GenomeNet

Database: PDB
Entry: 1WJO
LinkDB: 1WJO
Original site: 1WJO 
HEADER    PROTEIN BINDING                         29-MAY-04   1WJO              
TITLE     SOLUTION STRUCTURE OF THE FORTH CH DOMAIN FROM HUMAN                  
TITLE    2 PLASTIN 3 T-ISOFORM                                                  
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: T-PLASTIN;                                                 
COMPND   3 CHAIN: A;                                                            
COMPND   4 FRAGMENT: CH DOMAIN;                                                 
COMPND   5 SYNONYM: PLASTIN 3 T-ISOFORM;                                        
COMPND   6 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 ORGANISM_TAXID: 9606;                                                
SOURCE   5 GENE: IMS CDNA HEP01557;                                             
SOURCE   6 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;                              
SOURCE   7 EXPRESSION_SYSTEM_PLASMID: P040223-07;                               
SOURCE   8 OTHER_DETAILS: CELL-FREE PROTEIN SYNTHESIS                           
KEYWDS    CH DOMAIN, ACTIN BINDING, PLASTIN 3, STRUCTURAL GENOMICS,             
KEYWDS   2 RIKEN STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE, RSGI,               
KEYWDS   3 PROTEIN BINDING                                                      
EXPDTA    SOLUTION NMR                                                          
NUMMDL    20                                                                    
AUTHOR    T.TOMIZAWA,T.KIGAWA,S.KOSHIBA,M.INOUE,S.YOKOYAMA,RIKEN                
AUTHOR   2 STRUCTURAL GENOMICS/PROTEOMICS INITIATIVE (RSGI)                     
REVDAT   2   24-FEB-09 1WJO    1       VERSN                                    
REVDAT   1   29-NOV-04 1WJO    0                                                
JRNL        AUTH   T.TOMIZAWA,T.KIGAWA,S.KOSHIBA,M.INOUE,S.YOKOYAMA             
JRNL        TITL   SOLUTION STRUCTURE OF THE FORTH CH DOMAIN FROM               
JRNL        TITL 2 HUMAN PLASTIN 3 T-ISOFORM                                    
JRNL        REF    TO BE PUBLISHED                                              
JRNL        REFN                                                                
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : CYANA 2.0.17                                         
REMARK   3   AUTHORS     : GUENTERT, P.                                         
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1WJO COMPLIES WITH FORMAT V. 3.15, 01-DEC-08                         
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 09-JUN-04.                  
REMARK 100 THE RCSB ID CODE IS RCSB023648.                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 296                                
REMARK 210  PH                             : 7.0                                
REMARK 210  IONIC STRENGTH                 : 120MM                              
REMARK 210  PRESSURE                       : AMBIENT                            
REMARK 210  SAMPLE CONTENTS                : 1.16MM CH DOMAIN U-15N,13C;        
REMARK 210                                   20MM D-TRIS-HCL(PH 7.0); 100MM     
REMARK 210                                   NACL; 1MM D-DTT; 0.02% NAN3;       
REMARK 210                                   10% D2O                            
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : 3D_13C-SEPARATED_NOESY, 3D_        
REMARK 210                                   15N-SEPARATED_NOESY                
REMARK 210  SPECTROMETER FIELD STRENGTH    : 800 MHZ                            
REMARK 210  SPECTROMETER MODEL             : AVANCE                             
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER                             
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XWINNMR 2.6, NMRPIPE               
REMARK 210                                   20030801, NMRVIEW 5.0.4,           
REMARK 210                                   KUJIRA 0.901, CYANA 2.0.17         
REMARK 210   METHOD USED                   : TORSION ANGLE DYNAMICS             
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 100                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 20                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : STRUCTURES WITH THE LEAST          
REMARK 210                                   RESTRAINT VIOLATIONS,              
REMARK 210                                   STRUCTURES WITH THE LOWEST         
REMARK 210                                   ENERGY, TARGET FUNCTION            
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: NULL                                                         
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-           
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400            
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500  1 ASP A  58      -36.02    -38.56                                   
