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Database: PDB
Entry: 1P9J
LinkDB: 1P9J
Original site: 1P9J 
HEADER    HORMONE/GROWTH FACTOR                   12-MAY-03   1P9J              
TITLE     SOLUTION STRUCTURE AND DYNAMICS OF THE EGF/TGF-ALPHA                  
TITLE    2 CHIMERA T1E                                                          
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CHIMERA OF EPIDERMAL GROWTH FACTOR(EGF) AND                
COMPND   3 TRANSFORMING GROWTH FACTOR ALPHA (TGF-ALPHA);                        
COMPND   4 CHAIN: A;                                                            
COMPND   5 FRAGMENT: TGF-ALPHA (RESIDUES 1-7), EGF (RESIDUES 8-54);             
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TGF-ALPHA AND EGF;                                             
SOURCE   6 EXPRESSION_SYSTEM: PICHIA PASTORIS;                                  
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 4922;                                       
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: X33                                        
KEYWDS    CHIMERA, EGF, TGF-ALPHA, ERBB1, ERBB3, HORMONE/GROWTH                 
KEYWDS   2 FACTOR COMPLEX                                                       
EXPDTA    SOLUTION NMR                                                          
NUMMDL    36                                                                    
AUTHOR    M.WINGENS,T.WALMA,H.VAN INGEN,C.STORTELERS,J.E.VAN LEEUWEN,           
AUTHOR   2 E.J.VAN ZOELEN,G.W.VUISTER                                           
REVDAT   2   24-FEB-09 1P9J    1       VERSN                                    
REVDAT   1   07-OCT-03 1P9J    0                                                
JRNL        AUTH   M.WINGENS,T.WALMA,H.VAN INGEN,C.STORTELERS,                  
JRNL        AUTH 2 J.E.VAN LEEUWEN,E.J.VAN ZOELEN,G.W.VUISTER                   
JRNL        TITL   STRUCTURAL ANALYSIS OF AN EPIDERMAL GROWTH                   
JRNL        TITL 2 FACTOR/TRANSFORMING GROWTH FACTOR-ALPHA CHIMERA              
JRNL        TITL 3 WITH UNIQUE ERBB BINDING SPECIFICITY.                        
JRNL        REF    J.BIOL.CHEM.                  V. 278 39114 2003              
JRNL        REFN                   ISSN 0021-9258                               
JRNL        PMID   12869572                                                     
JRNL        DOI    10.1074/JBC.M305603200                                       
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.851, CHARMM 22                              
REMARK   3   AUTHORS     : BRUNGER, A. (X-PLOR), MACKERELL, A.D. (CHARMM)       
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: THE STRUCTURES ARE BASED ON 660 ARE       
REMARK   3  NOE-DERIVED DISTANCE CONSTRAINTS, 98 DIHEDRAL ANGLE                 
REMARK   3  RESTRAINTS, AND 9 DISTANCE RESTRAINTS FROM HYDROGEN BONDS.          
REMARK   4                                                                      
REMARK   4 1P9J COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 15-MAY-03.                  
REMARK 100 THE RCSB ID CODE IS RCSB019177.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 6.3                                
REMARK 210  IONIC STRENGTH                 : 50                                 
REMARK 210  PRESSURE                       : 1 ATM                              
REMARK 210  SAMPLE CONTENTS                : 0.8MM T1E, 15N-LABELLED, 50MM      
REMARK 210                                   PHOSPHATE BUFFER, 95% H20, 5%      
REMARK 210                                   D20, PH 6.3, 20UG/ML PEFABLOC      
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 2D NOESY, 3D_15N-SEPARATED_        
REMARK 210                                   NOESY                              
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ, 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NMRPIPE 0203                       
REMARK 210   METHOD USED                   : DISTANCE GEOMETRY FOLLOWED BY      
REMARK 210                                   BRIEF MOLECULAR DYNAMICS RUN       
REMARK 210                                   IN WATER.                          
