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Database: PDB
Entry: 1SXE
LinkDB: 1SXE
Original site: 1SXE 
HEADER    TRANSCRIPTION, SIGNALING PROTEIN        30-MAR-04   1SXE              
TITLE     THE SOLUTION STRUCTURE OF THE POINTED (PNT) DOMAIN FROM THE           
TITLE    2 TRANSCRITION FACTOR ERG                                              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: TRANSCRIPTIONAL REGULATOR ERG;                             
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: PNT DOMAIN;                                                
COMPND   5 SYNONYM: TRANSFORMING PROTEIN ERG;                                   
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: ERG;                                                           
SOURCE   6 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21(DE3);                       
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 469008;                                     
SOURCE   8 EXPRESSION_SYSTEM_STRAIN: BL21(DE3);                                 
SOURCE   9 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE  10 EXPRESSION_SYSTEM_PLASMID: PET28                                     
KEYWDS    ALPHA HELICAL, TRANSCRIPTION, SIGNALING PROTEIN                       
EXPDTA    SOLUTION NMR                                                          
NUMMDL    14                                                                    
AUTHOR    C.D.MACKERETH,M.SCHAERPF,L.N.GENTILE,S.E.MACINTOSH,                   
AUTHOR   2 C.M.SLUPSKY,L.P.MCINTOSH                                             
REVDAT   2   24-FEB-09 1SXE    1       VERSN                                    
REVDAT   1   21-SEP-04 1SXE    0                                                
JRNL        AUTH   C.D.MACKERETH,M.SCHAERPF,L.N.GENTILE,S.E.MACINTOSH,          
JRNL        AUTH 2 C.M.SLUPSKY,L.P.MCINTOSH                                     
JRNL        TITL   DIVERSITY IN STRUCTURE AND FUNCTION OF THE ETS               
JRNL        TITL 2 FAMILY PNT DOMAINS.                                          
JRNL        REF    J.MOL.BIOL.                   V. 342  1249 2004              
JRNL        REFN                   ISSN 0022-2836                               
JRNL        PMID   15351649                                                     
JRNL        DOI    10.1016/J.JMB.2004.07.094                                    
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : ARIA 1.2                                             
REMARK   3   AUTHORS     : J.LINGE, S.O'DONOGHUE, M.NILGES                      
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1SXE COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-APR-04.                  
REMARK 100 THE RCSB ID CODE IS RCSB022065.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 303; 303; 303; 303                 
REMARK 210  PH                             : 7.0; 7.0; 7.0; 7.0                 
REMARK 210  IONIC STRENGTH                 : 70 MM; 70 MM; 70 MM; 70 MM         
REMARK 210  PRESSURE                       : AMBIENT; AMBIENT; AMBIENT;         
REMARK 210                                   AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1MM ERG U-15N,13C, 20MM            
REMARK 210                                   PHOSPHATE BUFFER PH 7.0, 50MM      
REMARK 210                                   NACL, 2MM DTT, 90% H2O, 10%        
REMARK 210                                   D2O; 1MM ERG 100%15N,10%13C,       
REMARK 210                                   20MM PHOSPHATE BUFFER PH 7.0,      
REMARK 210                                   50MM NACL, 2MM DTT, 90% H2O,       
REMARK 210                                   10% D2O; 2MM ERG 100%15N, 20MM     
REMARK 210                                   PHOSPHATE BUFFER PH 7.0, 50MM      
REMARK 210                                   NACL, 2MM DTT, 90% H2O, 10%        
REMARK 210                                   D2O; 2MM ERG 100%15N, 20MM         
REMARK 210                                   PHOSPHATE BUFFER PH 7.0, 50MM      
REMARK 210                                   NACL, 2MM DTT, 1% H2O, 99% D2O     
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY             
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ                   
REMARK 210  SPECTROMETER MODEL             : UNITY, INOVA                       
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : FELIX 2000, NMRPIPE 1995,          
REMARK 210                                   SPARKY 3, ARIA 1.2                 
