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Entry: 1WI0
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HEADER    TRANSFERASE                             28-MAY-04   1WI0              
TITLE     SOLUTION STRUCTURE OF THE PB1 DOMAIN OF MOUSE MITOGEN                 
TITLE    2 ACTIVATED PROTEIN KINASE KINASE 5 (MAP2K5)                           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: MITOGEN ACTIVATED PROTEIN KINASE KINASE 5;                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: PB1 DOMAIN;                                                
COMPND   5 SYNONYM: MAP2K5;                                                     
COMPND   6 EC: 2.7.1.-;                                                         
COMPND   7 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: MUS MUSCULUS;                                   
SOURCE   3 ORGANISM_COMMON: HOUSE MOUSE;                                        
SOURCE   4 ORGANISM_TAXID: 10090;                                               
SOURCE   5 GENE: RIKEN CDNA A230106F01;                                         
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P031125-28;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    PB1 DOMAIN, PROTEIN-PROTEIN INTERACTION SITE, CYTOPLASMIC             
KEYWDS   2 SIGNALLING, STRUCTURAL GENOMICS, RIKEN STRUCTURAL                    
KEYWDS   3 GENOMICS/PROTEOMICS INITIATIVE, RSGI, TRANSFERASE                    
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    M.YONEYAMA,F.HAYASHI,N.TOCHIO,S.KOSHIBA,M.INOUE,T.KIGAWA,             
AUTHOR   2 S.YOKOYAMA,RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE           
AUTHOR   3 (RSGI)                                                               
REVDAT   3   24-FEB-09 1WI0    1       VERSN                                    
REVDAT   2   17-JAN-06 1WI0    1       TITLE  JRNL                              
REVDAT   1   28-NOV-04 1WI0    0                                                
JRNL        AUTH   M.YONEYAMA,F.HAYASHI,N.TOCHIO,S.KOSHIBA,M.INOUE,             
JRNL        AUTH 2 T.KIGAWA,S.YOKOYAMA                                          
JRNL        TITL   SOLUTION STRUCTURE OF THE PB1 DOMAIN OF MOUSE                
JRNL        TITL 2 MITOGEN ACTIVATED PROTEIN KINASE KINASE 5 (MAP2K5)           
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 1.0.8                                          
REMARK   3   AUTHORS     : GUENTERT, P.                                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1WI0 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 08-JUN-04.                  
REMARK 100 THE RCSB ID CODE IS RCSB023599.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 297                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1MM PB1 DOMAIN U-15N, 13C;         
REMARK 210                                   20MM D-TRIS-HCL; 100MM NACL;       
REMARK 210                                   1MM D-DTT; 0.02% NAN3; 10% D2O     
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_15N-SEPARATED_NOESY, 3D_        
REMARK 210                                   13C-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : INOVA                              
REMARK 210  SPECTROMETER MANUFACTURER      : VARIAN                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : VNMR 6.1C, NMRVIEW 5.0.4,          
REMARK 210                                   KUJIRA 0.896, CYANA 1.0.8,         
REMARK 210                                   NMRPIPE 20020425                   
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS, TARGET       
REMARK 210                                   FUNCTION                           
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 SER A   6       75.54     52.68                                   
REMARK 500  1 PHE A   9      -50.73   -136.07                                   
REMARK 500  1 CYS A  10      100.93     68.81                                   
REMARK 500  1 MET A  12      127.60   -175.12                                   
REMARK 500  1 GLN A  15      161.96    -44.65                                   
REMARK 500  1 SER A  26       36.40   -177.84                                   
REMARK 500  1 SER A  35       51.08    -91.14                                   
REMARK 500  1 THR A  56      173.40    -54.37                                   
REMARK 500  1 THR A  57      -74.24   -145.98                                   
REMARK 500  1 THR A  58      -55.71     75.39                                   
REMARK 500  1 ASP A  64     -152.37    -97.94                                   
REMARK 500  1 ARG A  69      100.60     55.19                                   
REMARK 500  1 GLN A  97      152.68    -40.62                                   
REMARK 500  1 LEU A 102       85.54    -58.41                                   
REMARK 500  1 ARG A 107       89.08    -55.34                                   
REMARK 500  1 SER A 112       81.08    178.74                                   
REMARK 500  2 SER A   2      -57.74   -151.32                                   
REMARK 500  2 SER A   3      154.28     62.55                                   
