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Database: PDB
Entry: 1O78
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HEADER    CARRIER PROTEIN                         24-OCT-02   1O78              
TITLE     BIOTIN CARBOXYL CARRIER DOMAIN OF TRANSCARBOXYLASE (1.3S)             
TITLE    2 [10-48] DELETION MUTANT                                              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: BIOTIN CARBOXYL CARRIER PROTEIN OF                         
COMPND   3  METHYLMALONYL-COA CARBOXYL-TRANSFERASE;                             
COMPND   4 CHAIN: A;                                                            
COMPND   5 SYNONYM: TRANSCARBOXYLASE, 1.3S SUBUNIT;                             
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES;                                                       
COMPND   8 OTHER_DETAILS: RESIDUES 10-48 DELETION MUTANT                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: PROPIONIBACTERIUM FREUDENREICHII                
SOURCE   3  SUBSP. SHERMANII;                                                   
SOURCE   4 ORGANISM_TAXID: 1752;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI;                                 
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 562;                                        
SOURCE   7 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PTAC1.3DELTA10-48, PCY216                 
KEYWDS    CARRIER PROTEIN, TRANSCARBOXYLASE, CARBOXYL CARRIER,                  
KEYWDS   2 TRANSFERASE                                                          
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    M.M.JANK,J.D.SADOWSKY,C.PEIKERT,S.BERGER                              
REVDAT   2   24-FEB-09 1O78    1       VERSN                                    
REVDAT   1   21-NOV-02 1O78    0                                                
JRNL        AUTH   M.M.JANK,J.D.SADOWSKY,C.PEIKERT,S.BERGER                     
JRNL        TITL   NMR STUDIES ON THE SOLUTION STRUCTURE OF A                   
JRNL        TITL 2 DELETION MUTANT OF THE TRANSCARBOXYLASE BIOTIN               
JRNL        TITL 3 CARRIER SUBUNIT                                              
JRNL        REF    INT.J.BIOL.MACROMOL.          V.  30   233 2002              
JRNL        REFN                   ISSN 0141-8130                               
JRNL        PMID   12297230                                                     
JRNL        DOI    10.1016/S0141-8130(02)00033-8                                
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   D.V.REDDY,B.C.SHENOY,P.R.CAREY,F.D.SONNICHSEN                
REMARK   1  TITL   HIGH RESOLUTION SOLUTION STRUCTURE OF THE 1.3S               
REMARK   1  TITL 2 SUBUNIT OF TRANSCARBOXYLASE FROM PROPIONIBACTERIUM           
REMARK   1  TITL 3 SHERMANII(DAGGER)                                            
REMARK   1  REF    BIOCHEMISTRY                  V.  39  2509 2000              
REMARK   1  REFN                   ISSN 0006-2960                               
REMARK   1  PMID   10704200                                                     
REMARK   1  DOI    10.1021/BI9925367                                            
REMARK   1 REFERENCE 2                                                          
REMARK   1  AUTH   M.M.JANK,S.BOKORNY,K.-H.ROEHM,S.BERGER                       
REMARK   1  TITL   EXPRESSION AND BIOTINYLATION OF A MUTANT OF THE              
REMARK   1  TITL 2 TRANSCARBOXYLASE CARRIER PROTEIN FROM PROPIONI               
REMARK   1  TITL 3 SHERMANII                                                    
REMARK   1  REF    PROTEIN EXPR.PURIF.           V.  17   123 1999              
REMARK   1  REFN                   ISSN 1046-5928                               
REMARK   1  PMID   10497077                                                     
REMARK   1  DOI    10.1006/PREP.1999.1097                                       
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.1                                           
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: DGSA, REFINEMENT WITH NOES AND            
REMARK   3  HYDROGEN-BOND CONSTRAINTS                                           
REMARK   4                                                                      
REMARK   4 1O78 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 25-OCT-02.                  
