HEADER RIBOSOME 07-DEC-03 1RQS
TITLE NMR STRUCTURE OF C-TERMINAL DOMAIN OF RIBOSOMAL PROTEIN L7 FROM E.COLI
COMPND MOL_ID: 1;
COMPND 2 MOLECULE: 50S RIBOSOMAL PROTEIN L7/L12;
COMPND 3 CHAIN: A;
COMPND 4 FRAGMENT: C-TERMINAL DOMAIN;
COMPND 5 SYNONYM: L8;
COMPND 6 ENGINEERED: YES
SOURCE MOL_ID: 1;
SOURCE 2 ORGANISM_SCIENTIFIC: ESCHERICHIA COLI, ESCHERICHIA COLI O6,
SOURCE 3 ESCHERICHIA COLI O157:H7, SHIGELLA FLEXNERI;
SOURCE 4 ORGANISM_COMMON: , , ,;
SOURCE 5 ORGANISM_TAXID: 562,217992,83334,623;
SOURCE 6 STRAIN: , O6, O157:H7,;
SOURCE 7 EXPRESSION_SYSTEM: ESCHERICHIA COLI;
SOURCE 8 EXPRESSION_SYSTEM_TAXID: 562;
SOURCE 9 EXPRESSION_SYSTEM_STRAIN: XL1;
SOURCE 10 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE 11 EXPRESSION_SYSTEM_PLASMID: PPR1
KEYWDS PROTEIN L7/L12, RIBOSOME
EXPDTA SOLUTION NMR
NUMMDL 20
AUTHOR E.V.BOCHAROV,A.G.SOBOL,K.V.PAVLOV,D.M.KORZHNEV,V.A.JARAVINE,
AUTHOR 2 A.T.GUDKOV,A.S.ARSENIEV
REVDAT 4 02-MAR-22 1RQS 1 REMARK
REVDAT 3 24-FEB-09 1RQS 1 VERSN
REVDAT 2 22-JUN-04 1RQS 1 JRNL
REVDAT 1 02-MAR-04 1RQS 0
JRNL AUTH E.V.BOCHAROV,A.G.SOBOL,K.V.PAVLOV,D.M.KORZHNEV,V.A.JARAVINE,
JRNL AUTH 2 A.T.GUDKOV,A.S.ARSENIEV
JRNL TITL FROM STRUCTURE AND DYNAMICS OF PROTEIN L7/L12 TO MOLECULAR
JRNL TITL 2 SWITCHING IN RIBOSOME.
JRNL REF J.BIOL.CHEM. V. 279 17697 2004
JRNL REFN ISSN 0021-9258
JRNL PMID 14960595
JRNL DOI 10.1074/JBC.M313384200
REMARK 2
REMARK 2 RESOLUTION. NOT APPLICABLE.
REMARK 3
REMARK 3 REFINEMENT.
REMARK 3 PROGRAM : VNMR 6.3B, FANTOM 4
REMARK 3 AUTHORS : VARIAN USA (VNMR), SCHAUMANN, T. (FANTOM)
REMARK 3
REMARK 3 OTHER REFINEMENT REMARKS: NULL
REMARK 4
REMARK 4 1RQS COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 09-DEC-03.
REMARK 100 THE DEPOSITION ID IS D_1000020966.
REMARK 210
REMARK 210 EXPERIMENTAL DETAILS
REMARK 210 EXPERIMENT TYPE : NMR
REMARK 210 TEMPERATURE (KELVIN) : 303
REMARK 210 PH : 6.9
REMARK 210 IONIC STRENGTH : 0.15
REMARK 210 PRESSURE : AMBIENT
REMARK 210 SAMPLE CONTENTS : 1MM L7 DIMER U-15N; 50MM
REMARK 210 PHOSPHATE BUFFER; 90% H2O, 10%
REMARK 210 D2O; 30 C; 1MM L7 DIMER U-15N;
REMARK 210 50MM PHOSPHATE BUFFER; 99.9% D2O;
REMARK 210 30 C; 1MM L7 DIMER U-15N; 50MM
REMARK 210 PHOSPHATE BUFFER; 90% H2O, 10%
REMARK 210 D2O; 30 C; TOBACCO VIRUS
REMARK 210 ALIGNMENT MEDIUM
REMARK 210
REMARK 210 NMR EXPERIMENTS CONDUCTED : 3D_15N-SEPARATED_NOESY; 2D
REMARK 210 NOESY; 3D_15N-HNHB; 15N-HMQCJ;
REMARK 210 15N-JNH_MODULATED_HSQC
REMARK 210 SPECTROMETER FIELD STRENGTH : 600 MHZ
REMARK 210 SPECTROMETER MODEL : UNITY
REMARK 210 SPECTROMETER MANUFACTURER : VARIAN
REMARK 210
REMARK 210 STRUCTURE DETERMINATION.
