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Database: PDB
Entry: 1WGX
LinkDB: 1WGX
Original site: 1WGX 
HEADER    STRUCTURAL GENOMICS, UNKNOWN FUNCTION   28-MAY-04   1WGX              
TITLE     SOLUTION STRUCTURE OF RSGI RUH-022, A MYB DNA-BINDING                 
TITLE    2 DOMAIN IN HUMAN CDNA                                                 
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: KIAA1903 PROTEIN;                                          
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: MYB DNA BINDING DOMAIN;                                    
COMPND   5 SYNONYM: RSGI RUH-022 PROTEIN;                                       
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: KAZUSA CDNA 20103310H23;                                       
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040119-49;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    MYB DNA-BINDING DOMAIN, HUMAN CDNA, STRUCTURAL GENOMICS,              
KEYWDS   2 RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI,               
KEYWDS   3 UNKNOWN FUNCTION                                                     
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    Y.DOI-KATAYAMA,H.HIROTA,F.HAYASHI,S.YOKOYAMA,RIKEN                    
AUTHOR   2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)                     
REVDAT   2   24-FEB-09 1WGX    1       VERSN                                    
REVDAT   1   28-NOV-04 1WGX    0                                                
JRNL        AUTH   Y.DOI-KATAYAMA,H.HIROTA,F.HAYASHI,S.YOKOYAMA                 
JRNL        TITL   SOLUTION STRUCTURE OF RSGI RUH-022, A MYB                    
JRNL        TITL 2 DNA-BINDING DOMAIN IN HUMAN CDNA                             
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 1.0.7                                          
REMARK   3   AUTHORS     : GUENTERT, P.                                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1WGX COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 04-JUN-04.                  
REMARK 100 THE RCSB ID CODE IS RCSB023567.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 298                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM NACL                         
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.16MM MYB DNA-BINDING DOMAIN      
REMARK 210                                   U-15N,13C; 20MM D-TRIS-HCL (PH     
REMARK 210                                   7.0); 100MM NACL; 1MM D-DTT/       
REMARK 210                                   0.02% NAN3; 90% H2O, 10% D2O       
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1C, NMRPIPE 20020425,       
REMARK 210                                   NMRVIEW 5.0.4, KUJIRA 0.8972,      
REMARK 210                                   CYANA 1.0.7                        
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS,              
REMARK 210                                   STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY, TARGET FUNCTION            
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASP A   8      101.72   -176.63                                   
REMARK 500  1 LYS A   9       97.26    -45.01                                   
REMARK 500  1 CYS A  21      -70.57    -44.08                                   
REMARK 500  1 PRO A  31      -74.39    -75.02                                   
REMARK 500  1 SER A  43      -29.08    -38.76                                   
REMARK 500  1 ARG A  58      -45.60   -160.68                                   
REMARK 500  1 HIS A  65      -63.59   -101.85                                   
REMARK 500  1 THR A  67       72.63     42.75                                   
REMARK 500  1 SER A  72      -57.60   -135.54                                   
REMARK 500  2 SER A   2      113.05   -171.32                                   
REMARK 500  2 ASP A   8      144.86    179.65                                   
REMARK 500  2 CYS A  21      -70.52    -45.32                                   
REMARK 500  2 PRO A  31      -74.96    -75.01                                   
REMARK 500  2 SER A  43      -28.92    -38.57                                   
REMARK 500  2 ARG A  58       86.08   -151.55                                   
REMARK 500  2 LYS A  60      133.26   -178.30                                   
REMARK 500  2 GLN A  63      135.92     66.57                                   
REMARK 500  2 LYS A  64      104.42     60.51                                   
REMARK 500  2 HIS A  65      118.01   -166.57                                   
REMARK 500  2 VAL A  66      101.67     58.05                                   
REMARK 500  2 SER A  68       84.48     45.79                                   
REMARK 500  3 SER A   2      141.40    179.41                                   
REMARK 500  3 SER A   5      -57.77   -178.71                                   
REMARK 500  3 ASP A   8       87.84   -168.50                                   
REMARK 500  3 LYS A   9       57.05   -165.32                                   
REMARK 500  3 CYS A  21      -70.80    -47.67                                   
REMARK 500  3 PRO A  31      -71.72    -75.03                                   
REMARK 500  3 SER A  43      -28.76    -38.92                                   
REMARK 500  3 ARG A  58       86.53     45.63                                   