REMARK 210   METHOD USED                   : SIMULATED ANNEALING                
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 50                                 
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 14                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR      
REMARK 210  SPECTROSCOPY.                                                       
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)               
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999                        
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996                     
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500 11 PHE A 176   CE1   PHE A 176   CZ      0.117                       
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASN A 117     -109.93    -88.49                                   
REMARK 500  1 GLU A 122      -98.58     62.47                                   
REMARK 500  1 ARG A 124     -152.58    -95.28                                   
REMARK 500  1 ILE A 126       67.60    -64.81                                   
REMARK 500  1 ALA A 129       21.38    -62.73                                   
REMARK 500  1 PRO A 131       34.39    -64.85                                   
REMARK 500  1 TRP A 145       -9.05    -57.11                                   
REMARK 500  1 LEU A 158        7.93    -63.54                                   
REMARK 500  1 MET A 171      131.08    -29.96                                   
REMARK 500  1 THR A 180       89.43   -157.07                                   
REMARK 500  1 SER A 182     -134.85     51.78                                   
REMARK 500  2 SER A 106       80.99     61.52                                   
REMARK 500  2 MET A 108      140.46     72.06                                   
REMARK 500  2 LYS A 111       42.86    -91.24                                   
REMARK 500  2 PRO A 116      100.03    -28.93                                   
REMARK 500  2 GLU A 122      -92.27    -60.81                                   
REMARK 500  2 ARG A 123      -40.29   -147.52                                   
REMARK 500  2 ILE A 126       66.15    -69.08                                   
REMARK 500  2 PRO A 131       26.89    -71.14                                   
REMARK 500  2 TRP A 145       -9.19    -56.57                                   
REMARK 500  2 LEU A 158        8.02    -58.38                                   
REMARK 500  2 LEU A 179      -30.48   -130.23                                   
REMARK 500  2 THR A 180       80.31   -153.55                                   
REMARK 500  2 PRO A 181     -156.92    -90.58                                   
REMARK 500  2 SER A 182      -91.49     68.77                                   
REMARK 500  3 SER A 106       77.19   -100.42                                   
REMARK 500  3 GLU A 110       34.34    -95.41                                   
REMARK 500  3 MET A 113       74.15   -115.52                                   
REMARK 500  3 THR A 119       18.49   -155.53                                   
REMARK 500  3 THR A 120      -75.43    -88.50                                   
REMARK 500  3 ASN A 121       47.32    -81.93                                   
REMARK 500  3 ARG A 124      -64.04   -148.83                                   
REMARK 500  3 PRO A 131       28.35    -75.82                                   
REMARK 500  3 ASP A 154       76.64   -152.02                                   
REMARK 500  3 LEU A 158        6.91    -64.42                                   
REMARK 500  3 THR A 180       95.49   -162.06                                   
REMARK 500  3 SER A 182     -178.37     61.58                                   
REMARK 500  3 TYR A 183      -66.81     68.87                                   
REMARK 500  4 MET A 108       -0.74   -154.23                                   
REMARK 500  4 GLU A 109      -77.15    -77.72                                   
REMARK 500  4 GLU A 110     -177.28     60.03                                   
REMARK 500  4 LYS A 111      -14.07     74.75                                   
REMARK 500  4 PRO A 116      103.90    -35.07                                   
REMARK 500  4 ASN A 121      115.14   -179.39                                   
REMARK 500  4 ARG A 123     -109.97     61.17                                   
REMARK 500  4 ARG A 124      -85.42    -61.90                                   
