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Database: PDB
Entry: 1MX8
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Original site: 1MX8 
HEADER    LIPID BINDING PROTEIN                   01-OCT-02   1MX8              
TITLE     TWO HOMOLOGOUS RAT CELLULAR RETINOL-BINDING PROTEINS DIFFER           
TITLE    2 IN LOCAL STRUCTURE AND FLEXIBILITY                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CELLULAR RETINOL-BINDING PROTEIN I, HOLO;                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: RETINOL BINDING PROTEIN -I (CELLULAR), CRBP;                
COMPND   5 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: RATTUS NORVEGICUS;                              
SOURCE   3 ORGANISM_COMMON: NORWAY RAT;                                         
SOURCE   4 ORGANISM_TAXID: 10116;                                               
SOURCE   5 GENE: RBP1 OR RBP-1;                                                 
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: JM101;                                     
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PMON CRBP I                               
KEYWDS    BETA-BARREL, HELIX-TURN-HELIX, VITAMIN A, RETONOL BINDING,            
KEYWDS   2 TRANSPORT, LIPID BINDING PROTEIN                                     
EXPDTA    SOLUTION NMR                                                          
NUMMDL    25                                                                    
MDLTYP    MINIMIZED AVERAGE                                                     
AUTHOR    J.LU,D.P.CISTOLA,E.LI                                                 
REVDAT   3   24-FEB-09 1MX8    1       VERSN                                    
REVDAT   2   02-DEC-03 1MX8    1       DBREF                                    
REVDAT   1   29-JUL-03 1MX8    0                                                
JRNL        AUTH   J.LU,D.P.CISTOLA,E.LI                                        
JRNL        TITL   TWO HOMOLOGOUS RAT CELLULAR RETINOL-BINDING                  
JRNL        TITL 2 PROTEINS DIFFER IN LOCAL CONFORMATIONAL                      
JRNL        TITL 3 FLEXIBILITY.                                                 
JRNL        REF    J.MOL.BIOL.                   V. 330   799 2003              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   12850148                                                     
JRNL        DOI    10.1016/S0022-2836(03)00629-6                                
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   J.LU,C.L.LIN,C.TANG,J.W.PONDER,J.L.KAO,D.P.CISTOLA,          
REMARK   1  AUTH 2 E.LI                                                         
REMARK   1  TITL   BINDING OF RETINOL INDUCES CHANGES IN RAT CELLULAR           
REMARK   1  TITL 2 RETINOL-BINDING PROTEIN II CONFORMATION AND                  
REMARK   1  TITL 3 BACKBONE DYNAMICS                                            
REMARK   1  REF    J.MOL.BIOL.                   V. 300   619 2000              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   1  DOI    10.1006/JMBI.2000.3883                                       
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   J.LU,C.L.LIN,C.TANG,J.W.PONDER,J.L.F.KAO,                    
REMARK   1  AUTH 2 D.P.CISTOLA,E.LI                                             
REMARK   1  TITL   THE STRUCTURE AND DYNAMICS OF RAT APO-CELLULAR               
REMARK   1  TITL 2 RETINOL-BINDING PROTEIN II IN SOLUTION: COMPARISON           
REMARK   1  TITL 3 WITH THE X-RAY STRUCTURE                                     
REMARK   1  REF    J.MOL.BIOL.                   V. 286  1179 1999              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   1  DOI    10.1006/JMBI.1999.2544                                       
REMARK   1 REFERENCE 3                                                          
REMARK   1  AUTH   S.W.COWAN,M.E.NEWCOMER,T.A.JONES                             
REMARK   1  TITL   CRYSTALLOGRAPHIC STUDIES ON A FAMILY OF CELLULAR             
REMARK   1  TITL 2 LIPOPHILIC TRANSPORT PROTEINS. REFINEMENT OF P2              
REMARK   1  TITL 3 MYELIN PROTEIN AND THE STRUCTURE DETERMINATION AND           
REMARK   1  TITL 4 REFINEMENT OF CELLULAR RETINOL-BINDING PROTEIN IN            
REMARK   1  TITL 5 COMPLEX WITH ALL-TRANS-RETINOL                               
REMARK   1  REF    J.MOL.BIOL.                   V. 230  1225 1993              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   1  DOI    10.1006/JMBI.1993.1238                                       
REMARK   1 REFERENCE 4                                                          
REMARK   1  AUTH   N.S.WINTER,J.M.BRATT,L.J.BANASZAK                            
REMARK   1  TITL   CRYSTAL STRUCTURES OF HOLO AND APO-CELLULAR                  
REMARK   1  TITL 2 RETINOL-BINDING PROTEIN II                                   
REMARK   1  REF    J.MOL.BIOL.                   V. 230  1247 1993              
REMARK   1  REFN                   ISSN 0022-2836                               
REMARK   1  DOI    10.1006/JMBI.1993.1239                                       
REMARK   1 REFERENCE 5                                                          
REMARK   1  AUTH   L.FRANZONI,C.LUCKE,C.PEREZ,D.CAVAZZINI,                      
REMARK   1  AUTH 2 M.RADEMACHER,C.LUDWIG,A.SPISNI,G.L.ROSSI,                    
REMARK   1  AUTH 3 H.RUTERJANS                                                  
REMARK   1  TITL   STRUCTURE AND BACKBONE DYNAMICS OF APO- AND                  
REMARK   1  TITL 2 HOLO-CELLULAR RETINAL-BINDING PROTEIN IN SOLUTION            
REMARK   1  REF    J.BIOL.CHEM.                  V. 277 21983 2002              
REMARK   1  REFN                   ISSN 0021-9258                               
REMARK   1  DOI    10.1074/JBC.M201994200                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : TINKER 3.3                                           
REMARK   3   AUTHORS     : PONDER, J.W.                                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1MX8 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 04-OCT-02.                  
