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Database: PDB
Entry: 1PLO
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Original site: 1PLO 
HEADER    CYTOKINE RECEPTOR                       08-JUN-03   1PLO              
TITLE     TRANSFORMING GROWTH FACTOR-BETA TYPE II RECEPTOR                      
TITLE    2 EXTRACELLULAR DOMAIN                                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TGF-BETA RECEPTOR TYPE II;                                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: TGFR-2, TGF-BETA TYPE II RECEPTOR;                          
COMPND   5 EC: 2.7.1.37;                                                        
COMPND   6 ENGINEERED: YES;                                                     
COMPND   7 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: TGFBR2;                                                        
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET32A                                    
KEYWDS    THREE-FINGER TOXIN FOLD, CYTOKINE RECEPTOR                            
EXPDTA    SOLUTION NMR                                                          
NUMMDL    10                                                                    
AUTHOR    S.DEEP,K.P.WALKER III,Z.SHU,A.P.HINCK                                 
REVDAT   2   24-FEB-09 1PLO    1       VERSN                                    
REVDAT   1   23-SEP-03 1PLO    0                                                
JRNL        AUTH   S.DEEP,K.P.WALKER III,Z.SHU,A.P.HINCK                        
JRNL        TITL   SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF THE              
JRNL        TITL 2 TGF-BETA TYPE II RECEPTOR EXTRACELLULAR DOMAIN               
JRNL        REF    BIOCHEMISTRY                  V.  42 10126 2003              
JRNL        REFN                   ISSN 0006-2960                               
JRNL        PMID   12939140                                                     
JRNL        DOI    10.1021/BI034366A                                            
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : XPLOR_NIH 1.06                                       
REMARK   3   AUTHORS     : CLORE, G.M., KUSZEWSKI, J., SCHWIETERS, C.D.,        
REMARK   3                 TJANDRA, N.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: STRUCTURES ARE BASED ON A TOTAL OF        
REMARK   3  1583 CONSTRAINTS, 1168 ARE NOE DISTANCE, 138 ARE DIHEDREDRAL        
REMARK   3  ANGLE, 58 ARE 3JHNHA COUPLING CONSTANT, 219 ARE RESIDUAL            
REMARK   3  DIPOLAR COUPLINGS,                                                  
REMARK   4                                                                      
REMARK   4 1PLO COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-JUN-03.                  
REMARK 100 THE RCSB ID CODE IS RCSB019411.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 300; 300; 313                      
REMARK 210  PH                             : 5.5; 5.1; 5.5                      
REMARK 210  IONIC STRENGTH                 : 25MM SODIUM ACETATE, 25MM          
REMARK 210                                   NACL, PH 5.5; 25MM SODIUM          
REMARK 210                                   ACETATE, 25MM NACL; 25MM           
REMARK 210                                   SODIUM ACETATE, 25MM NACL          
REMARK 210  PRESSURE                       : AMBIENT; AMBIENT; AMBIENT          
REMARK 210  SAMPLE CONTENTS                : 1MM ECTBR2 U-15N, 25MM SODIUM      
REMARK 210  ACETATE, 25MM NACL, 95% H2O, 5% D2O; 1MM ECTBR2 U-15N, 13C,         
REMARK 210  25MM SODIUM ACETATE, 25MM NACL, 95% H2O, 5% D2O; 1MM ECTBR2 U-      
REMARK 210  15N, 13C, 25MM SODIUM ACETATE, 25MM NACL, 99.99% D2O; 1MM           
REMARK 210  ECTBR2 U-15N, 25MM SODIUM ACETATE, 25MM NACL, 4% (W/V) 1,2-DI-      
REMARK 210  O-HEXYL-SN-GLYCERO-3-PHOSPHOCHOLINE (6-O-PC) AND 1,2-DI-O-          
REMARK 210  TETRADECYL-SN-GLYCERO-3-PHOSPHOCHOLINE (14-O-PC) MIXED IN A         
REMARK 210  MOLAR RATIO OF 1:3, 95% H2O, 5% D2O; 1MM ECTBR2 U-15N, 13C,         
REMARK 210  25MM SODIUM ACETATE, 25MM NACL, 4% (W/V) 1,2-DI-O-HEXYL-SN-         
REMARK 210  GLYCERO-3-PHOSPHOCHOLINE (6-O-PC) AND 1,2-DI-O-TETRADECYL-SN-       
REMARK 210  GLYCERO-3-PHOSPHOCHOLINE (14-O-PC) MIXED IN A MOLAR RATIO OF        