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 98                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 36                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500  2 ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.0 DEGREES          
REMARK 500  6 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  7 CYS A   8   CA  -  CB  -  SG  ANGL. DEV. =   7.8 DEGREES          
REMARK 500  8 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -4.1 DEGREES          
REMARK 500  8 TYR A  46   CB  -  CG  -  CD2 ANGL. DEV. =  -5.4 DEGREES          
REMARK 500  8 TYR A  46   CB  -  CG  -  CD1 ANGL. DEV. =   4.7 DEGREES          
REMARK 500  9 ARG A  43   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES          
REMARK 500 11 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -5.5 DEGREES          
REMARK 500 11 TYR A  24   CB  -  CG  -  CD1 ANGL. DEV. =   4.8 DEGREES          
REMARK 500 11 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   4.2 DEGREES          
REMARK 500 12 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -3.9 DEGREES          
REMARK 500 12 ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 12 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500 13 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 15 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -3.7 DEGREES          
REMARK 500 15 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500 17 ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500 18 ARG A  43   NE  -  CZ  -  NH2 ANGL. DEV. =  -3.5 DEGREES          
REMARK 500 19 TYR A  31   CB  -  CG  -  CD2 ANGL. DEV. =  -6.3 DEGREES          
REMARK 500 19 TYR A  31   CB  -  CG  -  CD1 ANGL. DEV. =   5.3 DEGREES          
REMARK 500 20 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -5.1 DEGREES          
REMARK 500 20 TYR A  24   CB  -  CG  -  CD1 ANGL. DEV. =   4.1 DEGREES          
REMARK 500 20 TYR A  46   CB  -  CG  -  CD2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500 22 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -6.6 DEGREES          
REMARK 500 22 TYR A  24   CB  -  CG  -  CD1 ANGL. DEV. =   5.5 DEGREES          
REMARK 500 23 ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.0 DEGREES          
REMARK 500 27 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -4.3 DEGREES          
REMARK 500 28 CYS A   8   CA  -  CB  -  SG  ANGL. DEV. =   7.8 DEGREES          
REMARK 500 29 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -4.1 DEGREES          
REMARK 500 29 TYR A  46   CB  -  CG  -  CD2 ANGL. DEV. =  -5.4 DEGREES          
REMARK 500 29 TYR A  46   CB  -  CG  -  CD1 ANGL. DEV. =   4.7 DEGREES          
REMARK 500 31 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -5.5 DEGREES          
REMARK 500 31 TYR A  24   CB  -  CG  -  CD1 ANGL. DEV. =   4.8 DEGREES          
REMARK 500 31 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   4.2 DEGREES          
REMARK 500 32 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -3.9 DEGREES          
REMARK 500 32 ARG A  43   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500 32 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   3.5 DEGREES          
REMARK 500 33 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500 35 TYR A  24   CB  -  CG  -  CD2 ANGL. DEV. =  -3.7 DEGREES          
REMARK 500 35 ARG A  47   NE  -  CZ  -  NH1 ANGL. DEV. =   3.6 DEGREES          
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASP A  13       35.33    -99.72                                   
REMARK 500  1 ASP A  19       62.96     68.61                                   
REMARK 500  2 CYS A   8       96.70    -52.00                                   
REMARK 500  2 HIS A  18       30.21     73.62                                   
REMARK 500  2 ASP A  19       80.88     77.06                                   
REMARK 500  2 ASP A  29       16.82     80.73                                   
REMARK 500  3 ASP A  19       79.75     59.74                                   
REMARK 500  3 GLU A  53       46.18    -70.28                                   
REMARK 500  4 ASP A  19       81.06     58.04                                   
REMARK 500  4 ASP A  29       31.06     81.04                                   
REMARK 500  4 GLU A  53       59.78    -91.65                                   
REMARK 500  5 SER A   3      -53.71   -130.40                                   
REMARK 500  5 CYS A   8       79.77   -114.63                                   
REMARK 500  5 ASP A  19       74.54     56.22                                   
REMARK 500  5 GLU A  53       72.15   -103.94                                   
REMARK 500  6 ASP A  19       76.86     59.47                                   
REMARK 500  6 ASP A  29        9.08     84.25                                   
REMARK 500  6 GLU A  53       63.71   -100.32                                   
REMARK 500  7 HIS A  12      -39.21   -150.14                                   
REMARK 500  7 ASP A  19       74.53     51.45                                   
REMARK 500  8 HIS A  12       72.91    -15.88                                   
REMARK 500  8 GLU A  53       64.55   -102.83                                   
REMARK 500  9 ASP A   7       97.74   -160.90                                   
REMARK 500  9 HIS A  12       93.87    -16.66                                   
REMARK 500 10 HIS A  12       34.78   -150.02                                   
REMARK 500 10 ILE A  40     -165.12   -129.55                                   
REMARK 500 10 GLU A  53       73.41   -107.32                                   
REMARK 500 11 ASP A  19       72.28     33.08                                   
REMARK 500 11 ASP A  29       51.22     71.23                                   
REMARK 500 11 ARG A  43       37.95    -94.75                                   