REMARK 100 THE PDBE ID CODE IS EBI-11596.                                       
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 300                                
REMARK 210  PH                             : 4.5                                
REMARK 210  IONIC STRENGTH                 : 2 MM AMMONIUM ACETATE              
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : 2.3 MM PROTEIN, N15/C13            
REMARK 210                                   LABELED TC 1.3S [10-48],           
REMARK 210                                   BIOTIN (UNLABELED)                 
REMARK 210                                   COVALENTLY ATTACHED TO LYS 89      
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 15N-HSQC-NOESY, HNCA,              
REMARK 210                                   HNCO, CBCANH, CBCA(CO)NH,          
REMARK 210                                   HN(CA)CO, HCACO,HN-HSQC-TOCSY,     
REMARK 210                                   HBHA(CBCACO)NH,(H)N(CA)NNH,        
REMARK 210                                   HCCH-TOCSY,2D 15N-1H HSQC          
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600                                
REMARK 210  SPECTROMETER MODEL             : DRX600                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : X-PLOR, PROCHECK 3.5.3,            
REMARK 210                                   AQUA 2.0                           
REMARK 210   METHOD USED                   : DISTANCE GEOMETRY/                 
REMARK 210                                   SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 600                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : LEAST RESTRAINT VIOLATION          
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 3                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE          
REMARK 210 NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN. BIOTIN WAS             
REMARK 210 UNLABELED AND NOT INCLUDED IN THE STRUCTURE CALCULATION.             
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 400                                                                      
REMARK 400 COMPOUND                                                             
REMARK 400  BIOTINYL 1.3S SUBUNIT (CHAIN A) SERVES AS A CARBOXYL CARRIER        
REMARK 400  BETWEEN THE SUBSTRATE BINDING SITES ON THE 12S AND 5S SUBUNITS.     
REMARK 400  TRANSCARBOXYLASE IS COMPRISED OF THREE SUBUNITS: 1.3S,              
REMARK 400  5S, AND 12S.                                                        
REMARK 400                                                                      
REMARK 400  RESIDUES 10-48 DELETION MUTANT, CHAIN A                             
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 THR A   6      159.55     59.06                                   
REMARK 500  1 LEU A  22      -49.13   -151.84                                   
REMARK 500  1 LYS A  28      133.81   -174.00                                   
REMARK 500  1 THR A  42       95.69    -52.10                                   
REMARK 500  1 LEU A  44     -160.01   -167.11                                   
REMARK 500  1 GLU A  47      100.14   -161.79                                   
REMARK 500  1 MET A  49       49.57   -149.88                                   
REMARK 500  1 MET A  51       27.32   -152.33                                   
REMARK 500  1 GLU A  52      -81.47   -143.06                                   
REMARK 500  1 THR A  53     -152.44     51.92                                   
REMARK 500  1 GLU A  54      109.77    -54.25                                   
REMARK 500  1 LYS A  65      137.47    179.17                                   
REMARK 500  1 VAL A  74       56.58     36.84                                   
REMARK 500  2 LYS A   4     -178.13     53.81                                   
REMARK 500  2 THR A   6      -70.25   -127.66                                   
REMARK 500  2 LEU A  22       59.09   -171.54                                   
REMARK 500  2 THR A  42      104.59    -59.05                                   
REMARK 500  2 LYS A  65      117.49    178.44                                   
REMARK 500  2 ARG A  71      -45.49    177.83                                   
REMARK 500  2 VAL A  74      164.41     56.44                                   
REMARK 500  2 LEU A  80      -65.90   -135.50                                   
REMARK 500  3 LYS A   2       59.67    -98.33                                   
REMARK 500  3 LEU A   3      -50.45   -162.25                                   
REMARK 500  3 THR A   6       31.20   -159.54                                   
REMARK 500  3 LYS A  28      126.71   -172.69                                   
REMARK 500  3 LYS A  32       46.01   -145.35                                   
REMARK 500  3 THR A  42       94.63    -54.42                                   
REMARK 500  3 GLU A  47     -176.38     52.82                                   
