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Database: PDB
Entry: 1TOZ
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Original site: 1TOZ 
HEADER    SIGNALING PROTEIN                       15-JUN-04   1TOZ              
TITLE     NMR STRUCTURE OF THE HUMAN NOTCH-1 LIGAND BINDING REGION              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1;                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: NOTCH-1 EGF 11-13;                                         
COMPND   5 SYNONYM: NOTCH 1, HN1, TRANSLOCATION-ASSOCIATED NOTCH                
COMPND   6 PROTEIN TAN-1;                                                       
COMPND   7 ENGINEERED: YES;                                                     
COMPND   8 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;                            
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 511693;                                     
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21;                                      
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PQE30                                     
KEYWDS    NOTCH, EGF, CALCIUM BINDING, LIGAND BINDING, MODULE,                  
KEYWDS   2 SIGNALING PROTEIN                                                    
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    S.HAMBLETON,N.Y.VALEYEV,A.MURANYI,V.KNOTT,J.M.WERNER,                 
AUTHOR   2 A.J.MCMICHAEL,P.A.HANDFORD,A.K.DOWNING                               
REVDAT   3   24-FEB-09 1TOZ    1       VERSN                                    
REVDAT   2   28-DEC-04 1TOZ    1       JRNL                                     
REVDAT   1   12-OCT-04 1TOZ    0                                                
JRNL        AUTH   S.HAMBLETON,N.V.VALEYEV,A.MURANYI,V.KNOTT,                   
JRNL        AUTH 2 J.M.WERNER,A.J.MCMICHAEL,P.A.HANDFORD,A.K.DOWNING            
JRNL        TITL   STRUCTURAL AND FUNCTIONAL PROPERTIES OF THE HUMAN            
JRNL        TITL 2 NOTCH-1 LIGAND BINDING REGION                                
JRNL        REF    STRUCTURE                     V.  12  2173 2004              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   15576031                                                     
JRNL        DOI    10.1016/J.STR.2004.09.012                                    
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   A.MURANYI,S.HAMBLETON,V.KNOTT,A.MCMICHAEL,                   
REMARK   1  AUTH 2 P.A.HANDFORD,A.K.DOWNING                                     
REMARK   1  TITL   1H, 13C, AND 15N RESONANCE ASSIGNMENTS OF HUMAN              
REMARK   1  TITL 2 NOTCH-1 CALCIUM BINDING EGF DOMAINS 11-13                    
REMARK   1  REF    J.BIOMOL.NMR                  V.  29   443 2004              
REMARK   1  REFN                   ISSN 0925-2738                               
REMARK   1  PMID   15213460                                                     
REMARK   1  DOI    10.1023/B:JNMR.0000032521.42723.1A                           
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 1.0.6                                          
REMARK   3   AUTHORS     : GUNTERT, P.                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: INPUT MANUALLY ASSIGNED 15N-EDITED        
REMARK   3  NOESY PEAKS, UNASSIGNED 13C-EDITED NOESY PEAKS. SEE                 
REMARK   3  PUBLICATION FOR FURTHER DETAILS.                                    
REMARK   4                                                                      
REMARK   4 1TOZ COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 17-JUN-04.                  
REMARK 100 THE RCSB ID CODE IS RCSB022805.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 6.1                                
REMARK 210  IONIC STRENGTH                 : 15MM CACL2                         
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 0.5-1MM PROTEIN                    
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY, HMQC-J        
REMARK 210  SPECTROMETER FIELD STRENGTH    : 600 MHZ, 750 MHZ, 800 MHZ          
REMARK 210  SPECTROMETER MODEL             : OMEGA, DRX                         
REMARK 210  SPECTROMETER MANUFACTURER      : GE, BRUKER                         
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : CYANA 1.0.6                        
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 600                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY                             
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 9                   
REMARK 210                                                                      
REMARK 210 REMARK: ADDITIONAL TORSION ANGLE RESTRAINTS DERIVED USING            
REMARK 210  TALOS. CALCIUM ATOMS INCORPORATED DURING STRUCTURE                  
REMARK 210  CALCULATIONS, AND RESTRAINTS INCORPORATED BASED ON THE CRYSTAL      
REMARK 210  STRUCTURE 1EDM AS DESCRIBED.                                        
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI           DISTANCE          
REMARK 500   N    THR A   445     O    ILE A   451              2.19            
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 LEU A 418      -92.94    -73.39                                   