REMARK 210 SOFTWARE USED : XEASY 1.2.11, CYANA 1.01
REMARK 210 METHOD USED : SIMULATED ANNEALING COMBINED
REMARK 210 WITH MOLECULAR DYNAMICS IN
REMARK 210 TORSION ANGLE SPACE
REMARK 210
REMARK 210 CONFORMERS, NUMBER CALCULATED : 200
REMARK 210 CONFORMERS, NUMBER SUBMITTED : 20
REMARK 210 CONFORMERS, SELECTION CRITERIA : STRUCTURES WITH THE LEAST
REMARK 210 RESTRAINT VIOLATIONS,TARGET
REMARK 210 FUNCTION
REMARK 210
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 9
REMARK 210
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR
REMARK 210 SPECTROSCOPY
REMARK 215
REMARK 215 NMR STUDY
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION
REMARK 215 NMR DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE THAT
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON
REMARK 215 THESE RECORDS ARE MEANINGLESS.
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000
REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000
REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500 M RES CSSEQI PSI PHI
REMARK 500 1 ALA A 63 38.34 -90.14
REMARK 500 1 ASN A 64 57.60 -154.56
REMARK 500 1 THR A 76 -76.42 -120.98
REMARK 500 2 ALA A 63 38.50 -91.29
REMARK 500 2 ASN A 64 56.52 -153.96
REMARK 500 2 VAL A 72 -73.59 -60.17
REMARK 500 2 THR A 76 -75.77 -116.55
REMARK 500 3 ALA A 63 37.54 -91.52
REMARK 500 3 ASN A 64 59.27 -154.63
REMARK 500 3 VAL A 72 -74.48 -59.17
REMARK 500 3 THR A 76 -72.65 -116.60
REMARK 500 3 ALA A 90 151.66 -49.21
REMARK 500 4 ALA A 63 38.39 -89.04
REMARK 500 4 ASN A 64 58.67 -154.50
REMARK 500 4 THR A 76 -76.16 -121.44
REMARK 500 5 ALA A 48 98.07 -66.87
REMARK 500 5 ALA A 63 39.49 -91.21
REMARK 500 5 ASN A 64 54.77 -153.13
REMARK 500 5 VAL A 72 -71.35 -59.46
REMARK 500 5 THR A 76 -75.36 -117.93
REMARK 500 6 ALA A 48 84.85 -150.83
REMARK 500 6 ALA A 63 37.72 -91.04
REMARK 500 6 ASN A 64 57.10 -154.63
REMARK 500 6 THR A 76 -73.43 -114.12
REMARK 500 7 ALA A 63 37.95 -88.65
REMARK 500 7 ASN A 64 61.81 -154.35
REMARK 500 7 THR A 76 -76.44 -115.88
REMARK 500 8 ALA A 48 66.93 -150.08
REMARK 500 8 ALA A 63 37.19 -90.47
REMARK 500 8 ASN A 64 57.98 -154.01
REMARK 500 8 VAL A 72 -70.43 -60.07
REMARK 500 8 THR A 76 -76.78 -119.89
REMARK 500 9 ALA A 63 38.70 -91.03
REMARK 500 9 ASN A 64 55.65 -154.53
REMARK 500 9 VAL A 72 -71.39 -57.41
REMARK 500 9 THR A 76 -74.56 -118.05
REMARK 500 10 ALA A 63 38.56 -90.79
REMARK 500 10 ASN A 64 55.37 -152.24
REMARK 500 10 VAL A 72 -70.73 -59.06
REMARK 500 10 THR A 76 -76.25 -118.51
REMARK 500 11 ALA A 48 89.25 -158.42
REMARK 500 11 ALA A 63 38.97 -91.87
REMARK 500 11 ASN A 64 54.96 -153.77
REMARK 500 11 THR A 76 -77.78 -118.51
REMARK 500 11 ALA A 90 151.15 -48.69
REMARK 500 12 ALA A 48 102.71 -168.63
REMARK 500 12 LYS A 51 76.42 -103.48
REMARK 500 12 ALA A 63 37.27 -88.96
REMARK 500 12 ASN A 64 55.71 -153.78
REMARK 500 12 THR A 76 -74.76 -120.73
REMARK 500
REMARK 500 THIS ENTRY HAS 87 RAMACHANDRAN OUTLIERS.