REMARK 210  1:3, 95% H2O, 5% D2O                                                
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY, 4D_13C-       
REMARK 210                                   SEPARATED_NOESY, 3D 15N            
REMARK 210                                   EDITED, 13C-EDITED 1H-1H           
REMARK 210                                   NOESY, 3D 15N-EDITED,15N-          
REMARK 210                                   EDITED 1H-1H NOESY, 1H-15H 1-      
REMARK 210                                   BOND RESIDUAL DIPOLAR COUPLING     
REMARK 210                                   (2D IPAP-HSQC), 13C-13CO           
REMARK 210                                   RESIDUAL DIPOLAR COUPLING (3D      
REMARK 210                                   (HA)CA(CO)NH, 13CALPHA-13CO        
REMARK 210                                   RESIDUAL DIPOLAR COUPLING (3D      
REMARK 210                                   CA-COUPLED HNCO)                   
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 500 MHZ                   
REMARK 210  SPECTROMETER MODEL             : DRX, AVANCE                        
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NULL                               
REMARK 210   METHOD USED                   : SUBSTRUCTURE DISTANCE              
REMARK 210                                   GEOMETRY, FULL STRUCTURE           
REMARK 210                                   DISTANCE GEOMETRY-SIMULATED        
REMARK 210                                   ANNEALING; TORSION ANGLE           
REMARK 210                                   DYNAMICS                           
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 50                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 10                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH ACCEPTABLE         
REMARK 210                                   COVALENT GEOMETRY, STRUCTURES      
REMARK 210                                   WITH FAVORABLE NON-BOND            
REMARK 210                                   ENERGY, STRUCTURES WITH THE        
REMARK 210                                   LEAST RESTRAINT VIOLATIONS,        
REMARK 210                                   STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THIS STRUCTURE WAS DETERMINED BY REFINING                    
REMARK 210  SIMULTANEOUSLY AGAINST NOE, J-COUPLING, DIHEDRAL ANGLE, AND         
REMARK 210  RESIDUAL DIPOLAR COUPLING CONSTRAINTS                               
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 VAL A  22       34.78   -178.60                                   
REMARK 500  1 ASP A  32     -142.71     68.84                                   
REMARK 500  1 ASP A  39      -95.57    -90.70                                   
REMARK 500  1 GLN A  41     -143.10    -77.88                                   
REMARK 500  1 LYS A  42      102.46     55.32                                   
REMARK 500  1 SER A  43       84.23     43.51                                   
REMARK 500  1 CYS A  48      -42.96   -147.89                                   
REMARK 500  1 VAL A  60     -166.94   -121.78                                   
REMARK 500  1 ASP A  69      -77.58     56.55                                   
REMARK 500  1 GLU A  91        7.88   -159.96                                   
REMARK 500  1 GLU A 102      149.53     47.10                                   
REMARK 500  1 CYS A 113      114.82   -168.71                                   
REMARK 500  1 ASP A 118     -101.17    -38.27                                   
REMARK 500  1 SER A 127        3.95   -156.55                                   
REMARK 500  1 GLU A 128      -74.54     58.36                                   
REMARK 500  2 ALA A  19      174.46     52.90                                   
REMARK 500  2 ALA A  21      -37.63   -172.20                                   
REMARK 500  2 ASP A  32     -161.91     80.89                                   
REMARK 500  2 ASN A  40       95.84     32.66                                   
REMARK 500  2 GLN A  41      -86.19   -160.86                                   
REMARK 500  2 LYS A  42      -55.57   -156.18                                   
REMARK 500  2 VAL A  60     -163.01   -114.96                                   