REMARK 500  1 GLU A 424     -106.94     68.61                                   
REMARK 500  1 ALA A 426       90.04    168.08                                   
REMARK 500  1 GLN A 442      -93.80     68.68                                   
REMARK 500  1 THR A 445     -126.64   -101.93                                   
REMARK 500  1 CYS A 449       62.87     87.61                                   
REMARK 500  1 GLU A 450       15.04   -149.39                                   
REMARK 500  1 ASN A 459       56.55   -144.28                                   
REMARK 500  1 GLN A 462     -145.91     86.80                                   
REMARK 500  1 ASP A 464       38.12   -151.19                                   
REMARK 500  1 THR A 491      176.44     48.53                                   
REMARK 500  1 GLU A 493       22.65   -146.20                                   
REMARK 500  1 SER A 497       90.57   -166.50                                   
REMARK 500  1 CYS A 499      -48.67     77.52                                   
REMARK 500  1 LEU A 500      170.90     69.66                                   
REMARK 500  1 HIS A 501     -110.96     67.39                                   
REMARK 500  1 ASN A 510      -48.06    174.84                                   
REMARK 500  1 PRO A 517     -162.95    -75.05                                   
REMARK 500  1 THR A 518      -46.15   -168.28                                   
REMARK 500  1 PHE A 520      -64.38    -95.95                                   
REMARK 500  1 HIS A 523      -79.28   -115.15                                   
REMARK 500  1 LEU A 524      -48.16   -171.55                                   
REMARK 500  1 CYS A 525       97.49     63.97                                   
REMARK 500  2 LEU A 418      -90.07    -73.03                                   
REMARK 500  2 GLU A 424      -99.40     69.50                                   
REMARK 500  2 ALA A 426       97.03    169.47                                   
REMARK 500  2 GLN A 442     -100.01     65.49                                   
REMARK 500  2 THR A 445     -123.90   -103.91                                   
REMARK 500  2 CYS A 449       68.40     97.01                                   
REMARK 500  2 GLU A 450       23.41   -161.42                                   
REMARK 500  2 ASN A 459       51.49   -141.08                                   
REMARK 500  2 GLN A 462     -150.20     86.06                                   
REMARK 500  2 ALA A 465      125.93   -179.54                                   
REMARK 500  2 CYS A 476       86.97   -154.86                                   
REMARK 500  2 THR A 491      161.48    -40.67                                   
REMARK 500  2 GLU A 493       25.34   -158.08                                   
REMARK 500  2 SER A 497       70.68    159.62                                   
REMARK 500  2 CYS A 499      103.13    -47.32                                   
REMARK 500  2 LEU A 500       22.62   -141.64                                   
REMARK 500  2 ASN A 510      -56.70    177.19                                   
REMARK 500  2 GLU A 515     -163.29    -77.11                                   
REMARK 500  2 PRO A 517     -162.31    -75.00                                   
REMARK 500  2 THR A 518      -41.45   -165.92                                   
REMARK 500  2 HIS A 523       72.35   -156.52                                   
REMARK 500  2 CYS A 525      -71.65   -175.61                                   
REMARK 500  3 LEU A 418      -78.22    -65.67                                   
REMARK 500  3 CYS A 423      -19.76    -48.83                                   
REMARK 500  3 GLU A 424     -100.95     69.15                                   
REMARK 500  3 ALA A 426       94.64   -178.09                                   
REMARK 500  3 GLN A 442      -98.86     52.15                                   
REMARK 500  3 THR A 445     -124.93   -101.30                                   
REMARK 500  3 CYS A 449       56.20     88.10                                   
REMARK 500  3 ASN A 459       48.33   -142.32                                   
REMARK 500  3 GLN A 462     -176.29     80.47                                   
REMARK 500  3 ALA A 465      113.39    164.29                                   
REMARK 500  3 CYS A 476      106.21   -178.18                                   
REMARK 500  3 ASN A 490       90.19    -64.13                                   
REMARK 500  3 THR A 491      166.76     51.95                                   
REMARK 500  3 GLU A 493       38.03   -156.57                                   
REMARK 500  3 CYS A 494       49.36   -166.78                                   
REMARK 500  3 CYS A 499      -54.00     70.86                                   
REMARK 500  3 LEU A 500     -157.07     79.40                                   
REMARK 500  3 ASN A 510      -40.41   -173.63                                   
REMARK 500  3 GLU A 511     -167.61   -162.13                                   
REMARK 500  3 GLU A 515      170.00    -44.95                                   
REMARK 500  3 THR A 518      -43.71   -132.86                                   
REMARK 500  3 LEU A 524      169.55    174.41                                   