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 M RES CSSEQI RMS TYPE
REMARK 500 6 ARG A 73 0.12 SIDE CHAIN
REMARK 500
REMARK 500 REMARK: NULL
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 4429 RELATED DB: BMRB
REMARK 900 RELATED ID: 1CTF RELATED DB: PDB
REMARK 900 RELATED ID: 1DD3 RELATED DB: PDB
REMARK 900 RELATED ID: 1DD4 RELATED DB: PDB
REMARK 900 RELATED ID: 1RQT RELATED DB: PDB
REMARK 900 RELATED ID: 1RQU RELATED DB: PDB
REMARK 900 RELATED ID: 1RQV RELATED DB: PDB
DBREF 1RQS A 47 120 UNP P0A7K2 RL7_ECOLI 47 120
SEQRES 1 A 74 ALA ALA GLU GLU LYS THR GLU PHE ASP VAL ILE LEU LYS
SEQRES 2 A 74 ALA ALA GLY ALA ASN LYS VAL ALA VAL ILE LYS ALA VAL
SEQRES 3 A 74 ARG GLY ALA THR GLY LEU GLY LEU LYS GLU ALA LYS ASP
SEQRES 4 A 74 LEU VAL GLU SER ALA PRO ALA ALA LEU LYS GLU GLY VAL
SEQRES 5 A 74 SER LYS ASP ASP ALA GLU ALA LEU LYS LYS ALA LEU GLU
SEQRES 6 A 74 GLU ALA GLY ALA GLU VAL GLU VAL LYS
HELIX 1 1 ASN A 64 THR A 76 1 13
HELIX 2 2 GLY A 79 SER A 89 1 11
HELIX 3 3 SER A 99 GLY A 114 1 16
SHEET 1 A 3 ALA A 92 VAL A 98 0
SHEET 2 A 3 PHE A 54 ALA A 60 -1 N PHE A 54 O VAL A 98
SHEET 3 A 3 GLU A 116 LYS A 120 -1 O GLU A 116 N ALA A 60
CISPEP 1 ALA A 90 PRO A 91 1 -0.46
CISPEP 2 ALA A 90 PRO A 91 2 -0.06
CISPEP 3 ALA A 90 PRO A 91 3 -0.80
CISPEP 4 ALA A 90 PRO A 91 4 -0.56
CISPEP 5 ALA A 90 PRO A 91 5 -0.81
CISPEP 6 ALA A 90 PRO A 91 6 -0.61
CISPEP 7 ALA A 90 PRO A 91 7 0.15
CISPEP 8 ALA A 90 PRO A 91 8 0.30
CISPEP 9 ALA A 90 PRO A 91 9 -0.19
CISPEP 10 ALA A 90 PRO A 91 10 -0.51
CISPEP 11 ALA A 90 PRO A 91 11 -0.45
CISPEP 12 ALA A 90 PRO A 91 12 -0.28
CISPEP 13 ALA A 90 PRO A 91 13 0.42
CISPEP 14 ALA A 90 PRO A 91 14 -1.10
CISPEP 15 ALA A 90 PRO A 91 15 -0.06
CISPEP 16 ALA A 90 PRO A 91 16 0.05
CISPEP 17 ALA A 90 PRO A 91 17 -0.93
CISPEP 18 ALA A 90 PRO A 91 18 0.18
CISPEP 19 ALA A 90 PRO A 91 19 -0.78
CISPEP 20 ALA A 90 PRO A 91 20 -0.16
CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 1
ORIGX1 1.000000 0.000000 0.000000 0.00000
ORIGX2 0.000000 1.000000 0.000000 0.00000
ORIGX3 0.000000 0.000000 1.000000 0.00000
SCALE1 1.000000 0.000000 0.000000 0.00000
SCALE2 0.000000 1.000000 0.000000 0.00000
SCALE3 0.000000 0.000000 1.000000 0.00000
MODEL 1
(ATOM LINES ARE NOT SHOWN.)
END