REMARK 500  2 ASP A  69      -66.73     66.99                                   
REMARK 500  2 GLU A  70      -21.90   -140.48                                   
REMARK 500  2 PRO A  81       49.41    -82.38                                   
REMARK 500  2 ASP A  87       30.90     70.46                                   
REMARK 500  2 GLU A  91       57.10   -162.24                                   
REMARK 500  2 ASP A  92       -0.86   -148.54                                   
REMARK 500  2 MET A 100       99.29     55.11                                   
REMARK 500  2 GLU A 102      118.37     25.04                                   
REMARK 500  2 CYS A 113      146.23   -178.90                                   
REMARK 500  2 GLU A 119       78.87     53.00                                   
REMARK 500  2 GLU A 128      -72.79   -157.51                                   
REMARK 500  2 TYR A 130      -29.40   -179.63                                   
REMARK 500  2 ASN A 131       58.01   -172.29                                   
REMARK 500  2 THR A 132      -92.58     54.10                                   
REMARK 500  3 ASP A  32     -160.46     80.43                                   
REMARK 500  3 ASN A  40       95.12     34.08                                   
REMARK 500  3 GLN A  41      -84.90   -161.92                                   
REMARK 500  3 LYS A  42      -57.43   -156.85                                   
REMARK 500  3 SER A  46      148.08     60.34                                   
REMARK 500  3 ASP A  69      -68.49     64.39                                   
REMARK 500  3 GLU A  70      -36.15   -131.66                                   
REMARK 500  3 HIS A  86      -51.72     69.48                                   
REMARK 500  3 ASP A  87      -18.46   -160.28                                   
REMARK 500  3 GLU A  91       64.47   -159.92                                   
REMARK 500  3 ASP A  92        3.85   -151.12                                   
REMARK 500  3 ILE A  99       75.59    -66.94                                   
REMARK 500  3 GLU A 119      119.89     54.26                                   
REMARK 500  3 CYS A 120       20.21   -159.91                                   
REMARK 500  3 SER A 127      -65.12   -158.67                                   
REMARK 500  3 GLU A 128      -80.53     59.70                                   
REMARK 500  3 GLU A 129       10.16   -142.14                                   
REMARK 500  3 ASN A 134       80.06     54.24                                   
REMARK 500  4 ASP A  17       77.52   -153.58                                   
REMARK 500  4 VAL A  22     -136.33   -131.85                                   
REMARK 500  4 LYS A  23      135.15     62.35                                   
REMARK 500  4 ASP A  32     -154.66     71.05                                   
REMARK 500  4 ASP A  39      -71.19    -92.05                                   
REMARK 500  4 ASN A  40      100.86     32.40                                   
REMARK 500  4 LYS A  42      -70.31   -154.58                                   
REMARK 500  4 VAL A  60     -161.36   -110.41                                   
REMARK 500  4 ASP A  69      -37.13   -165.30                                   
REMARK 500  4 GLU A  91        9.86   -160.60                                   
REMARK 500  4 GLU A 102      155.31     45.89                                   
REMARK 500  4 PRO A 106       40.82    -84.94                                   
REMARK 500  4 ASP A 118     -112.94    -39.74                                   
REMARK 500  4 GLU A 119       46.23    -79.75                                   
REMARK 500  4 CYS A 120       35.19    -89.49                                   
REMARK 500  4 GLU A 128      -80.78     59.04                                   
REMARK 500  4 GLU A 129       52.01    -91.42                                   
REMARK 500  4 TYR A 130       60.63     35.44                                   
REMARK 500  4 THR A 132      -86.75     55.90                                   
REMARK 500  5 ASP A  17     -159.43     51.81                                   
REMARK 500  5 ALA A  19      174.16    -58.53                                   
REMARK 500  5 VAL A  22     -123.77     57.62                                   