REMARK 500  4 LEU A 418      -85.87    -72.16                                   
REMARK 500  4 GLU A 424      -71.81     77.39                                   
REMARK 500  4 HIS A 425      -42.84   -155.32                                   
REMARK 500  4 GLN A 442      -95.12     42.42                                   
REMARK 500  4 THR A 445     -122.66   -102.60                                   
REMARK 500  4 CYS A 449       61.34     85.87                                   
REMARK 500  4 GLU A 450       21.42   -154.33                                   
REMARK 500  4 ASN A 459       58.98   -146.30                                   
REMARK 500  4 GLN A 462     -157.29     78.19                                   
REMARK 500  4 LEU A 468       80.31   -154.95                                   
REMARK 500  4 THR A 491      160.67    -39.42                                   
REMARK 500  4 GLU A 493       30.83   -146.54                                   
REMARK 500  4 SER A 497       85.42   -179.03                                   
REMARK 500  4 LEU A 500     -156.12     46.28                                   
REMARK 500  4 ASN A 510      -35.90    172.42                                   
REMARK 500  4 THR A 518      -63.12   -162.80                                   
REMARK 500  4 THR A 521       77.86     48.96                                   
REMARK 500  4 HIS A 523      122.82     79.04                                   
REMARK 500  5 LEU A 418      -89.45    -73.32                                   
REMARK 500  5 GLU A 424      -88.72     72.38                                   
REMARK 500  5 HIS A 425       34.90   -144.84                                   
REMARK 500  5 ALA A 426      101.01    179.41                                   
REMARK 500  5 GLN A 442      -98.11     54.50                                   
REMARK 500  5 THR A 445     -117.83   -104.06                                   
REMARK 500  5 CYS A 449       71.14     96.62                                   
REMARK 500  5 GLU A 450       26.67   -166.16                                   
REMARK 500  5 ASN A 459       57.99   -145.73                                   
REMARK 500  5 GLN A 462     -147.92     43.39                                   
REMARK 500  5 ASN A 463       41.04    -99.10                                   
REMARK 500  5 ALA A 465      145.35    179.20                                   
REMARK 500  5 LEU A 468       88.78   -160.90                                   
REMARK 500  5 GLN A 470     -169.15   -125.86                                   
REMARK 500  5 THR A 491      157.69    -38.46                                   
REMARK 500  5 SER A 497       91.56     63.60                                   
REMARK 500  5 HIS A 501      -58.57     69.76                                   
REMARK 500  5 GLU A 515     -176.32    -66.63                                   
REMARK 500  5 THR A 521       99.15     48.39                                   
REMARK 500  5 HIS A 523     -179.86    179.24                                   
REMARK 500  5 CYS A 525      -64.22    177.19                                   
REMARK 500  6 LEU A 418      -77.48    -46.84                                   
REMARK 500  6 GLU A 424      -66.93     84.05                                   
REMARK 500  6 HIS A 425      -41.70   -161.03                                   
REMARK 500  6 GLN A 442      -94.58     69.29                                   
REMARK 500  6 THR A 445     -121.58   -102.47                                   
REMARK 500  6 CYS A 449       71.38    100.27                                   
REMARK 500  6 GLU A 450       21.63   -163.03                                   
REMARK 500  6 ASN A 459       49.96   -140.59                                   
REMARK 500  6 CYS A 461       21.93     80.17                                   
REMARK 500  6 GLN A 462     -112.46     69.03                                   
REMARK 500  6 ALA A 465      149.69    169.83                                   
REMARK 500  6 THR A 491      168.05     53.59                                   
REMARK 500  6 GLU A 493       30.67   -148.82                                   
REMARK 500  6 CYS A 494       31.03   -140.47                                   
REMARK 500  6 CYS A 499      -54.71     70.98                                   
REMARK 500  6 LEU A 500     -157.72     78.96                                   
REMARK 500  6 ASN A 510      -40.92    163.98                                   
REMARK 500  6 PRO A 517     -162.23    -74.95                                   
REMARK 500  6 THR A 518       52.25   -174.35                                   
REMARK 500  6 PHE A 520       99.54    -39.15                                   
REMARK 500  6 HIS A 523       97.31    168.95                                   
REMARK 500  6 LEU A 524       48.66   -165.28                                   
REMARK 500  7 LEU A 418      -81.60    -68.75                                   
REMARK 500  7 GLU A 424      -75.47     77.59                                   
REMARK 500  7 HIS A 425      -43.55   -143.04                                   
REMARK 500  7 GLN A 442      -98.02     67.26                                   