REMARK 500  2 SER A   6      -57.21   -129.84                                   
REMARK 500  2 CYS A  10      142.21   -179.92                                   
REMARK 500  2 ALA A  11      174.06     54.49                                   
REMARK 500  2 MET A  12      -54.12   -123.86                                   
REMARK 500  2 ASN A  14      -46.59   -133.68                                   
REMARK 500  2 ASN A  25       70.81     40.25                                   
REMARK 500  2 SER A  26      -42.46   -168.18                                   
REMARK 500  2 ALA A  28      144.19   -175.36                                   
REMARK 500  2 SER A  35       46.81    -98.62                                   
REMARK 500  2 GLN A  38       46.71    -85.41                                   
REMARK 500  2 THR A  56      173.44    -51.47                                   
REMARK 500  2 ASP A  64     -154.97    -86.31                                   
REMARK 500  2 ARG A  69       98.17     54.57                                   
REMARK 500  2 GLN A  97      152.07    -39.29                                   
REMARK 500  2 LEU A 102       84.81    -59.42                                   
REMARK 500  2 SER A 111      112.11   -178.66                                   
REMARK 500  2 SER A 112      112.19     60.57                                   
REMARK 500  3 SER A   5      -58.42   -125.65                                   
REMARK 500  3 GLU A  13       84.67   -178.10                                   
REMARK 500  3 ASN A  14       88.12     50.15                                   
REMARK 500  3 VAL A  16     -169.96     45.02                                   
REMARK 500  3 ASN A  25       74.99     39.31                                   
REMARK 500  3 SER A  26      -40.08   -174.46                                   
REMARK 500  3 SER A  35       45.61    -90.59                                   
REMARK 500  3 GLN A  38       43.33    -93.28                                   
REMARK 500  3 ARG A  69      105.10     56.27                                   
REMARK 500  3 GLN A  97      151.37    -39.70                                   
REMARK 500  3 LEU A 102       83.17    -60.86                                   
REMARK 500  3 SER A 111      128.73     63.05                                   
REMARK 500  4 ASN A  14      -50.77   -159.68                                   
REMARK 500  4 ASN A  25       71.99     41.63                                   
REMARK 500  4 SER A  26      -42.37   -170.57                                   
REMARK 500  4 ALA A  28      142.59   -178.50                                   
REMARK 500  4 THR A  32       99.74    -57.37                                   
REMARK 500  4 THR A  56      169.94    -45.27                                   
REMARK 500  4 ARG A  69      103.50     56.26                                   
REMARK 500  4 LEU A 102       81.63    -61.52                                   
REMARK 500  4 GLN A 103      106.51    -57.95                                   
REMARK 500  5 SER A   2      -58.60   -155.91                                   
REMARK 500  5 SER A   6      117.13   -166.91                                   
REMARK 500  5 ALA A  11      147.85   -176.06                                   
REMARK 500  5 MET A  12      -59.13   -166.82                                   
REMARK 500  5 GLU A  13      164.86     65.77                                   
REMARK 500  5 SER A  26       36.79   -178.49                                   
REMARK 500  5 SER A  35       46.45   -100.85                                   
REMARK 500  5 GLN A  38       38.37    -89.54                                   
REMARK 500  5 ALA A  55       79.33   -150.73                                   
REMARK 500  5 THR A  56      167.33    -46.17                                   
REMARK 500  5 ARG A  69      104.07     57.74                                   
REMARK 500  5 GLN A  97      159.49    -46.12                                   
REMARK 500  5 LEU A 102       83.52    -60.35                                   
REMARK 500  5 SER A 108      135.62     74.56                                   
REMARK 500  5 SER A 111      155.69     66.78                                   
REMARK 500  5 SER A 112      125.32   -176.49                                   
REMARK 500  6 MET A  12      131.32   -177.07                                   
REMARK 500  6 ASN A  14      169.07     60.75                                   
REMARK 500  6 GLN A  15      170.29    -45.43                                   
REMARK 500  6 ASN A  25       70.17     40.72                                   
REMARK 500  6 SER A  26      -48.43   -170.83                                   
REMARK 500  6 ALA A  28      140.37   -177.68                                   
REMARK 500  6 THR A  32       99.76    -59.37                                   
REMARK 500  6 THR A  56     -168.99    -56.28                                   
REMARK 500  6 THR A  57      -65.64   -160.91                                   
REMARK 500  6 THR A  58      -59.20     69.96                                   
