ALA ALA LYS LYS                                          
SEQRES   1 P  126  MET ALA ARG ILE ALA GLY VAL GLU ILE PRO ARG ASN LYS          
SEQRES   2 P  126  ARG VAL ASP VAL ALA LEU THR TYR ILE TYR GLY ILE GLY          
SEQRES   3 P  126  LYS ALA ARG ALA LYS GLU ALA LEU GLU LYS THR GLY ILE          
SEQRES   4 P  126  ASN PRO ALA THR ARG VAL LYS ASP LEU THR GLU ALA GLU          
SEQRES   5 P  126  VAL VAL ARG LEU ARG GLU TYR VAL GLU ASN THR TRP LYS          
SEQRES   6 P  126  LEU GLU GLY GLU LEU ARG ALA GLU VAL ALA ALA ASN ILE          
SEQRES   7 P  126  LYS ARG LEU MET ASP ILE GLY CYS TYR ARG GLY LEU ARG          
SEQRES   8 P  126  HIS ARG ARG GLY LEU PRO VAL ARG GLY GLN ARG THR ARG          
SEQRES   9 P  126  THR ASN ALA ARG THR ARG LYS GLY PRO ARG LYS THR VAL          
SEQRES  10 P  126  ALA GLY LYS LYS LYS ALA PRO ARG LYS                          
SEQRES   1 L  141  MET ALA LYS LYS VAL ALA ALA GLN ILE LYS LEU GLN LEU          
SEQRES   2 L  141  PRO ALA GLY LYS ALA THR PRO ALA PRO PRO VAL GLY PRO          
SEQRES   3 L  141  ALA LEU GLY GLN HIS GLY VAL ASN ILE MET GLU PHE CYS          
SEQRES   4 L  141  LYS ARG PHE ASN ALA GLU THR ALA ASP LYS ALA GLY MET          
SEQRES   5 L  141  ILE LEU PRO VAL VAL ILE THR VAL TYR GLU ASP LYS SER          
SEQRES   6 L  141  PHE THR PHE ILE ILE LYS THR PRO PRO ALA SER PHE LEU          
SEQRES   7 L  141  LEU LYS LYS ALA ALA GLY ILE GLU LYS GLY SER SER GLU          
SEQRES   8 L  141  PRO LYS ARG LYS ILE VAL GLY LYS VAL THR ARG LYS GLN          
SEQRES   9 L  141  ILE GLU GLU ILE ALA LYS THR LYS MET PRO ASP LEU ASN          
SEQRES  10 L  141  ALA ASN SER LEU GLU ALA ALA MET LYS ILE ILE GLU GLY          
SEQRES  11 L  141  THR ALA LYS SER MET GLY ILE GLU VAL VAL ASP                  
MODRES 1MJ1 2MG D   10    G  2N-METHYLGUANOSINE-5'-MONOPHOSPHATE                
MODRES 1MJ1 H2U D   16    U  5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE                
MODRES 1MJ1 H2U D   17    U  5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE                
MODRES 1MJ1 M2G D   26    G  N2-DIMETHYLGUANOSINE-5'-MONOPHOSPHATE              
MODRES 1MJ1 OMC D   32    C  O2'-METHYLYCYTIDINE-5'-MONOPHOSPHATE               
MODRES 1MJ1 OMG D   34    G  O2'-METHYLGUANOSINE-5'-MONOPHOSPHATE               
MODRES 1MJ1  YG D   37    G  WYBUTOSINE                                         
MODRES 1MJ1 PSU D   39    U  PSEUDOURIDINE-5'-MONOPHOSPHATE                     
MODRES 1MJ1 5MC D   40    C  5-METHYLCYTIDINE-5'-MONOPHOSPHATE                  
MODRES 1MJ1 7MG D   46    G                                                     
MODRES 1MJ1 5MC D   49    C  5-METHYLCYTIDINE-5'-MONOPHOSPHATE                  
MODRES 1MJ1 5MU D   54    U  5-METHYLURIDINE 5'-MONOPHOSPHATE                   
MODRES 1MJ1 PSU D   55    U  PSEUDOURIDINE-5'-MONOPHOSPHATE                     
MODRES 1MJ1 1MA D   58    A                                                     
MODRES 1MJ1 2MG C   10    G  2N-METHYLGUANOSINE-5'-MONOPHOSPHATE                
MODRES 1MJ1 H2U C   16    U  5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE                
MODRES 1MJ1 H2U C   17    U  5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE                
MODRES 1MJ1 M2G C   26    G  N2-DIMETHYLGUANOSINE-5'-MONOPHOSPHATE              
MODRES 1MJ1 OMC C   32    C  O2'-METHYLYCYTIDINE-5'-MONOPHOSPHATE               
MODRES 1MJ1 OMG C   34    G  O2'-METHYLGUANOSINE-5'-MONOPHOSPHATE               
MODRES 1MJ1  YG C   37    G  WYBUTOSINE                                         
MODRES 1MJ1 PSU C   39    U  PSEUDOURIDINE-5'-MONOPHOSPHATE                     
MODRES 1MJ1 5MC C   40    C  5-METHYLCYTIDINE-5'-MONOPHOSPHATE                  
MODRES 1MJ1 7MG C   46    G                                                     