REMARK 500 11 CYS A  44       31.72     71.28                                   
REMARK 500 12 CYS A   8      107.50    -57.74                                   
REMARK 500 12 ASP A  29        7.39     83.49                                   
REMARK 500 12 GLU A  53       73.77   -113.15                                   
REMARK 500 14 SER A   3       55.04    -99.92                                   
REMARK 500 14 ASP A  19       80.13     73.77                                   
REMARK 500 15 SER A   3      -49.03   -130.40                                   
REMARK 500 15 HIS A  12      114.36    -20.69                                   
REMARK 500 15 GLU A  53       69.11   -103.56                                   
REMARK 500 16 PRO A   9      -64.26   -103.42                                   
REMARK 500 16 HIS A  18       45.85     78.09                                   
REMARK 500 16 GLU A  53       44.84    -93.07                                   
REMARK 500 17 ASP A  13       35.33    -99.72                                   
REMARK 500 17 ASP A  19       62.96     68.61                                   
REMARK 500 18 ASP A   7       97.74   -160.90                                   
REMARK 500 18 HIS A  12       93.87    -16.66                                   
REMARK 500 19 ASP A  19       40.19     72.04                                   
REMARK 500 19 ASP A  29       10.22     84.48                                   
REMARK 500 19 CYS A  44       70.55     54.38                                   
REMARK 500 20 SER A   3      -50.30   -120.72                                   
REMARK 500 20 ASP A  19       75.95     53.54                                   
REMARK 500 21 SER A   3      -52.51   -130.39                                   
REMARK 500 21 ASP A   7     -168.41   -122.06                                   
REMARK 500 21 CYS A   8      109.29    -50.55                                   
REMARK 500 21 GLU A  53       57.95   -114.20                                   
REMARK 500 22 SER A   3      -50.96   -120.26                                   
REMARK 500 22 PRO A   9       48.51    -84.48                                   
REMARK 500 22 ASP A  19       75.18     64.66                                   
REMARK 500 23 CYS A   8       96.70    -52.00                                   
REMARK 500 23 HIS A  18       30.21     73.62                                   
REMARK 500 23 ASP A  19       80.88     77.06                                   
REMARK 500 23 ASP A  29       16.82     80.73                                   
REMARK 500 24 ASP A  19       79.75     59.74                                   
REMARK 500 24 GLU A  53       46.18    -70.28                                   
REMARK 500 25 ASP A  19       81.06     58.04                                   
REMARK 500 25 ASP A  29       31.06     81.04                                   
REMARK 500 25 GLU A  53       59.78    -91.65                                   
REMARK 500 26 SER A   3      -53.71   -130.40                                   
REMARK 500 26 CYS A   8       79.77   -114.63                                   
REMARK 500 26 ASP A  19       74.54     56.22                                   
REMARK 500 26 GLU A  53       72.15   -103.94                                   
REMARK 500 27 ASP A  19       76.86     59.47                                   
REMARK 500 27 ASP A  29        9.08     84.25                                   
REMARK 500 27 GLU A  53       63.71   -100.32                                   
REMARK 500 28 HIS A  12      -39.21   -150.14                                   
REMARK 500 28 ASP A  19       74.53     51.45                                   
REMARK 500 29 HIS A  12       72.91    -15.88                                   
REMARK 500 29 GLU A  53       64.55   -102.83                                   
REMARK 500 30 HIS A  12       34.78   -150.02                                   
REMARK 500 30 ILE A  40     -165.12   -129.55                                   
REMARK 500 30 GLU A  53       73.41   -107.32                                   
REMARK 500 31 ASP A  19       72.28     33.08                                   
REMARK 500 31 ASP A  29       51.22     71.23                                   
REMARK 500 31 ARG A  43       37.95    -94.75                                   
REMARK 500 31 CYS A  44       31.72     71.28                                   
REMARK 500 32 CYS A   8      107.50    -57.74                                   
REMARK 500 32 ASP A  29        7.39     83.49                                   
REMARK 500 32 GLU A  53       73.77   -113.15                                   
REMARK 500 34 SER A   3       55.04    -99.92                                   
REMARK 500 34 ASP A  19       80.13     73.77                                   
REMARK 500 35 SER A   3      -49.03   -130.40                                   
REMARK 500 35 HIS A  12      114.36    -20.69                                   
REMARK 500 35 GLU A  53       69.11   -103.56                                   
REMARK 500 36 PRO A   9      -64.26   -103.42                                   
REMARK 500 36 HIS A  18       45.85     78.09                                   
REMARK 500 36 GLU A  53       44.84    -93.07                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: NON-CIS, NON-TRANS                                         
REMARK 500                                                                      
REMARK 500 THE FOLLOWING PEPTIDE BONDS DEVIATE SIGNIFICANTLY FROM BOTH          
REMARK 500 CIS AND TRANS CONFORMATION.  CIS BONDS, IF ANY, ARE LISTED           
REMARK 500 ON CISPEP RECORDS.  TRANS IS DEFINED AS 180 +/- 30 AND               
REMARK 500 CIS IS DEFINED AS 0 +/- 30 DEGREES.                                  