REMARK 500  5 ASP A  32     -145.36     74.21                                   
REMARK 500  5 ASP A  39     -136.53     28.37                                   
REMARK 500  5 SER A  49       31.87   -158.14                                   
REMARK 500  5 VAL A  60     -167.08   -111.08                                   
REMARK 500  5 GLU A  70       45.08     86.37                                   
REMARK 500  5 GLU A  91       15.36   -162.46                                   
REMARK 500  5 MET A 100       91.66     59.42                                   
REMARK 500  5 CYS A 113      135.41   -174.40                                   
REMARK 500  5 SER A 117      -76.91   -138.42                                   
REMARK 500  5 ASP A 118      128.22    165.47                                   
REMARK 500  5 CYS A 120       35.81    -86.07                                   
REMARK 500  5 SER A 127       29.36   -157.99                                   
REMARK 500  5 GLU A 128      -85.93     55.88                                   
REMARK 500  5 TYR A 130       75.35     52.50                                   
REMARK 500  6 LYS A  23      -67.35   -101.22                                   
REMARK 500  6 PHE A  24      173.75     54.04                                   
REMARK 500  6 PRO A  25      -90.42    -71.13                                   
REMARK 500  6 CYS A  28     -161.83   -162.04                                   
REMARK 500  6 ASP A  32     -151.13     69.56                                   
REMARK 500  6 GLN A  41      -32.07   -162.55                                   
REMARK 500  6 LYS A  42     -145.52   -153.63                                   
REMARK 500  6 SER A  49       19.91   -157.66                                   
REMARK 500  6 GLU A  55      -59.11      1.25                                   
REMARK 500  6 VAL A  60     -169.19   -112.40                                   
REMARK 500  6 ASP A  69      -68.20     67.76                                   
REMARK 500  6 GLU A  70      -17.99   -140.63                                   
REMARK 500  6 PRO A  81     -147.63    -75.45                                   
REMARK 500  6 LYS A  82      -58.37     66.49                                   
REMARK 500  6 GLU A  91       13.86   -163.56                                   
REMARK 500  6 GLU A 102      157.18     45.26                                   
REMARK 500  6 PRO A 106       23.77    -78.57                                   
REMARK 500  6 CYS A 113      112.16   -162.82                                   
REMARK 500  6 GLU A 119      121.36     57.36                                   
REMARK 500  6 CYS A 120      -22.95   -141.31                                   
REMARK 500  6 SER A 127      -36.74   -158.13                                   
REMARK 500  6 GLU A 128     -102.81     54.99                                   
REMARK 500  6 ASN A 131       88.68    -54.90                                   
REMARK 500  6 ASN A 134       71.17     54.68                                   
REMARK 500  7 THR A  16      -68.36     64.88                                   
REMARK 500  7 PHE A  24      164.59     56.63                                   
REMARK 500  7 ASP A  32     -150.36     73.55                                   
REMARK 500  7 ARG A  34     -165.10   -116.76                                   
REMARK 500  7 CYS A  38       72.63    -63.96                                   
REMARK 500  7 SER A  43      154.47     81.99                                   
REMARK 500  7 ASP A  69      -68.55     66.79                                   
REMARK 500  7 LEU A  90       79.79   -161.76                                   
REMARK 500  7 MET A 100       88.10    -68.92                                   
REMARK 500  7 GLU A 102      129.57     74.30                                   
REMARK 500  7 ASP A 118     -105.49    -40.50                                   
REMARK 500  7 GLU A 128      -89.50     53.17                                   
REMARK 500  7 ASN A 131       30.64    -93.91                                   
REMARK 500  8 ALA A  21      -61.14     66.73                                   
REMARK 500  8 ASP A  32     -147.89     67.87                                   