REMARK 500  3 ALA A  48      -62.60   -163.30                                   
REMARK 500  3 MET A  51      117.90   -175.79                                   
REMARK 500  3 GLU A  52       94.99    -53.12                                   
REMARK 500  3 ILE A  55       91.19    -68.85                                   
REMARK 500  3 LYS A  65       89.27    179.24                                   
REMARK 500  4 LYS A   4      -54.33   -132.18                                   
REMARK 500  4 VAL A   5      137.72     61.14                                   
REMARK 500  4 THR A   6       37.36    -99.14                                   
REMARK 500  4 LYS A  12      177.80     54.39                                   
REMARK 500  4 THR A  42      102.73    -55.68                                   
REMARK 500  4 ALA A  48     -157.92   -125.07                                   
REMARK 500  4 MET A  51      105.30     57.30                                   
REMARK 500  4 GLU A  52       74.31     57.16                                   
REMARK 500  4 LYS A  65      131.15   -179.09                                   
REMARK 500  4 LEU A  67      -58.45   -125.12                                   
REMARK 500  4 GLU A  70       80.29    -63.79                                   
REMARK 500  4 ARG A  71      -47.05   -176.88                                   
REMARK 500  4 VAL A  74       42.74     36.60                                   
REMARK 500  4 LEU A  80      -79.96    -55.73                                   
REMARK 500  5 LYS A  12      -88.42     55.24                                   
REMARK 500  5 LEU A  22       33.20   -142.18                                   
REMARK 500  5 THR A  25     -146.83   -110.23                                   
REMARK 500  5 VAL A  31     -158.03    -98.98                                   
REMARK 500  5 MET A  49      -83.29     59.04                                   
REMARK 500  5 THR A  53      -74.74     61.00                                   
REMARK 500  5 GLU A  54       90.77    -56.08                                   
REMARK 500  5 LYS A  65      138.08    179.69                                   
REMARK 500  5 LEU A  67      -67.44   -126.36                                   
REMARK 500  5 ALA A  73      142.82     61.82                                   
REMARK 500  5 VAL A  74       89.93     34.53                                   
REMARK 500  5 ILE A  81      143.37   -175.67                                   
REMARK 500  6 THR A   6       30.53     37.99                                   
REMARK 500  6 VAL A  26      100.59    -50.28                                   
REMARK 500  6 THR A  42      101.29    -57.19                                   
REMARK 500  6 ALA A  48      -80.34   -129.36                                   
REMARK 500  6 GLU A  52      109.11    -57.59                                   
REMARK 500  6 LYS A  65      156.61    179.36                                   
REMARK 500  6 VAL A  68      153.82    -46.70                                   
REMARK 500  6 GLU A  70      143.45     61.27                                   
REMARK 500  6 ASP A  72       42.62   -154.53                                   
REMARK 500  7 LEU A   3       87.00     51.87                                   
REMARK 500  7 VAL A   7      160.90     57.75                                   
REMARK 500  7 VAL A  26      168.17    -47.38                                   
REMARK 500  7 SER A  27      -42.19   -149.69                                   
REMARK 500  7 LYS A  28      119.42   -160.24                                   
REMARK 500  7 LYS A  32     -162.58   -121.38                                   
REMARK 500  7 ALA A  48     -157.24   -166.64                                   
REMARK 500  7 MET A  51       19.57     56.33                                   
REMARK 500  7 GLU A  52       96.45    179.49                                   
REMARK 500  7 LYS A  65      139.56   -177.86                                   
REMARK 500  8 LEU A   3       54.06   -160.44                                   
REMARK 500  8 VAL A   5      170.13    -54.07                                   
REMARK 500  8 THR A   6       99.95     53.99                                   
REMARK 500  8 VAL A   7       75.38   -119.70                                   
REMARK 500  8 GLU A  17     -175.46    -57.23                                   
REMARK 500  8 LYS A  28      139.94   -172.84                                   
REMARK 500  8 MET A  51       75.52     54.63                                   
REMARK 500  8 THR A  53      -83.80     56.81                                   
REMARK 500  8 GLU A  54       97.60    -58.46                                   
REMARK 500  8 THR A  59     -159.41   -165.69                                   
REMARK 500  8 LYS A  65      174.71    179.99                                   
REMARK 500  8 ASP A  72       67.55   -112.95                                   
REMARK 500  8 ALA A  73      173.30    -59.35                                   