REMARK 500  6 ASP A  64     -154.35   -104.45                                   
REMARK 500  6 ARG A  69       99.39     49.63                                   
REMARK 500  6 GLN A  97      159.24    -39.55                                   
REMARK 500  6 LEU A 102       84.59    -61.73                                   
REMARK 500  6 GLN A 103       91.48    -63.95                                   
REMARK 500  6 SER A 111      143.72   -177.50                                   
REMARK 500  6 SER A 112      110.31     63.08                                   
REMARK 500  7 SER A   3      149.49     63.17                                   
REMARK 500  7 GLU A  13       86.42    179.81                                   
REMARK 500  7 ASN A  14      159.84    174.60                                   
REMARK 500  7 GLN A  15      162.63    -44.22                                   
REMARK 500  7 SER A  26       49.44   -179.01                                   
REMARK 500  7 THR A  56      171.59    -59.63                                   
REMARK 500  7 THR A  57      -66.85   -143.03                                   
REMARK 500  7 THR A  58      -59.64     67.58                                   
REMARK 500  7 ARG A  69      105.34     57.70                                   
REMARK 500  7 LEU A 102       86.81    -56.80                                   
REMARK 500  7 ARG A 107      101.12    -56.26                                   
REMARK 500  7 SER A 111      -60.42   -126.64                                   
REMARK 500  8 SER A   3       97.52    -69.22                                   
REMARK 500  8 CYS A  10       96.71    -62.10                                   
REMARK 500  8 MET A  12       86.48     46.83                                   
REMARK 500  8 ASN A  14      -46.69   -153.07                                   
REMARK 500  8 LYS A  22       95.94    -69.47                                   
REMARK 500  8 SER A  26       51.20   -173.55                                   
REMARK 500  8 THR A  32      100.08    -59.53                                   
REMARK 500  8 SER A  35       48.40   -106.93                                   
REMARK 500  8 GLN A  38       46.92    -85.27                                   
REMARK 500  8 THR A  56      166.75    -47.09                                   
REMARK 500  8 ASP A  64     -162.38   -107.87                                   
REMARK 500  8 ARG A  69       98.88     53.17                                   
REMARK 500  8 GLN A  97      151.42    -40.47                                   
REMARK 500  8 LEU A 102       93.29    -49.58                                   
REMARK 500  8 SER A 108      -78.19    -96.14                                   
REMARK 500  8 SER A 111      150.02    -45.34                                   
REMARK 500  8 SER A 112      -53.97   -170.16                                   
REMARK 500  9 SER A   2       90.86    -63.31                                   
REMARK 500  9 SER A   5       85.80   -175.96                                   
REMARK 500  9 SER A   6      119.42   -169.74                                   
REMARK 500  9 CYS A  10      -48.00   -159.24                                   
REMARK 500  9 MET A  12      -47.66   -142.77                                   
REMARK 500  9 ASN A  25       72.29     43.96                                   
REMARK 500  9 SER A  26      -45.02   -170.91                                   
REMARK 500  9 ALA A  28      148.01   -176.08                                   
REMARK 500  9 SER A  35       51.70    -93.40                                   
REMARK 500  9 THR A  56      165.75    -45.51                                   
REMARK 500  9 ASP A  64     -151.29    -78.68                                   
REMARK 500  9 ARG A  69       89.74    -59.39                                   
REMARK 500  9 LEU A 102       87.81    -56.40                                   
REMARK 500  9 SER A 108      150.87     63.89                                   
REMARK 500  9 SER A 111       72.13     39.02                                   
REMARK 500 10 SER A   2      -57.98   -141.30                                   
REMARK 500 10 SER A   5      -57.60   -142.42                                   
REMARK 500 10 PHE A   9      145.88   -170.21                                   
REMARK 500 10 CYS A  10      -67.07     73.12                                   
REMARK 500 10 ALA A  11      119.82     64.95                                   
REMARK 500 10 ASN A  14      -58.73   -173.79                                   
REMARK 500 10 SER A  26       43.58   -176.36                                   
REMARK 500 10 GLN A  38       40.24    -95.40                                   
REMARK 500 10 THR A  56      167.21    -49.02                                   
REMARK 500 10 ASP A  64     -152.70    -74.70                                   
REMARK 500 10 ARG A  69       99.83    -57.61                                   
REMARK 500 10 GLN A  97      150.72    -38.57                                   
