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Database: PDB
Entry: 1RGO
LinkDB: 1RGO
Original site: 1RGO 
HEADER    RNA BINDING PROTEIN                     12-NOV-03   1RGO              
TITLE     STRUCTURAL BASIS FOR RECOGNITION OF THE MRNA CLASS II AU-             
TITLE    2 RICH ELEMENT BY THE TANDEM ZINC FINGER DOMAIN OF TIS11D              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: BUTYRATE RESPONSE FACTOR 2;                                
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: TIS11D PROTEIN, EGF-RESPONSE FACTOR 2, ERF-2;               
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MOL_ID: 2;                                                           
COMPND   7 MOLECULE: RNA (5'-R(*UP*UP*AP*UP*UP*UP*AP*UP*U)-3');                 
COMPND   8 CHAIN: D;                                                            
COMPND   9 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ZFP36L2, BRF2, TIS11D, ERF2;                                   
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET21A;                                   
SOURCE  11 MOL_ID: 2;                                                           
SOURCE  12 SYNTHETIC: YES;                                                      
SOURCE  13 OTHER_DETAILS: SYNTHETIC                                             
KEYWDS    TIS11 TTP TRISTETRAPROLIN BUTYRATE RESPONSE FACTOR ERF                
KEYWDS   2 NUP475 ZFP ZN ZINC FINGER RNA SS SINGLE-STRANDED ARE UTR             
KEYWDS   3 TANDEM INTERCALATION INTERCALATE SPECIFIC, RNA BINDING               
KEYWDS   4 PROTEIN                                                              
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    B.P.HUDSON,M.A.MARTINEZ-YAMOUT,H.J.DYSON,P.E.WRIGHT                   
REVDAT   2   24-FEB-09 1RGO    1       VERSN                                    
REVDAT   1   02-MAR-04 1RGO    0                                                
JRNL        AUTH   B.P.HUDSON,M.A.MARTINEZ-YAMOUT,H.J.DYSON,P.E.WRIGHT          
JRNL        TITL   RECOGNITION OF THE MRNA AU-RICH ELEMENT BY THE               
JRNL        TITL 2 ZINC FINGER DOMAIN OF TIS11D.                                
JRNL        REF    NAT.STRUCT.MOL.BIOL.          V.  11   257 2004              
JRNL        REFN                   ISSN 1545-9993                               
JRNL        PMID   14981510                                                     
JRNL        DOI    10.1038/NSMB738                                              
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : AMBER 8                                              
REMARK   3   AUTHORS     : CASE, D. A., ET AL.                                  
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1RGO COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 05-DEC-03.                  
REMARK 100 THE RCSB ID CODE IS RCSB020729.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 6.2                                
REMARK 210  IONIC STRENGTH                 : 20 MM KCL                          
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.5 MM 15N-TIS11D TZF; 0.5 MM      
REMARK 210                                   5'-UUAUUUAUU-3'; 10 MM TRIS PH     
REMARK 210                                   6.2; 20 MM KCL; 2.5 MM DTT; 25     
REMARK 210                                   UM ZNSO4; 0.5 MM 15N,13C-          
REMARK 210                                   TIS11D TZF; 0.5 MM 5'-             
REMARK 210                                   UUAUUUAUU-3'; 10 MM TRIS PH        
REMARK 210                                   6.2; 20 MM KCL; 2.5 MM DTT; 25     
REMARK 210                                   UM ZNSO4; 0.5 MM 15N,13C-          
REMARK 210                                   TIS11D TZF; 0.5 MM 5'-             