REMARK 500  8 VAL A  74       86.46     53.55                                   
REMARK 500  8 LEU A  80      -68.57    -96.57                                   
REMARK 500  9 LYS A   2      -72.62     62.94                                   
REMARK 500  9 LYS A  12      176.80     53.73                                   
REMARK 500  9 LYS A  32       39.17   -155.93                                   
REMARK 500  9 THR A  36      178.22    -57.61                                   
REMARK 500  9 GLU A  47      109.38   -168.13                                   
REMARK 500  9 ALA A  48     -165.32   -168.30                                   
REMARK 500  9 THR A  53     -127.61     36.50                                   
REMARK 500  9 GLU A  54      133.79    -37.69                                   
REMARK 500  9 LYS A  65      163.60    179.86                                   
REMARK 500  9 ALA A  73      117.17     60.73                                   
REMARK 500 10 LYS A   4       63.30   -106.63                                   
REMARK 500 10 THR A   6       38.40   -153.85                                   
REMARK 500 10 VAL A  31     -158.07   -101.91                                   
REMARK 500 10 GLU A  47       72.60   -154.47                                   
REMARK 500 10 ALA A  48       69.70   -114.04                                   
REMARK 500 10 MET A  49       16.64     59.00                                   
REMARK 500 10 THR A  53      -92.28     52.10                                   
REMARK 500 10 GLU A  54      105.63    -59.90                                   
REMARK 500 10 ALA A  57      175.30    -53.43                                   
REMARK 500 10 THR A  59     -167.66   -163.42                                   
REMARK 500 10 LYS A  65      157.30    178.19                                   
REMARK 500 10 LEU A  67      -70.37    -56.06                                   
REMARK 500 10 LEU A  80      -70.44    -67.90                                   
REMARK 500 11 LYS A   4      -88.92     54.91                                   
REMARK 500 11 VAL A  31     -115.47   -131.52                                   
REMARK 500 11 VAL A  37     -171.79    -67.04                                   
REMARK 500 11 THR A  42      109.80    -52.22                                   
REMARK 500 11 ALA A  48      139.68   -179.32                                   
REMARK 500 11 MET A  49       78.32     54.50                                   
REMARK 500 11 THR A  53      -64.99     65.70                                   
REMARK 500 11 GLU A  54       98.83    -56.57                                   
REMARK 500 11 LYS A  65      149.26    179.97                                   
REMARK 500 11 LEU A  67      -68.52   -138.27                                   
REMARK 500 11 VAL A  68      155.12    -48.55                                   
REMARK 500 11 GLU A  70       72.69    -68.00                                   
REMARK 500 11 ARG A  71      -37.30   -176.79                                   
REMARK 500 11 VAL A  74       48.02     36.91                                   
REMARK 500 11 LEU A  80      -68.75   -109.18                                   
REMARK 500 12 LYS A  12      -38.92   -162.56                                   
REMARK 500 12 THR A  25     -141.99    -68.32                                   
REMARK 500 12 LYS A  28      108.39    -54.95                                   
REMARK 500 12 THR A  42      105.12    -55.25                                   
REMARK 500 12 ALA A  48      -69.21   -176.42                                   
REMARK 500 12 ALA A  57      170.09    -50.77                                   
REMARK 500 12 LYS A  65      169.06    178.50                                   
REMARK 500 12 LEU A  67      -52.62   -129.88                                   
REMARK 500 12 GLU A  70      135.79     61.61                                   
REMARK 500 12 VAL A  74      139.71     60.86                                   
REMARK 500 13 LYS A   4     -155.94   -143.20                                   
REMARK 500 13 THR A   6      -40.55   -161.78                                   
REMARK 500 13 LYS A  12      -74.21    -98.09                                   
REMARK 500 13 THR A  42       94.06    -53.19                                   
REMARK 500 13 MET A  49       42.41    -96.78                                   
REMARK 500 13 GLU A  52      102.21    -56.91                                   
REMARK 500 13 LYS A  65      140.43   -178.31                                   
REMARK 500 13 GLU A  70      135.94     61.97                                   
REMARK 500 14 LYS A   4      155.11     59.94                                   
REMARK 500 14 LEU A  22      127.96     61.52                                   
REMARK 500 14 LYS A  28      122.80   -171.93                                   
REMARK 500 14 THR A  42      109.91    -56.60                                   
