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Database: PDB
Entry: 1TPG
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Original site: 1TPG 
HEADER    PLASMINOGEN ACTIVATION                  14-JUN-95   1TPG              
TITLE     F1-G MODULE PAIR RESIDUES 1-91 (C83S) OF TISSUE-TYPE                  
TITLE    2 PLASMINOGEN ACTIVATOR (T-PA) (NMR, 298K, PH2.95,                     
TITLE    3 REPRESENTATIVE STRUCTURE)                                            
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: T-PLASMINOGEN ACTIVATOR F1-G;                              
COMPND   3 CHAIN: A;                                                            
COMPND   4 EC: 3.4.21.68;                                                       
COMPND   5 ENGINEERED: YES;                                                     
COMPND   6 MUTATION: YES                                                        
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 EXPRESSION_SYSTEM: SACCHAROMYCES CEREVISIAE;                         
SOURCE   6 EXPRESSION_SYSTEM_COMMON: BAKER'S YEAST;                             
SOURCE   7 EXPRESSION_SYSTEM_TAXID: 4932;                                       
SOURCE   8 EXPRESSION_SYSTEM_PLASMID: PSW6                                      
KEYWDS    PLASMINOGEN ACTIVATION                                                
EXPDTA    SOLUTION NMR                                                          
AUTHOR    B.O.SMITH,A.K.DOWNING,P.C.DRISCOLL,T.J.DUDGEON,I.D.CAMPBELL           
REVDAT   2   24-FEB-09 1TPG    1       VERSN                                    
REVDAT   1   15-SEP-95 1TPG    0                                                
JRNL        AUTH   B.O.SMITH,A.K.DOWNING,P.C.DRISCOLL,T.J.DUDGEON,              
JRNL        AUTH 2 I.D.CAMPBELL                                                 
JRNL        TITL   THE SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF              
JRNL        TITL 2 THE FIBRONECTIN TYPE I AND EPIDERMAL GROWTH                  
JRNL        TITL 3 FACTOR-LIKE PAIR OF MODULES OF TISSUE-TYPE                   
JRNL        TITL 4 PLASMINOGEN ACTIVATOR.                                       
JRNL        REF    STRUCTURE                     V.   3   823 1995              
JRNL        REFN                   ISSN 0969-2126                               
JRNL        PMID   7582899                                                      
JRNL        DOI    10.1016/S0969-2126(01)00217-9                                
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   B.O.SMITH,A.K.DOWNING,T.J.DUDGEON,M.CUNNINGHAM,              
REMARK   1  AUTH 2 P.C.DRISCOLL,I.D.CAMPBELL                                    
REMARK   1  TITL   SECONDARY STRUCTURE OF FIBRONECTIN TYPE 1 AND                
REMARK   1  TITL 2 EPIDERMAL GROWTH FACTOR MODULES FROM TISSUE-TYPE             
REMARK   1  TITL 3 PLASMINOGEN ACTIVATOR BY NUCLEAR MAGNETIC RESONANCE          
REMARK   1  REF    BIOCHEMISTRY                  V.  33  2422 1994              
REMARK   1  REFN                   ISSN 0006-2960                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.0                                           
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1TPG COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY BNL.                                
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : NULL                               
REMARK 210  PH                             : NULL                               
REMARK 210  IONIC STRENGTH                 : NULL                               
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : NULL                               
REMARK 210  SPECTROMETER MODEL             : NULL                               
REMARK 210  SPECTROMETER MANUFACTURER      : NULL                               
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : NULL                               
REMARK 210   METHOD USED                   : NULL                               
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : NULL                               
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 1                                  
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL                
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    VAL A   4       88.75   -150.79                                   
REMARK 500    CYS A   6       97.82     83.02                                   
REMARK 500    LEU A  26      -91.33   -100.52                                   
REMARK 500    SER A  28      -80.50    -40.84                                   
REMARK 500    ASN A  29      -18.78    148.77                                   
REMARK 500    ALA A  41       95.61     58.81                                   
REMARK 500    HIS A  44      140.03   -178.37                                   
REMARK 500    PRO A  54       95.42    -65.73                                   
REMARK 500    PHE A  68     -169.60   -101.29                                   
REMARK 500    CYS A  75       85.53   -170.89                                   
REMARK 500    PRO A  76     -161.30    -64.46                                   
REMARK 500    GLU A  77      -54.63     87.28                                   
REMARK 500    PHE A  79     -148.70    -65.18                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: PLANAR GROUPS                                              
REMARK 500                                                                      
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL                 
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE                    
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN                    
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS                        
REMARK 500 AN RMSD GREATER THAN THIS VALUE                                      
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        RMS     TYPE                                    
REMARK 500    ARG A   7         0.30    SIDE_CHAIN                              
REMARK 500    ARG A  23         0.31    SIDE_CHAIN                              
REMARK 500    ARG A  27         0.25    SIDE_CHAIN                              
REMARK 500    ARG A  30         0.18    SIDE_CHAIN                              
REMARK 500    ARG A  40         0.28    SIDE_CHAIN                              
REMARK 500    ARG A  55         0.24    SIDE_CHAIN                              
REMARK 500    ARG A  89         0.25    SIDE_CHAIN                              
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
DBREF  1TPG A    1    91  UNP    P00750   TPA_HUMAN       36    126             
SEQADV 1TPG SER A   83  UNP  P00750    CYS   118 CONFLICT                       
SEQRES   1 A   91  SER TYR GLN VAL ILE CYS ARG ASP GLU LYS THR GLN MET          
SEQRES   2 A   91  ILE TYR GLN GLN HIS GLN SER TRP LEU ARG PRO VAL LEU          
SEQRES   3 A   91  ARG SER ASN ARG VAL GLU TYR CYS TRP CYS ASN SER GLY          
SEQRES   4 A   91  ARG ALA GLN CYS HIS SER VAL PRO VAL LYS SER CYS SER          
SEQRES   5 A   91  GLU PRO ARG CYS PHE ASN GLY GLY THR CYS GLN GLN ALA          
SEQRES   6 A   91  LEU TYR PHE SER ASP PHE VAL CYS GLN CYS PRO GLU GLY          
SEQRES   7 A   91  PHE ALA GLY LYS SER CYS GLU ILE ASP THR ARG ALA THR          
SHEET    1   A 2 SER A  20  PRO A  24  0                                        
SHEET    2   A 2 VAL A  31  TRP A  35 -1  N  CYS A  34   O  TRP A  21           
SHEET    1   B 3 VAL A  48  SER A  50  0                                        
SHEET    2   B 3 THR A  61  ALA A  65 -1  N  GLN A  64   O  LYS A  49           
SHEET    3   B 3 PHE A  71  GLN A  74 -1  N  GLN A  74   O  THR A  61           
SSBOND   1 CYS A    6    CYS A   36                          1555   1555  2.01  
SSBOND   2 CYS A   34    CYS A   43                          1555   1555  2.02  
SSBOND   3 CYS A   51    CYS A   62                          1555   1555  2.03  
SSBOND   4 CYS A   56    CYS A   73                          1555   1555  2.02  
SSBOND   5 CYS A   75    CYS A   84                          1555   1555  2.02  
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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