REMARK 210                                   UUAUUUAUU-3'; 10 MM TRIS-D11       
REMARK 210                                   PD 6.2; 20 MM KCL; 2.5 MM DTT-     
REMARK 210                                   D10; 25 UM ZNSO4; 0.5 MM 15N,      
REMARK 210                                   13C-TIS11D TZF; 0.5 MM 5'-         
REMARK 210                                   UUAUUUAUU-3'; 10 MM TRIS PH        
REMARK 210                                   6.2; 20 MM KCL; 2.5 MM DTT; 25     
REMARK 210                                   UM ZNSO4; 12 MG/ML PF1             
REMARK 210                                   BACTERIOPHAGE                      
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY, 3D 13C        
REMARK 210                                   F1-EDITED, F3-FILTERED NOESY,      
REMARK 210                                   2D 13C DOUBLE-HALF-FILTERED        
REMARK 210                                   NOESY, HNHA, HNHB, HACAHB          
REMARK 210                                   COSY, 13C-{13CO} SPIN-ECHO         
REMARK 210                                   DIFFERENCE CT-HSQC, 13C-{15N}      
REMARK 210                                   SPIN-ECHO DIFFERENCE CT-HSQC,      
REMARK 210                                   IPAP-[1H-15N]-HSQC                 
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 750 MHZ, 900 MHZ          
REMARK 210  SPECTROMETER MODEL             : AVANCE, DMX                        
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR VARIOUS, NMRPIPE,          
REMARK 210                                   NMRVIEW 5.0, SANE, DYANA 1.5,      
REMARK 210                                   AMBER 8                            
REMARK 210   METHOD USED                   : INDIVIDUAL ZINC FINGER             
REMARK 210                                   DOMAINS WERE CREATED IN DYANA      
REMARK 210                                   AND DOCKED TO UAUU (FINGER I)      
REMARK 210                                   AND UUAUU (FINGER II) USING        
REMARK 210                                   FIVE ROUNDS OF SIMULATED           
REMARK 210                                   ANNEALING IN AMBER USING           
REMARK 210                                   DISTANCE AND TORSIONAL             
REMARK 210                                   RESTRAINTS. FINGER I/UAUU AND      
REMARK 210                                   FINGER II/UUAUU COMPLEXES WERE     
REMARK 210                                   CONNECTED TO FORM TZF/             
REMARK 210                                   UUAUUUAUU COMPLEXES AND            
REMARK 210                                   REFINED IN AMBER WITH RESIDUAL     
REMARK 210                                   DIPOLAR COUPLING RESTRAINTS        
REMARK 210                                   ADDED. CALCULATIONS WERE THEN      
REMARK 210                                   SWITCHED FROM IN VACUO TO          
REMARK 210                                   GENERALIZED BORN CONTINUUM         
REMARK 210                                   SOLVENT MODEL AND ONE              
REMARK 210                                   ADDITIONAL ROUND OF SIMULATED      
REMARK 210                                   ANNEALING WAS PERFORMED.           
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 200                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1   A D   3   C4  -  C5  -  C6  ANGL. DEV. =  -3.0 DEGREES          
REMARK 500  1   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.6 DEGREES          
REMARK 500  1   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -5.2 DEGREES          
REMARK 500  1   A D   7   C4  -  C5  -  C6  ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  1   A D   7   C5  -  C6  -  N1  ANGL. DEV. =   3.9 DEGREES          
REMARK 500  1   A D   7   N1  -  C6  -  N6  ANGL. DEV. =  -4.2 DEGREES          