REMARK 500  1 ARG A  87       42.48     37.51                                   
REMARK 500  2 ASP A  58      -37.85    -39.46                                   
REMARK 500  2 GLU A  67      -31.15    -37.73                                   
REMARK 500  2 ASP A  68      -73.19    -84.12                                   
REMARK 500  2 VAL A  78      -71.99    -55.93                                   
REMARK 500  2 TYR A  89       48.41     35.33                                   
REMARK 500  2 PRO A 100        1.81    -69.76                                   
REMARK 500  2 LYS A 116      135.55    -37.39                                   
REMARK 500  3 ASP A   9      -53.62   -127.35                                   
REMARK 500  3 SER A  26       30.48    -96.77                                   
REMARK 500  3 SER A  28      148.32   -170.79                                   
REMARK 500  3 ASP A  34      150.55    -46.19                                   
REMARK 500  3 ASP A  58      -34.29    -39.66                                   
REMARK 500  3 TYR A  89       39.06    -84.51                                   
REMARK 500  3 LEU A  91      150.84    -47.74                                   
REMARK 500  3 MET A 115      110.82    -34.86                                   
REMARK 500  3 SER A 122       95.34    -39.07                                   
REMARK 500  4 SER A   5      161.72    -45.95                                   
REMARK 500  4 ASN A   8       66.72   -108.14                                   
REMARK 500  4 ASP A   9      -61.63    -98.94                                   
REMARK 500  4 TYR A  89       37.57    -85.92                                   
REMARK 500  4 PRO A 121       92.26    -69.78                                   
REMARK 500  5 ASP A   9      -59.69   -125.53                                   
REMARK 500  5 GLU A  22      -39.96    -36.87                                   
REMARK 500  5 SER A  31     -176.39   -170.11                                   
REMARK 500  5 ASP A  34      156.71    -43.17                                   
REMARK 500  5 ASP A  58      -33.83    -38.76                                   
REMARK 500  5 GLU A  67      -37.11    -36.87                                   
REMARK 500  5 ALA A  86      101.06    -35.13                                   
REMARK 500  5 TYR A  89       46.01     35.01                                   
REMARK 500  5 LEU A  91      143.99    -38.02                                   
REMARK 500  5 SER A 122       48.95    -92.54                                   
REMARK 500  6 SER A   3      102.38    -54.58                                   
REMARK 500  6 ASN A   8      -71.00    -84.64                                   
REMARK 500  6 ASP A   9      -62.42   -128.37                                   
REMARK 500  6 SER A  28      146.55   -172.56                                   
REMARK 500  6 ASP A  58      -31.98    -36.91                                   
REMARK 500  6 GLU A  67      -36.71    -36.83                                   
REMARK 500  6 VAL A  88      107.08    -39.62                                   
REMARK 500  6 PRO A 100        2.56    -69.74                                   
REMARK 500  6 MET A 102      -33.70   -133.42                                   
REMARK 500  6 CYS A 109      -32.16    -36.53                                   
REMARK 500  6 MET A 115      103.06    -41.76                                   
REMARK 500  6 LYS A 116       80.63    -60.63                                   
REMARK 500  7 SER A   5      145.29    -34.72                                   
REMARK 500  7 GLU A  22      -35.74    -39.13                                   
REMARK 500  7 ASP A  58      -33.68    -38.79                                   
REMARK 500  7 ASN A  64       86.63    -69.84                                   
REMARK 500  7 GLU A  67      -38.14    -38.81                                   
REMARK 500  7 ARG A  87      -38.37    -37.39                                   
REMARK 500  7 VAL A  88      112.82    -36.95                                   
REMARK 500  7 PRO A 100        1.49    -69.79                                   
REMARK 500  7 SER A 119      106.04    -49.74                                   
REMARK 500  8 SER A  26       41.35    -92.35                                   
REMARK 500  8 SER A  62      141.68   -170.52                                   
REMARK 500  8 ARG A  87       48.64    -86.88                                   
REMARK 500  8 LEU A  91      150.43    -47.28                                   
REMARK 500  8 PRO A 100        1.47    -69.81                                   
REMARK 500  8 MET A 115      124.23    -34.44                                   
REMARK 500  8 SER A 119      116.03   -171.56                                   
REMARK 500  8 PRO A 121       98.51    -69.71                                   
REMARK 500  9 ASN A   8       46.60   -103.28                                   