REMARK 100 THE RCSB ID CODE IS RCSB017273.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298; 298                           
REMARK 210  PH                             : 7.4; 6.5                           
REMARK 210  IONIC STRENGTH                 : 0.34; 0.34                         
REMARK 210  PRESSURE                       : AMBIENT; AMBIENT                   
REMARK 210  SAMPLE CONTENTS                : 1.0MM CELLULAR RETINOL-            
REMARK 210                                   BINDING PROTEIN I U-[99% 15N,      
REMARK 210                                   99% 13C] IN COMPLEX WITH ALL-      
REMARK 210                                   TRANS RETINOL (NATURAL ISOTOPE     
REMARK 210                                   ABUNDANCE); 20MM PHOSPHATE         
REMARK 210                                   BUFFER, 50MM POTASSIUM             
REMARK 210                                   CHLORIDE, 0.05% SUDIUM AZIDE,      
REMARK 210                                   5MM BETA-MECAPTOETHANOL-D6;        
REMARK 210                                   95% H2O, 5% D2O; 1.0MM             
REMARK 210                                   CELLULAR RETINOL-BINDING           
REMARK 210                                   PROTEIN I U-[99% 15N, 80% 2H]      
REMARK 210                                   IN COMPLEX WITH ALL-TRANS          
REMARK 210                                   RETINOL (NATURAL ISOTOPE           
REMARK 210                                   ABUNDANCE); 20MM PHOSPHATE         
REMARK 210                                   BUFFER, 50MM POTASSIUM             
REMARK 210                                   CHLORIDE, 0.05% SUDIUM AZIDE,      
REMARK 210                                   5MM BETA-MECAPTOETHANOL-D6;        
REMARK 210                                   95% H2O, 5% D2O                    
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : INOVA, UNITYPLUS                   
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1, FELIX 2000, TINKER       
REMARK 210                                   3.3                                
REMARK 210   METHOD USED                   : DISTANCE GEOMETRY   SIMULATED      
REMARK 210                                   ANNEALING                          
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 50                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 25                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : TARGET FUNCTION                    
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: TRIPLE RESONANCE EXPERIMENTS FOR    THE 1H, 15N AND 13C      
REMARK 210  RESONANCE ASSIGNMENTS INCLUDED THE FOLLOWING: HNCO CBCACONNH        
REMARK 210  HNCACB CBCACOCAHA HCCH-TOCSY 15N-RESOLVED TOCSY-HSQC; THE           
REMARK 210  INTRA-LIGAND NOE CONSTRAINTS AS WELL AS LIGAND-PROTEIN NOE          
REMARK 210  CONSTRAINTS WERE OBTAINED FROM 2D 13C AND 15N DOUBLE HALF-          
REMARK 210  FILTERED EXPERIMENT                                                 
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500 12 TRP A  88   CE2   TRP A  88   CD2    -0.073                       
REMARK 500 20 TRP A 106   CE2   TRP A 106   CD2    -0.072                       
REMARK 500 21 TRP A 106   CE2   TRP A 106   CD2    -0.073                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 TYR A   7   CB  -  CG  -  CD2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  1 TYR A   7   CB  -  CG  -  CD1 ANGL. DEV. =   4.3 DEGREES          
REMARK 500  1 TRP A   8   CG  -  CD2 -  CE3 ANGL. DEV. =  -5.4 DEGREES          
REMARK 500  1 ARG A  21   NE  -  CZ  -  NH2 ANGL. DEV. =  -5.4 DEGREES          
REMARK 500  2 TRP A   8   CE2 -  CD2 -  CG  ANGL. DEV. =   5.1 DEGREES          
REMARK 500  2 TRP A   8   CG  -  CD2 -  CE3 ANGL. DEV. =  -5.7 DEGREES          
REMARK 500  2 GLU A  18   OE1 -  CD  -  OE2 ANGL. DEV. =   7.2 DEGREES          
REMARK 500  2 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500  2 ARG A  58   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.0 DEGREES          
REMARK 500  2 TYR A  60   CB  -  CG  -  CD2 ANGL. DEV. =  -3.6 DEGREES          
REMARK 500  3 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  3 PHE A  64   CB  -  CG  -  CD2 ANGL. DEV. =  -4.7 DEGREES          
REMARK 500  4 ARG A  21   NE  -  CZ  -  NH1 ANGL. DEV. =   4.4 DEGREES          
REMARK 500  4 ARG A  21   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  4 ARG A  52   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500  4 ARG A 104   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500  5 TRP A   8   CD1 -  CG  -  CD2 ANGL. DEV. =   6.2 DEGREES          
REMARK 500  5 ARG A  52   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500  5 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500  5 ARG A 120   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  6 TYR A  19   CB  -  CG  -  CD1 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  6 ARG A  58   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.8 DEGREES          
REMARK 500  7 ARG A  21   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  7 ARG A  21   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.2 DEGREES          
REMARK 500  7 ARG A  30   NE  -  CZ  -  NH2 ANGL. DEV. =   3.8 DEGREES          
REMARK 500  7 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500  7 ARG A 120   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500  7 ARG A 120   NE  -  CZ  -  NH2 ANGL. DEV. =  -4.2 DEGREES          
REMARK 500  8 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.3 DEGREES          
REMARK 500  8 ARG A  80   NE  -  CZ  -  NH1 ANGL. DEV. =   4.1 DEGREES          
REMARK 500  8 ARG A 104   NE  -  CZ  -  NH1 ANGL. DEV. =   3.7 DEGREES          
REMARK 500  9 ARG A  30   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  9 ARG A 104   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.1 DEGREES          
REMARK 500 10 TRP A   8   CG  -  CD2 -  CE3 ANGL. DEV. =  -5.6 DEGREES          
REMARK 500 10 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500 10 ARG A  80   NE  -  CZ  -  NH1 ANGL. DEV. =   3.7 DEGREES          
REMARK 500 10 ARG A  80   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.4 DEGREES          
REMARK 500 11 TYR A  19   CB  -  CG  -  CD1 ANGL. DEV. =  -4.1 DEGREES          