REMARK 500  1 ARG A 184   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  2   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.7 DEGREES          
REMARK 500  2   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -4.8 DEGREES          
REMARK 500  2   A D   7   C4  -  C5  -  C6  ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  2   A D   7   C5  -  C6  -  N1  ANGL. DEV. =   3.9 DEGREES          
REMARK 500  2   A D   7   N1  -  C6  -  N6  ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  3   A D   3   C4  -  C5  -  C6  ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  3   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.6 DEGREES          
REMARK 500  3   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -5.3 DEGREES          
REMARK 500  3   A D   7   C4  -  C5  -  C6  ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  3   A D   7   C5  -  C6  -  N1  ANGL. DEV. =   4.0 DEGREES          
REMARK 500  3   A D   7   N1  -  C6  -  N6  ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  3   U D   8   O4' -  C1' -  N1  ANGL. DEV. =   4.3 DEGREES          
REMARK 500  3 ARG A 188   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500  3 ARG A 198   NE  -  CZ  -  NH1 ANGL. DEV. =   3.2 DEGREES          
REMARK 500  4   A D   3   C4  -  C5  -  C6  ANGL. DEV. =  -3.3 DEGREES          
REMARK 500  4   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.5 DEGREES          
REMARK 500  4   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -5.4 DEGREES          
REMARK 500  4   U D   4   O4' -  C1' -  N1  ANGL. DEV. =   4.8 DEGREES          
REMARK 500  4   U D   6   C1' -  O4' -  C4' ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  4   U D   6   O4' -  C1' -  N1  ANGL. DEV. =   5.4 DEGREES          
REMARK 500  4   A D   7   C5  -  C6  -  N1  ANGL. DEV. =   3.9 DEGREES          
REMARK 500  4   A D   7   N1  -  C6  -  N6  ANGL. DEV. =  -4.4 DEGREES          
REMARK 500  4 ARG A 153   NE  -  CZ  -  NH1 ANGL. DEV. =   3.1 DEGREES          
REMARK 500  5   A D   3   C4  -  C5  -  C6  ANGL. DEV. =  -3.2 DEGREES          
REMARK 500  5   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.5 DEGREES          
REMARK 500  5   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -5.5 DEGREES          
REMARK 500  5   U D   6   O4' -  C1' -  N1  ANGL. DEV. =   4.2 DEGREES          
REMARK 500  5   A D   7   C4  -  C5  -  C6  ANGL. DEV. =  -3.0 DEGREES          
REMARK 500  5   A D   7   C5  -  C6  -  N1  ANGL. DEV. =   3.7 DEGREES          
REMARK 500  5   A D   7   N1  -  C6  -  N6  ANGL. DEV. =  -4.3 DEGREES          
REMARK 500  5   U D   8   O4' -  C1' -  N1  ANGL. DEV. =   4.6 DEGREES          
REMARK 500  6   U D   2   C1' -  O4' -  C4' ANGL. DEV. =  -5.0 DEGREES          
REMARK 500  6   U D   2   O4' -  C1' -  N1  ANGL. DEV. =   5.4 DEGREES          
REMARK 500  6   A D   3   C4  -  C5  -  C6  ANGL. DEV. =  -3.1 DEGREES          
REMARK 500  6   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.7 DEGREES          
REMARK 500  6   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -5.1 DEGREES          
REMARK 500  6   A D   7   C5  -  C6  -  N1  ANGL. DEV. =   3.8 DEGREES          
REMARK 500  6   A D   7   N1  -  C6  -  N6  ANGL. DEV. =  -4.4 DEGREES          
REMARK 500  6 ARG A 184   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500  6 ARG A 198   NE  -  CZ  -  NH1 ANGL. DEV. =   3.4 DEGREES          
REMARK 500  7   U D   2   O4' -  C1' -  N1  ANGL. DEV. =   4.4 DEGREES          
REMARK 500  7   A D   3   C5  -  C6  -  N1  ANGL. DEV. =   3.4 DEGREES          
REMARK 500  7   A D   3   N1  -  C6  -  N6  ANGL. DEV. =  -5.2 DEGREES          
REMARK 500                                                                      
REMARK 500 THIS ENTRY HAS     142 ANGLE DEVIATIONS.                             