REMARK 500  7 THR A 445     -121.25   -103.32                                   
REMARK 500  7 CYS A 449       60.77     92.76                                   
REMARK 500  7 GLU A 450       24.63   -158.08                                   
REMARK 500  7 ASN A 459       50.38   -141.51                                   
REMARK 500  7 GLN A 462     -173.16     78.12                                   
REMARK 500  7 ALA A 465      131.58    163.35                                   
REMARK 500  7 THR A 491     -169.80     40.36                                   
REMARK 500  7 SER A 496      -63.11    -95.31                                   
REMARK 500  7 CYS A 499       58.91    -68.33                                   
REMARK 500  7 HIS A 501      -83.55    157.14                                   
REMARK 500  7 ILE A 509      108.02    -48.39                                   
REMARK 500  7 GLU A 511      137.55    164.90                                   
REMARK 500  7 GLU A 515     -147.60    -68.09                                   
REMARK 500  7 PRO A 517     -162.01    -74.98                                   
REMARK 500  7 THR A 518      -42.15   -158.55                                   
REMARK 500  7 THR A 521       79.26     36.60                                   
REMARK 500  7 HIS A 523      115.32    -39.81                                   
REMARK 500  7 LEU A 524      -75.10   -155.76                                   
REMARK 500  8 GLU A 424      -72.54     85.89                                   
REMARK 500  8 HIS A 425      -40.96   -159.98                                   
REMARK 500  8 GLN A 442      -93.24     69.95                                   
REMARK 500  8 THR A 445     -130.35   -104.58                                   
REMARK 500  8 CYS A 449       59.86     82.71                                   
REMARK 500  8 GLU A 450       12.46   -146.31                                   
REMARK 500  8 ASN A 459       50.27   -140.50                                   
REMARK 500  8 GLN A 462     -156.99     78.91                                   
REMARK 500  8 ASP A 464       45.74    -90.79                                   
REMARK 500  8 ALA A 465      124.69   -179.31                                   
REMARK 500  8 ASN A 490       91.60    -67.95                                   
REMARK 500  8 THR A 491      168.28     52.87                                   
REMARK 500  8 GLU A 493       28.66   -143.46                                   
REMARK 500  8 SER A 497       88.69     38.57                                   
REMARK 500  8 HIS A 501      -59.34     69.85                                   
REMARK 500  8 ASN A 510      -33.50    170.87                                   
REMARK 500  8 GLU A 511     -165.90   -171.22                                   
REMARK 500  8 GLU A 515      165.18    -48.95                                   
REMARK 500  8 PRO A 517     -162.16    -74.95                                   
REMARK 500  8 THR A 518       47.42   -157.25                                   
REMARK 500  8 THR A 521      167.20     53.05                                   
REMARK 500  8 LEU A 524      130.57     70.72                                   
REMARK 500  8 CYS A 525      -64.41   -133.31                                   
REMARK 500  9 LEU A 418      -77.70    -53.52                                   
REMARK 500  9 CYS A 423      -19.98    -49.51                                   
REMARK 500  9 GLU A 424     -108.73     68.38                                   
REMARK 500  9 GLN A 442     -103.21     54.83                                   
REMARK 500  9 THR A 445     -124.18   -103.06                                   
REMARK 500  9 CYS A 449       57.40     93.33                                   
REMARK 500  9 GLU A 450       12.10   -148.86                                   
REMARK 500  9 ASN A 459       58.76   -149.26                                   
REMARK 500  9 GLN A 462     -171.30     83.21                                   
REMARK 500  9 ALA A 465      128.68   -179.79                                   
REMARK 500  9 CYS A 494       54.31   -102.73                                   
REMARK 500  9 SER A 496     -138.11   -156.72                                   
REMARK 500  9 SER A 497       74.76     35.31                                   
REMARK 500  9 HIS A 501      -42.58     82.60                                   
REMARK 500  9 ILE A 509       79.83    -63.43                                   
REMARK 500  9 ASN A 510      -39.89    157.29                                   
REMARK 500  9 PRO A 517     -163.89    -74.86                                   
REMARK 500  9 THR A 518       53.34   -175.58                                   
REMARK 500  9 PHE A 520      100.74    -57.21                                   
REMARK 500  9 HIS A 523      171.13    178.44                                   
REMARK 500 10 LEU A 418      -84.06    -76.11                                   
REMARK 500 10 GLU A 424      -63.26     85.34                                   
REMARK 500 10 HIS A 425      -41.64   -161.13                                   
REMARK 500 10 GLN A 442     -100.00     53.85                                   
REMARK 500 10 THR A 445     -122.34   -102.45                                   