REMARK 500  9 GLU A  22      -36.05    -35.91                                   
REMARK 500  9 ASP A  34      154.42    -40.77                                   
REMARK 500  9 ASP A  58      -32.70    -36.89                                   
REMARK 500  9 ASP A  68      -70.02    -73.24                                   
REMARK 500  9 LYS A  99      105.40    -47.67                                   
REMARK 500 10 SER A  28      145.98   -171.56                                   
REMARK 500 10 ASP A  34      151.22    -36.99                                   
REMARK 500 10 ASP A  58      -38.17    -34.86                                   
REMARK 500 10 ASN A  64       81.51    -69.54                                   
REMARK 500 10 GLU A  67      -36.15    -37.21                                   
REMARK 500 10 ALA A  86      102.47    -42.51                                   
REMARK 500 10 TYR A  89       51.52     35.79                                   
REMARK 500 10 LEU A  91      145.77    -39.26                                   
REMARK 500 10 PRO A 100        2.90    -69.83                                   
REMARK 500 10 SER A 123       41.58     37.04                                   
REMARK 500 11 SER A   5      101.53    -37.82                                   
REMARK 500 11 ASN A  64       85.42    -69.40                                   
REMARK 500 11 TYR A  89       44.34   -101.90                                   
REMARK 500 11 PRO A 100        0.99    -69.76                                   
REMARK 500 11 CYS A 109      -38.90    -39.56                                   
REMARK 500 12 ILE A  11      -38.71    -38.66                                   
REMARK 500 12 GLU A  22      -39.51    -38.30                                   
REMARK 500 12 SER A  26       34.76    -98.99                                   
REMARK 500 12 ASP A  34      164.25    -43.02                                   
REMARK 500 12 ASP A  58      -32.97    -38.17                                   
REMARK 500 12 ASN A  64       93.87    -34.57                                   
REMARK 500 12 ARG A  87       55.11    -99.72                                   
REMARK 500 12 TYR A  89       34.88     34.39                                   
REMARK 500 12 PRO A 100        1.93    -69.74                                   
REMARK 500 12 MET A 115      100.79    -59.50                                   
REMARK 500 12 ARG A 117      158.43    -43.08                                   
REMARK 500 12 PRO A 121     -177.92    -69.77                                   
REMARK 500 13 ASP A  34      150.47    -38.02                                   
REMARK 500 13 ASP A  58      -38.67    -37.44                                   
REMARK 500 13 TYR A  89       34.31    -84.55                                   
REMARK 500 13 PRO A 100        1.61    -69.79                                   
REMARK 500 13 MET A 115      100.07    -45.19                                   
REMARK 500 13 ARG A 117      114.76   -174.42                                   
REMARK 500 13 VAL A 118       46.95     34.51                                   
REMARK 500 13 SER A 122       88.65    -69.56                                   
REMARK 500 14 SER A   3      104.37    -57.67                                   
REMARK 500 14 SER A   6      -50.57   -126.34                                   
REMARK 500 14 ASP A  34      150.66    -44.09                                   
REMARK 500 14 ASP A  58      -33.58    -36.23                                   
REMARK 500 14 ALA A  86       98.67    -45.32                                   
REMARK 500 14 TYR A  89       46.81   -109.81                                   
REMARK 500 14 LEU A  91      151.97    -36.37                                   
REMARK 500 14 PRO A 100        1.43    -69.73                                   
REMARK 500 14 SER A 119      132.93   -174.99                                   
REMARK 500 15 SER A   6       96.51    -63.74                                   
REMARK 500 15 ASP A  58      -35.45    -35.98                                   
REMARK 500 15 TYR A  89       44.56    -80.66                                   
REMARK 500 15 MET A 115      116.48    -38.87                                   
REMARK 500 16 SER A  28      144.84   -174.23                                   
REMARK 500 16 ASP A  58      -30.21    -36.88                                   
REMARK 500 16 GLU A  67      -36.33    -38.18                                   
REMARK 500 16 VAL A  88      117.85    -37.01                                   
REMARK 500 16 PRO A 100        2.59    -69.78                                   
REMARK 500 16 ALA A 108      -39.12    -38.98                                   
REMARK 500 17 SER A   2       50.91    -96.92                                   
REMARK 500 17 LEU A  20      -39.71    -35.74                                   
REMARK 500 17 ASP A  58      -37.77    -36.24                                   