REMARK 500 10 LEU A 102       83.26    -60.79                                   
REMARK 500 10 SER A 111      -59.52     77.21                                   
REMARK 500 10 SER A 112      -59.14   -166.67                                   
REMARK 500 11 SER A   2      109.65     61.77                                   
REMARK 500 11 SER A   5      117.98   -168.07                                   
REMARK 500 11 CYS A  10       67.24   -159.73                                   
REMARK 500 11 MET A  12      150.62     60.47                                   
REMARK 500 11 GLN A  15      152.90    -39.44                                   
REMARK 500 11 LYS A  22       93.82    -66.13                                   
REMARK 500 11 ASN A  25       72.50     54.45                                   
REMARK 500 11 SER A  26      -60.05   -172.22                                   
REMARK 500 11 ALA A  28      130.57   -174.93                                   
REMARK 500 11 THR A  56      150.32    -44.06                                   
REMARK 500 11 ARG A  69      105.45     56.33                                   
REMARK 500 11 GLN A  97      160.34    -40.30                                   
REMARK 500 11 LEU A 102       88.17    -56.21                                   
REMARK 500 12 SER A   3      113.35   -160.32                                   
REMARK 500 12 ASN A  14      171.93    177.39                                   
REMARK 500 12 GLN A  15      146.66     63.08                                   
REMARK 500 12 VAL A  16     -179.27    -62.86                                   
REMARK 500 12 ASN A  25       25.22     48.64                                   
REMARK 500 12 VAL A  33      -66.08   -120.40                                   
REMARK 500 12 HIS A  34      -53.38     76.33                                   
REMARK 500 12 GLN A  38       45.80    -96.15                                   
REMARK 500 12 VAL A  47      -60.89    -90.28                                   
REMARK 500 12 THR A  56      162.59    -48.50                                   
REMARK 500 12 ASP A  64     -153.44   -104.70                                   
REMARK 500 12 ARG A  69      102.93     52.50                                   
REMARK 500 12 GLN A  97      152.70    -39.56                                   
REMARK 500 12 LEU A 102       89.27    -55.44                                   
REMARK 500 12 GLN A 103       99.95    -63.95                                   
REMARK 500 12 SER A 112      117.68     60.02                                   
REMARK 500 13 SER A   2      -58.23   -178.08                                   
REMARK 500 13 CYS A  10      -69.28   -105.68                                   
REMARK 500 13 ALA A  11      109.58     65.92                                   
REMARK 500 13 GLN A  15      148.99     67.32                                   
REMARK 500 13 VAL A  16     -166.14     43.99                                   
REMARK 500 13 ASN A  25       73.28     39.93                                   
REMARK 500 13 SER A  26      -56.93   -174.96                                   
REMARK 500 13 ALA A  28      148.66   -170.00                                   
REMARK 500 13 THR A  32      100.25    -59.33                                   
REMARK 500 13 SER A  35       53.24    -90.79                                   
REMARK 500 13 GLN A  38       42.30    -91.92                                   
REMARK 500 13 THR A  56     -173.46    -59.00                                   
REMARK 500 13 THR A  57      -84.10   -157.10                                   
REMARK 500 13 THR A  58      -55.92     83.59                                   
REMARK 500 13 ARG A  69      105.08     55.93                                   
REMARK 500 13 LEU A 102       80.91    -61.69                                   
REMARK 500 13 ARG A 107       87.14    -56.53                                   
REMARK 500 13 SER A 112      157.55     60.04                                   
REMARK 500 14 SER A   2      -58.43   -172.01                                   
REMARK 500 14 CYS A  10       98.73   -167.36                                   
REMARK 500 14 MET A  12      129.86   -178.52                                   
REMARK 500 14 GLU A  13       65.08   -166.30                                   
REMARK 500 14 ASN A  14       95.26     64.26                                   
REMARK 500 14 GLN A  15      164.96    177.13                                   
REMARK 500 14 VAL A  16     -169.76     45.09                                   
REMARK 500 14 ASN A  25       76.02     39.78                                   
REMARK 500 14 SER A  26      -52.99   -175.89                                   
REMARK 500 14 ALA A  28      146.39   -171.51                                   
REMARK 500 14 SER A  35       48.17    -95.15                                   
REMARK 500 14 GLN A  38       38.44    -89.40                                   
REMARK 500 14 THR A  56      174.50    -56.04                                   
REMARK 500 14 THR A  57      -81.36   -150.41                                   