REMARK 500 10 CYS A 449       56.95     90.73                                   
REMARK 500 10 GLU A 450       16.78   -149.46                                   
REMARK 500 10 CYS A 461        1.62     80.36                                   
REMARK 500 10 GLN A 462     -162.24     34.29                                   
REMARK 500 10 ASN A 463      -40.17     79.56                                   
REMARK 500 10 ASP A 464      -19.83    150.75                                   
REMARK 500 10 SER A 497       70.21   -167.24                                   
REMARK 500 10 CYS A 499      -77.92     67.65                                   
REMARK 500 10 LEU A 500      141.28     61.58                                   
REMARK 500 10 HIS A 501      -58.78     69.36                                   
REMARK 500 10 ASN A 510      -39.36    160.90                                   
REMARK 500 10 GLU A 515      176.28    -51.43                                   
REMARK 500 10 THR A 518      -40.17   -165.91                                   
REMARK 500 10 HIS A 523      168.61     55.27                                   
REMARK 500 11 GLU A 424     -100.31     65.42                                   
REMARK 500 11 GLN A 442      -96.09     60.51                                   
REMARK 500 11 THR A 445     -117.23   -105.88                                   
REMARK 500 11 CYS A 449       68.92     96.57                                   
REMARK 500 11 GLU A 450       27.43   -166.19                                   
REMARK 500 11 ASN A 459       52.59   -140.91                                   
REMARK 500 11 GLN A 462     -167.15     81.95                                   
REMARK 500 11 ALA A 465      130.85    173.07                                   
REMARK 500 11 CYS A 487       28.17     43.37                                   
REMARK 500 11 ASN A 490       90.69    -64.44                                   
REMARK 500 11 THR A 491      165.75     52.63                                   
REMARK 500 11 SER A 496      -75.82    -68.76                                   
REMARK 500 11 CYS A 499       57.48     39.97                                   
REMARK 500 11 HIS A 501      -59.37     69.39                                   
REMARK 500 11 ASN A 510      -35.40    171.01                                   
REMARK 500 11 GLU A 511     -161.94   -163.64                                   
REMARK 500 11 GLU A 515     -171.44    -54.12                                   
REMARK 500 11 PRO A 517     -163.59    -75.02                                   
REMARK 500 11 THR A 518       63.71   -167.40                                   
REMARK 500 11 THR A 521       81.74     38.41                                   
REMARK 500 11 HIS A 523       83.13   -167.18                                   
REMARK 500 11 LEU A 524      141.96   -171.25                                   
REMARK 500 12 LEU A 418      -90.60    -73.86                                   
REMARK 500 12 GLU A 424      -70.79     76.01                                   
REMARK 500 12 HIS A 425      -44.36   -151.95                                   
REMARK 500 12 GLN A 442      -93.64     68.48                                   
REMARK 500 12 THR A 445     -123.28   -101.17                                   
REMARK 500 12 CYS A 449       68.80     92.82                                   
REMARK 500 12 GLU A 450       17.73   -157.23                                   
REMARK 500 12 ASN A 459       60.77   -154.99                                   
REMARK 500 12 GLN A 462     -147.79     47.90                                   
REMARK 500 12 ASN A 463       42.55   -102.34                                   
REMARK 500 12 ASP A 464       42.22   -145.45                                   
REMARK 500 12 ALA A 465      129.12    168.18                                   
REMARK 500 12 LEU A 468       87.16   -159.60                                   
REMARK 500 12 CYS A 487       23.67     49.50                                   
REMARK 500 12 THR A 491      157.77    -37.55                                   
REMARK 500 12 GLU A 493       20.09   -149.38                                   
REMARK 500 12 CYS A 499       74.56     41.69                                   
REMARK 500 12 HIS A 501      -59.62     69.14                                   
REMARK 500 12 ILE A 509       62.81    -69.81                                   
REMARK 500 12 ASN A 510      -63.56    170.99                                   
REMARK 500 12 GLU A 515     -150.51    -90.84                                   
REMARK 500 12 THR A 518       37.17   -157.30                                   
REMARK 500 12 PHE A 520       16.66   -145.35                                   
REMARK 500 12 LEU A 524      152.70     70.64                                   
REMARK 500 13 LEU A 418      -70.91    -65.33                                   
REMARK 500 13 CYS A 423      -18.04    -49.72                                   
REMARK 500 13 GLU A 424      -63.45     73.90                                   
REMARK 500 13 HIS A 425      -42.21   -166.32                                   
REMARK 500 13 GLN A 442      -99.31     66.97                                   