REMARK 500 17 SER A  62      137.81   -171.23                                   
REMARK 500 17 GLU A  67      -35.96    -34.61                                   
REMARK 500 17 TYR A  89       43.95    -80.19                                   
REMARK 500 17 PRO A 100        2.83    -69.76                                   
REMARK 500 17 MET A 102      -39.00   -131.76                                   
REMARK 500 17 ARG A 113      -66.28   -107.46                                   
REMARK 500 17 ARG A 117       39.97    -83.87                                   
REMARK 500 18 SER A  28      148.17   -172.21                                   
REMARK 500 18 ASP A  34      152.84    -46.77                                   
REMARK 500 18 ASP A  58      -32.91    -36.05                                   
REMARK 500 18 ALA A  81      -37.47    -35.29                                   
REMARK 500 18 ALA A  86       95.97    -36.63                                   
REMARK 500 18 ARG A  87       76.20    -68.73                                   
REMARK 500 18 TYR A  89       28.62     43.13                                   
REMARK 500 18 VAL A 103      -19.69    -47.51                                   
REMARK 500 19 ASN A   8     -175.97   -172.39                                   
REMARK 500 19 GLU A  22      -36.26    -34.50                                   
REMARK 500 19 ASP A  34      152.75    -36.05                                   
REMARK 500 19 ARG A  87       81.72    -68.25                                   
REMARK 500 19 TYR A  89       32.96    -84.37                                   
REMARK 500 19 LYS A  99      105.97    -48.83                                   
REMARK 500 19 PRO A 100        3.21    -69.74                                   
REMARK 500 20 ASP A  58      -34.20    -37.96                                   
REMARK 500 20 THR A  66     -178.70    -63.05                                   
REMARK 500 20 TYR A  89       48.56    -77.91                                   
REMARK 500 20 PRO A 100        1.28    -69.73                                   
REMARK 500 20 VAL A 118      166.65    -43.31                                   
REMARK 500 20 SER A 122      125.03    -34.90                                   
REMARK 500                                                                      
REMARK 500 REMARK: NULL                                                         
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: HSS001001434.1   RELATED DB: TARGETDB                    
DBREF  1WJO A    8   118  UNP    P13797   PLST_HUMAN     517    627             
SEQADV 1WJO GLY A    1  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A    2  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A    3  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO GLY A    4  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A    5  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A    6  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO GLY A    7  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A  119  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO GLY A  120  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO PRO A  121  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A  122  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO SER A  123  UNP  P13797              CLONING ARTIFACT               
SEQADV 1WJO GLY A  124  UNP  P13797              CLONING ARTIFACT               
SEQRES   1 A  124  GLY SER SER GLY SER SER GLY ASN ASP ASP ILE ILE VAL          
SEQRES   2 A  124  ASN TRP VAL ASN ARG THR LEU SER GLU ALA GLY LYS SER          
SEQRES   3 A  124  THR SER ILE GLN SER PHE LYS ASP LYS THR ILE SER SER          
SEQRES   4 A  124  SER LEU ALA VAL VAL ASP LEU ILE ASP ALA ILE GLN PRO          
SEQRES   5 A  124  GLY CYS ILE ASN TYR ASP LEU VAL LYS SER GLY ASN LEU          
SEQRES   6 A  124  THR GLU ASP ASP LYS HIS ASN ASN ALA LYS TYR ALA VAL          
SEQRES   7 A  124  SER MET ALA ARG ARG ILE GLY ALA ARG VAL TYR ALA LEU          
SEQRES   8 A  124  PRO GLU ASP LEU VAL GLU VAL LYS PRO LYS MET VAL MET          
SEQRES   9 A  124  THR VAL PHE ALA CYS LEU MET GLY ARG GLY MET LYS ARG          
SEQRES  10 A  124  VAL SER GLY PRO SER SER GLY                                  
HELIX    1   1 ASP A    9  GLY A   24  1                                  16    
HELIX    2   2 THR A   36  SER A   39  5                                   4    
HELIX    3   3 SER A   40  GLN A   51  1                                  12    
HELIX    4   4 THR A   66  ILE A   84  1                                  19    
HELIX    5   5 LEU A   91  VAL A   98  1                                   8    
HELIX    6   6 MET A  104  GLY A  114  1                                  11    
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1                                                                  
(ATOM LINES ARE NOT SHOWN.)
END                                                                             
DBGET integrated database retrieval system