REMARK 500  3 LYS A  60      113.33   -163.19                                   
REMARK 500  3 SER A  62      144.56     61.54                                   
REMARK 500  3 SER A  68      164.52     62.54                                   
REMARK 500  3 SER A  71      153.88     66.32                                   
REMARK 500  3 SER A  72      124.09     64.29                                   
REMARK 500  4 SER A   2      107.62     59.56                                   
REMARK 500  4 SER A   3      167.21     60.70                                   
REMARK 500  4 SER A   6       97.59     50.41                                   
REMARK 500  4 CYS A  21      -70.96    -46.21                                   
REMARK 500  4 PRO A  31      -75.15    -75.01                                   
REMARK 500  4 SER A  43      -29.05    -38.73                                   
REMARK 500  4 ARG A  58      -51.78   -142.27                                   
REMARK 500  4 LYS A  60      128.77   -175.05                                   
REMARK 500  4 GLN A  63      153.57     68.62                                   
REMARK 500  4 SER A  71       75.59     74.23                                   
REMARK 500  5 SER A   5       90.32   -173.44                                   
REMARK 500  5 SER A   6      104.43   -168.29                                   
REMARK 500  5 ASP A   8      152.08    176.66                                   
REMARK 500  5 CYS A  21      -70.05    -42.41                                   
REMARK 500  5 PRO A  31      -76.77    -74.98                                   
REMARK 500  5 SER A  43      -28.39    -39.18                                   
REMARK 500  5 ARG A  58      -61.69   -130.20                                   
REMARK 500  5 LYS A  60      158.64     65.81                                   
REMARK 500  5 GLN A  63       97.56     64.11                                   
REMARK 500  5 HIS A  65      149.36   -170.58                                   
REMARK 500  5 THR A  67      116.44   -165.74                                   
REMARK 500  6 LYS A   9       55.86   -161.85                                   
REMARK 500  6 CYS A  21      -70.60    -43.41                                   
REMARK 500  6 SER A  43      -28.96    -38.50                                   
REMARK 500  6 PRO A  57     -162.97    -75.00                                   
REMARK 500  6 LYS A  60      -62.32   -141.13                                   
REMARK 500  6 GLN A  63      -58.98   -130.39                                   
REMARK 500  6 SER A  68      167.03     55.47                                   
REMARK 500  7 ASP A   8       94.34     55.76                                   
REMARK 500  7 LEU A  16      -70.43    -48.50                                   
REMARK 500  7 CYS A  21      -70.62    -41.97                                   
REMARK 500  7 PRO A  31      -83.06    -75.03                                   
REMARK 500  7 SER A  43      -28.88    -38.69                                   
REMARK 500  7 ARG A  58      100.87   -162.40                                   
REMARK 500  7 LYS A  60      167.60     62.05                                   
REMARK 500  7 HIS A  65      -61.50   -171.90                                   
REMARK 500  7 SER A  71      106.98     63.16                                   
REMARK 500  8 SER A   2       78.65   -173.23                                   
REMARK 500  8 SER A   3      112.69     62.65                                   
REMARK 500  8 LYS A   9     -155.69    -60.81                                   
REMARK 500  8 GLU A  10      171.81    -55.17                                   
REMARK 500  8 CYS A  21      -70.13    -42.65                                   
REMARK 500  8 PRO A  31      -71.94    -74.97                                   
REMARK 500  8 SER A  43      -29.03    -38.72                                   
REMARK 500  8 SER A  62       69.88     61.56                                   
REMARK 500  8 LYS A  64      152.44     70.39                                   
REMARK 500  8 SER A  72      -58.55   -137.98                                   
REMARK 500  9 SER A   2      153.64     60.60                                   
REMARK 500  9 SER A   3      111.15     62.71                                   
REMARK 500  9 SER A   6       77.15     41.21                                   
REMARK 500  9 LYS A   9       29.67   -159.00                                   
REMARK 500  9 CYS A  21      -70.66    -45.84                                   
REMARK 500  9 PRO A  31      -75.44    -74.97                                   
REMARK 500  9 SER A  43      -28.84    -38.88                                   
REMARK 500  9 PRO A  57     -162.90    -75.03                                   
REMARK 500  9 LYS A  60      170.88    -48.12                                   
REMARK 500  9 GLN A  63      141.74     66.85                                   
REMARK 500  9 VAL A  66      128.04     63.56                                   
REMARK 500 10 ASP A   8      102.29     58.96                                   
REMARK 500 10 GLU A  10     -176.12     48.12                                   
REMARK 500 10 CYS A  21      -70.72    -42.87                                   
REMARK 500 10 PRO A  31      -76.47    -74.96                                   