REMARK 500  4 VAL A 125      -37.65   -135.45                                   
REMARK 500  4 ILE A 126       50.94     27.69                                   
REMARK 500  4 PRO A 131       31.68    -73.64                                   
REMARK 500  4 PRO A 153       46.59    -92.02                                   
REMARK 500  4 ASP A 154       23.68    168.92                                   
REMARK 500  4 LEU A 158        6.43    -64.59                                   
REMARK 500  4 THR A 180       91.81   -165.16                                   
REMARK 500  4 PRO A 181     -169.10    -73.95                                   
REMARK 500  4 SER A 182     -158.37     65.06                                   
REMARK 500  5 PRO A 116     -152.07    -86.19                                   
REMARK 500  5 ASN A 117      -73.84   -113.04                                   
REMARK 500  5 MET A 118      -14.33   -174.79                                   
REMARK 500  5 THR A 119       64.73     73.49                                   
REMARK 500  5 THR A 120     -145.89   -119.40                                   
REMARK 500  5 GLU A 122       71.43     41.18                                   
REMARK 500  5 ARG A 123      -21.74     75.54                                   
REMARK 500  5 ARG A 124      -81.53    -68.13                                   
REMARK 500  5 PRO A 131       35.14    -69.28                                   
REMARK 500  5 ASP A 154       85.05   -160.81                                   
REMARK 500  5 LEU A 158        3.67    -67.14                                   
REMARK 500  5 THR A 180      111.62   -160.32                                   
REMARK 500  5 THR A 198       79.82   -117.33                                   
REMARK 500  6 HIS A 107       52.77   -162.24                                   
REMARK 500  6 MET A 108      -64.15   -105.58                                   
REMARK 500  6 LYS A 111      -66.76   -158.14                                   
REMARK 500  6 HIS A 112       33.74   -161.65                                   
REMARK 500  6 ASN A 117      -81.09    -87.02                                   
REMARK 500  6 THR A 119       32.06    -97.29                                   
REMARK 500  6 THR A 120      -75.53   -155.35                                   
REMARK 500  6 ASN A 121      129.50     68.37                                   
REMARK 500  6 PRO A 131       29.84    -70.33                                   
REMARK 500  6 TRP A 145       -9.14    -55.91                                   
REMARK 500  6 LEU A 158        6.02    -59.41                                   
REMARK 500  6 THR A 180       87.42   -161.64                                   
REMARK 500  6 PRO A 181      -78.66    -74.15                                   
REMARK 500  6 SER A 182      -86.89    -20.06                                   
REMARK 500  6 TYR A 183       12.55   -140.72                                   
REMARK 500  7 HIS A 107       52.77   -162.24                                   
REMARK 500  7 MET A 108      -64.15   -105.58                                   
REMARK 500  7 LYS A 111      -66.76   -158.14                                   
REMARK 500  7 HIS A 112       33.74   -161.65                                   
REMARK 500  7 ASN A 117      -81.09    -87.02                                   
REMARK 500  7 THR A 119       32.06    -97.29                                   
REMARK 500  7 THR A 120      -75.53   -155.35                                   
REMARK 500  7 ASN A 121      129.50     68.37                                   
REMARK 500  7 PRO A 131       29.84    -70.33                                   
REMARK 500  7 TRP A 145       -9.14    -55.91                                   
REMARK 500  7 LEU A 158        6.02    -59.41                                   
REMARK 500  7 THR A 180       87.42   -161.64                                   
REMARK 500  7 PRO A 181      -78.66    -74.15                                   
REMARK 500  7 SER A 182      -86.89    -20.06                                   
REMARK 500  7 TYR A 183       12.55   -140.72                                   
REMARK 500  8 SER A 106       89.64   -163.84                                   
REMARK 500  8 GLU A 110      -69.79     71.72                                   
REMARK 500  8 LYS A 111      141.82     68.81                                   
REMARK 500  8 ASN A 117      -64.75     72.85                                   
REMARK 500  8 ASN A 121      143.14   -173.52                                   