REMARK 500 13 THR A 445     -123.51   -104.14                                   
REMARK 500 13 CYS A 449       61.16     95.84                                   
REMARK 500 13 GLU A 450       21.68   -156.31                                   
REMARK 500 13 GLN A 462     -164.65     34.61                                   
REMARK 500 13 ASN A 463      -44.30     77.36                                   
REMARK 500 13 ASP A 464      -18.87    152.06                                   
REMARK 500 13 ALA A 465      147.31    -38.39                                   
REMARK 500 13 ASN A 490       91.11    -64.49                                   
REMARK 500 13 THR A 491      174.24     50.16                                   
REMARK 500 13 CYS A 499       62.43     39.61                                   
REMARK 500 13 HIS A 501      -59.40     68.63                                   
REMARK 500 13 GLU A 511     -152.51   -166.32                                   
REMARK 500 13 GLU A 515      171.99    -53.69                                   
REMARK 500 13 PRO A 517     -166.89    -74.95                                   
REMARK 500 13 THR A 518      -44.51   -159.97                                   
REMARK 500 13 PHE A 520       88.35    -65.08                                   
REMARK 500 13 THR A 521       83.00    -60.32                                   
REMARK 500 14 LEU A 418      -79.12    -65.00                                   
REMARK 500 14 CYS A 423       47.17    -73.85                                   
REMARK 500 14 GLU A 424     -107.61     27.24                                   
REMARK 500 14 GLN A 442      -93.76     68.14                                   
REMARK 500 14 THR A 445     -122.82   -103.35                                   
REMARK 500 14 CYS A 449       65.27     98.43                                   
REMARK 500 14 GLU A 450       19.97   -156.84                                   
REMARK 500 14 ASN A 463      -20.45    148.34                                   
REMARK 500 14 ASP A 464      -20.16    152.14                                   
REMARK 500 14 LEU A 468       86.39   -160.99                                   
REMARK 500 14 THR A 491      154.47    -39.43                                   
REMARK 500 14 CYS A 499       75.01     48.42                                   
REMARK 500 14 HIS A 501      -45.42     81.19                                   
REMARK 500 14 ASN A 510      -42.65    156.42                                   
REMARK 500 14 THR A 518       44.67   -152.35                                   
REMARK 500 15 LEU A 418      -81.55    -68.41                                   
REMARK 500 15 GLU A 424      -90.48     66.81                                   
REMARK 500 15 GLN A 442      -95.05     67.60                                   
REMARK 500 15 THR A 445     -120.37   -102.56                                   
REMARK 500 15 CYS A 449       69.87     99.05                                   
REMARK 500 15 GLU A 450       23.19   -162.48                                   
REMARK 500 15 ASN A 459       50.76   -140.87                                   
REMARK 500 15 GLN A 462     -149.66     87.83                                   
REMARK 500 15 ALA A 465      137.75    170.99                                   
REMARK 500 15 THR A 491      159.59    -40.36                                   
REMARK 500 15 GLU A 493       13.20   -141.01                                   
REMARK 500 15 CYS A 499       94.77    -64.18                                   
REMARK 500 15 HIS A 501      -56.47     69.53                                   
REMARK 500 15 ASN A 510      -44.27    159.26                                   
REMARK 500 15 GLU A 515     -161.44    -70.72                                   
REMARK 500 15 PRO A 517     -162.23    -74.90                                   
REMARK 500 15 THR A 518      -42.20   -135.25                                   
REMARK 500 15 PHE A 520       42.08    -86.73                                   
REMARK 500 15 THR A 521      -43.61   -177.15                                   
REMARK 500 15 HIS A 523       51.98    167.27                                   
REMARK 500 15 LEU A 524      168.55     58.69                                   
REMARK 500 16 LEU A 418      -72.01    -64.81                                   
REMARK 500 16 CYS A 423      -19.11    -48.43                                   
REMARK 500 16 GLU A 424      -57.31     72.91                                   
REMARK 500 16 HIS A 425      -42.35   -162.54                                   
REMARK 500 16 GLN A 442      -95.52     70.12                                   
REMARK 500 16 THR A 445     -125.87   -102.89                                   
REMARK 500 16 CYS A 449       65.21     85.69                                   
REMARK 500 16 GLU A 450       19.10   -153.77                                   
REMARK 500 16 GLN A 462     -157.48     34.05                                   
REMARK 500 16 ASN A 463       30.15     72.09                                   
REMARK 500 16 CYS A 487       27.45     43.51                                   
REMARK 500 16 THR A 491      179.28    -49.26                                   
REMARK 500 16 GLU A 493       18.83   -148.35                                   