REMARK 500 10 SER A  43      -28.72    -38.77                                   
REMARK 500 10 ARG A  58      -56.91   -131.97                                   
REMARK 500 10 SER A  62      105.76   -176.69                                   
REMARK 500 10 GLN A  63      -61.69   -162.47                                   
REMARK 500 10 SER A  71      145.40    174.50                                   
REMARK 500 11 SER A   5      119.70     61.18                                   
REMARK 500 11 CYS A  21      -70.77    -47.32                                   
REMARK 500 11 PRO A  31      -79.93    -75.02                                   
REMARK 500 11 SER A  43      -28.13    -39.36                                   
REMARK 500 12 SER A   5       98.86   -166.11                                   
REMARK 500 12 SER A   6       96.16     54.59                                   
REMARK 500 12 LYS A   9     -159.91    -71.75                                   
REMARK 500 12 GLU A  10      174.51     58.08                                   
REMARK 500 12 CYS A  21      -70.81    -51.90                                   
REMARK 500 12 PRO A  31      -73.53    -75.05                                   
REMARK 500 12 SER A  43      -28.44    -39.22                                   
REMARK 500 12 PRO A  57     -164.10    -75.04                                   
REMARK 500 12 LYS A  60       89.56     45.97                                   
REMARK 500 12 SER A  62      -66.88   -154.60                                   
REMARK 500 12 HIS A  65       93.98     57.37                                   
REMARK 500 12 SER A  68      -54.07   -131.74                                   
REMARK 500 13 SER A   3      -58.93     74.57                                   
REMARK 500 13 SER A   5      102.60     58.70                                   
REMARK 500 13 SER A   6      164.23     58.32                                   
REMARK 500 13 ASP A   8      142.85    179.21                                   
REMARK 500 13 GLU A  10      158.47    -48.43                                   
REMARK 500 13 LEU A  16      -70.80    -53.44                                   
REMARK 500 13 CYS A  21      -70.89    -41.28                                   
REMARK 500 13 PRO A  31      -76.38    -75.01                                   
REMARK 500 13 SER A  43      -29.51    -37.94                                   
REMARK 500 13 ARG A  58       94.13    179.22                                   
REMARK 500 13 LYS A  60      134.68     68.59                                   
REMARK 500 13 LYS A  64       67.75   -172.70                                   
REMARK 500 13 HIS A  65       97.15   -162.77                                   
REMARK 500 14 CYS A  21      -71.02    -44.00                                   
REMARK 500 14 PRO A  31      -70.11    -75.02                                   
REMARK 500 14 SER A  43      -28.83    -38.71                                   
REMARK 500 14 ARG A  58      -60.65   -138.69                                   
REMARK 500 14 SER A  62      -58.58   -130.24                                   
REMARK 500 14 GLN A  63      134.56     65.14                                   
REMARK 500 14 LYS A  64      146.27     67.87                                   
REMARK 500 14 HIS A  65       87.67   -157.81                                   
REMARK 500 14 SER A  68      -65.08   -140.60                                   
REMARK 500 14 SER A  71      164.28     60.31                                   
REMARK 500 15 SER A   2      -58.73   -131.49                                   
REMARK 500 15 SER A   5       87.65     61.47                                   
REMARK 500 15 CYS A  21      -70.98    -44.56                                   
REMARK 500 15 PRO A  31      -70.19    -75.06                                   
REMARK 500 15 SER A  43      -28.56    -39.15                                   
REMARK 500 15 LYS A  60      -63.71   -135.22                                   
REMARK 500 15 VAL A  66       82.30     60.95                                   
REMARK 500 15 THR A  67      -61.41   -150.11                                   
REMARK 500 16 SER A   2       79.04     46.84                                   
REMARK 500 16 ASP A   8     -172.61     56.54                                   
REMARK 500 16 LEU A  16      -70.55    -41.74                                   
REMARK 500 16 CYS A  21      -70.90    -46.31                                   
REMARK 500 16 PRO A  31      -72.31    -75.03                                   
REMARK 500 16 SER A  43      -28.77    -38.71                                   
REMARK 500 16 PRO A  57     -164.69    -75.02                                   
REMARK 500 16 SER A  62      124.18   -179.15                                   
REMARK 500 16 LYS A  64       99.13   -171.97                                   
REMARK 500 16 SER A  68      140.06     65.57                                   
REMARK 500 16 SER A  72      -58.90   -125.08                                   
REMARK 500 17 SER A   3       81.45     53.54                                   
REMARK 500 17 GLU A  10      158.22    -39.19                                   
REMARK 500 17 CYS A  21      -70.62    -43.56                                   
REMARK 500 17 PRO A  31      -74.03    -75.02                                   