REMARK 500  8 CYS A  38       64.04   -179.69                                   
REMARK 500  8 ASN A  40      126.67   -178.58                                   
REMARK 500  8 GLN A  41       20.97    -79.81                                   
REMARK 500  8 SER A  43      156.68     81.30                                   
REMARK 500  8 ASP A  69      -76.32     57.74                                   
REMARK 500  8 ILE A  89     -142.58    -85.11                                   
REMARK 500  8 LEU A  90      -50.97   -161.60                                   
REMARK 500  8 ASP A  92      -13.69   -149.76                                   
REMARK 500  8 GLU A 102      127.95     78.38                                   
REMARK 500  8 GLU A 119      133.06     59.62                                   
REMARK 500  8 CYS A 120      -24.10   -175.41                                   
REMARK 500  8 SER A 127       53.48   -157.85                                   
REMARK 500  8 GLU A 128      -92.01     55.39                                   
REMARK 500  8 GLU A 129      -61.34   -101.01                                   
REMARK 500  8 TYR A 130       72.77   -169.00                                   
REMARK 500  8 THR A 132       24.47     45.10                                   
REMARK 500  8 SER A 133      -33.03    177.08                                   
REMARK 500  8 ASN A 134       73.61     53.96                                   
REMARK 500  9 PHE A  24      137.93     61.47                                   
REMARK 500  9 ASP A  32     -150.23     70.02                                   
REMARK 500  9 CYS A  38       90.51    -62.02                                   
REMARK 500  9 ASP A  39     -159.88   -112.83                                   
REMARK 500  9 ASN A  40      -96.34     37.67                                   
REMARK 500  9 GLN A  41     -130.74   -163.86                                   
REMARK 500  9 LYS A  42       -5.04     63.52                                   
REMARK 500  9 SER A  43      155.18     74.92                                   
REMARK 500  9 ASP A  69      -82.13     55.12                                   
REMARK 500  9 GLU A  91        9.61   -162.81                                   
REMARK 500  9 GLU A 102      126.60     75.84                                   
REMARK 500  9 GLU A 119       79.66     47.28                                   
REMARK 500  9 SER A 127     -126.03   -157.93                                   
REMARK 500  9 GLU A 128      -86.39     58.65                                   
REMARK 500  9 SER A 133      -21.59     82.38                                   
REMARK 500  9 ASN A 134       54.36     30.35                                   
REMARK 500 10 ASN A  18      152.10     60.44                                   
REMARK 500 10 LYS A  23     -148.55    -67.74                                   
REMARK 500 10 PHE A  24      164.75     61.74                                   
REMARK 500 10 PHE A  30       73.38   -115.87                                   
REMARK 500 10 ASP A  32     -142.85    -41.73                                   
REMARK 500 10 ASP A  39     -139.60   -174.63                                   
REMARK 500 10 GLN A  41      -90.77   -162.73                                   
REMARK 500 10 LYS A  42       46.52   -156.30                                   
REMARK 500 10 SER A  43      156.81     76.66                                   
REMARK 500 10 SER A  49       29.31   -158.56                                   
REMARK 500 10 GLU A  55      -58.35      1.78                                   
REMARK 500 10 VAL A  60     -166.86   -113.68                                   
REMARK 500 10 ASP A  69      -71.15     64.58                                   
REMARK 500 10 GLU A  91       31.20   -159.60                                   
REMARK 500 10 GLU A 102      121.54     77.83                                   
REMARK 500 10 ASP A 118     -111.37    -40.49                                   
REMARK 500 10 GLU A 128      -76.03     59.78                                   
REMARK 500 10 TYR A 130       72.74     53.72                                   
REMARK 500 10 THR A 132     -100.16     50.16                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1 ARG A  34         0.24    SIDE_CHAIN                              