REMARK 500 16 LEU A 500     -176.09     46.65                                   
REMARK 500 16 ASN A 510      -30.29    163.38                                   
REMARK 500 16 GLU A 511     -170.64   -171.17                                   
REMARK 500 16 PRO A 517     -162.40    -75.10                                   
REMARK 500 16 THR A 518      -37.69   -173.28                                   
REMARK 500 16 THR A 521      -40.35   -171.48                                   
REMARK 500 16 HIS A 523      111.68     76.82                                   
REMARK 500 16 LEU A 524       84.71   -179.39                                   
REMARK 500 16 CYS A 525      104.63    -49.78                                   
REMARK 500 17 LEU A 418      -81.00    -61.04                                   
REMARK 500 17 GLU A 424      -93.23     69.67                                   
REMARK 500 17 HIS A 425       50.63   -140.38                                   
REMARK 500 17 ALA A 426       98.44    161.92                                   
REMARK 500 17 GLN A 442     -101.79     61.30                                   
REMARK 500 17 THR A 445     -123.63   -102.09                                   
REMARK 500 17 CYS A 449       58.24     90.34                                   
REMARK 500 17 GLU A 450       14.49   -148.99                                   
REMARK 500 17 GLU A 455       -9.54    -59.83                                   
REMARK 500 17 ASN A 459       57.23   -155.42                                   
REMARK 500 17 GLN A 462     -149.01     84.53                                   
REMARK 500 17 GLN A 470     -168.68   -112.16                                   
REMARK 500 17 ASN A 490       91.47    -67.75                                   
REMARK 500 17 THR A 491      154.87     57.23                                   
REMARK 500 17 CYS A 499       59.77     38.49                                   
REMARK 500 17 LEU A 500     -158.74    -57.02                                   
REMARK 500 17 HIS A 501     -107.44     67.92                                   
REMARK 500 17 ASN A 510      -36.90    176.64                                   
REMARK 500 17 GLU A 511     -163.42   -163.88                                   
REMARK 500 17 PRO A 517     -163.87    -75.04                                   
REMARK 500 17 THR A 518       50.90   -176.26                                   
REMARK 500 17 THR A 521       60.67   -102.85                                   
REMARK 500 18 LEU A 418      -81.93    -69.85                                   
REMARK 500 18 HIS A 425       -7.48     77.65                                   
REMARK 500 18 ALA A 426       75.21   -109.69                                   
REMARK 500 18 GLN A 442      -98.38     47.21                                   
REMARK 500 18 THR A 445     -114.66   -104.06                                   
REMARK 500 18 CYS A 449       60.63     91.88                                   
REMARK 500 18 GLU A 450       21.49   -156.90                                   
REMARK 500 18 ASN A 459       58.70   -156.31                                   
REMARK 500 18 GLN A 462     -169.99     74.37                                   
REMARK 500 18 ASN A 463       39.77    -92.31                                   
REMARK 500 18 ASP A 464       27.46   -142.28                                   
REMARK 500 18 GLN A 470     -168.04   -116.70                                   
REMARK 500 18 THR A 491      169.94     51.00                                   
REMARK 500 18 GLU A 493       26.63   -144.83                                   
REMARK 500 18 HIS A 501      -58.18     69.27                                   
REMARK 500 18 ASN A 510      -49.47    176.38                                   
REMARK 500 18 GLU A 511     -159.19   -153.63                                   
REMARK 500 18 THR A 521     -173.35     48.20                                   
REMARK 500 18 HIS A 523      115.24     64.67                                   
REMARK 500 19 LEU A 418      -79.44    -73.60                                   
REMARK 500 19 GLU A 424      -80.25     72.24                                   
REMARK 500 19 HIS A 425      -44.20   -139.75                                   
REMARK 500 19 GLN A 442     -101.33     44.16                                   
REMARK 500 19 THR A 445     -124.40   -102.62                                   
REMARK 500 19 CYS A 449       55.49     88.08                                   
REMARK 500 19 ASN A 459       59.28   -140.30                                   
REMARK 500 19 GLN A 462     -141.04     84.34                                   
REMARK 500 19 ASP A 464       54.12   -116.60                                   
REMARK 500 19 ALA A 465      120.34    176.25                                   
REMARK 500 19 LEU A 468       90.51   -170.09                                   
REMARK 500 19 THR A 491      178.26    -51.70                                   
REMARK 500 19 GLU A 493       43.32   -150.35                                   
REMARK 500 19 CYS A 494       46.11   -140.91                                   
REMARK 500 19 CYS A 499       93.88     46.66                                   