REMARK 500 14 LYS A  50      -56.28   -168.91                                   
REMARK 500 14 MET A  51      -73.41    174.44                                   
REMARK 500 14 GLU A  52      -92.48     54.08                                   
REMARK 500 14 THR A  53      -89.78     51.67                                   
REMARK 500 14 LYS A  69     -165.73   -129.69                                   
REMARK 500 14 LEU A  80      -70.05    -92.21                                   
REMARK 500 15 LYS A   2     -166.35     52.74                                   
REMARK 500 15 LEU A   3       51.55   -168.18                                   
REMARK 500 15 THR A   6     -153.86     44.26                                   
REMARK 500 15 LEU A  22     -166.87     53.36                                   
REMARK 500 15 LYS A  28      140.78   -175.52                                   
REMARK 500 15 LYS A  32       52.43   -145.93                                   
REMARK 500 15 LYS A  38       49.58   -142.70                                   
REMARK 500 15 GLU A  52      100.25     55.33                                   
REMARK 500 15 LYS A  65      156.06    179.83                                   
REMARK 500 15 LYS A  69     -164.87   -125.73                                   
REMARK 500 15 ALA A  73      111.12     56.33                                   
REMARK 500 15 LEU A  80      -61.01   -107.64                                   
REMARK 500 16 LYS A   4       24.51   -144.85                                   
REMARK 500 16 VAL A   7       58.30   -165.22                                   
REMARK 500 16 LYS A  12       70.93     56.36                                   
REMARK 500 16 THR A  25     -133.34   -122.99                                   
REMARK 500 16 LYS A  28       94.71    -51.93                                   
REMARK 500 16 ILE A  29       86.74    -68.09                                   
REMARK 500 16 GLU A  52       88.62     53.17                                   
REMARK 500 16 LYS A  65      148.14   -174.84                                   
REMARK 500 16 LEU A  67      -53.31   -130.97                                   
REMARK 500 16 GLU A  70      132.39     61.95                                   
REMARK 500 17 LYS A   2     -170.91    -62.27                                   
REMARK 500 17 THR A   6       82.33     52.93                                   
REMARK 500 17 LYS A  12     -160.02     54.00                                   
REMARK 500 17 ALA A  20       74.34   -113.25                                   
REMARK 500 17 ALA A  48       73.81   -167.93                                   
REMARK 500 17 LYS A  50       29.59   -160.73                                   
REMARK 500 17 MET A  51      -85.67     57.04                                   
REMARK 500 17 GLU A  52      -77.15     62.86                                   
REMARK 500 17 THR A  53      -92.92     44.77                                   
REMARK 500 17 GLU A  54      104.62    -52.14                                   
REMARK 500 17 THR A  59     -171.86   -170.62                                   
REMARK 500 17 LYS A  65      161.10    177.24                                   
REMARK 500 17 ARG A  71      -42.63     74.09                                   
REMARK 500 17 ALA A  73     -156.83   -165.12                                   
REMARK 500 17 VAL A  74      115.85     57.40                                   
REMARK 500 17 LEU A  80      -70.76    -91.51                                   
REMARK 500 18 LEU A   3      -60.13   -125.07                                   
REMARK 500 18 VAL A   7      151.11     60.05                                   
REMARK 500 18 MET A  49       90.56     51.56                                   
REMARK 500 18 MET A  51      -63.28     65.58                                   
REMARK 500 18 GLU A  52      -65.15     67.76                                   
REMARK 500 18 THR A  53     -103.46     52.45                                   
REMARK 500 18 LYS A  65      166.64    179.55                                   
REMARK 500 18 ARG A  71      -36.73   -176.92                                   
REMARK 500 18 ASP A  72      107.84    -51.96                                   
REMARK 500 18 VAL A  74      113.17     57.43                                   
REMARK 500 19 VAL A   7      -77.09   -117.10                                   
REMARK 500 19 LYS A  12      164.92     57.38                                   
REMARK 500 19 SER A  27      -39.52   -134.98                                   
REMARK 500 19 THR A  42      100.93    -53.57                                   
REMARK 500 19 ALA A  48     -156.45   -126.21                                   
REMARK 500 19 MET A  49       39.82    -92.73                                   
REMARK 500 19 LYS A  50      -47.03   -153.04                                   
REMARK 500 19 MET A  51     -173.93   -170.27                                   