REMARK 500                                 MODEL     OMEGA                      
REMARK 500 SER A   11     HIS A   12          8      -148.85                    
REMARK 500 SER A   11     HIS A   12          9      -147.73                    
REMARK 500 SER A   11     HIS A   12         18      -147.73                    
REMARK 500 SER A   11     HIS A   12         29      -148.85                    
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  2 ARG A  43         0.09    SIDE_CHAIN                              
REMARK 500  5 ARG A  43         0.14    SIDE_CHAIN                              
REMARK 500  6 ARG A  43         0.10    SIDE_CHAIN                              
REMARK 500  9 ARG A  47         0.09    SIDE_CHAIN                              
REMARK 500 10 TYR A  46         0.08    SIDE_CHAIN                              
REMARK 500 11 TYR A  39         0.09    SIDE_CHAIN                              
REMARK 500 12 ARG A  43         0.15    SIDE_CHAIN                              
REMARK 500 15 ARG A  43         0.09    SIDE_CHAIN                              
REMARK 500 18 ARG A  47         0.09    SIDE_CHAIN                              
REMARK 500 22 TYR A  24         0.08    SIDE_CHAIN                              
REMARK 500 23 ARG A  43         0.09    SIDE_CHAIN                              
REMARK 500 26 ARG A  43         0.14    SIDE_CHAIN                              
REMARK 500 27 ARG A  43         0.10    SIDE_CHAIN                              
REMARK 500 30 TYR A  46         0.08    SIDE_CHAIN                              
REMARK 500 31 TYR A  39         0.09    SIDE_CHAIN                              
REMARK 500 32 ARG A  43         0.15    SIDE_CHAIN                              
REMARK 500 35 ARG A  43         0.09    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 5801   RELATED DB: BMRB                                  
REMARK 900 CHEMICAL SHIFT, J COUPLING CONSTANTS AND RELAXATION DATA             
DBREF  1P9J A    1     7  UNP    P01135   TGFA_HUMAN      40     46             
DBREF  1P9J A    8    54  UNP    P01133   EGF_HUMAN      976   1022             
SEQRES   1 A   54  VAL VAL SER HIS PHE ASN ASP CYS PRO LEU SER HIS ASP          
SEQRES   2 A   54  GLY TYR CYS LEU HIS ASP GLY VAL CYS MET TYR ILE GLU          
SEQRES   3 A   54  ALA LEU ASP LYS TYR ALA CYS ASN CYS VAL VAL GLY TYR          
SEQRES   4 A   54  ILE GLY GLU ARG CYS GLN TYR ARG ASP LEU LYS TRP TRP          
SEQRES   5 A   54  GLU LEU                                                      
SHEET    1   A 2 VAL A  21  ILE A  25  0                                        
SHEET    2   A 2 LYS A  30  ASN A  34 -1  O  LYS A  30   N  ILE A  25           
SHEET    1   B 2 TYR A  39  ILE A  40  0                                        
SHEET    2   B 2 TYR A  46  ARG A  47 -1  O  TYR A  46   N  ILE A  40           
SSBOND   1 CYS A    8    CYS A   22                          1555   1555  2.03  
SSBOND   2 CYS A   16    CYS A   33                          1555   1555  2.03  
SSBOND   3 CYS A   35    CYS A   44                          1555   1555  2.03  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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