MODRES 1MJ1 5MC C   49    C  5-METHYLCYTIDINE-5'-MONOPHOSPHATE                  
MODRES 1MJ1 5MU C   54    U  5-METHYLURIDINE 5'-MONOPHOSPHATE                   
MODRES 1MJ1 PSU C   55    U  PSEUDOURIDINE-5'-MONOPHOSPHATE                     
MODRES 1MJ1 1MA C   58    A                                                     
HET    2MG  D  10      24                                                       
HET    H2U  D  16      20                                                       
HET    H2U  D  17      20                                                       
HET    M2G  D  26      25                                                       
HET    OMC  D  32      21                                                       
HET    OMG  D  34      24                                                       
HET     YG  D  37      39                                                       
HET    PSU  D  39      20                                                       
HET    5MC  D  40      21                                                       
HET    7MG  D  46      24                                                       
HET    5MC  D  49      21                                                       
HET    5MU  D  54      21                                                       
HET    PSU  D  55      20                                                       
HET    1MA  D  58      23                                                       
HET    2MG  C  10      24                                                       
HET    H2U  C  16      20                                                       
HET    H2U  C  17      20                                                       
HET    M2G  C  26      25                                                       
HET    OMC  C  32      21                                                       
HET    OMG  C  34      24                                                       
HET     YG  C  37      39                                                       
HET    PSU  C  39      20                                                       
HET    5MC  C  40      21                                                       
HET    7MG  C  46      24                                                       
HET    5MC  C  49      21                                                       
HET    5MU  C  54      21                                                       
HET    PSU  C  55      20                                                       
HET    1MA  C  58      23                                                       
HETNAM     2MG 2N-METHYLGUANOSINE-5'-MONOPHOSPHATE                              
HETNAM     H2U 5,6-DIHYDROURIDINE-5'-MONOPHOSPHATE                              
HETNAM     M2G N2-DIMETHYLGUANOSINE-5'-MONOPHOSPHATE                            
HETNAM     OMC O2'-METHYLYCYTIDINE-5'-MONOPHOSPHATE                             
HETNAM     OMG O2'-METHYLGUANOSINE-5'-MONOPHOSPHATE                             
HETNAM      YG WYBUTOSINE                                                       
HETNAM     PSU PSEUDOURIDINE-5'-MONOPHOSPHATE                                   
HETNAM     5MC 5-METHYLCYTIDINE-5'-MONOPHOSPHATE                                
HETNAM     7MG 7N-METHYL-8-HYDROGUANOSINE-5'-MONOPHOSPHATE                      
HETNAM     5MU 5-METHYLURIDINE 5'-MONOPHOSPHATE                                 
HETNAM     1MA 6-HYDRO-1-METHYLADENOSINE-5'-MONOPHOSPHATE                       
HETSYN      YG Y-BASE; 1H-IMIDAZO(1,2-ALPHA)PURINE-7-BUTANOIC ACID,4,           
HETSYN   2  YG  9-DIHYDRO-ALPHA-((METHOXYCARBONYL)AMINO)-4,6-DIMETHYL-          
HETSYN   3  YG  9-OXO-METHYL ESTER                                              
FORMUL   1  2MG    2(C11 H16 N5 O8 P)                                           