REMARK 500 19 LYS A  65      107.14    178.19                                   
REMARK 500 19 GLU A  70       68.10    -68.99                                   
REMARK 500 19 ARG A  71      -42.79   -173.62                                   
REMARK 500 19 VAL A  74       68.82     36.22                                   
REMARK 500 19 LEU A  80      -71.52   -102.15                                   
REMARK 500 20 LEU A   3       93.87    -66.51                                   
REMARK 500 20 LEU A  22      -53.08   -155.26                                   
REMARK 500 20 LYS A  32      -45.91   -165.78                                   
REMARK 500 20 ALA A  48     -160.24   -128.02                                   
REMARK 500 20 GLU A  52       73.37   -154.10                                   
REMARK 500 20 THR A  53      -59.96     66.84                                   
REMARK 500 20 ALA A  57      166.21    -47.84                                   
REMARK 500 20 LYS A  65      164.65    176.87                                   
REMARK 500 20 GLU A  70       76.50    -65.65                                   
REMARK 500 20 ARG A  71      -51.30    179.90                                   
REMARK 500 20 VAL A  74       49.60     36.73                                   
REMARK 500 20 LEU A  80      -58.02   -131.84                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A  71         0.19    SIDE CHAIN                              
REMARK 500  2 ARG A  71         0.31    SIDE CHAIN                              
REMARK 500  3 ARG A  71         0.20    SIDE CHAIN                              
REMARK 500  4 ARG A  71         0.24    SIDE CHAIN                              
REMARK 500  5 ARG A  71         0.25    SIDE CHAIN                              
REMARK 500  6 ARG A  71         0.27    SIDE CHAIN                              
REMARK 500  7 ARG A  71         0.24    SIDE CHAIN                              
REMARK 500  8 ARG A  71         0.31    SIDE CHAIN                              
REMARK 500  9 ARG A  71         0.24    SIDE CHAIN                              
REMARK 500 10 ARG A  71         0.21    SIDE CHAIN                              
REMARK 500 11 ARG A  71         0.21    SIDE CHAIN                              
REMARK 500 12 ARG A  71         0.25    SIDE CHAIN                              
REMARK 500 13 ARG A  71         0.20    SIDE CHAIN                              
REMARK 500 14 ARG A  71         0.30    SIDE CHAIN                              
REMARK 500 15 ARG A  71         0.20    SIDE CHAIN                              
REMARK 500 16 ARG A  71         0.19    SIDE CHAIN                              
REMARK 500 17 ARG A  71         0.21    SIDE CHAIN                              
REMARK 500 18 ARG A  71         0.17    SIDE CHAIN                              
REMARK 500 19 ARG A  71         0.24    SIDE CHAIN                              
REMARK 500 20 ARG A  71         0.28    SIDE CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1DCZ   RELATED DB: PDB                                   
REMARK 900  BIOTIN CARBOXYL CARRIER DOMAIN OF                                   
REMARK 900  TRANSCARBOXYLASE (TC 1.3S)                                          
REMARK 900 RELATED ID: 1DD2   RELATED DB: PDB                                   
REMARK 900  BIOTIN CARBOXYL CARRIER DOMAIN OF                                   
REMARK 900  TRANSCARBOXYLASE (TC 1.3S)                                          
REMARK 999                                                                      
REMARK 999 SEQUENCE                                                             
REMARK 999  RESIDUES 10-48 DELETION MUTANT                                      
DBREF  1O78 A    1     9  UNP    P02904   BCCP_PROFR       1      9             
DBREF  1O78 A   10    84  UNP    P02904   BCCP_PROFR      49    123             
SEQRES   1 A   84  MET LYS LEU LYS VAL THR VAL ASN GLY ALA GLY LYS ALA          
SEQRES   2 A   84  GLY GLU GLY GLU ILE PRO ALA PRO LEU ALA GLY THR VAL          
SEQRES   3 A   84  SER LYS ILE LEU VAL LYS GLU GLY ASP THR VAL LYS ALA          
SEQRES   4 A   84  GLY GLN THR VAL LEU VAL LEU GLU ALA MET LYS MET GLU          
SEQRES   5 A   84  THR GLU ILE ASN ALA PRO THR ASP GLY LYS VAL GLU LYS          
SEQRES   6 A   84  VAL LEU VAL LYS GLU ARG ASP ALA VAL GLN GLY GLY GLN          
SEQRES   7 A   84  GLY LEU ILE LYS ILE GLY                                      
SHEET    1  AA 3 LYS A  28  ILE A  29  0                                        
SHEET    2  AA 3 THR A  42  VAL A  45 -1  N  VAL A  45   O  LYS A  28           
SHEET    3  AA 3 ILE A  55  ASN A  56 -1  O  ILE A  55   N  VAL A  43           
SHEET    1  AB 3 THR A  36  VAL A  37  0                                        
SHEET    2  AB 3 GLY A  61  VAL A  66 -1  O  GLY A  61   N  VAL A  37           
SHEET    3  AB 3 ILE A  81  ILE A  83 -1  O  LYS A  82   N  GLU A  64           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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