REMARK 500 19 LEU A 500       27.35   -145.37                                   
REMARK 500 19 HIS A 501      -46.05   -131.96                                   
REMARK 500 19 CYS A 505      106.11    -59.72                                   
REMARK 500 19 ILE A 509       64.00    -69.80                                   
REMARK 500 19 ASN A 510      -44.12    167.85                                   
REMARK 500 19 GLU A 515     -139.45    -61.27                                   
REMARK 500 19 THR A 518       51.79   -160.84                                   
REMARK 500 19 HIS A 523       85.70     55.05                                   
REMARK 500 19 LEU A 524       82.18   -165.80                                   
REMARK 500 19 CYS A 525       54.34   -175.95                                   
REMARK 500 20 LEU A 418      -78.56    -67.34                                   
REMARK 500 20 CYS A 423       56.97    -65.41                                   
REMARK 500 20 GLU A 424      -96.49     16.36                                   
REMARK 500 20 GLN A 442      -92.58     69.21                                   
REMARK 500 20 THR A 445     -121.53   -102.97                                   
REMARK 500 20 CYS A 449       62.46     95.48                                   
REMARK 500 20 GLU A 450       22.05   -157.37                                   
REMARK 500 20 ASN A 459       58.33   -150.95                                   
REMARK 500 20 GLN A 462     -155.02     82.85                                   
REMARK 500 20 LEU A 468       79.53   -154.73                                   
REMARK 500 20 THR A 491     -174.96    -49.55                                   
REMARK 500 20 CYS A 494       45.72   -165.19                                   
REMARK 500 20 CYS A 499       30.77     70.06                                   
REMARK 500 20 HIS A 501      -45.49     81.38                                   
REMARK 500 20 ASN A 510      -26.10    158.48                                   
REMARK 500 20 GLU A 511     -174.35   -175.73                                   
REMARK 500 20 CYS A 514       80.27    -69.48                                   
REMARK 500 20 GLU A 515      168.83    -44.99                                   
REMARK 500 20 THR A 518       43.73   -150.54                                   
REMARK 500 20 THR A 521      166.44     75.94                                   
REMARK 500 20 HIS A 523     -173.25    -62.35                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1EMO   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF FIBRILLIN CALCIUM BINDING EGF DOMAINS 32-33             
REMARK 900 RELATED ID: 1LMJ   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF FIBRILLIN CALCIUM BINDING EGF DOMAINS 12-13             
REMARK 900 RELATED ID: 1HJ7   RELATED DB: PDB                                   
REMARK 900 STRUCTURE OF LDL-RECEPTOR CALCIUM BINDING EGF DOMAINS A-B            
DBREF  1TOZ A  411   526  UNP    P46531   NOTC1_HUMAN    411    526             
SEQADV 1TOZ ILE A  477  UNP  P46531    MET   477 ENGINEERED                     
SEQRES   1 A  116  GLN ASP VAL ASP GLU CYS SER LEU GLY ALA ASN PRO CYS          
SEQRES   2 A  116  GLU HIS ALA GLY LYS CYS ILE ASN THR LEU GLY SER PHE          
SEQRES   3 A  116  GLU CYS GLN CYS LEU GLN GLY TYR THR GLY PRO ARG CYS          
SEQRES   4 A  116  GLU ILE ASP VAL ASN GLU CYS VAL SER ASN PRO CYS GLN          
SEQRES   5 A  116  ASN ASP ALA THR CYS LEU ASP GLN ILE GLY GLU PHE GLN          
SEQRES   6 A  116  CYS ILE CYS MET PRO GLY TYR GLU GLY VAL HIS CYS GLU          
SEQRES   7 A  116  VAL ASN THR ASP GLU CYS ALA SER SER PRO CYS LEU HIS          
SEQRES   8 A  116  ASN GLY ARG CYS LEU ASP LYS ILE ASN GLU PHE GLN CYS          
SEQRES   9 A  116  GLU CYS PRO THR GLY PHE THR GLY HIS LEU CYS GLN              
HELIX    1   1 ASP A  414  GLY A  419  1                                   6    
HELIX    2   2 GLU A  493  SER A  497  5                                   5    
SHEET    1   A 2 GLY A 427  ASN A 431  0                                        
SHEET    2   A 2 PHE A 436  CYS A 440 -1  O  GLU A 437   N  ILE A 430           
SHEET    1   B 2 THR A 466  GLN A 470  0                                        
SHEET    2   B 2 GLU A 473  ILE A 477 -1  O  GLU A 473   N  GLN A 470           
SHEET    1   C 2 ARG A 504  ASP A 507  0                                        
SHEET    2   C 2 PHE A 512  GLU A 515 -1  O  GLU A 515   N  ARG A 504           
SSBOND   1 CYS A  416    CYS A  429                          1555   1555  1.92  
SSBOND   2 CYS A  423    CYS A  438                          1555   1555  1.92  
SSBOND   3 CYS A  440    CYS A  449                          1555   1555  1.94  
SSBOND   4 CYS A  456    CYS A  467                          1555   1555  1.94  
SSBOND   5 CYS A  461    CYS A  476                          1555   1555  2.10  
SSBOND   6 CYS A  478    CYS A  487                          1555   1555  2.04  
SSBOND   7 CYS A  494    CYS A  505                          1555   1555  2.04  
SSBOND   8 CYS A  499    CYS A  514                          1555   1555  2.09  
SSBOND   9 CYS A  516    CYS A  525                          1555   1555  2.02  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system