REMARK 500 17 SER A  43      -28.47    -39.27                                   
REMARK 500 17 SER A  62       79.29   -109.49                                   
REMARK 500 17 LYS A  64      144.03     64.76                                   
REMARK 500 17 VAL A  66       93.45     62.42                                   
REMARK 500 17 SER A  71      -83.50   -152.12                                   
REMARK 500 18 SER A   5       84.31     65.96                                   
REMARK 500 18 ASP A   8       95.60     57.61                                   
REMARK 500 18 GLU A  10      172.75    -52.50                                   
REMARK 500 18 GLU A  13      -30.14    -38.27                                   
REMARK 500 18 CYS A  21      -70.95    -47.20                                   
REMARK 500 18 PRO A  31      -77.60    -74.98                                   
REMARK 500 18 SER A  43      -28.13    -39.57                                   
REMARK 500 18 ARG A  58      -58.40   -164.97                                   
REMARK 500 18 LYS A  60      -63.14   -124.91                                   
REMARK 500 18 LYS A  64      103.14   -171.64                                   
REMARK 500 18 VAL A  66       90.74     51.92                                   
REMARK 500 18 SER A  68      -52.66   -153.66                                   
REMARK 500 18 SER A  71      -67.88   -170.04                                   
REMARK 500 19 SER A   2      112.02     64.15                                   
REMARK 500 19 SER A   6      -73.36     70.65                                   
REMARK 500 19 ASP A   8       87.34    -61.84                                   
REMARK 500 19 LYS A   9       75.73   -119.17                                   
REMARK 500 19 CYS A  21      -70.36    -45.89                                   
REMARK 500 19 PRO A  31      -75.96    -75.01                                   
REMARK 500 19 SER A  43      -28.61    -38.90                                   
REMARK 500 19 ARG A  58       94.24   -171.73                                   
REMARK 500 19 LYS A  60      -64.99    -99.18                                   
REMARK 500 19 SER A  62      131.65     63.51                                   
REMARK 500 19 SER A  71      -58.75   -173.02                                   
REMARK 500 20 SER A   2       89.81     47.65                                   
REMARK 500 20 GLU A  10      149.67   -178.09                                   
REMARK 500 20 LYS A  14      -71.93    -53.93                                   
REMARK 500 20 CYS A  21      -70.75    -46.14                                   
REMARK 500 20 PRO A  31      -79.10    -75.01                                   
REMARK 500 20 SER A  43      -28.54    -39.16                                   
REMARK 500 20 LYS A  60      -67.78     76.97                                   
REMARK 500 20 GLN A  63       79.16     65.22                                   
REMARK 500 20 LYS A  64       79.47   -159.73                                   
REMARK 500 20 THR A  67      137.87   -175.95                                   
REMARK 500 20 SER A  68      100.75   -176.03                                   
REMARK 500 20 SER A  71      -68.93     70.06                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 650                                                                      
REMARK 650 HELIX                                                                
REMARK 650 DETERMINATION METHOD: AUTHOR DETERMINED                              
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSK002001870.1   RELATED DB: TARGETDB                    
DBREF  1WGX A    8    67  UNP    Q6P0N0   CV106_HUMAN    759    818             
SEQADV 1WGX GLY A    1  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A    2  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A    3  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX GLY A    4  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A    5  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A    6  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX GLY A    7  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A   68  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX GLY A   69  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX PRO A   70  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A   71  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX SER A   72  UNP  Q6P0N0              CLONING ARTIFACT               
SEQADV 1WGX GLY A   73  UNP  Q6P0N0              CLONING ARTIFACT               
SEQRES   1 A   73  GLY SER SER GLY SER SER GLY ASP LYS GLU TRP ASN GLU          
SEQRES   2 A   73  LYS GLU LEU GLN LYS LEU HIS CYS ALA PHE ALA SER LEU          
SEQRES   3 A   73  PRO LYS HIS LYS PRO GLY PHE TRP SER GLU VAL ALA ALA          
SEQRES   4 A   73  ALA VAL GLY SER ARG SER PRO GLU GLU CYS GLN ARG LYS          
SEQRES   5 A   73  TYR MET GLU ASN PRO ARG GLY LYS GLY SER GLN LYS HIS          
SEQRES   6 A   73  VAL THR SER GLY PRO SER SER GLY                              
HELIX    1   1 GLU A   13  SER A   25  1                                  13    
HELIX    2   2 PHE A   33  ALA A   40  1                                   8    
HELIX    3   3 PRO A   46  GLU A   55  1                                  10    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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