REMARK 500 11 ARG A  52   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500 11 ARG A  58   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 11 TRP A 106   CD1 -  CG  -  CD2 ANGL. DEV. =   5.1 DEGREES          
REMARK 500 11 GLU A 111   OE1 -  CD  -  OE2 ANGL. DEV. =   7.3 DEGREES          
REMARK 500 12 ARG A  21   NE  -  CZ  -  NH1 ANGL. DEV. =   3.3 DEGREES          
REMARK 500 12 ARG A  30   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500 12 ARG A  30   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.2 DEGREES          
REMARK 500 12 ARG A  52   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500 12 TRP A 109   CE2 -  CD2 -  CG  ANGL. DEV. =   5.6 DEGREES          
REMARK 500 13 ARG A  21   NE  -  CZ  -  NH1 ANGL. DEV. =   4.5 DEGREES          
REMARK 500 13 ARG A  30   NE  -  CZ  -  NH1 ANGL. DEV. =   4.1 DEGREES          
REMARK 500 13 ARG A  30   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.3 DEGREES          
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     106 ANGLE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASN A   5       87.46    -55.04                                   
REMARK 500  1 ASN A  15       32.34   -144.40                                   
REMARK 500  1 ASN A  26      160.73    -49.38                                   
REMARK 500  1 LYS A  37       76.15   -167.19                                   
REMARK 500  1 ASP A  45       72.41   -160.60                                   
REMARK 500  1 ILE A  77      -69.66   -100.45                                   
REMARK 500  1 ASP A  78       22.18   -149.14                                   
REMARK 500  1 ASP A  79       78.75     52.20                                   
REMARK 500  1 LYS A  98       49.46    -94.43                                   
REMARK 500  1 LYS A 101     -171.27     69.44                                   
REMARK 500  1 ARG A 104       93.96    -50.42                                   
REMARK 500  1 TRP A 109      108.19   -169.23                                   
REMARK 500  1 ALA A 121      116.23   -161.97                                   
REMARK 500  2 LEU A  11      -67.14   -106.65                                   
REMARK 500  2 GLU A  14      -48.56   -139.43                                   
REMARK 500  2 ASP A  47       31.29     71.28                                   
REMARK 500  2 SER A  55      -28.55   -162.81                                   
REMARK 500  2 THR A  56      -15.49     94.18                                   
REMARK 500  2 ARG A  58      132.25   -177.75                                   
REMARK 500  2 ASP A  73       99.05    -63.78                                   
REMARK 500  2 ILE A  77      -72.04   -154.22                                   
REMARK 500  2 LYS A  98      -89.55    -88.65                                   
REMARK 500  2 GLU A 100       66.40   -155.68                                   
REMARK 500  2 ARG A 104       91.49     54.63                                   
REMARK 500  2 TRP A 109       79.24   -169.13                                   
REMARK 500  2 ALA A 121      111.11   -165.52                                   
REMARK 500  3 LEU A  11      -56.53   -136.17                                   
REMARK 500  3 GLU A  14      -58.03   -141.64                                   
REMARK 500  3 ASP A  24       61.78     76.57                                   
REMARK 500  3 PRO A  38     -179.11    -59.97                                   
REMARK 500  3 ARG A  58      144.98   -170.17                                   
REMARK 500  3 ASP A  63       57.22    -90.53                                   
REMARK 500  3 ILE A  77      -85.23   -112.77                                   
REMARK 500  3 ASP A  79       89.09     40.94                                   
REMARK 500  3 ARG A  80       46.44   -152.68                                   
REMARK 500  3 LYS A  81      120.32    -39.56                                   
REMARK 500  3 ASP A  89       57.43   -147.66                                   
REMARK 500  3 LYS A  98       58.35   -143.24                                   
REMARK 500  3 GLU A 100     -159.56     63.04                                   
REMARK 500  3 LYS A 101     -175.44     68.37                                   
REMARK 500  3 GLU A 102      101.15   -164.01                                   
REMARK 500  3 TRP A 109      -62.02   -140.06                                   
REMARK 500  3 ILE A 110      142.08     70.26                                   
REMARK 500  3 ASP A 113       69.66   -154.17                                   
REMARK 500  3 GLU A 114      130.49    164.60                                   
REMARK 500  3 ALA A 121      128.65   -170.06                                   
REMARK 500  3 VAL A 133      -71.91   -115.22                                   
REMARK 500  4 MET A  10      168.77    -49.53                                   
REMARK 500  4 LEU A  11      -56.62   -142.51                                   
REMARK 500  4 SER A  12      147.79   -170.70                                   
REMARK 500  4 ASP A  24       43.01     35.62                                   
REMARK 500  4 ASN A  26      153.05    -45.99                                   
REMARK 500  4 PRO A  38     -171.86    -67.76                                   
REMARK 500  4 HIS A  48       80.06    158.80                                   
REMARK 500  4 PHE A  57      -68.50   -127.48                                   
REMARK 500  4 ASN A  59       93.73    -31.56                                   
REMARK 500  4 THR A  75       99.48    -58.75                                   
REMARK 500  4 ILE A  77      -65.59   -106.91                                   
REMARK 500  4 ASP A  79       90.45     48.43                                   
REMARK 500  4 ARG A  80      123.25   -177.25                                   
REMARK 500  4 THR A 107      117.55   -160.54                                   
REMARK 500  4 ASP A 113       71.54   -114.71                                   
REMARK 500  4 GLU A 114      140.66    172.69                                   
REMARK 500  5 LEU A  11      -60.74    -94.98                                   
REMARK 500  5 GLU A  14      114.84   -173.70                                   