REMARK 500 14 THR A  58      -51.66     80.45                                   
REMARK 500 14 ASP A  64     -154.92   -104.32                                   
REMARK 500 14 ARG A  69       98.75     58.93                                   
REMARK 500 14 GLN A  97      154.31    -45.48                                   
REMARK 500 14 LEU A 102       78.59    -63.86                                   
REMARK 500 14 GLN A 103      104.33    -56.59                                   
REMARK 500 14 ARG A 107       92.74    -68.74                                   
REMARK 500 14 SER A 111      149.23   -178.42                                   
REMARK 500 14 SER A 112       82.36   -174.09                                   
REMARK 500 15 PHE A   9      176.76     51.41                                   
REMARK 500 15 GLU A  13      -52.60   -174.05                                   
REMARK 500 15 ASN A  14      -53.29   -179.94                                   
REMARK 500 15 ASN A  25       74.21     44.38                                   
REMARK 500 15 SER A  26      -43.66   -172.82                                   
REMARK 500 15 ALA A  28      144.63   -176.00                                   
REMARK 500 15 THR A  32       99.86    -63.24                                   
REMARK 500 15 SER A  35       53.09    -99.82                                   
REMARK 500 15 GLN A  97      151.79    -38.56                                   
REMARK 500 15 LEU A 102       84.77    -60.45                                   
REMARK 500 15 SER A 111      100.48     61.00                                   
REMARK 500 16 SER A   5      -59.34   -175.40                                   
REMARK 500 16 SER A   6      157.51     63.74                                   
REMARK 500 16 CYS A  10       77.76     62.42                                   
REMARK 500 16 ALA A  11      -59.93   -166.54                                   
REMARK 500 16 GLU A  13      -38.49   -176.93                                   
REMARK 500 16 GLN A  15      171.82    -49.24                                   
REMARK 500 16 LYS A  22       96.61    -63.18                                   
REMARK 500 16 SER A  26     -157.04   -155.84                                   
REMARK 500 16 GLN A  38       41.14    -88.35                                   
REMARK 500 16 VAL A  47      -60.60    -91.50                                   
REMARK 500 16 ALA A  55       79.02   -152.80                                   
REMARK 500 16 THR A  56      173.78    -47.34                                   
REMARK 500 16 ASP A  64     -151.43    -99.23                                   
REMARK 500 16 ARG A  69       98.44    -64.03                                   
REMARK 500 16 GLN A  97      151.27    -38.43                                   
REMARK 500 16 LEU A 102       92.58    -51.30                                   
REMARK 500 16 GLN A 103       89.79    -69.79                                   
REMARK 500 16 SER A 108      148.58     65.47                                   
REMARK 500 16 SER A 112      -73.18     73.70                                   
REMARK 500 17 SER A   2       79.53     48.57                                   
REMARK 500 17 GLU A  13      137.87   -170.15                                   
REMARK 500 17 ASN A  25       73.90     40.02                                   
REMARK 500 17 SER A  26      -71.30   -171.01                                   
REMARK 500 17 GLN A  38       50.99    -97.12                                   
REMARK 500 17 THR A  57      -82.30   -129.41                                   
REMARK 500 17 THR A  58       15.89     59.41                                   
REMARK 500 17 ALA A  59      148.80    168.35                                   
REMARK 500 17 ASP A  64     -153.10   -102.90                                   
REMARK 500 17 ARG A  69      101.52     56.42                                   
REMARK 500 17 GLN A  97      159.94    -43.09                                   
REMARK 500 17 LEU A 102       86.79    -59.39                                   
REMARK 500 17 SER A 111      145.27     67.01                                   
REMARK 500 18 SER A   5      -56.19   -144.75                                   
REMARK 500 18 CYS A  10      109.07   -173.31                                   
REMARK 500 18 MET A  12      -61.93   -143.36                                   
REMARK 500 18 VAL A  16     -169.48     46.80                                   
REMARK 500 18 ASN A  25       71.21     51.28                                   
REMARK 500 18 SER A  26      -99.63   -172.69                                   
REMARK 500 18 ALA A  28      126.82   -174.03                                   
REMARK 500 18 SER A  35       45.91    -87.58                                   
REMARK 500 18 GLN A  38       38.71    -94.79                                   
REMARK 500 18 THR A  56     -179.01    -51.50                                   
REMARK 500 18 THR A  57      131.94   -170.43                                   
REMARK 500 18 ASP A  64     -152.51    -94.32                                   