REMARK 500  1 ARG A  66         0.21    SIDE_CHAIN                              
REMARK 500  2 ARG A  34         0.24    SIDE_CHAIN                              
REMARK 500  2 ARG A  66         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  34         0.32    SIDE_CHAIN                              
REMARK 500  3 ARG A  66         0.25    SIDE_CHAIN                              
REMARK 500  4 ARG A  34         0.30    SIDE_CHAIN                              
REMARK 500  4 ARG A  66         0.32    SIDE_CHAIN                              
REMARK 500  5 ARG A  34         0.31    SIDE_CHAIN                              
REMARK 500  5 ARG A  66         0.26    SIDE_CHAIN                              
REMARK 500  6 ARG A  34         0.30    SIDE_CHAIN                              
REMARK 500  6 ARG A  66         0.32    SIDE_CHAIN                              
REMARK 500  7 ARG A  34         0.24    SIDE_CHAIN                              
REMARK 500  7 ARG A  66         0.29    SIDE_CHAIN                              
REMARK 500  8 ARG A  34         0.26    SIDE_CHAIN                              
REMARK 500  8 ARG A  66         0.31    SIDE_CHAIN                              
REMARK 500  9 ARG A  34         0.29    SIDE_CHAIN                              
REMARK 500  9 ARG A  66         0.23    SIDE_CHAIN                              
REMARK 500 10 ARG A  34         0.17    SIDE_CHAIN                              
REMARK 500 10 ARG A  66         0.32    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 4779   RELATED DB: BMRB                                  
REMARK 900 BACKBONE AND SIDECHAIN 1H, 13C, AND 15N NMR CHEMICAL SHIFT           
REMARK 900 ASSIGNMENTS                                                          
DBREF  1PLO A   15   136  UNP    P37173   TGFR2_HUMAN     38    159             
SEQADV 1PLO ALA A   19  UNP  P37173    ASN    42 ENGINEERED                     
SEQRES   1 A  122  VAL THR ASP ASN ALA GLY ALA VAL LYS PHE PRO GLN LEU          
SEQRES   2 A  122  CYS LYS PHE CYS ASP VAL ARG PHE SER THR CYS ASP ASN          
SEQRES   3 A  122  GLN LYS SER CYS MET SER ASN CYS SER ILE THR SER ILE          
SEQRES   4 A  122  CYS GLU LYS PRO GLN GLU VAL CYS VAL ALA VAL TRP ARG          
SEQRES   5 A  122  LYS ASN ASP GLU ASN ILE THR LEU GLU THR VAL CYS HIS          
SEQRES   6 A  122  ASP PRO LYS LEU PRO TYR HIS ASP PHE ILE LEU GLU ASP          
SEQRES   7 A  122  ALA ALA SER PRO LYS CYS ILE MET LYS GLU LYS LYS LYS          
SEQRES   8 A  122  PRO GLY GLU THR PHE PHE MET CYS SER CYS SER SER ASP          
SEQRES   9 A  122  GLU CYS ASN ASP ASN ILE ILE PHE SER GLU GLU TYR ASN          
SEQRES  10 A  122  THR SER ASN PRO ASP                                          
SHEET    1   A 2 LEU A  27  LYS A  29  0                                        
SHEET    2   A 2 THR A  51  ILE A  53 -1  O  SER A  52   N  CYS A  28           
SHEET    1   B 4 ASP A  32  PHE A  35  0                                        
SHEET    2   B 4 ILE A  72  HIS A  79 -1  O  LEU A  74   N  ARG A  34           
SHEET    3   B 4 VAL A  60  LYS A  67 -1  N  VAL A  60   O  HIS A  79           
SHEET    4   B 4 THR A 109  PHE A 110 -1  O  THR A 109   N  LYS A  67           
SHEET    1   C 4 ASP A  32  PHE A  35  0                                        
SHEET    2   C 4 ILE A  72  HIS A  79 -1  O  LEU A  74   N  ARG A  34           
SHEET    3   C 4 VAL A  60  LYS A  67 -1  N  VAL A  60   O  HIS A  79           
SHEET    4   C 4 SER A 114  CYS A 115 -1  O  CYS A 115   N  CYS A  61           
SHEET    1   D 2 CYS A  44  MET A  45  0                                        
SHEET    2   D 2 ASN A 123  ILE A 124 -1  O  ILE A 124   N  CYS A  44           
SSBOND   1 CYS A   28    CYS A   61                          1555   1555  2.02  
SSBOND   2 CYS A   31    CYS A   48                          1555   1555  2.02  
SSBOND   3 CYS A   38    CYS A   44                          1555   1555  2.02  
SSBOND   4 CYS A   54    CYS A   78                          1555   1555  2.02  
SSBOND   5 CYS A   98    CYS A  113                          1555   1555  2.02  
SSBOND   6 CYS A  115    CYS A  120                          1555   1555  2.02  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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