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 HIS A 216      -94.09    -88.59                                   
REMARK 500  2 HIS A 216      -95.01    -86.65                                   
REMARK 500  3 HIS A 216      -95.75    -89.64                                   
REMARK 500  4 HIS A 216      -95.62    -91.70                                   
REMARK 500  5 HIS A 216      -90.23    -85.98                                   
REMARK 500  6 HIS A 216      -93.96    -93.46                                   
REMARK 500  7 HIS A 216      -97.28    -96.00                                   
REMARK 500  8 HIS A 216      -95.26    -89.38                                   
REMARK 500  9 HIS A 216      -97.29    -83.77                                   
REMARK 500 10 HIS A 216      -98.46    -87.19                                   
REMARK 500 11 HIS A 216      -95.26    -90.71                                   
REMARK 500 12 HIS A 216      -92.57    -82.79                                   
REMARK 500 12 ALA A 218       97.24    -69.33                                   
REMARK 500 13 HIS A 216      -94.61    -89.72                                   
REMARK 500 14 HIS A 216      -97.53    -87.24                                   
REMARK 500 15 HIS A 216      -94.50    -90.72                                   
REMARK 500 16 HIS A 216      -94.50    -89.80                                   
REMARK 500 17 ARG A 153       42.08    -82.20                                   
REMARK 500 17 HIS A 216      -89.17    -88.52                                   
REMARK 500 18 HIS A 216      -87.30    -89.54                                   
REMARK 500 18 ALA A 218      -94.51    -92.35                                   
REMARK 500 19 HIS A 216      -89.78    -82.79                                   
REMARK 500 20 ARG A 153       41.66    -81.10                                   
REMARK 500 20 HIS A 216      -87.72    -91.74                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  1   A D   7         0.10    SIDE_CHAIN                              
REMARK 500  2   U D   5         0.07    SIDE_CHAIN                              
REMARK 500  2   A D   7         0.07    SIDE_CHAIN                              
REMARK 500  4   U D   4         0.08    SIDE_CHAIN                              
REMARK 500  4   U D   5         0.06    SIDE_CHAIN                              
REMARK 500  4   A D   7         0.08    SIDE_CHAIN                              
REMARK 500  6   A D   3         0.06    SIDE_CHAIN                              
REMARK 500  6   A D   7         0.06    SIDE_CHAIN                              
REMARK 500  7   U D   5         0.08    SIDE_CHAIN                              
REMARK 500  7   A D   7         0.11    SIDE_CHAIN                              
REMARK 500  8   A D   3         0.08    SIDE_CHAIN                              
REMARK 500  8   U D   6         0.07    SIDE_CHAIN                              
REMARK 500  8   A D   7         0.08    SIDE_CHAIN                              
REMARK 500  9   A D   7         0.08    SIDE_CHAIN                              
REMARK 500 10   U D   2         0.07    SIDE_CHAIN                              
REMARK 500 10   U D   6         0.06    SIDE_CHAIN                              
REMARK 500 10   A D   7         0.07    SIDE_CHAIN                              
REMARK 500 11   U D   2         0.07    SIDE_CHAIN                              
REMARK 500 11   A D   3         0.06    SIDE_CHAIN                              
REMARK 500 11   U D   6         0.10    SIDE_CHAIN                              
REMARK 500 11   A D   7         0.07    SIDE_CHAIN                              
REMARK 500 12   A D   3         0.09    SIDE_CHAIN                              
REMARK 500 12   U D   6         0.07    SIDE_CHAIN                              
REMARK 500 12   A D   7         0.06    SIDE_CHAIN                              
REMARK 500 13   U D   6         0.07    SIDE_CHAIN                              
REMARK 500 13 TYR A 170         0.08    SIDE_CHAIN                              
REMARK 500 14   U D   2         0.07    SIDE_CHAIN                              
REMARK 500 14   U D   6         0.08    SIDE_CHAIN                              
REMARK 500 14   A D   7         0.10    SIDE_CHAIN                              
REMARK 500 15   U D   2         0.15    SIDE_CHAIN                              
REMARK 500 15   A D   7         0.07    SIDE_CHAIN                              
REMARK 500 16   U D   2         0.08    SIDE_CHAIN                              
REMARK 500 16   A D   7         0.06    SIDE_CHAIN                              
REMARK 500 17   U D   6         0.08    SIDE_CHAIN                              
REMARK 500 17   A D   7         0.08    SIDE_CHAIN                              