REMARK 500  5 ASN A  26      153.82    -45.44                                   
REMARK 500  5 LYS A  37       68.13    175.94                                   
REMARK 500  5 PRO A  38      170.60    -56.23                                   
REMARK 500  5 ASP A  45       66.15   -156.23                                   
REMARK 500  5 ASP A  47      -58.78     72.85                                   
REMARK 500  5 MET A  49       89.81   -152.90                                   
REMARK 500  5 GLN A  65       99.06     60.36                                   
REMARK 500  5 GLU A  71       77.77   -116.83                                   
REMARK 500  5 ILE A  77      -68.68   -101.70                                   
REMARK 500  5 LYS A  81      133.03   -176.81                                   
REMARK 500  5 ALA A 121      133.70    165.40                                   
REMARK 500  5 VAL A 133      -52.61   -134.16                                   
REMARK 500  6 SER A  12     -179.00   -171.57                                   
REMARK 500  6 ASP A  47      -66.13     70.56                                   
REMARK 500  6 PHE A  57      -71.45   -143.85                                   
REMARK 500  6 ASN A  59      172.74     62.30                                   
REMARK 500  6 TYR A  60      105.71    177.30                                   
REMARK 500  6 LEU A  74       56.90   -113.32                                   
REMARK 500  6 ILE A  77      -61.71   -132.51                                   
REMARK 500  6 ASP A  78       15.30   -143.97                                   
REMARK 500  6 ASP A  79       87.97     37.16                                   
REMARK 500  6 LYS A  81      107.34     68.99                                   
REMARK 500  6 ASP A  91      -74.44     66.89                                   
REMARK 500  6 ARG A 104       69.95     25.72                                   
REMARK 500  6 TRP A 109       96.53   -161.42                                   
REMARK 500  6 GLU A 111       97.43   -160.20                                   
REMARK 500  6 ALA A 121      105.90   -167.59                                   
REMARK 500  6 LYS A 132      108.42    -55.25                                   
REMARK 500  7 VAL A   2      104.78    -31.73                                   
REMARK 500  7 MET A  10      179.99    -55.11                                   
REMARK 500  7 LEU A  11      -51.98   -162.37                                   
REMARK 500  7 THR A  56      -61.58     72.48                                   
REMARK 500  7 LEU A  74       62.05   -102.94                                   
REMARK 500  7 ILE A  77      -60.40   -151.06                                   
REMARK 500  7 ASP A  79       95.24     51.59                                   
REMARK 500  7 ARG A  80      128.08    178.08                                   
REMARK 500  7 ASP A  89      -57.53   -123.57                                   
REMARK 500  7 LYS A 101      179.11     68.92                                   
REMARK 500  7 ARG A 104      130.33    -39.89                                   
REMARK 500  7 TRP A 109      119.69   -170.06                                   
REMARK 500  7 ALA A 121      121.90   -176.27                                   
REMARK 500  8 VAL A   2       23.89   -144.74                                   
REMARK 500  8 ASP A   3      104.27    -39.97                                   
REMARK 500  8 GLU A  14      -53.48   -147.92                                   
REMARK 500  8 ASP A  24       84.31     58.97                                   
REMARK 500  8 LYS A  37       63.25   -152.72                                   
REMARK 500  8 ASP A  45       75.79   -151.98                                   
REMARK 500  8 ASP A  47       49.78   -104.06                                   
REMARK 500  8 HIS A  48      111.11   -175.42                                   
REMARK 500  8 THR A  56        9.61     93.87                                   
REMARK 500  8 ARG A  58       49.38   -156.20                                   
REMARK 500  8 ASN A  59      107.24    -10.57                                   
REMARK 500  8 LEU A  74       37.23    -97.04                                   
REMARK 500  8 ILE A  77      -60.84   -134.75                                   
REMARK 500  8 ASP A  78       20.72   -156.81                                   
REMARK 500  8 ASP A  79       81.47     37.92                                   
REMARK 500  8 LYS A  81      107.22     72.99                                   
REMARK 500  8 GLU A 100       83.71     45.28                                   
REMARK 500  8 GLU A 114       93.88   -171.84                                   
REMARK 500  8 ALA A 121      128.47    162.09                                   
REMARK 500  8 VAL A 133      -54.34   -136.03                                   
REMARK 500  9 LEU A  11      -48.55   -130.28                                   
REMARK 500  9 ASN A  13     -165.90   -116.11                                   
REMARK 500  9 GLU A  14      -54.84   -162.30                                   
REMARK 500  9 ASN A  15       25.24   -141.32                                   
REMARK 500  9 ASN A  26      140.94    -35.86                                   
REMARK 500  9 ASP A  45       46.76   -144.35                                   
REMARK 500  9 MET A  49      104.57   -165.35                                   
REMARK 500  9 ARG A  58      134.99   -179.95                                   
REMARK 500  9 ASP A  73       96.65    -54.18                                   
REMARK 500  9 LEU A  74       47.33   -101.00                                   
REMARK 500  9 ILE A  77      -74.08   -127.96                                   
REMARK 500  9 ASP A  79       70.26     28.97                                   
REMARK 500  9 LYS A  81      115.93    -31.38                                   
REMARK 500  9 ASP A  91      -86.41     62.22                                   
REMARK 500  9 LYS A  98      -71.47   -111.63                                   
REMARK 500  9 ARG A 104       92.24     48.43                                   
REMARK 500  9 TRP A 109      109.28   -162.23                                   
REMARK 500  9 GLU A 118       77.33   -108.82                                   