REMARK 500 18 ARG A  69       99.08     54.27                                   
REMARK 500 18 GLN A  97      156.17    -39.09                                   
REMARK 500 18 LEU A 102       87.09    -57.16                                   
REMARK 500 18 SER A 108      119.33     81.19                                   
REMARK 500 18 SER A 112      171.30     58.80                                   
REMARK 500 19 SER A   3      143.03     62.66                                   
REMARK 500 19 SER A   5      168.92     58.61                                   
REMARK 500 19 MET A  12       51.12   -177.92                                   
REMARK 500 19 GLU A  13       67.76   -101.05                                   
REMARK 500 19 ASN A  14      161.78    179.18                                   
REMARK 500 19 SER A  26      -62.94   -170.27                                   
REMARK 500 19 THR A  32       99.83    -64.22                                   
REMARK 500 19 SER A  35       52.93    -98.85                                   
REMARK 500 19 THR A  56      175.11    -48.01                                   
REMARK 500 19 ALA A  59      157.19    178.35                                   
REMARK 500 19 ARG A  69      101.43     59.17                                   
REMARK 500 19 GLN A  97      149.80    -38.58                                   
REMARK 500 19 LEU A 102       77.96    -64.48                                   
REMARK 500 20 SER A   2      -58.61   -127.72                                   
REMARK 500 20 SER A   3      153.71     64.98                                   
REMARK 500 20 SER A   5       89.20   -166.21                                   
REMARK 500 20 MET A  12      -71.17   -142.81                                   
REMARK 500 20 GLU A  13      176.32     69.47                                   
REMARK 500 20 ASN A  25       51.99     39.94                                   
REMARK 500 20 SER A  26      146.78   -178.72                                   
REMARK 500 20 SER A  35       49.42    -90.19                                   
REMARK 500 20 THR A  56     -179.55    -59.57                                   
REMARK 500 20 THR A  57      -79.01   -147.10                                   
REMARK 500 20 ALA A  59      154.17    164.06                                   
REMARK 500 20 ARG A  69       99.94     61.16                                   
REMARK 500 20 LEU A 102       85.39    -58.32                                   
REMARK 500 20 GLN A 103       98.29    -62.58                                   
REMARK 500 20 ARG A 107       99.85    -65.98                                   
REMARK 500 20 SER A 112      146.70     60.78                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: MMT007017439.1   RELATED DB: TARGETDB                    
DBREF  1WI0 A    8   107  UNP    Q9WVS7   MP2K5_MOUSE      8    107             
SEQADV 1WI0 GLY A    1  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A    2  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A    3  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 GLY A    4  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A    5  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A    6  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 GLY A    7  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A  108  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 GLY A  109  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 PRO A  110  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A  111  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 SER A  112  UNP  Q9WVS7              CLONING ARTIFACT               
SEQADV 1WI0 GLY A  113  UNP  Q9WVS7              CLONING ARTIFACT               
SEQRES   1 A  113  GLY SER SER GLY SER SER GLY PRO PHE CYS ALA MET GLU          
SEQRES   2 A  113  ASN GLN VAL LEU VAL ILE ARG ILE LYS ILE PRO ASN SER          
SEQRES   3 A  113  GLY ALA VAL ASP TRP THR VAL HIS SER GLY PRO GLN LEU          
SEQRES   4 A  113  LEU PHE ARG ASP VAL LEU ASP VAL ILE GLY GLN VAL LEU          
SEQRES   5 A  113  PRO GLU ALA THR THR THR ALA PHE GLU TYR GLU ASP GLU          
SEQRES   6 A  113  ASP GLY ASP ARG ILE THR VAL ARG SER ASP GLU GLU MET          
SEQRES   7 A  113  LYS ALA MET LEU SER TYR TYR TYR SER THR VAL MET GLU          
SEQRES   8 A  113  GLN GLN VAL ASN GLY GLN LEU ILE GLU PRO LEU GLN ILE          
SEQRES   9 A  113  PHE PRO ARG SER GLY PRO SER SER GLY                          
HELIX    1   1 LEU A   40  LEU A   52  1                                  13    
HELIX    2   2 SER A   74  GLY A   96  1                                  23    
SHEET    1   A 5 ALA A  28  VAL A  33  0                                        
SHEET    2   A 5 LEU A  17  LYS A  22 -1  N  LEU A  17   O  VAL A  33           
SHEET    3   A 5 LEU A 102  PRO A 106  1  O  ILE A 104   N  LYS A  22           
SHEET    4   A 5 PHE A  60  TYR A  62 -1  N  GLU A  61   O  PHE A 105           
SHEET    5   A 5 ILE A  70  VAL A  72 -1  O  VAL A  72   N  PHE A  60           
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
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