REMARK 500 18   U D   6         0.06    SIDE_CHAIN                              
REMARK 500 18   A D   7         0.07    SIDE_CHAIN                              
REMARK 500 19   U D   6         0.08    SIDE_CHAIN                              
REMARK 500 19   A D   7         0.07    SIDE_CHAIN                              
REMARK 500 20   A D   3         0.08    SIDE_CHAIN                              
REMARK 500 20   U D   6         0.08    SIDE_CHAIN                              
REMARK 500 20   A D   7         0.07    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 620                                                                      
REMARK 620 METAL COORDINATION                                                   
REMARK 620  (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;              
REMARK 620  SSEQ=SEQUENCE NUMBER; I=INSERTION CODE):                            
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                           1  ZN A 221  ZN                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 CYS A 174   SG                                                     
REMARK 620 2 CYS A 159   SG  108.5                                              
REMARK 620 3 CYS A 168   SG  108.1 112.1                                        
REMARK 620 4 HIS A 178   NE2 108.5 112.2 107.3                                  
REMARK 620 N                    1     2     3                                   
REMARK 620                                                                      
REMARK 620 COORDINATION ANGLES FOR:  M RES CSSEQI METAL                         
REMARK 620                           1  ZN A 222  ZN                            
REMARK 620 N RES CSSEQI ATOM                                                    
REMARK 620 1 CYS A 197   SG                                                     
REMARK 620 2 CYS A 212   SG  109.6                                              
REMARK 620 3 CYS A 206   SG  111.1 110.4                                        
REMARK 620 4 HIS A 216   NE2 111.8 106.6 107.2                                  
REMARK 620 N                    1     2     3                                   
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN A 221                  
REMARK 800 SITE_IDENTIFIER: AC2                                                 
REMARK 800 EVIDENCE_CODE: SOFTWARE                                              
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE ZN A 222                  
DBREF  1RGO A  151   220  UNP    P47974   TISD_HUMAN     151    220             
DBREF  1RGO D    1     9  PDB    1RGO     1RGO             1      9             
SEQRES   1 A   70  SER THR ARG TYR LYS THR GLU LEU CYS ARG PRO PHE GLU          
SEQRES   2 A   70  GLU SER GLY THR CYS LYS TYR GLY GLU LYS CYS GLN PHE          
SEQRES   3 A   70  ALA HIS GLY PHE HIS GLU LEU ARG SER LEU THR ARG HIS          
SEQRES   4 A   70  PRO LYS TYR LYS THR GLU LEU CYS ARG THR PHE HIS THR          
SEQRES   5 A   70  ILE GLY PHE CYS PRO TYR GLY PRO ARG CYS HIS PHE ILE          
SEQRES   6 A   70  HIS ASN ALA ASP GLU                                          
SEQRES   1 D    9    U   U   A   U   U   U   A   U   U                          
HET     ZN  A 221       1                                                       
HET     ZN  A 222       1                                                       
HETNAM      ZN ZINC ION                                                         
FORMUL   3   ZN    2(ZN 2+)                                                     
HELIX    1   1 CYS A  159  GLY A  166  1                                   8    
HELIX    2   2 TYR A  170  CYS A  174  5                                   5    
HELIX    3   3 GLY A  179  LEU A  183  5                                   5    
HELIX    4   4 CYS A  197  GLY A  204  1                                   8    
HELIX    5   5 TYR A  208  CYS A  212  5                                   5    
LINK        ZN    ZN A 221                 SG  CYS A 174     1555   1555  2.28  
LINK        ZN    ZN A 221                 SG  CYS A 159     1555   1555  2.30  
LINK        ZN    ZN A 221                 SG  CYS A 168     1555   1555  2.29  
LINK        ZN    ZN A 221                 NE2 HIS A 178     1555   1555  2.09  
LINK        ZN    ZN A 222                 SG  CYS A 197     1555   1555  2.30  
LINK        ZN    ZN A 222                 SG  CYS A 212     1555   1555  2.29  
LINK        ZN    ZN A 222                 SG  CYS A 206     1555   1555  2.29  
LINK        ZN    ZN A 222                 NE2 HIS A 216     1555   1555  2.08  
SITE     1 AC1  4 CYS A 159  CYS A 168  CYS A 174  HIS A 178                    
SITE     1 AC2  4 CYS A 197  CYS A 206  CYS A 212  HIS A 216                    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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