REMARK 500  9 ALA A 121      122.24   -179.13                                   
REMARK 500 10 ASP A   3       99.67     58.19                                   
REMARK 500 10 LEU A  11      -52.76   -120.72                                   
REMARK 500 10 GLU A  14      -57.18   -146.23                                   
REMARK 500 10 ASN A  26      157.28    -48.61                                   
REMARK 500 10 ARG A  58      105.07   -168.51                                   
REMARK 500 10 LEU A  74       48.37   -105.13                                   
REMARK 500 10 ILE A  77      -63.43   -137.80                                   
REMARK 500 10 ASP A  78       22.99   -153.89                                   
REMARK 500 10 ASP A  79       90.70     39.88                                   
REMARK 500 10 ARG A  80      -61.69   -129.75                                   
REMARK 500 10 LYS A  81      103.96     67.19                                   
REMARK 500 10 ASP A  89       44.12     30.97                                   
REMARK 500 10 ASP A  91      121.78    153.38                                   
REMARK 500 10 LYS A  92      115.92     75.03                                   
REMARK 500 10 GLU A 100       85.28    -56.20                                   
REMARK 500 10 ARG A 104       94.19    -31.20                                   
REMARK 500 10 ASP A 113       74.02     62.19                                   
REMARK 500 10 GLU A 114      136.64    173.18                                   
REMARK 500 10 ALA A 121      117.37   -171.39                                   
REMARK 500 11 LEU A  11      -58.11   -138.35                                   
REMARK 500 11 ASN A  13       93.35   -175.44                                   
REMARK 500 11 VAL A  25      -74.74    -57.72                                   
REMARK 500 11 ASN A  26      150.52    172.16                                   
REMARK 500 11 LYS A  37       80.07   -151.52                                   
REMARK 500 11 MET A  49       84.12   -155.41                                   
REMARK 500 11 THR A  56      -35.91    -33.24                                   
REMARK 500 11 TYR A  60       89.97   -163.27                                   
REMARK 500 11 ILE A  77      -74.78   -114.31                                   
REMARK 500 11 ASP A  78       20.49   -143.77                                   
REMARK 500 11 ASP A  79       76.90     36.31                                   
REMARK 500 11 LYS A  81       85.22     71.69                                   
REMARK 500 11 LYS A  92      143.04   -172.85                                   
REMARK 500 11 GLN A  97       79.86   -104.75                                   
REMARK 500 11 LYS A  98       94.79    -41.51                                   
REMARK 500 11 GLU A 100       46.11   -142.47                                   
REMARK 500 11 LYS A 101      -89.34   -124.71                                   
REMARK 500 11 GLU A 102      136.26    171.53                                   
REMARK 500 11 GLU A 114      146.04    177.12                                   
REMARK 500 11 GLU A 118       73.18   -118.91                                   
REMARK 500 11 ALA A 121      118.44   -160.78                                   
REMARK 500 11 GLU A 122       19.93     52.04                                   
REMARK 500 11 LYS A 132      104.62    -55.10                                   
REMARK 500 12 ASP A   3       95.41     50.02                                   
REMARK 500 12 MET A  10      159.76    -49.94                                   
REMARK 500 12 LEU A  11      -54.12   -138.19                                   
REMARK 500 12 GLU A  14      123.64   -170.87                                   
REMARK 500 12 ASN A  15       30.14     70.07                                   
REMARK 500 12 VAL A  25      -72.37    -59.69                                   
REMARK 500 12 ASN A  26      155.90    172.83                                   
REMARK 500 12 MET A  49       77.29   -168.46                                   
REMARK 500 12 PHE A  57      -60.40   -140.06                                   
REMARK 500 12 PHE A  64      167.58     62.57                                   
REMARK 500 12 GLN A  65       85.39   -165.79                                   
REMARK 500 12 LEU A  74       58.46    -96.76                                   
REMARK 500 12 ILE A  77      -79.95    -98.18                                   
REMARK 500 12 ASP A  79       72.18     63.03                                   
REMARK 500 12 ARG A  80      139.88   -177.07                                   
REMARK 500 12 ASP A  89       50.80    -98.47                                   
REMARK 500 12 GLU A 100       36.91   -168.64                                   
REMARK 500 12 ARG A 104       86.27     48.57                                   
REMARK 500 12 GLU A 111      -97.72   -110.07                                   
REMARK 500 12 ALA A 121      132.34   -175.72                                   
REMARK 500 13 LYS A   9     -122.85   -120.11                                   
REMARK 500 13 GLU A  14      -44.15   -150.08                                   
REMARK 500 13 PRO A  38     -171.67    -68.17                                   
REMARK 500 13 MET A  49       73.39   -155.60                                   
REMARK 500 13 LEU A  74       47.53   -105.53                                   
REMARK 500 13 ILE A  77      -86.55   -105.40                                   
REMARK 500 13 LYS A  81      105.79     25.43                                   
REMARK 500 13 LYS A  92      134.52    173.36                                   
REMARK 500 13 TRP A 109      140.96    179.50                                   
REMARK 500 13 GLU A 114      118.52   -163.46                                   
REMARK 500 13 ALA A 121      123.83   -178.54                                   
REMARK 500 14 ASP A   3      113.12    -20.76                                   
REMARK 500 14 ASN A   5      109.73    -49.99                                   
REMARK 500 14 LYS A  37       69.44    171.25                                   
REMARK 500 14 ASP A  47      -17.44     86.74                                   