REMARK 500  8 GLU A 122      137.22   -174.92                                   
REMARK 500  8 ARG A 123      -85.26     61.52                                   
REMARK 500  8 ARG A 124     -157.71    -77.53                                   
REMARK 500  8 ILE A 126       43.17     28.98                                   
REMARK 500  8 TRP A 145       -9.97    -58.92                                   
REMARK 500  8 PRO A 153     -115.71    -89.96                                   
REMARK 500  8 ASP A 154       66.70    -61.13                                   
REMARK 500  8 LEU A 158        7.88    -61.15                                   
REMARK 500  8 THR A 180       87.29   -158.08                                   
REMARK 500  8 SER A 182      -85.60     72.29                                   
REMARK 500  8 ASN A 184      -35.45     83.70                                   
REMARK 500  9 SER A 106       46.39    -82.54                                   
REMARK 500  9 HIS A 112       73.00   -106.94                                   
REMARK 500  9 THR A 119       51.99    -91.86                                   
REMARK 500  9 THR A 120      -61.78   -120.37                                   
REMARK 500  9 ARG A 124      -78.50   -151.23                                   
REMARK 500  9 ALA A 129       19.62    -61.74                                   
REMARK 500  9 PRO A 131       27.64    -67.45                                   
REMARK 500  9 TRP A 145       -9.01    -56.79                                   
REMARK 500  9 LEU A 158        7.39    -63.90                                   
REMARK 500  9 THR A 180       93.87   -164.09                                   
REMARK 500  9 PRO A 181     -178.16    -60.39                                   
REMARK 500  9 SER A 182      107.22    -53.20                                   
REMARK 500  9 TYR A 183      -64.62    155.23                                   
REMARK 500  9 ILE A 187      -60.50    -93.46                                   
REMARK 500  9 THR A 198       79.02   -114.61                                   
REMARK 500 10 HIS A 107      -51.07   -140.50                                   
REMARK 500 10 HIS A 112       83.56    -66.37                                   
REMARK 500 10 ASN A 117      -81.42    -91.50                                   
REMARK 500 10 ARG A 124      -88.62   -132.81                                   
REMARK 500 10 PRO A 131       29.76    -68.78                                   
REMARK 500 10 TRP A 145       -9.62    -56.54                                   
REMARK 500 10 ASP A 154       78.68   -153.92                                   
REMARK 500 10 LEU A 158        4.94    -59.04                                   
REMARK 500 10 THR A 180       87.02   -163.41                                   
REMARK 500 10 SER A 182      -80.90     67.87                                   
REMARK 500 10 ASN A 184      -36.39     89.73                                   
REMARK 500 10 ILE A 187      -60.57    -91.36                                   
REMARK 500 11 PRO A 115      109.66    -39.49                                   
REMARK 500 11 ASN A 121     -152.48    174.65                                   
REMARK 500 11 GLU A 122      157.65     74.61                                   
REMARK 500 11 ARG A 123      -69.57     70.98                                   
REMARK 500 11 ILE A 126       75.37    -66.96                                   
REMARK 500 11 PRO A 131       27.70    -75.85                                   
REMARK 500 11 TRP A 145       -9.40    -56.07                                   
REMARK 500 11 LEU A 158        9.96    -62.96                                   
REMARK 500 11 THR A 180       97.25   -164.73                                   
REMARK 500 11 SER A 182      -86.01     59.10                                   
REMARK 500 11 ASN A 184      -40.07     83.55                                   
REMARK 500 11 ILE A 187      -66.42    -92.00                                   
REMARK 500 12 MET A 108      -53.64     70.40                                   
REMARK 500 12 HIS A 112       86.07    -56.61                                   
REMARK 500 12 MET A 113       53.85   -146.66                                   
REMARK 500 12 ASN A 117     -166.38   -107.19                                   
REMARK 500 12 MET A 118       94.69     48.79                                   
REMARK 500 12 ARG A 124      -67.36   -107.74                                   