FORMUL   1  H2U    4(C9 H15 N2 O9 P)                                            
FORMUL   1  M2G    2(C12 H18 N5 O8 P)                                           
FORMUL   1  OMC    2(C10 H16 N3 O8 P)                                           
FORMUL   1  OMG    2(C11 H16 N5 O8 P)                                           
FORMUL   1   YG    2(C21 H29 N6 O12 P)                                          
FORMUL   1  PSU    4(C9 H13 N2 O9 P)                                            
FORMUL   1  5MC    4(C10 H16 N3 O8 P)                                           
FORMUL   1  7MG    2(C11 H18 N5 O8 P)                                           
FORMUL   1  5MU    2(C10 H15 N2 O9 P)                                           
FORMUL   1  1MA    2(C11 H16 N5 O7 P)                                           
HELIX    1   1 GLY A   23  ASN A   39  1                                  17    
HELIX    2   2 ASP A   46  ASP A   51  1                                   6    
HELIX    3   3 ALA A   53  ARG A   59  1                                   7    
HELIX    4   4 HIS A   85  ASP A   87  5                                   3    
HELIX    5   5 TYR A   88  ALA A   97  1                                  10    
HELIX    6   6 MET A  113  VAL A  126  1                                  14    
HELIX    7   7 LYS A  137  VAL A  141  5                                   5    
HELIX    8   8 ASP A  143  TYR A  161  1                                  19    
HELIX    9   9 SER A  174  ARG A  186  1                                  13    
HELIX   10  10 ASN A  193  TYR A  209  1                                  17    
HELIX   11  11 SER A  294  VAL A  298  5                                   5    
HELIX   12  12 LYS A  324  GLY A  328  5                                   5    
SHEET    1   A 6 ALA A  66  GLU A  71  0                                        
SHEET    2   A 6 ARG A  75  ASP A  81 -1  O  HIS A  79   N  VAL A  68           
SHEET    3   A 6 ARG A  10  ILE A  17  1  N  ARG A  10   O  HIS A  76           
SHEET    4   A 6 ALA A 102  SER A 107  1  O  VAL A 105   N  ILE A  17           
SHEET    5   A 6 ILE A 131  ASN A 136  1  O  VAL A 132   N  LEU A 104           
SHEET    6   A 6 VAL A 170  ARG A 172  1  O  ILE A 171   N  MET A 135           
SHEET    1   B 7 LEU A 222  ILE A 231  0                                        
SHEET    2   B 7 GLY A 235  ARG A 244 -1  O  GLU A 243   N  LEU A 222           
SHEET    3   B 7 ASN A 285  LEU A 290 -1  O  VAL A 286   N  GLY A 240           
SHEET    4   B 7 ARG A 263  GLU A 271 -1  N  GLU A 271   O  GLY A 287           
SHEET    5   B 7 GLU A 252  VAL A 256 -1  N  VAL A 253   O  THR A 265           
SHEET    6   B 7 VAL A 303  ALA A 305 -1  O  VAL A 303   N  VAL A 256           
SHEET    7   B 7 LEU A 222  ILE A 231 -1  N  MET A 223   O  LEU A 304           
SHEET    1   C 2 LYS A 246  LYS A 248  0                                        
SHEET    2   C 2 GLU A 279  ILE A 281 -1  O  GLY A 280   N  VAL A 247           
SHEET    1   D 7 HIS A 313  ILE A 322  0                                        
SHEET    2   D 7 ASN A 367  VAL A 378 -1  O  VAL A 378   N  HIS A 313           
SHEET    3   D 7 THR A 347  LEU A 355 -1  N  ARG A 354   O  THR A 371           
SHEET    4   D 7 GLN A 341  PHE A 344 -1  N  PHE A 342   O  VAL A 349           
SHEET    5   D 7 ARG A 385  GLU A 390 -1  O  ALA A 387   N  TYR A 343           
SHEET    6   D 7 ARG A 393  ILE A 403 -1  O  ARG A 393   N  GLU A 390           
SHEET    7   D 7 HIS A 313  ILE A 322 -1  N  GLU A 317   O  LYS A 402           
LINK         O3'   A D   9                 P   2MG D  10     1555   1555  1.61  
LINK         O3' 2MG D  10                 P     C D  11     1555   1555  1.61  
LINK         O3'   G D  15                 P   H2U D  16     1555   1555  1.61  