REMARK 500 14 HIS A  48     -166.47   -114.36                                   
REMARK 500 14 MET A  49      133.67   -171.57                                   
REMARK 500 14 ASP A  79       93.92     85.76                                   
REMARK 500 14 ARG A  80      -55.27   -123.31                                   
REMARK 500 14 LYS A  81      111.11     71.93                                   
REMARK 500 14 LYS A  98       69.91   -107.80                                   
REMARK 500 14 GLU A 100     -169.52     53.47                                   
REMARK 500 14 LYS A 101      179.15     59.64                                   
REMARK 500 14 ASP A 113      -33.38    159.38                                   
REMARK 500 14 ALA A 121      138.55    175.78                                   
REMARK 500 15 GLU A  14      -45.74   -136.28                                   
REMARK 500 15 ASN A  15       39.41   -145.75                                   
REMARK 500 15 ASN A  26      158.00    -49.59                                   
REMARK 500 15 LEU A  36      -75.76    -91.74                                   
REMARK 500 15 LYS A  37       72.29     49.07                                   
REMARK 500 15 MET A  49       79.92   -150.89                                   
REMARK 500 15 SER A  55      -46.68   -139.75                                   
REMARK 500 15 THR A  56       -7.75    117.34                                   
REMARK 500 15 ASP A  73       94.22    -64.69                                   
REMARK 500 15 ILE A  77      -60.65   -108.09                                   
REMARK 500 15 ASP A  79       38.96     71.45                                   
REMARK 500 15 ASP A  91      -11.90    106.50                                   
REMARK 500 15 LYS A 101     -165.24   -113.43                                   
REMARK 500 15 GLU A 111     -137.48   -120.63                                   
REMARK 500 15 ALA A 121      130.92   -179.34                                   
REMARK 500 16 LEU A  11      -53.21   -152.69                                   
REMARK 500 16 ASP A  24       71.79     45.37                                   
REMARK 500 16 LEU A  35       42.65   -104.02                                   
REMARK 500 16 LEU A  36      -72.16   -131.53                                   
REMARK 500 16 LYS A  37       70.86     51.90                                   
REMARK 500 16 MET A  49       78.59   -150.03                                   
REMARK 500 16 ASP A  63       46.90   -140.07                                   
REMARK 500 16 PHE A  64      105.81    -45.70                                   
REMARK 500 16 LEU A  74       58.54    -96.33                                   
REMARK 500 16 ASP A  78       11.66   -143.96                                   
REMARK 500 16 ASP A  79       72.99     55.74                                   
REMARK 500 16 TRP A  88       94.03     70.93                                   
REMARK 500 16 ASP A  91      -47.57    136.20                                   
REMARK 500 16 GLU A 100       37.09   -167.02                                   
REMARK 500 16 GLU A 102      -71.55    -74.80                                   
REMARK 500 16 ARG A 104       71.97    -10.83                                   
REMARK 500 16 ALA A 121      117.99   -174.80                                   
REMARK 500 16 VAL A 129       79.80   -101.88                                   
REMARK 500 16 VAL A 133      -61.10    -98.61                                   
REMARK 500 17 ASP A   3      126.90    168.19                                   
REMARK 500 17 LEU A  11      -57.16   -146.03                                   
REMARK 500 17 ASP A  45       67.15   -154.62                                   
REMARK 500 17 ILE A  50      102.89    -52.67                                   
REMARK 500 17 ASP A  63       54.92   -113.73                                   
REMARK 500 17 PHE A  64      137.16    -35.52                                   
REMARK 500 17 GLU A  69      152.43    -49.15                                   
REMARK 500 17 LEU A  74       57.89   -106.11                                   
REMARK 500 17 ILE A  77      -65.39   -127.05                                   
REMARK 500 17 ASP A  78       12.28   -142.47                                   
REMARK 500 17 ASP A  79       97.12     43.00                                   
REMARK 500 17 LYS A  81      101.87     61.20                                   
REMARK 500 17 GLU A 102      136.40     70.78                                   
REMARK 500 17 GLU A 111       47.98   -106.64                                   
REMARK 500 17 ASP A 113       36.32     70.41                                   
REMARK 500 17 GLU A 114      102.20   -160.18                                   
REMARK 500 17 ALA A 121      140.10    173.41                                   
REMARK 500 18 ASN A   5       88.83    -51.24                                   
REMARK 500 18 MET A  10     -178.47    -49.82                                   
REMARK 500 18 LEU A  11      -61.78   -158.17                                   
REMARK 500 18 SER A  12      148.06   -179.09                                   
REMARK 500 18 ASP A  24       75.54     51.90                                   
REMARK 500 18 LEU A  36      -74.42    -82.85                                   
REMARK 500 18 LYS A  37       71.18     53.37                                   
REMARK 500 18 LEU A  54       76.71   -111.26                                   
REMARK 500 18 PHE A  57      -68.21   -141.66                                   
REMARK 500 18 ASN A  59      106.59    -54.07                                   
REMARK 500 18 GLU A  69      132.68     86.99                                   
REMARK 500 18 LEU A  74       53.22    -96.01                                   
REMARK 500 18 ILE A  77      -70.43   -117.96                                   
REMARK 500 18 ASP A  78       27.41   -150.03                                   
REMARK 500 18 ASP A  79       92.87     35.07                                   
REMARK 500 18 ASP A  89      -66.85     -2.06                                   
REMARK 500 18 LYS A  98       51.54    -93.61                                   