REMARK 500 12 PRO A 131       34.83    -73.14                                   
REMARK 500 12 ASP A 154       87.70   -160.33                                   
REMARK 500 12 LEU A 158        7.73    -63.85                                   
REMARK 500 12 THR A 180       87.68   -160.11                                   
REMARK 500 12 SER A 182      -99.03     62.36                                   
REMARK 500 12 ASN A 184      -36.55     81.86                                   
REMARK 500 13 LYS A 111      136.75     72.05                                   
REMARK 500 13 HIS A 112       86.76    -69.13                                   
REMARK 500 13 ASN A 117       37.34    -98.08                                   
REMARK 500 13 GLU A 122     -142.31     48.91                                   
REMARK 500 13 ARG A 124      -91.47    -73.66                                   
REMARK 500 13 LEU A 158       11.44    -59.59                                   
REMARK 500 13 THR A 180      109.82   -162.01                                   
REMARK 500 13 PRO A 181     -176.43    -69.66                                   
REMARK 500 13 SER A 182       98.25    -55.08                                   
REMARK 500 13 TYR A 183      -67.88    172.05                                   
REMARK 500 13 THR A 198      -58.12   -121.22                                   
REMARK 500 13 PRO A 199      -79.36    -94.76                                   
REMARK 500 14 SER A 106      156.53     67.84                                   
REMARK 500 14 HIS A 112      -23.60     73.54                                   
REMARK 500 14 PRO A 116      107.36    -31.85                                   
REMARK 500 14 ASN A 121      -88.12     68.59                                   
REMARK 500 14 GLU A 122      141.01     65.11                                   
REMARK 500 14 ARG A 124      -75.56   -141.65                                   
REMARK 500 14 ILE A 126       73.68     55.33                                   
REMARK 500 14 ALA A 129       24.76    -61.19                                   
REMARK 500 14 PRO A 131       27.65    -61.74                                   
REMARK 500 14 TRP A 145       -8.89    -56.16                                   
REMARK 500 14 LEU A 158        8.42    -64.92                                   
REMARK 500 14 THR A 180      102.28   -161.48                                   
REMARK 500 14 PRO A 181      175.19    -58.97                                   
REMARK 500 14 SER A 182      100.86    -57.10                                   
REMARK 500 14 TYR A 183      -58.79    169.93                                   
REMARK 500 14 ILE A 187      -62.60    -95.58                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500  4 TYR A 150         0.05    SIDE_CHAIN                              
REMARK 500 10 TYR A 150         0.05    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1SXE A  108   201  UNP    P11308   ERG_HUMAN      115    208             
SEQADV 1SXE GLY A  105  UNP  P11308              CLONING ARTIFACT               
SEQADV 1SXE SER A  106  UNP  P11308              CLONING ARTIFACT               
SEQADV 1SXE HIS A  107  UNP  P11308              CLONING ARTIFACT               
SEQRES   1 A   97  GLY SER HIS MET GLU GLU LYS HIS MET PRO PRO PRO ASN          
SEQRES   2 A   97  MET THR THR ASN GLU ARG ARG VAL ILE VAL PRO ALA ASP          
SEQRES   3 A   97  PRO THR LEU TRP SER THR ASP HIS VAL ARG GLN TRP LEU          
SEQRES   4 A   97  GLU TRP ALA VAL LYS GLU TYR GLY LEU PRO ASP VAL ASN          
SEQRES   5 A   97  ILE LEU LEU PHE GLN ASN ILE ASP GLY LYS GLU LEU CYS          
SEQRES   6 A   97  LYS MET THR LYS ASP ASP PHE GLN ARG LEU THR PRO SER          
SEQRES   7 A   97  TYR ASN ALA ASP ILE LEU LEU SER HIS LEU HIS TYR LEU          
SEQRES   8 A   97  ARG GLU THR PRO LEU PRO                                      
HELIX    1   1 MET A  108  MET A  113  5                                   6    
HELIX    2   2 SER A  135  GLY A  151  1                                  17    
HELIX    3   3 ASN A  156  PHE A  160  5                                   5    
HELIX    4   4 ASP A  164  CYS A  169  1                                   6    
HELIX    5   5 THR A  172  GLN A  177  1                                   6    
HELIX    6   6 PRO A  181  ARG A  196  1                                  16    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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