LINK         O3' H2U D  16                 P   H2U D  17     1555   1555  1.62  
LINK         O3' H2U D  17                 P     G D  18     1555   1555  1.60  
LINK         O3'   C D  25                 P   M2G D  26     1555   1555  1.62  
LINK         O3' M2G D  26                 P     C D  27     1555   1555  1.61  
LINK         O3'   A D  31                 P   OMC D  32     1555   1555  1.61  
LINK         O3' OMC D  32                 P     U D  33     1555   1555  1.60  
LINK         O3'   U D  33                 P   OMG D  34     1555   1555  1.62  
LINK         O3' OMG D  34                 P     A D  35     1555   1555  1.62  
LINK         O3'   A D  36                 P    YG D  37     1555   1555  1.64  
LINK         O3'  YG D  37                 P     A D  38     1555   1555  1.61  
LINK         O3'   A D  38                 P   PSU D  39     1555   1555  1.62  
LINK         O3' PSU D  39                 P   5MC D  40     1555   1555  1.61  
LINK         O3' 5MC D  40                 P     U D  41     1555   1555  1.61  
LINK         O3'   G D  45                 P   7MG D  46     1555   1555  1.61  
LINK         O3' 7MG D  46                 P     U D  47     1555   1555  1.62  
LINK         O3'   C D  48                 P   5MC D  49     1555   1555  1.62  
LINK         O3' 5MC D  49                 P     U D  50     1555   1555  1.62  
LINK         O3'   G D  53                 P   5MU D  54     1555   1555  1.63  
LINK         O3' 5MU D  54                 P   PSU D  55     1555   1555  1.62  
LINK         O3' PSU D  55                 P     C D  56     1555   1555  1.62  
LINK         O3'   G D  57                 P   1MA D  58     1555   1555  1.61  
LINK         O3' 1MA D  58                 P     U D  59     1555   1555  1.60  
LINK         O3'   A C   9                 P   2MG C  10     1555   1555  1.63  
LINK         O3' 2MG C  10                 P     C C  11     1555   1555  1.60  
LINK         O3'   G C  15                 P   H2U C  16     1555   1555  1.61  
LINK         O3' H2U C  16                 P   H2U C  17     1555   1555  1.62  
LINK         O3' H2U C  17                 P     G C  18     1555   1555  1.60  
LINK         O3' M2G C  26                 P     C C  27     1555   1555  1.60  
LINK         O3'   A C  31                 P   OMC C  32     1555   1555  1.61  
LINK         O3' OMC C  32                 P     U C  33     1555   1555  1.61  
LINK         O3'   U C  33                 P   OMG C  34     1555   1555  1.41  
LINK         O3' OMG C  34                 P     A C  35     1555   1555  1.59  
LINK         O3'   A C  36                 P    YG C  37     1555   1555  1.18  
LINK         O3'  YG C  37                 P     A C  38     1555   1555  1.61  
LINK         O3'   A C  38                 P   PSU C  39     1555   1555  1.61  
LINK         O3' PSU C  39                 P   5MC C  40     1555   1555  1.61  
LINK         O3' 5MC C  40                 P     U C  41     1555   1555  1.60  
LINK         O3'   G C  45                 P   7MG C  46     1555   1555  1.60  
LINK         O3' 7MG C  46                 P     U C  47     1555   1555  1.63  
LINK         O3'   C C  48                 P   5MC C  49     1555   1555  1.60  
LINK         O3' 5MC C  49                 P     U C  50     1555   1555  1.62  
LINK         O3'   G C  53                 P   5MU C  54     1555   1555  1.64  
LINK         O3' 5MU C  54                 P   PSU C  55     1555   1555  1.62  
LINK         O3' PSU C  55                 P     C C  56     1555   1555  1.61  
LINK         O3'   G C  57                 P   1MA C  58     1555   1555  1.59  
LINK         O3' 1MA C  58                 P     U C  59     1555   1555  1.64  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system