REMARK 500 18 LYS A 101      136.70    105.88                                   
REMARK 500 18 ARG A 104      130.50    -34.53                                   
REMARK 500 18 TRP A 106      177.80    -59.58                                   
REMARK 500 18 THR A 107      129.21    179.92                                   
REMARK 500 18 ALA A 121      103.09   -166.57                                   
REMARK 500 19 VAL A   2      -31.77   -135.85                                   
REMARK 500 19 VAL A  25      -72.12    -50.46                                   
REMARK 500 19 ASN A  26      148.87    174.19                                   
REMARK 500 19 PRO A  38      179.17    -56.58                                   
REMARK 500 19 ASP A  45       74.81   -167.01                                   
REMARK 500 19 ARG A  58      121.74    172.81                                   
REMARK 500 19 TYR A  60     -138.08   -100.31                                   
REMARK 500 19 ILE A  61      139.18    127.12                                   
REMARK 500 19 THR A  75      107.89    -40.40                                   
REMARK 500 19 ASP A  79       73.09     50.35                                   
REMARK 500 19 ASP A  89       46.63   -154.95                                   
REMARK 500 19 LYS A 101      176.72     87.74                                   
REMARK 500 19 ARG A 104       97.30     15.91                                   
REMARK 500 20 ASN A  26      158.24    -48.85                                   
REMARK 500 20 THR A  56       -7.93     92.94                                   
REMARK 500 20 ARG A  58      -26.57    173.89                                   
REMARK 500 20 ASN A  59      153.48     77.56                                   
REMARK 500 20 TYR A  60      101.19   -170.16                                   
REMARK 500 20 MET A  62      -69.17   -147.21                                   
REMARK 500 20 ASP A  63      104.31     76.71                                   
REMARK 500 20 GLU A  72       51.81   -118.60                                   
REMARK 500 20 ILE A  77      -70.16   -117.84                                   
REMARK 500 20 ASP A  79       85.49     41.20                                   
REMARK 500 20 LYS A  92       92.79   -162.58                                   
REMARK 500 20 LYS A  98      -38.28    -36.80                                   
REMARK 500 20 GLU A 100      -46.39   -174.97                                   
REMARK 500 20 TRP A 109      -63.85   -163.81                                   
REMARK 500 20 ILE A 110      106.93     70.81                                   
REMARK 500 20 ASP A 113       35.16     75.61                                   
REMARK 500 20 GLU A 114      126.52    154.94                                   
REMARK 500 20 ALA A 121      122.22    173.85                                   
REMARK 500 21 ASN A  15       41.19   -152.19                                   
REMARK 500 21 ASN A  26      161.18    -46.58                                   
REMARK 500 21 LYS A  37       86.06   -153.77                                   
REMARK 500 21 GLU A  41       89.50   -151.64                                   
REMARK 500 21 ASP A  45      -71.91   -110.48                                   
REMARK 500 21 ASP A  47       17.59     82.21                                   
REMARK 500 21 ARG A  58      156.07    175.70                                   
REMARK 500 21 MET A  62      129.30    177.46                                   
REMARK 500 21 LEU A  74       56.23    -94.05                                   
REMARK 500 21 ASP A  79       79.21     80.25                                   
REMARK 500 21 GLU A 100      -41.85    175.02                                   
REMARK 500 22 LYS A   9      157.53    173.93                                   
REMARK 500 22 MET A  10       86.67     65.98                                   
REMARK 500 22 SER A  12       94.93    -48.74                                   
REMARK 500 22 ASN A  26      134.67    -34.45                                   
REMARK 500 22 PRO A  38     -168.13   -112.25                                   
REMARK 500 22 HIS A  48      129.11   -170.59                                   
REMARK 500 22 LEU A  54       64.38   -112.23                                   
REMARK 500 22 ARG A  58      -32.27    170.67                                   
REMARK 500 22 ASN A  59       99.88     71.63                                   
REMARK 500 22 PHE A  64      110.33    -27.34                                   
REMARK 500 22 GLU A  69      106.17     78.52                                   
REMARK 500 22 LEU A  74       61.47   -102.31                                   
REMARK 500 22 ASP A  79       75.75     45.08                                   
REMARK 500 22 GLU A 100      -45.11   -161.03                                   
REMARK 500 22 LYS A 101      -98.68    -67.50                                   
REMARK 500 22 ALA A 121      116.49    179.82                                   
REMARK 500 23 ASN A  26      154.37    -47.42                                   
REMARK 500 23 THR A  56      -62.48     79.07                                   
REMARK 500 23 VAL A  66      107.19    -40.42                                   
REMARK 500 23 LEU A  74       54.55   -102.28                                   
REMARK 500 23 ILE A  77      -66.45   -133.93                                   
REMARK 500 23 ASP A  78       53.69   -165.16                                   
REMARK 500 23 ASP A  79       75.08     11.91                                   
REMARK 500 23 LYS A  98     -174.59    -67.77                                   
REMARK 500 23 LYS A 101     -144.55   -131.47                                   
REMARK 500 23 ARG A 104       89.91     27.02                                   
REMARK 500 23 TRP A 109       99.12   -166.05                                   
REMARK 500 23 LEU A 115       47.92   -102.98                                   
REMARK 500 23 HIS A 116      124.41    -23.55                                   
REMARK 500 24 VAL A   2       82.99     31.00                                   
REMARK 500 24 LEU A  11      -58.87   -142.29                                   
REMARK 500 24 GLU A  14      106.16   -175.83                                   
REMARK 500 24 THR A  53       83.28   -163.67                                   
REMARK 500 24 PHE A  57      -39.46    173.27                                   
REMARK 500 24 ARG A  58       90.62   -164.72                                   
REMARK 500 24 TYR A  60      -74.29    -91.94                                   
REMARK 500 24 ILE A  61      161.84     55.82                                   
REMARK 500 24 MET A  62      136.57    176.37                                   
REMARK 500 24 LEU A  74       55.87   -109.45                                   
REMARK 500 24 ASP A  78       29.01   -166.20                                   
REMARK 500 24 ASP A  79       86.52     22.91                                   
REMARK 500 24 ARG A  80       52.93   -140.68                                   
REMARK 500 24 LYS A  98       44.05   -107.57                                   
REMARK 500 24 THR A 107      118.90   -163.91                                   
REMARK 500 24 TRP A 109      -53.01   -148.08                                   
REMARK 500 24 ILE A 110      145.69     73.91                                   
REMARK 500 24 GLU A 111       80.07   -155.26                                   
REMARK 500 24 ALA A 121      114.72    179.27                                   
REMARK 500 24 LYS A 131       86.46   -155.12                                   
REMARK 500 24 LYS A 132     -147.37    -68.62                                   
REMARK 500 24 VAL A 133      -49.08   -150.09                                   
REMARK 500 25 VAL A   2      -50.72   -148.52                                   
REMARK 500 25 ASP A   3      118.17    -29.97                                   
REMARK 500 25 GLU A  14      109.58   -163.39                                   
REMARK 500 25 GLN A  44      103.77    -51.87                                   
REMARK 500 25 ASP A  45       74.31   -115.61                                   
REMARK 500 25 THR A  53       86.20   -159.87                                   
REMARK 500 25 TYR A  60      -72.47   -133.33                                   
REMARK 500 25 ILE A  61      143.24     63.60                                   
REMARK 500 25 MET A  62      -52.60   -129.11                                   
REMARK 500 25 ASP A  63       61.56     65.13                                   
REMARK 500 25 LEU A  74       46.37   -103.21                                   
REMARK 500 25 ILE A  77      -63.49   -105.01                                   
REMARK 500 25 ASP A  79       82.26     55.72                                   
REMARK 500 25 LYS A  81      123.66   -171.74                                   
REMARK 500 25 LYS A  98       74.83   -115.32                                   
REMARK 500 25 LYS A 101      169.67     62.11                                   
REMARK 500 25 GLU A 102      108.31     80.64                                   
REMARK 500 25 ARG A 104      170.52     52.00                                   
REMARK 500 25 TRP A 109       87.87   -152.82                                   
REMARK 500 25 GLU A 111       78.33   -152.86                                   
REMARK 500 25 GLU A 122       28.56     47.15                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650 HELIX                                                                
REMARK 650 DETERMINATION METHOD: AUTHOR                                         
REMARK 700                                                                      
REMARK 700 SHEET                                                                
REMARK 700 DETERMINATION METHOD: AUTHOR                                         
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE RTL A 135                 
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1MX7   RELATED DB: PDB                                   
REMARK 900 SAME PROTEIN, APO STRUCTURE                                          
DBREF  1MX8 A    1   134  UNP    P02696   RET1_RAT         2    135             
SEQRES   1 A  134  PRO VAL ASP PHE ASN GLY TYR TRP LYS MET LEU SER ASN          
SEQRES   2 A  134  GLU ASN PHE GLU GLU TYR LEU ARG ALA LEU ASP VAL ASN          
SEQRES   3 A  134  VAL ALA LEU ARG LYS ILE ALA ASN LEU LEU LYS PRO ASP          
SEQRES   4 A  134  LYS GLU ILE VAL GLN ASP GLY ASP HIS MET ILE ILE ARG          
SEQRES   5 A  134  THR LEU SER THR PHE ARG ASN TYR ILE MET ASP PHE GLN          
SEQRES   6 A  134  VAL GLY LYS GLU PHE GLU GLU ASP LEU THR GLY ILE ASP          
SEQRES   7 A  134  ASP ARG LYS CYS MET THR THR VAL SER TRP ASP GLY ASP          
SEQRES   8 A  134  LYS LEU GLN CYS VAL GLN LYS GLY GLU LYS GLU GLY ARG          
SEQRES   9 A  134  GLY TRP THR GLN TRP ILE GLU GLY ASP GLU LEU HIS LEU          
SEQRES  10 A  134  GLU MET ARG ALA GLU GLY VAL THR CYS LYS GLN VAL PHE          
SEQRES  11 A  134  LYS LYS VAL HIS                                              
HET    RTL  A 135      51                                                       
HETNAM     RTL RETINOL                                                          
FORMUL   2  RTL    C20 H30 O                                                    
HELIX    1  A1 PHE A   16  ALA A   22  1                                   7    
HELIX    2  A2 VAL A   27  ASN A   34  1                                   8    
SHEET    1  B1 4 TYR A   7  GLU A  14  0                                        
SHEET    2  B1 4 LEU A  35  ASP A  45 -1  N  LYS A  40   O  TRP A   8           
SHEET    3  B1 4 HIS A  48  SER A  55 -1  O  ILE A  50   N  VAL A  43           
SHEET    4  B1 4 ASN A  59  GLN A  65 -1  N  PHE A  64   O  MET A  49           
SHEET    1  B2 6 LYS A  68  ASP A  73  0                                        
SHEET    2  B2 6 MET A  83  ASP A  89 -1  N  THR A  84   O  PHE A  70           
SHEET    3  B2 6 LYS A  92  GLN A  97 -1  N  GLN A  94   O  SER A  87           
SHEET    4  B2 6 ARG A 104  GLU A 111 -1  N  GLN A 108   O  LEU A  93           
SHEET    5  B2 6 GLU A 114  ARG A 120 -1  N  GLU A 118   O  THR A 107           
SHEET    6  B2 6 VAL A 124  LYS A 131 -1  N  GLN A 128   O  LEU A 117           
SITE     1 AC1  4 LEU A  29  MET A  62  ILE A  77  TRP A 106                    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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